Click here to switch to the map view.

The map label for this gene is outD [H]

Identifier: 167625758

GI number: 167625758

Start: 4674681

End: 4676354

Strand: Reverse

Name: outD [H]

Synonym: Shal_3853

Alternate gene names: 167625758

Gene position: 4676354-4674681 (Counterclockwise)

Preceding gene: 167625759

Following gene: 167625757

Centisome position: 89.47

GC content: 45.52

Gene sequence:

>1674_bases
ATGCGCTCAATAATCTATATCACCCCTTTGCTGTCATTGTTCTTAGTGGCTTGCCAAACTGTCGACAGGCCTAATCCTGT
AGCCTCTAAAGAGGCGTTAGCTGAGTCTATCGCCGCAGATAAAGCCGCAAATGCGACGCCGCCGCCAGCCGTCATGCCTG
AAGATGTTCAGCGAGAGTTGTCATCCGATAACTTGTTGCAAGGTTTTGCACCAGCGAGACCGACTGAGCGACGTTTCGAT
GTATCCGCTAACAATGTCGATGCTAAAGTCTTTTTCCCTAGCCTGGCACAAGGCACTCCGTTAAGTGTTGCGGTGCACCC
TGATGTAACGGGCAAAATTTCATTGTCTTTAAAAAGCGTTACGTTGAAAGAAGCCTTACAAGTCGTTGAAGATATCTACG
GTTATGAAGTCAGCCGAGAAGGGCGCATATTACGTATTTTTCCTGCGGGGATGCGCACCGAAACCTTTTCGTTAAATTAT
CTGTACATGGAGCGCCAAGGTCTGTCTTTGACTTCGGTGAGTTCAGGTCGCATTTCAGATAATGACTCAAACTCTAATAG
CAACAGCAATAGTAGCGGCAACAGCAATAGTAATTTCAATAACTCGTCTAATAGTGACTCTGGCTCTAGTAACAGTTCAA
GTGATCAAACTAACGGTACCTTTATTCGCTCTGAGACGAAATCCGATTTTTGGGGTGAGTTAAAAGAGACCTTAGTCTCA
ATCATGGGTAATACCGGTGGTGGTCGCCAAGTGGTGGTAACGCCGCAAGCGGGTTTAGTCACAGTTAGAGCCTATCCAAA
TGAGATCCGCCAGATCCGTAACTTTATAAAGACAGCTGAAAGTCATCTGCAGCGCCAAGTCATCTTGGAAGCCAAAGTAC
TCGAAGTGACTTTGTCTGATGGCTATCAGCAAGGGATCCACTGGGAAAGTGTACTCGGTCATGCAGGCAGTACCGATATC
ACCTTTGGTACCAGCCCTACGACAGGTCTTAGCGATCAAATCTCTAGCGTGTTAGGGGGAGTGACATCGATTAAGCTGGA
AGGATCAGACTTTAGCACCATGATTAGTCTGCTTGATACTCAAGGTGACGTTGATGTGTTATCAAGCCCACGAGTAACCG
CATCTAATAACCAGAAAGCGGTTATTAAGGTCGGTACCGATGAGTACTTTGTGACTAACGTATCTTCTACAACAGTGGCA
AGTAATACCCCAATAACCACTCCTGATGTGGAATTGACGCCATTCTTCTCTGGTATCGCACTTGATGTTACGCCGCAAAT
TGATGAGCAAGGCAATGTGCTGCTGCATATTCACCCCTCTGTGATTGATATTAAAGAGCAAACTAAGATTATTAAGATCT
CTGGTAGTACATTGGAGTTACCTTTGGCGCAGAGTGAGATCCGCGAATCAGATACCGTGATCAAGGCCGCTAGCGGTGAT
GTTGTGGTGATTGGCGGTTTGATGAAGAGTGAAAATACTGAGATTGTCTCTAAAGTCCCATTACTTGGTGATATCCCATT
CCTAGGAGAAGCTTTCACTAACCGTGCAAACTCAACCGTTAAGACAGAGTTAGTTATTTTGCTTAAACCAATAGTTGTCG
GTGCTAACACTTGGGAAAACGAGTTGAAGCGTTCTCAAGAGCTACTTGATCGTTGGTATCCTGAGGAGCAATAA

Upstream 100 bases:

>100_bases
TCAAAATCGTGCATATAGGTAAAGACACAGTGTCGTTAGCGGACGGTCGTAAGTTGACTTTGTTTCCAGCTGTAACAGAT
CTTAAGGGACAATAATTAAA

Downstream 100 bases:

>100_bases
GCTTGTATCTGCAGCATTTTGGGTTAACAGAAACCCCGTTTTCTCTTACACCGAACACGGGATTTTTCTTTGGCCTCGCA
CCGCATGTAGAAGCTCTACA

Product: pilus (MSHA type) biogenesis protein MshL

Products: NA

Alternate protein names: Pectic enzymes secretion protein outD [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MRSIIYITPLLSLFLVACQTVDRPNPVASKEALAESIAADKAANATPPPAVMPEDVQRELSSDNLLQGFAPARPTERRFD
VSANNVDAKVFFPSLAQGTPLSVAVHPDVTGKISLSLKSVTLKEALQVVEDIYGYEVSREGRILRIFPAGMRTETFSLNY
LYMERQGLSLTSVSSGRISDNDSNSNSNSNSSGNSNSNFNNSSNSDSGSSNSSSDQTNGTFIRSETKSDFWGELKETLVS
IMGNTGGGRQVVVTPQAGLVTVRAYPNEIRQIRNFIKTAESHLQRQVILEAKVLEVTLSDGYQQGIHWESVLGHAGSTDI
TFGTSPTTGLSDQISSVLGGVTSIKLEGSDFSTMISLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTNVSSTTVA
SNTPITTPDVELTPFFSGIALDVTPQIDEQGNVLLHIHPSVIDIKEQTKIIKISGSTLELPLAQSEIRESDTVIKAASGD
VVVIGGLMKSENTEIVSKVPLLGDIPFLGEAFTNRANSTVKTELVILLKPIVVGANTWENELKRSQELLDRWYPEEQ

Sequences:

>Translated_557_residues
MRSIIYITPLLSLFLVACQTVDRPNPVASKEALAESIAADKAANATPPPAVMPEDVQRELSSDNLLQGFAPARPTERRFD
VSANNVDAKVFFPSLAQGTPLSVAVHPDVTGKISLSLKSVTLKEALQVVEDIYGYEVSREGRILRIFPAGMRTETFSLNY
LYMERQGLSLTSVSSGRISDNDSNSNSNSNSSGNSNSNFNNSSNSDSGSSNSSSDQTNGTFIRSETKSDFWGELKETLVS
IMGNTGGGRQVVVTPQAGLVTVRAYPNEIRQIRNFIKTAESHLQRQVILEAKVLEVTLSDGYQQGIHWESVLGHAGSTDI
TFGTSPTTGLSDQISSVLGGVTSIKLEGSDFSTMISLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTNVSSTTVA
SNTPITTPDVELTPFFSGIALDVTPQIDEQGNVLLHIHPSVIDIKEQTKIIKISGSTLELPLAQSEIRESDTVIKAASGD
VVVIGGLMKSENTEIVSKVPLLGDIPFLGEAFTNRANSTVKTELVILLKPIVVGANTWENELKRSQELLDRWYPEEQ
>Mature_557_residues
MRSIIYITPLLSLFLVACQTVDRPNPVASKEALAESIAADKAANATPPPAVMPEDVQRELSSDNLLQGFAPARPTERRFD
VSANNVDAKVFFPSLAQGTPLSVAVHPDVTGKISLSLKSVTLKEALQVVEDIYGYEVSREGRILRIFPAGMRTETFSLNY
LYMERQGLSLTSVSSGRISDNDSNSNSNSNSSGNSNSNFNNSSNSDSGSSNSSSDQTNGTFIRSETKSDFWGELKETLVS
IMGNTGGGRQVVVTPQAGLVTVRAYPNEIRQIRNFIKTAESHLQRQVILEAKVLEVTLSDGYQQGIHWESVLGHAGSTDI
TFGTSPTTGLSDQISSVLGGVTSIKLEGSDFSTMISLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTNVSSTTVA
SNTPITTPDVELTPFFSGIALDVTPQIDEQGNVLLHIHPSVIDIKEQTKIIKISGSTLELPLAQSEIRESDTVIKAASGD
VVVIGGLMKSENTEIVSKVPLLGDIPFLGEAFTNRANSTVKTELVILLKPIVVGANTWENELKRSQELLDRWYPEEQ

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=291, Percent_Identity=27.4914089347079, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789793, Length=251, Percent_Identity=26.6932270916335, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 60073; Mature: 60073

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIIYITPLLSLFLVACQTVDRPNPVASKEALAESIAADKAANATPPPAVMPEDVQREL
CCEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHC
SSDNLLQGFAPARPTERRFDVSANNVDAKVFFPSLAQGTPLSVAVHPDVTGKISLSLKSV
CCCCHHHCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEEEEHH
TLKEALQVVEDIYGYEVSREGRILRIFPAGMRTETFSLNYLYMERQGLSLTSVSSGRISD
HHHHHHHHHHHHHCCEECCCCCEEEEECCCCCEEEEEEEEEEEECCCCEEEECCCCCCCC
NDSNSNSNSNSSGNSNSNFNNSSNSDSGSSNSSSDQTNGTFIRSETKSDFWGELKETLVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
IMGNTGGGRQVVVTPQAGLVTVRAYPNEIRQIRNFIKTAESHLQRQVILEAKVLEVTLSD
HHCCCCCCCEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECC
GYQQGIHWESVLGHAGSTDITFGTSPTTGLSDQISSVLGGVTSIKLEGSDFSTMISLLDT
CHHHCCCHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHC
QGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTNVSSTTVASNTPITTPDVELTPFFSGIA
CCCEEECCCCCEEECCCCEEEEEECCCCEEEEECCCCEEECCCCCCCCCCEECCCCCCEE
LDVTPQIDEQGNVLLHIHPSVIDIKEQTKIIKISGSTLELPLAQSEIRESDTVIKAASGD
EEECCCCCCCCCEEEEECCCEEEECCCCEEEEEECCEEEECCCHHHHHCCCCEEEECCCC
VVVIGGLMKSENTEIVSKVPLLGDIPFLGEAFTNRANSTVKTELVILLKPIVVGANTWEN
EEEEECEECCCCCHHHHHCCCCCCCCCHHHHHHCCCCCCHHHEEEEEEEEEEECCCCHHH
ELKRSQELLDRWYPEEQ
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRSIIYITPLLSLFLVACQTVDRPNPVASKEALAESIAADKAANATPPPAVMPEDVQREL
CCEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHC
SSDNLLQGFAPARPTERRFDVSANNVDAKVFFPSLAQGTPLSVAVHPDVTGKISLSLKSV
CCCCHHHCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEEEEHH
TLKEALQVVEDIYGYEVSREGRILRIFPAGMRTETFSLNYLYMERQGLSLTSVSSGRISD
HHHHHHHHHHHHHCCEECCCCCEEEEECCCCCEEEEEEEEEEEECCCCEEEECCCCCCCC
NDSNSNSNSNSSGNSNSNFNNSSNSDSGSSNSSSDQTNGTFIRSETKSDFWGELKETLVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
IMGNTGGGRQVVVTPQAGLVTVRAYPNEIRQIRNFIKTAESHLQRQVILEAKVLEVTLSD
HHCCCCCCCEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECC
GYQQGIHWESVLGHAGSTDITFGTSPTTGLSDQISSVLGGVTSIKLEGSDFSTMISLLDT
CHHHCCCHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHC
QGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTNVSSTTVASNTPITTPDVELTPFFSGIA
CCCEEECCCCCEEECCCCEEEEEECCCCEEEEECCCCEEECCCCCCCCCCEECCCCCCEE
LDVTPQIDEQGNVLLHIHPSVIDIKEQTKIIKISGSTLELPLAQSEIRESDTVIKAASGD
EEECCCCCCCCCEEEEECCCEEEECCCCEEEEEECCEEEECCCHHHHHCCCCEEEECCCC
VVVIGGLMKSENTEIVSKVPLLGDIPFLGEAFTNRANSTVKTELVILLKPIVVGANTWEN
EEEEECEECCCCCHHHHHCCCCCCCCCHHHHHHCCCCCCHHHEEEEEEEEEEECCCCHHH
ELKRSQELLDRWYPEEQ
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]