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The map label for this gene is nrfC [H]

Identifier: 167625914

GI number: 167625914

Start: 4884919

End: 4885614

Strand: Reverse

Name: nrfC [H]

Synonym: Shal_4009

Alternate gene names: 167625914

Gene position: 4885614-4884919 (Counterclockwise)

Preceding gene: 167625930

Following gene: 167625913

Centisome position: 93.47

GC content: 45.83

Gene sequence:

>696_bases
ATGTTGGATAGACGCTCCATATTAAAAATGGCTGCTGGTGGTGCAATGTTAACCCTCATCCCTAGTGCTCAACTTCAAGC
TAAGACACCTGATAAACGATTGGCCATGGTGTTTGATGTTCGCCGTTGCACAGGCTGTCTATCTTGCACTGTGTCTTGTG
CGATGGAAAATCAGACCGATGCGGGCCGCTGTCGAACTCGGGTTAATCAGGCTGCGATTGAAACTGAATTGGGGTTGGCC
ACACTCGCAGTTCCTAATCAATGTAATCAATGTGATAACCCCGCTTGCGTCTATGTTTGCCCAGTCGAAGCGACCTATAA
GCGTAAAGAAGATGGCATCGTCGTGATCAATCATGATGAATGCATTCACTGCCAGTTGTGTGTCGATGCCTGCCCTTATG
GTGCACGCCGTAAAGATGAAACTCTTAACTCTCCACCTGAGAAATGTAACTTCTGTATTCATCGTGTATCGGTAGGCTTG
CTACCAGCTTGTGTGGAAACTTGTATTGGGGAAGCGCGTAACTTTGGTGATCTTAATGATCCTAATAGCAAGGTTTCTCA
ATTGGTTCGCGACAACAAGGTATACGCCATGTTAGCCGATGCCGGTACTAAGCCAAATATCTTCTATATCGGCTTACCGG
AACAACCTAAAGATAAACAAATTTTGTCACTTAACTTCCTCGAATGGCAACGCTAG

Upstream 100 bases:

>100_bases
CGCCTTTGTTTATAACTTTATATTTTTGAGTGTATTTTAAATATAAAGTTTTTATAAGTGATATCAGTAATATTTACTGT
GTTCATAATAAGGAATAATT

Downstream 100 bases:

>100_bases
GAGCTGATAATGGATAGACGTAAATTTTTAACCCAAGCGGGCGTTGTCTCTGTCGCGGCGGCAACTGTGGGTTGTAGTGA
AAAAACTCAACCAATACATC

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MLDRRSILKMAAGGAMLTLIPSAQLQAKTPDKRLAMVFDVRRCTGCLSCTVSCAMENQTDAGRCRTRVNQAAIETELGLA
TLAVPNQCNQCDNPACVYVCPVEATYKRKEDGIVVINHDECIHCQLCVDACPYGARRKDETLNSPPEKCNFCIHRVSVGL
LPACVETCIGEARNFGDLNDPNSKVSQLVRDNKVYAMLADAGTKPNIFYIGLPEQPKDKQILSLNFLEWQR

Sequences:

>Translated_231_residues
MLDRRSILKMAAGGAMLTLIPSAQLQAKTPDKRLAMVFDVRRCTGCLSCTVSCAMENQTDAGRCRTRVNQAAIETELGLA
TLAVPNQCNQCDNPACVYVCPVEATYKRKEDGIVVINHDECIHCQLCVDACPYGARRKDETLNSPPEKCNFCIHRVSVGL
LPACVETCIGEARNFGDLNDPNSKVSQLVRDNKVYAMLADAGTKPNIFYIGLPEQPKDKQILSLNFLEWQR
>Mature_231_residues
MLDRRSILKMAAGGAMLTLIPSAQLQAKTPDKRLAMVFDVRRCTGCLSCTVSCAMENQTDAGRCRTRVNQAAIETELGLA
TLAVPNQCNQCDNPACVYVCPVEATYKRKEDGIVVINHDECIHCQLCVDACPYGARRKDETLNSPPEKCNFCIHRVSVGL
LPACVETCIGEARNFGDLNDPNSKVSQLVRDNKVYAMLADAGTKPNIFYIGLPEQPKDKQILSLNFLEWQR

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=180, Percent_Identity=36.1111111111111, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787122, Length=183, Percent_Identity=34.4262295081967, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI226510944, Length=210, Percent_Identity=29.0476190476191, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787872, Length=183, Percent_Identity=33.879781420765, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1790326, Length=239, Percent_Identity=27.6150627615063, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1787478, Length=128, Percent_Identity=35.9375, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1787740, Length=97, Percent_Identity=42.2680412371134, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787749, Length=257, Percent_Identity=27.2373540856031, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789370, Length=211, Percent_Identity=31.7535545023697, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082180, Length=164, Percent_Identity=27.4390243902439, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI2367245, Length=159, Percent_Identity=32.7044025157233, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25500; Mature: 25500

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
10.0 %Cys+Met (Translated Protein)
7.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
10.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRRSILKMAAGGAMLTLIPSAQLQAKTPDKRLAMVFDVRRCTGCLSCTVSCAMENQTD
CCCHHHHHHHHCCCEEEEEECCCCEECCCCCHHHHHEEHHHHHHHHHHHHEEEEECCCCC
AGRCRTRVNQAAIETELGLATLAVPNQCNQCDNPACVYVCPVEATYKRKEDGIVVINHDE
HHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEECCCHHCCCCCCCEEEEECCC
CIHCQLCVDACPYGARRKDETLNSPPEKCNFCIHRVSVGLLPACVETCIGEARNFGDLND
EEEEEEEEHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PNSKVSQLVRDNKVYAMLADAGTKPNIFYIGLPEQPKDKQILSLNFLEWQR
CHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEEECEECCC
>Mature Secondary Structure
MLDRRSILKMAAGGAMLTLIPSAQLQAKTPDKRLAMVFDVRRCTGCLSCTVSCAMENQTD
CCCHHHHHHHHCCCEEEEEECCCCEECCCCCHHHHHEEHHHHHHHHHHHHEEEEECCCCC
AGRCRTRVNQAAIETELGLATLAVPNQCNQCDNPACVYVCPVEATYKRKEDGIVVINHDE
HHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEECCCHHCCCCCCCEEEEECCC
CIHCQLCVDACPYGARRKDETLNSPPEKCNFCIHRVSVGLLPACVETCIGEARNFGDLND
EEEEEEEEHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PNSKVSQLVRDNKVYAMLADAGTKPNIFYIGLPEQPKDKQILSLNFLEWQR
CHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEEECEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]