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The map label for this gene is cobS

Identifier: 167625367

GI number: 167625367

Start: 4210523

End: 4211302

Strand: Reverse

Name: cobS

Synonym: Shal_3456

Alternate gene names: 167625367

Gene position: 4211302-4210523 (Counterclockwise)

Preceding gene: 167625368

Following gene: 167625366

Centisome position: 80.57

GC content: 49.23

Gene sequence:

>780_bases
ATGCAACTTTGGCAAAAACAGCTCAATCTATTTTTTATCGCAATGGGATTCTTTACTCGGATCCCAATGCCGAAGTGGAT
TGAGGTTGATGCAGATAAACTCAATAAAGCGAGCCGCTACTTCGGTTTAGTGGGCTTGCTGGTGGGGGCAATTAGTGCTG
CGGTTTACAGCCTAATGCTGTATTGGGTCTCGCCCTCTGTTGCGATCGTGTTTGCCATGATAACCAGCGTGTTAGTCACG
GGTGGCTTTCATGAAGATGGTCTTGCCGATACCGCTGACGGTCTTGGTGGCGGCTGGACGGTAGAAGCTAAACTTAACAT
CATGAAAGACTCACGCCTTGGTAGTTATGGCGCGCTGGCTTTGGTACTGGCCCTGCTACTCAAGTGGCAATTGCTGACAG
AGTTAGCCCTGTTTGACCCGAGCTCTGTGAGTCTGGCGTTAATCGTTGGGCATTGTTTGAGCCGAGTGGTGGCAGCGAGT
TTTATCTTTTCAGAGGAATATGTCAGTGACACTGATACCAGTAAGAGCAAGCCTTTGGCCCAGCAGCAGGGGATCAATGA
GTTATCGATCTTACTGGCCTCCGGCGTGTTGGCACTGCTGTTAGTTGGTTTAGTGCCTGCATTAGTGCTGATTACGGGTC
TTGTTATTGTGCGTTACGGCTTTATTAGATTGTTTCGAAGCCAGATTGGTGGCTATACCGGAGACACACTTGGCGCTGCC
CAGCAAGGCTCGGAGCTTAGCTGTTATCTATTATTACTCATCTTAGGAGTCAGCTGGTGA

Upstream 100 bases:

>100_bases
AACCGTCTCTGCTCTCTCTGACTCTAATCGTCTTTGCTTTATCCGGCTTTTATCGGCTCTCCCCGTCTTAGCCTTATTCG
ATTTTTTTAAGGATTTTTAC

Downstream 100 bases:

>100_bases
TCCATCTGGTATTAGGTGGTGCTCGCAGCGGTAAGAGCCGCTTCGCCGAGGCGCAAGTAGCTGAGCTGCAAAACAATGTT
GATAGCGAGTGCTTCTATTT

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MQLWQKQLNLFFIAMGFFTRIPMPKWIEVDADKLNKASRYFGLVGLLVGAISAAVYSLMLYWVSPSVAIVFAMITSVLVT
GGFHEDGLADTADGLGGGWTVEAKLNIMKDSRLGSYGALALVLALLLKWQLLTELALFDPSSVSLALIVGHCLSRVVAAS
FIFSEEYVSDTDTSKSKPLAQQQGINELSILLASGVLALLLVGLVPALVLITGLVIVRYGFIRLFRSQIGGYTGDTLGAA
QQGSELSCYLLLLILGVSW

Sequences:

>Translated_259_residues
MQLWQKQLNLFFIAMGFFTRIPMPKWIEVDADKLNKASRYFGLVGLLVGAISAAVYSLMLYWVSPSVAIVFAMITSVLVT
GGFHEDGLADTADGLGGGWTVEAKLNIMKDSRLGSYGALALVLALLLKWQLLTELALFDPSSVSLALIVGHCLSRVVAAS
FIFSEEYVSDTDTSKSKPLAQQQGINELSILLASGVLALLLVGLVPALVLITGLVIVRYGFIRLFRSQIGGYTGDTLGAA
QQGSELSCYLLLLILGVSW
>Mature_259_residues
MQLWQKQLNLFFIAMGFFTRIPMPKWIEVDADKLNKASRYFGLVGLLVGAISAAVYSLMLYWVSPSVAIVFAMITSVLVT
GGFHEDGLADTADGLGGGWTVEAKLNIMKDSRLGSYGALALVLALLLKWQLLTELALFDPSSVSLALIVGHCLSRVVAAS
FIFSEEYVSDTDTSKSKPLAQQQGINELSILLASGVLALLLVGLVPALVLITGLVIVRYGFIRLFRSQIGGYTGDTLGAA
QQGSELSCYLLLLILGVSW

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=252, Percent_Identity=35.7142857142857, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_SHEHH (B0TSP8)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001675661.1
- GeneID:   5903624
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_3456
- HOGENOM:   HBG729392
- OMA:   HDSRIGA
- ProtClustDB:   CLSK2516811
- BioCyc:   SHAL458817:SHAL_3456-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27882; Mature: 27882

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xff501c0)-; HASH(0x1093e9e4)-; HASH(0x1118c710)-; HASH(0x10ccbe48)-; HASH(0x1120fe58)-; HASH(0x1170665c)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLWQKQLNLFFIAMGFFTRIPMPKWIEVDADKLNKASRYFGLVGLLVGAISAAVYSLML
CCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YWVSPSVAIVFAMITSVLVTGGFHEDGLADTADGLGGGWTVEAKLNIMKDSRLGSYGALA
HHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEHHCCCCCCHHHHH
LVLALLLKWQLLTELALFDPSSVSLALIVGHCLSRVVAASFIFSEEYVSDTDTSKSKPLA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
QQQGINELSILLASGVLALLLVGLVPALVLITGLVIVRYGFIRLFRSQIGGYTGDTLGAA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
QQGSELSCYLLLLILGVSW
CCCCCHHHHHHHHHHHCCH
>Mature Secondary Structure
MQLWQKQLNLFFIAMGFFTRIPMPKWIEVDADKLNKASRYFGLVGLLVGAISAAVYSLML
CCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YWVSPSVAIVFAMITSVLVTGGFHEDGLADTADGLGGGWTVEAKLNIMKDSRLGSYGALA
HHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEHHCCCCCCHHHHH
LVLALLLKWQLLTELALFDPSSVSLALIVGHCLSRVVAASFIFSEEYVSDTDTSKSKPLA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
QQQGINELSILLASGVLALLLVGLVPALVLITGLVIVRYGFIRLFRSQIGGYTGDTLGAA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
QQGSELSCYLLLLILGVSW
CCCCCHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA