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The map label for this gene is rsuA [H]

Identifier: 167625202

GI number: 167625202

Start: 4012152

End: 4012868

Strand: Reverse

Name: rsuA [H]

Synonym: Shal_3290

Alternate gene names: 167625202

Gene position: 4012868-4012152 (Counterclockwise)

Preceding gene: 167625205

Following gene: 167625199

Centisome position: 76.77

GC content: 48.54

Gene sequence:

>717_bases
ATGCGTTTAGACAAATTTATCTGCAAAAGCACCGAGCTTAATCGTGCTCAGGCTAGCGAGTGTATTGAGTGCGCAGAAAT
TAGCGTTAATGGTGAGACGATAACGGAAGTGAGTTTTCAGGTACATGAAAGCAATAGCGTTGTCTTCAAAGGTCAAAGGT
TAGTGGCGCGGCCATCACGTTACATCATGCTGCATAAGCCGATACACACTCTGAGTTCCAATGTTGATGGCGACTACCCA
AGCCTGTTTCGCTGTATCAATATTGAAAGAGCGGAAGAACTGCATATTGTTGGGCGGCTCGATGCCGATACCACGGGCTT
AGTGTTGTTGACCGACGATGGCCGCTGGTCATTTGATATTATCAGGCCCGATAAGCAGTGCGAGAAAACCTACCGGGTGA
CATTAAGAGACGCGATTCGTGATGATGTAGCAGATATGGTTGCTAGGTTTGCACATGGGCTGCAATTGCAAAATGAGCCC
GCATTAACCCTGCCAGCTAAGCTGGAAGTTGTCACTGAAAAAGAGGTGCTACTCACCATTACCGAGGGAAAATACCATCA
GGTGAAGCGGATGTTTGCCGCGGTGGGCAATCGGGTCAACGGTTTGCACCGAGAGCGAATTGGTGGATTGAGTTTAGAGC
TTGATGTTGGCTGCTGGCGTCATCTAAGTGAGGATGAGGTGAATCGGCTATCGACTCCGACGCCACAATCACCATAA

Upstream 100 bases:

>100_bases
GATAAATACGGGGATTTTTAAATGATCGCTTGTGCAATTTTGGCTGCTGGTGATAATTGTCGTTATCATGAGATAACTCG
ACTCACAAGAATAGAGCCAT

Downstream 100 bases:

>100_bases
TCTAAAGCTTAAAATAAAAAGCCCTTAACGCTATATAGGTTAAGGGCTTTTTAATGGAGGCTTCTCTCAAGCTTTAGTCA
ACCCAGTGCAATACATCACG

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRLDKFICKSTELNRAQASECIECAEISVNGETITEVSFQVHESNSVVFKGQRLVARPSRYIMLHKPIHTLSSNVDGDYP
SLFRCINIERAEELHIVGRLDADTTGLVLLTDDGRWSFDIIRPDKQCEKTYRVTLRDAIRDDVADMVARFAHGLQLQNEP
ALTLPAKLEVVTEKEVLLTITEGKYHQVKRMFAAVGNRVNGLHRERIGGLSLELDVGCWRHLSEDEVNRLSTPTPQSP

Sequences:

>Translated_238_residues
MRLDKFICKSTELNRAQASECIECAEISVNGETITEVSFQVHESNSVVFKGQRLVARPSRYIMLHKPIHTLSSNVDGDYP
SLFRCINIERAEELHIVGRLDADTTGLVLLTDDGRWSFDIIRPDKQCEKTYRVTLRDAIRDDVADMVARFAHGLQLQNEP
ALTLPAKLEVVTEKEVLLTITEGKYHQVKRMFAAVGNRVNGLHRERIGGLSLELDVGCWRHLSEDEVNRLSTPTPQSP
>Mature_238_residues
MRLDKFICKSTELNRAQASECIECAEISVNGETITEVSFQVHESNSVVFKGQRLVARPSRYIMLHKPIHTLSSNVDGDYP
SLFRCINIERAEELHIVGRLDADTTGLVLLTDDGRWSFDIIRPDKQCEKTYRVTLRDAIRDDVADMVARFAHGLQLQNEP
ALTLPAKLEVVTEKEVLLTITEGKYHQVKRMFAAVGNRVNGLHRERIGGLSLELDVGCWRHLSEDEVNRLSTPTPQSP

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=48.2608695652174, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=29.4871794871795, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787524, Length=241, Percent_Identity=27.3858921161826, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081838, Length=217, Percent_Identity=28.110599078341, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26910; Mature: 26910

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFICKSTELNRAQASECIECAEISVNGETITEVSFQVHESNSVVFKGQRLVARPSR
CCCHHHHHCCCCCCHHHHHHHHHHHEEEECCCEEEEEEEEEECCCCEEEECCEEEECCCE
YIMLHKPIHTLSSNVDGDYPSLFRCINIERAEELHIVGRLDADTTGLVLLTDDGRWSFDI
EEEEECCHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCEEEEE
IRPDKQCEKTYRVTLRDAIRDDVADMVARFAHGLQLQNEPALTLPAKLEVVTEKEVLLTI
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEECCEEEEEE
TEGKYHQVKRMFAAVGNRVNGLHRERIGGLSLELDVGCWRHLSEDEVNRLSTPTPQSP
ECCHHHHHHHHHHHHCHHHCCHHHHHCCCEEEEEECHHHHCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRLDKFICKSTELNRAQASECIECAEISVNGETITEVSFQVHESNSVVFKGQRLVARPSR
CCCHHHHHCCCCCCHHHHHHHHHHHEEEECCCEEEEEEEEEECCCCEEEECCEEEECCCE
YIMLHKPIHTLSSNVDGDYPSLFRCINIERAEELHIVGRLDADTTGLVLLTDDGRWSFDI
EEEEECCHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCEEEEE
IRPDKQCEKTYRVTLRDAIRDDVADMVARFAHGLQLQNEPALTLPAKLEVVTEKEVLLTI
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEECCEEEEEE
TEGKYHQVKRMFAAVGNRVNGLHRERIGGLSLELDVGCWRHLSEDEVNRLSTPTPQSP
ECCHHHHHHHHHHHHCHHHCCHHHHHCCCEEEEEECHHHHCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]