Click here to switch to the map view.

The map label for this gene is bcfG [H]

Identifier: 16763417

GI number: 16763417

Start: 30478

End: 31209

Strand: Direct

Name: bcfG [H]

Synonym: STM0027

Alternate gene names: 16763417

Gene position: 30478-31209 (Clockwise)

Preceding gene: 16763416

Following gene: 16763418

Centisome position: 0.63

GC content: 53.96

Gene sequence:

>732_bases
ATGCCTCCGCAACCTTCATTCTTGCCTATGAATAAGTTCTTTTTACGCTGCGCGATATATTGGTGCTTGCTTCCCATATC
ATGGGCGCAGGCTGGCGTGGTAATTGGCGGTACTCGCTTTATCTATCATGCGGGCGCCCCGGCATTAAGCGTACCGGTAA
GTAACCGTTCAGAAGCGTCCTGGTTAATTGATACGCATATTTTACCTGGTGGCCGCTGGCCAGGAACCAAAAACGAAGGG
AATATCATGCCCTTCGTGGTCACGCCGCCATTATTTATGCTTTCAGCGCGCCAGGAAAACTCAATGCGGGTGGTTTATAC
CGGCGCACCGCTACCCGCCGACAGGGAAAGTCTGTTTACCTTGAGTATCGCCGCTATTCCCTCCGGTAAACCGGAAGCGA
ACCGTGTACAAATGGCGTTTCGTTCGGCTTTAAAATTGCTCTATCGCCCTGATGGACTCGCCGGCAATCCGCAGCAGGCC
TACCGGCATCTGATCTGGTCGCTTACCCCCGATGGCGCGACGGTACGCAATCCTACACCCTATTACGTTACGCTCTTTTT
ATTACGTGCTAACGAGCGCGCCCAGGACAACGCCGGGGTCGTGGCTCCCTTTGCAACGCGTCAAACGGACTGGTGCCGGC
ACACGGTTCGCTGCACTGTGCGCTGGCAAAGTATTAATGACTATGGGCGGGTAATGCCAGCGCAAACCGTGGATCTGACG
CGTATTCATTAA

Upstream 100 bases:

>100_bases
AGTGATGTGGTGACGCTCAGTCCGGGTCAGGCGTCGGCGCATCTGCAATTTTACGCGCGTTATCTTGCCGATGGCGGCGC
GGTAACGCCCGGGGACGCCA

Downstream 100 bases:

>100_bases
CCTATTTTTCAGGCGTCTCCCGATAGCGGGAGGCTTTCCGAACTTATCGAACGAGACTTTTATTATGTATTATCACGCGT
TAAAACTTTCCCGACTGGCG

Product: fimbrial chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MPPQPSFLPMNKFFLRCAIYWCLLPISWAQAGVVIGGTRFIYHAGAPALSVPVSNRSEASWLIDTHILPGGRWPGTKNEG
NIMPFVVTPPLFMLSARQENSMRVVYTGAPLPADRESLFTLSIAAIPSGKPEANRVQMAFRSALKLLYRPDGLAGNPQQA
YRHLIWSLTPDGATVRNPTPYYVTLFLLRANERAQDNAGVVAPFATRQTDWCRHTVRCTVRWQSINDYGRVMPAQTVDLT
RIH

Sequences:

>Translated_243_residues
MPPQPSFLPMNKFFLRCAIYWCLLPISWAQAGVVIGGTRFIYHAGAPALSVPVSNRSEASWLIDTHILPGGRWPGTKNEG
NIMPFVVTPPLFMLSARQENSMRVVYTGAPLPADRESLFTLSIAAIPSGKPEANRVQMAFRSALKLLYRPDGLAGNPQQA
YRHLIWSLTPDGATVRNPTPYYVTLFLLRANERAQDNAGVVAPFATRQTDWCRHTVRCTVRWQSINDYGRVMPAQTVDLT
RIH
>Mature_242_residues
PPQPSFLPMNKFFLRCAIYWCLLPISWAQAGVVIGGTRFIYHAGAPALSVPVSNRSEASWLIDTHILPGGRWPGTKNEGN
IMPFVVTPPLFMLSARQENSMRVVYTGAPLPADRESLFTLSIAAIPSGKPEANRVQMAFRSALKLLYRPDGLAGNPQQAY
RHLIWSLTPDGATVRNPTPYYVTLFLLRANERAQDNAGVVAPFATRQTDWCRHTVRCTVRWQSINDYGRVMPAQTVDLTR
IH

Specific function: Could be required for the biogenesis of a putative fimbria [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI87081806, Length=230, Percent_Identity=41.304347826087, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787171, Length=227, Percent_Identity=33.0396475770925, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790771, Length=228, Percent_Identity=34.6491228070175, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786743, Length=234, Percent_Identity=33.7606837606838, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1786333, Length=226, Percent_Identity=32.7433628318584, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789532, Length=215, Percent_Identity=31.1627906976744, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788428, Length=204, Percent_Identity=28.4313725490196, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788677, Length=217, Percent_Identity=29.4930875576037, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786936, Length=204, Percent_Identity=28.921568627451, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 27149; Mature: 27017

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPQPSFLPMNKFFLRCAIYWCLLPISWAQAGVVIGGTRFIYHAGAPALSVPVSNRSEAS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECEEEEEECCCCEEEECCCCCCCCE
WLIDTHILPGGRWPGTKNEGNIMPFVVTPPLFMLSARQENSMRVVYTGAPLPADRESLFT
EEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEEEEEECCCCCCCCCCEEE
LSIAAIPSGKPEANRVQMAFRSALKLLYRPDGLAGNPQQAYRHLIWSLTPDGATVRNPTP
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCC
YYVTLFLLRANERAQDNAGVVAPFATRQTDWCRHTVRCTVRWQSINDYGRVMPAQTVDLT
EEEEEEEEECCCCCCCCCCEEEECCCCCCHHHCEEEEEEEEEECCCCCCCCCCCCCEEEE
RIH
ECC
>Mature Secondary Structure 
PPQPSFLPMNKFFLRCAIYWCLLPISWAQAGVVIGGTRFIYHAGAPALSVPVSNRSEAS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECEEEEEECCCCEEEECCCCCCCCE
WLIDTHILPGGRWPGTKNEGNIMPFVVTPPLFMLSARQENSMRVVYTGAPLPADRESLFT
EEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEEEEEECCCCCCCCCCEEE
LSIAAIPSGKPEANRVQMAFRSALKLLYRPDGLAGNPQQAYRHLIWSLTPDGATVRNPTP
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCC
YYVTLFLLRANERAQDNAGVVAPFATRQTDWCRHTVRCTVRWQSINDYGRVMPAQTVDLT
EEEEEEEEECCCCCCCCCCEEEECCCCCCHHHCEEEEEEEEEECCCCCCCCCCCCCEEEE
RIH
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2992959; 7984428 [H]