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The map label for this gene is hyfR [H]

Identifier: 167625357

GI number: 167625357

Start: 4197560

End: 4199086

Strand: Reverse

Name: hyfR [H]

Synonym: Shal_3446

Alternate gene names: 167625357

Gene position: 4199086-4197560 (Counterclockwise)

Preceding gene: 167625361

Following gene: 167625348

Centisome position: 80.34

GC content: 47.28

Gene sequence:

>1527_bases
ATGAAAGAACAATTTTATTATCAGGCTACCCAGTGCTTATGTAGCAGTTTGTCGCTGGAAGTCGCGCTGCAAAACTACTA
CGAATATGTAAAACAGTACCTGCCTATAGATGGCATTTTTCTCAACATCTATCGGCCGGAGCATGCCGATATTCAGTTTC
TCGCCCATGTTAACGAACAGCAGGCCGTTGCACTCGATAAACAGGTGAAGATCTCAGCCGAAATGGAGCAGGTGCTGCAG
CAGAATGACCGCCCAGCGATCCGCATTATCAATGATATTCATCTAGATAAGGTCACCAGCTTTGTGGCACCTCAGGTGAT
CCCTGAAATCGGCTCAGTGATCTTATTGAGGATGATCAGTGACAACACCCATTTAGGTGTTGTGGGCTTTTATGCTAAGC
AAGCTGGACAGTTTAAGCCACTTCATGCCGACTTAATTGAGCCTCACAAAGATACCTTCTCCTTGATCACCGCCTTTAAC
TTACGTGGTAGAAATCTACTCAAAGCTAACCAAGTGTTAAGCCAAGAAAACGATAATCTTAAGCGCGCCATGTTTTCACC
CGATGGTGTCGTCGGGGCCGATGGAGGCCTTAAAAAGGTGATGGTACAGGTCGATGCGATTGCAGCGCTTAATACTAGCG
TGCTGATGCTAGGGGAAACGGGTTGTGGTAAAGAGGTGATCGCCAACGCGATCCATGCAAAGTCGAACCGTGCGGATAAG
CCTTTTATTAAGGTGAACTGTGGTGCGATACCGGAAACCTTGCTTGATTCAGAGTTGTTTGGCTATGAAAAGGGTGCATT
CACCGGGGCGGAAACTCGTAAGGCGGGATATTTTGAGCAAGCCAATGGTGGCACTATCTTCCTAGACGAAATTGGTGAGC
TACCGCTGTCGGCGCAAGTGCGTTTACTGCGCGTGTTGCAAAACAGCTCAATTACCCGAGTGGGTGGCCATGAGAATGTG
GATCTGAATATTCGGGTGATAGCAGCGACTCACAGAAACCTACAAGCTATGGTGCATGAGAAAACCTTTAGGGAAGATCT
CTGGTATCGCTTGTCTGTTTTTCCGATAGAGATCCCAGCGCTGCGGCAACGTAGAAGCGATATTCCACTGTTAGTACAGT
ATTTTATCGAGACATTGGCGGCTAAGTTTAATCTAGAGCAGTTACCTAGAGTGATGCCAGAGCAGTTAGCCATGCTAAAC
AATTACAACTGGCCGGGTAATGTGCGCGAGCTGATTAACGTACTCGAGCGCGCCATCATCCAAAAGCCTATGGGGCCGCT
TAGCTTCGATCTATTGCAAGCGCAACCTGATGAAAATACAACGATCAAAACAGGGCAAACCATCGTTGTCGATCCAAGTT
ACGCCAGCGATAAGTTAGTGCCACTCGATACTATGATAGGTAAGTACATCAGCCATGCGATGCGTATTACCGGTGGTAAA
CTCTATGGACCTGCTGGGGCGGCAGAGCTATTAGACATCAATCCTAATACCCTAAAGAGTAAGATGAAAAAGCTCGGACT
GTGTTAG

Upstream 100 bases:

>100_bases
TGGTTTACGTTCTGTGTTTGATTATTTATGAACAGGAACACAGAATCACGAAATACAATGATCTACCATCACGAAACTTC
GTGGCCACCTCGACATATCA

Downstream 100 bases:

>100_bases
TGTTTGCGTTAGTGCGTTAGTGCGTTAGTCATCATTAACTAATAAAAAAACACTTCCAAGGAAGTGTTTTTTGCCGAGCG
GGGGACAACAAATGTTAGGT

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MKEQFYYQATQCLCSSLSLEVALQNYYEYVKQYLPIDGIFLNIYRPEHADIQFLAHVNEQQAVALDKQVKISAEMEQVLQ
QNDRPAIRIINDIHLDKVTSFVAPQVIPEIGSVILLRMISDNTHLGVVGFYAKQAGQFKPLHADLIEPHKDTFSLITAFN
LRGRNLLKANQVLSQENDNLKRAMFSPDGVVGADGGLKKVMVQVDAIAALNTSVLMLGETGCGKEVIANAIHAKSNRADK
PFIKVNCGAIPETLLDSELFGYEKGAFTGAETRKAGYFEQANGGTIFLDEIGELPLSAQVRLLRVLQNSSITRVGGHENV
DLNIRVIAATHRNLQAMVHEKTFREDLWYRLSVFPIEIPALRQRRSDIPLLVQYFIETLAAKFNLEQLPRVMPEQLAMLN
NYNWPGNVRELINVLERAIIQKPMGPLSFDLLQAQPDENTTIKTGQTIVVDPSYASDKLVPLDTMIGKYISHAMRITGGK
LYGPAGAAELLDINPNTLKSKMKKLGLC

Sequences:

>Translated_508_residues
MKEQFYYQATQCLCSSLSLEVALQNYYEYVKQYLPIDGIFLNIYRPEHADIQFLAHVNEQQAVALDKQVKISAEMEQVLQ
QNDRPAIRIINDIHLDKVTSFVAPQVIPEIGSVILLRMISDNTHLGVVGFYAKQAGQFKPLHADLIEPHKDTFSLITAFN
LRGRNLLKANQVLSQENDNLKRAMFSPDGVVGADGGLKKVMVQVDAIAALNTSVLMLGETGCGKEVIANAIHAKSNRADK
PFIKVNCGAIPETLLDSELFGYEKGAFTGAETRKAGYFEQANGGTIFLDEIGELPLSAQVRLLRVLQNSSITRVGGHENV
DLNIRVIAATHRNLQAMVHEKTFREDLWYRLSVFPIEIPALRQRRSDIPLLVQYFIETLAAKFNLEQLPRVMPEQLAMLN
NYNWPGNVRELINVLERAIIQKPMGPLSFDLLQAQPDENTTIKTGQTIVVDPSYASDKLVPLDTMIGKYISHAMRITGGK
LYGPAGAAELLDINPNTLKSKMKKLGLC
>Mature_508_residues
MKEQFYYQATQCLCSSLSLEVALQNYYEYVKQYLPIDGIFLNIYRPEHADIQFLAHVNEQQAVALDKQVKISAEMEQVLQ
QNDRPAIRIINDIHLDKVTSFVAPQVIPEIGSVILLRMISDNTHLGVVGFYAKQAGQFKPLHADLIEPHKDTFSLITAFN
LRGRNLLKANQVLSQENDNLKRAMFSPDGVVGADGGLKKVMVQVDAIAALNTSVLMLGETGCGKEVIANAIHAKSNRADK
PFIKVNCGAIPETLLDSELFGYEKGAFTGAETRKAGYFEQANGGTIFLDEIGELPLSAQVRLLRVLQNSSITRVGGHENV
DLNIRVIAATHRNLQAMVHEKTFREDLWYRLSVFPIEIPALRQRRSDIPLLVQYFIETLAAKFNLEQLPRVMPEQLAMLN
NYNWPGNVRELINVLERAIIQKPMGPLSFDLLQAQPDENTTIKTGQTIVVDPSYASDKLVPLDTMIGKYISHAMRITGGK
LYGPAGAAELLDINPNTLKSKMKKLGLC

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=353, Percent_Identity=42.7762039660057, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1789087, Length=519, Percent_Identity=33.3333333333333, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1788550, Length=358, Percent_Identity=41.340782122905, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1790437, Length=323, Percent_Identity=40.8668730650155, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1790299, Length=341, Percent_Identity=39.0029325513196, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1788905, Length=289, Percent_Identity=40.8304498269896, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=38.9570552147239, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1789233, Length=390, Percent_Identity=37.1794871794872, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=45.9227467811159, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1786524, Length=252, Percent_Identity=44.4444444444444, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1787583, Length=235, Percent_Identity=41.2765957446808, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI87081858, Length=296, Percent_Identity=32.4324324324324, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789828, Length=280, Percent_Identity=30.3571428571429, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56627; Mature: 56627

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEQFYYQATQCLCSSLSLEVALQNYYEYVKQYLPIDGIFLNIYRPEHADIQFLAHVNEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCH
QAVALDKQVKISAEMEQVLQQNDRPAIRIINDIHLDKVTSFVAPQVIPEIGSVILLRMIS
HHHHHCCCEEHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DNTHLGVVGFYAKQAGQFKPLHADLIEPHKDTFSLITAFNLRGRNLLKANQVLSQENDNL
CCCCEEEEEEEHHCCCCCCCCHHHHCCCCCHHHHHHHEECCCCCCHHHHHHHHHCCCCCH
KRAMFSPDGVVGADGGLKKVMVQVDAIAALNTSVLMLGETGCGKEVIANAIHAKSNRADK
HHHHCCCCCCEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCC
PFIKVNCGAIPETLLDSELFGYEKGAFTGAETRKAGYFEQANGGTIFLDEIGELPLSAQV
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHH
RLLRVLQNSSITRVGGHENVDLNIRVIAATHRNLQAMVHEKTFREDLWYRLSVFPIEIPA
HHHHHHHCCCEEECCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCEEEEEEEEECHH
LRQRRSDIPLLVQYFIETLAAKFNLEQLPRVMPEQLAMLNNYNWPGNVRELINVLERAII
HHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QKPMGPLSFDLLQAQPDENTTIKTGQTIVVDPSYASDKLVPLDTMIGKYISHAMRITGGK
HCCCCCCCHHHHCCCCCCCCEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCE
LYGPAGAAELLDINPNTLKSKMKKLGLC
EECCCCCCEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEQFYYQATQCLCSSLSLEVALQNYYEYVKQYLPIDGIFLNIYRPEHADIQFLAHVNEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCH
QAVALDKQVKISAEMEQVLQQNDRPAIRIINDIHLDKVTSFVAPQVIPEIGSVILLRMIS
HHHHHCCCEEHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DNTHLGVVGFYAKQAGQFKPLHADLIEPHKDTFSLITAFNLRGRNLLKANQVLSQENDNL
CCCCEEEEEEEHHCCCCCCCCHHHHCCCCCHHHHHHHEECCCCCCHHHHHHHHHCCCCCH
KRAMFSPDGVVGADGGLKKVMVQVDAIAALNTSVLMLGETGCGKEVIANAIHAKSNRADK
HHHHCCCCCCEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCC
PFIKVNCGAIPETLLDSELFGYEKGAFTGAETRKAGYFEQANGGTIFLDEIGELPLSAQV
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHH
RLLRVLQNSSITRVGGHENVDLNIRVIAATHRNLQAMVHEKTFREDLWYRLSVFPIEIPA
HHHHHHHCCCEEECCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCEEEEEEEEECHH
LRQRRSDIPLLVQYFIETLAAKFNLEQLPRVMPEQLAMLNNYNWPGNVRELINVLERAII
HHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QKPMGPLSFDLLQAQPDENTTIKTGQTIVVDPSYASDKLVPLDTMIGKYISHAMRITGGK
HCCCCCCCHHHHCCCCCCCCEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCE
LYGPAGAAELLDINPNTLKSKMKKLGLC
EECCCCCCEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]