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The map label for this gene is nudF [H]

Identifier: 167625562

GI number: 167625562

Start: 4456472

End: 4457083

Strand: Reverse

Name: nudF [H]

Synonym: Shal_3656

Alternate gene names: 167625562

Gene position: 4457083-4456472 (Counterclockwise)

Preceding gene: 167625564

Following gene: 167625561

Centisome position: 85.27

GC content: 45.92

Gene sequence:

>612_bases
ATGAACAGCACATTTAATCGTGATGACGTGAAACTTTTGAGCAAAAATACCTTATTTAAAGGTTTTTTTAAGATGGATGA
GTACCGCTTTAAACACCGATTGTTTAATGGTGGCTGGAGTGATGAGGTGTCACGTGAGGTATTTGAACGTGGCCATGCCG
TGGTTGTCTTACCCTACGACCCCATTTCGGATCAGGTGGTATTGATTGAGCAGATCCGTATTCCCGTACTTGAAAGTGGT
AAAAACCCTTGGTTACTCGAACTCGTGGCGGGGATGATCGACAGCGGACAGCTTTCGGCCGATGTTGCCCATCGTGAGCT
TAAGGAGGAGGCCGGCTTGCACGCGCAACAATTAACGAAAGTGAATGAATACTTCTCAAGTCCGGGTGGAGCCAGCGAGC
GTTTTGACTTTTATTGGGCTAAGATCTGTGCCGCAGAGGCAAAAGGGGTTCATGGCTTAGAGCATGAACAAGAAGATATT
CGAGTTCATGTATTGAGCCGAGAGCAAGCCTATCAAATGGTGGTATCGGGGCGAATTGATAATGCGTCTACCGTAATAGG
CCTGCTTTGGCTGCAATTAAATTATCAAGATATCATTAAGCTGAACGGTTAG

Upstream 100 bases:

>100_bases
ATCATTATGATTAGTACTGCTTTCATCATAGTATTAATATTTTTGTGTGAGTTGATTGTGGTATGAAAACCTTTATAAAC
AACTATGTTTAGGATTGGAA

Downstream 100 bases:

>100_bases
AAGTAAACAGCCTTAGTGGTGAAATGAAGTTAGAAAGAGTTGGAACTGAGCCGTGTCAAAAGTCGTCTTTGCAAGAAAAA
ATAAGTATCAGCCTAATGTG

Product: NUDIX hydrolase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MNSTFNRDDVKLLSKNTLFKGFFKMDEYRFKHRLFNGGWSDEVSREVFERGHAVVVLPYDPISDQVVLIEQIRIPVLESG
KNPWLLELVAGMIDSGQLSADVAHRELKEEAGLHAQQLTKVNEYFSSPGGASERFDFYWAKICAAEAKGVHGLEHEQEDI
RVHVLSREQAYQMVVSGRIDNASTVIGLLWLQLNYQDIIKLNG

Sequences:

>Translated_203_residues
MNSTFNRDDVKLLSKNTLFKGFFKMDEYRFKHRLFNGGWSDEVSREVFERGHAVVVLPYDPISDQVVLIEQIRIPVLESG
KNPWLLELVAGMIDSGQLSADVAHRELKEEAGLHAQQLTKVNEYFSSPGGASERFDFYWAKICAAEAKGVHGLEHEQEDI
RVHVLSREQAYQMVVSGRIDNASTVIGLLWLQLNYQDIIKLNG
>Mature_203_residues
MNSTFNRDDVKLLSKNTLFKGFFKMDEYRFKHRLFNGGWSDEVSREVFERGHAVVVLPYDPISDQVVLIEQIRIPVLESG
KNPWLLELVAGMIDSGQLSADVAHRELKEEAGLHAQQLTKVNEYFSSPGGASERFDFYWAKICAAEAKGVHGLEHEQEDI
RVHVLSREQAYQMVVSGRIDNASTVIGLLWLQLNYQDIIKLNG

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=193, Percent_Identity=56.4766839378238, Blast_Score=233, Evalue=5e-63,
Organism=Escherichia coli, GI1788810, Length=152, Percent_Identity=32.2368421052632, Blast_Score=74, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23108; Mature: 23108

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTFNRDDVKLLSKNTLFKGFFKMDEYRFKHRLFNGGWSDEVSREVFERGHAVVVLPYD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECC
PISDQVVLIEQIRIPVLESGKNPWLLELVAGMIDSGQLSADVAHRELKEEAGLHAQQLTK
CCCCCEEEEEHHCCCCEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHH
VNEYFSSPGGASERFDFYWAKICAAEAKGVHGLEHEQEDIRVHVLSREQAYQMVVSGRID
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCC
NASTVIGLLWLQLNYQDIIKLNG
CHHHHHHHHHEEECHHHEEEECC
>Mature Secondary Structure
MNSTFNRDDVKLLSKNTLFKGFFKMDEYRFKHRLFNGGWSDEVSREVFERGHAVVVLPYD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECC
PISDQVVLIEQIRIPVLESGKNPWLLELVAGMIDSGQLSADVAHRELKEEAGLHAQQLTK
CCCCCEEEEEHHCCCCEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHH
VNEYFSSPGGASERFDFYWAKICAAEAKGVHGLEHEQEDIRVHVLSREQAYQMVVSGRID
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCC
NASTVIGLLWLQLNYQDIIKLNG
CHHHHHHHHHEEECHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]