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The map label for this gene is phsA [H]

Identifier: 167625039

GI number: 167625039

Start: 3814497

End: 3816773

Strand: Reverse

Name: phsA [H]

Synonym: Shal_3127

Alternate gene names: 167625039

Gene position: 3816773-3814497 (Counterclockwise)

Preceding gene: 167625040

Following gene: 167625038

Centisome position: 73.02

GC content: 48.48

Gene sequence:

>2277_bases
ATGAAACTAGATCGGCGAACCTTTATCAAAGGGGCCGGAGCCGGCACAGCGACTTGCGCATTAGCAACGCTTCCAGGCTC
TTTAGCTGCCTTTGGGGATTCAAAACTGCAAGGTAGTGCAGAGTCTGTCGCAAGTATTTGTGAAATGTGTTCAACCCGCT
GCCCAATATCGGCACGCGTCGTCAATGGTAAGAACGTATCGATTCTTGGCAACAAGAAGGCCGAGGATTTTGGCGGCGCC
GTCTGTGCCCGCGGTGGTGCGGGTCACAGCCAGCTTTATGATAAACAGCGTATCGTTAAGCCATTGAAGCGCGTTGGTGA
GCGTGGTGAAGGCAACTGGCAAGAGATCGAGTGGGATGAAGCCTATGCGCTAATCGCACAGAACCTTAATAAGATTAAAG
CTGAGCATGGCGCAGAGTCTATCGCTTTCTCATCAAAATCAGGTTCACTATCCGGTCACCTTTTCCACTTGGCTAAATCT
TTCGGCAGCCCTAATACCTTCACTCACGCCTCTACCTGTCCAGGTGCCTATGTTGTCGCTGCCAAGGCGATGTTTGGTGG
CAAGCTCAAGCGTGACATAAGCCACTCTAAGTACATCATCAACTTCGGTCATAACCTCTATGAAGGTATCAATATGGCTG
AGACCAGAGGCATGATGGATGCACAGATGGAGAAGGGCGCAAAGCTAGTGGTATTTGAACCACGCTTCTCGATTGTGGCT
GACAAAGCCGATGAATGGTATGCGATTAAACCAGGTACTGATACCGCAGTTGCATTAGCGATATGTCATGTTTTGATTGA
AGATGACTTATACGATAAAGCGTTCGTCGCGCAGTACGTGTCAGGCTTCGATGAGTTCGCTAAAGAAGTTAAAGCCTACA
CACCTGAGTGGGCTGAATCTATCAGTGACGTACCCGCTGAAGATATTCGCCGCATTACTCATGAATATGCTGCCGCTGCT
CCCCATGCGATGGTGGATTTTGGTCACCGTTCAACGTTTACCACCGAAGAGTTTGAGATGCGCCGCGCGCTATACGCTGC
CAACGTATTGATTGGTAACATTGAGCGTAAAGGTGGCTTGTATTTTGGTAAGAAAGCTGCGAGTTACAACAAGTTTGCTG
GTGAAACAGTCGCACCTACCTTAGCTAAGCCTGGTGTCGATGGTATGCCGAAAATTGATGCTAAGCGTATCGATATGGTG
GATGAACAGTATGCCATGATGTGGTCCTCTGGCGGTATTTACCAGACAATTCTAGATGCAACACTTGAAGCTGTACCATA
TCAGTTACGCGCCTGGGTGATGAGCCGTACCAACCCAATGCAAACCATGACAGATAGAGCGCGTATCGTTAAGACATTGC
AAAAACTCGATTTTGTGGTGGCTTGTGATATTTATATTAGTGAGAGCGCGGCCTATGCCGACATCTTCCTACCTGAGTCG
ACCTATCTTGAGCGTGATGAAGAGATTGCCGACCTGTCGACGAAGAACCCAGCTTACTATGTGCGTCAACGCGTCGTTGA
AACGATTGGTGACACTAAGCCGAGCTGGTTAATCTGGAAAGAGCTTGCCACTAAGTTAGGTCAAGGTCAGTTTTTCCCTT
GGGAGAACATGGAAACTTTGCAGCTGTTCCAAGTGCAGCGTGATTTTGACTTACTAAGCAAGATCAAACAAGACGGTTAC
GTGAGCTATGGTAAGCCGCTGTTGCTGCGTGAGCGCGGCATGGTAAGAGACTTTGTTAATACCTATCCGAATGCTAAGGC
TGTAGACGAAGACGGTACTTACGCGAGCCAGTTAAGCTTTAAGACACCAACGGGCAAGATTGAGTTGAGCTCTGCCAAAG
TCGAGAAGATGGCGCCGGGACGTGGAGTCATTCGTTACCGTGAAGTCACGATGAAAAAGGCCGATGAGCTATTTTTCATT
CAAGGCAAGGTCGCGGTACATACCAACGCAGGTACCCAGAATGTGCCTATGCTGGCCAACCTGATGTCAGATAATGCCGT
GTGGATCCACCCAATCACCGCGGATCTGTTAAAGATCTCAAATGGTGACAAGATTAAGATCTACAACGATACCGGCAGTG
AAGAGGGTCATGCCTTAGTTACCCCTGGTATTCGTCCTGATACTGTCTTTGCCTATATGGGCTGTGGATCAAAGAACAAG
GAACTTGCTCGTGCAACCGGTAAAGGTGTGCATTGCGGTAACTTACTTCCTCATGTGACCTCACCGGTAACGGGTATGAA
CTTGCATACTACCGGTGTCAAGATTGCCAAGATTTAA

Upstream 100 bases:

>100_bases
CAATGATGAAAACCTTAGGAAGAAGTTAATTGTGTTAAACAGTTAACTAGGGGCTTTATCTAACGCTCTCCTAAGGACAA
CTGTCATTGGGAGATTTAAC

Downstream 100 bases:

>100_bases
TCGGAGTTAATTATGACTAAGCGTTATGTTCTCGTGCACGATGAAAACAAGTGCATTGGCTGCCAGGCGTGTAACGTGGC
TTGCCGCAGTGAAAACAATG

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 758; Mature: 758

Protein sequence:

>758_residues
MKLDRRTFIKGAGAGTATCALATLPGSLAAFGDSKLQGSAESVASICEMCSTRCPISARVVNGKNVSILGNKKAEDFGGA
VCARGGAGHSQLYDKQRIVKPLKRVGERGEGNWQEIEWDEAYALIAQNLNKIKAEHGAESIAFSSKSGSLSGHLFHLAKS
FGSPNTFTHASTCPGAYVVAAKAMFGGKLKRDISHSKYIINFGHNLYEGINMAETRGMMDAQMEKGAKLVVFEPRFSIVA
DKADEWYAIKPGTDTAVALAICHVLIEDDLYDKAFVAQYVSGFDEFAKEVKAYTPEWAESISDVPAEDIRRITHEYAAAA
PHAMVDFGHRSTFTTEEFEMRRALYAANVLIGNIERKGGLYFGKKAASYNKFAGETVAPTLAKPGVDGMPKIDAKRIDMV
DEQYAMMWSSGGIYQTILDATLEAVPYQLRAWVMSRTNPMQTMTDRARIVKTLQKLDFVVACDIYISESAAYADIFLPES
TYLERDEEIADLSTKNPAYYVRQRVVETIGDTKPSWLIWKELATKLGQGQFFPWENMETLQLFQVQRDFDLLSKIKQDGY
VSYGKPLLLRERGMVRDFVNTYPNAKAVDEDGTYASQLSFKTPTGKIELSSAKVEKMAPGRGVIRYREVTMKKADELFFI
QGKVAVHTNAGTQNVPMLANLMSDNAVWIHPITADLLKISNGDKIKIYNDTGSEEGHALVTPGIRPDTVFAYMGCGSKNK
ELARATGKGVHCGNLLPHVTSPVTGMNLHTTGVKIAKI

Sequences:

>Translated_758_residues
MKLDRRTFIKGAGAGTATCALATLPGSLAAFGDSKLQGSAESVASICEMCSTRCPISARVVNGKNVSILGNKKAEDFGGA
VCARGGAGHSQLYDKQRIVKPLKRVGERGEGNWQEIEWDEAYALIAQNLNKIKAEHGAESIAFSSKSGSLSGHLFHLAKS
FGSPNTFTHASTCPGAYVVAAKAMFGGKLKRDISHSKYIINFGHNLYEGINMAETRGMMDAQMEKGAKLVVFEPRFSIVA
DKADEWYAIKPGTDTAVALAICHVLIEDDLYDKAFVAQYVSGFDEFAKEVKAYTPEWAESISDVPAEDIRRITHEYAAAA
PHAMVDFGHRSTFTTEEFEMRRALYAANVLIGNIERKGGLYFGKKAASYNKFAGETVAPTLAKPGVDGMPKIDAKRIDMV
DEQYAMMWSSGGIYQTILDATLEAVPYQLRAWVMSRTNPMQTMTDRARIVKTLQKLDFVVACDIYISESAAYADIFLPES
TYLERDEEIADLSTKNPAYYVRQRVVETIGDTKPSWLIWKELATKLGQGQFFPWENMETLQLFQVQRDFDLLSKIKQDGY
VSYGKPLLLRERGMVRDFVNTYPNAKAVDEDGTYASQLSFKTPTGKIELSSAKVEKMAPGRGVIRYREVTMKKADELFFI
QGKVAVHTNAGTQNVPMLANLMSDNAVWIHPITADLLKISNGDKIKIYNDTGSEEGHALVTPGIRPDTVFAYMGCGSKNK
ELARATGKGVHCGNLLPHVTSPVTGMNLHTTGVKIAKI
>Mature_758_residues
MKLDRRTFIKGAGAGTATCALATLPGSLAAFGDSKLQGSAESVASICEMCSTRCPISARVVNGKNVSILGNKKAEDFGGA
VCARGGAGHSQLYDKQRIVKPLKRVGERGEGNWQEIEWDEAYALIAQNLNKIKAEHGAESIAFSSKSGSLSGHLFHLAKS
FGSPNTFTHASTCPGAYVVAAKAMFGGKLKRDISHSKYIINFGHNLYEGINMAETRGMMDAQMEKGAKLVVFEPRFSIVA
DKADEWYAIKPGTDTAVALAICHVLIEDDLYDKAFVAQYVSGFDEFAKEVKAYTPEWAESISDVPAEDIRRITHEYAAAA
PHAMVDFGHRSTFTTEEFEMRRALYAANVLIGNIERKGGLYFGKKAASYNKFAGETVAPTLAKPGVDGMPKIDAKRIDMV
DEQYAMMWSSGGIYQTILDATLEAVPYQLRAWVMSRTNPMQTMTDRARIVKTLQKLDFVVACDIYISESAAYADIFLPES
TYLERDEEIADLSTKNPAYYVRQRVVETIGDTKPSWLIWKELATKLGQGQFFPWENMETLQLFQVQRDFDLLSKIKQDGY
VSYGKPLLLRERGMVRDFVNTYPNAKAVDEDGTYASQLSFKTPTGKIELSSAKVEKMAPGRGVIRYREVTMKKADELFFI
QGKVAVHTNAGTQNVPMLANLMSDNAVWIHPITADLLKISNGDKIKIYNDTGSEEGHALVTPGIRPDTVFAYMGCGSKNK
ELARATGKGVHCGNLLPHVTSPVTGMNLHTTGVKIAKI

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=847, Percent_Identity=27.0365997638725, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1787870, Length=847, Percent_Identity=26.2101534828808, Blast_Score=189, Evalue=6e-49,
Organism=Escherichia coli, GI171474008, Length=852, Percent_Identity=24.7652582159624, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI3868721, Length=588, Percent_Identity=26.530612244898, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI145693196, Length=671, Percent_Identity=22.8017883755589, Blast_Score=112, Evalue=9e-26,
Organism=Escherichia coli, GI1787231, Length=709, Percent_Identity=21.015514809591, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI87081994, Length=784, Percent_Identity=22.1938775510204, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI3868720, Length=311, Percent_Identity=25.4019292604502, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1788534, Length=547, Percent_Identity=21.0237659963437, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 83321; Mature: 83321

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS00464 RIBOSOMAL_L22 ; PS00551 MOLYBDOPTERIN_PROK_1 ; PS00729 AP_NUCLEASE_F2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDRRTFIKGAGAGTATCALATLPGSLAAFGDSKLQGSAESVASICEMCSTRCPISARV
CCCCCCHHEECCCCCHHEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
VNGKNVSILGNKKAEDFGGAVCARGGAGHSQLYDKQRIVKPLKRVGERGEGNWQEIEWDE
ECCCEEEEEECCCCCHHCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECHHH
AYALIAQNLNKIKAEHGAESIAFSSKSGSLSGHLFHLAKSFGSPNTFTHASTCPGAYVVA
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
AKAMFGGKLKRDISHSKYIINFGHNLYEGINMAETRGMMDAQMEKGAKLVVFEPRFSIVA
HHHHHCCHHHHCCCCCEEEEECCCHHHHCCCHHHHCCCHHHHHHCCCEEEEECCCCEEEE
DKADEWYAIKPGTDTAVALAICHVLIEDDLYDKAFVAQYVSGFDEFAKEVKAYTPEWAES
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ISDVPAEDIRRITHEYAAAAPHAMVDFGHRSTFTTEEFEMRRALYAANVLIGNIERKGGL
HHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCE
YFGKKAASYNKFAGETVAPTLAKPGVDGMPKIDAKRIDMVDEQYAMMWSSGGIYQTILDA
EECCCCCCCCHHCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
TLEAVPYQLRAWVMSRTNPMQTMTDRARIVKTLQKLDFVVACDIYISESAAYADIFLPES
HHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEEECCCC
TYLERDEEIADLSTKNPAYYVRQRVVETIGDTKPSWLIWKELATKLGQGQFFPWENMETL
HHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCCCCCCHH
QLFQVQRDFDLLSKIKQDGYVSYGKPLLLRERGMVRDFVNTYPNAKAVDEDGTYASQLSF
HHHHHHHHHHHHHHHHHCCCEECCCCEEECCCCHHHHHHHHCCCCCCCCCCCCCHHEEEE
KTPTGKIELSSAKVEKMAPGRGVIRYREVTMKKADELFFIQGKVAVHTNAGTQNVPMLAN
CCCCCEEEECCCHHHHCCCCCCEEEEHHHHHHCCCCEEEEEEEEEEEECCCCCCCCHHHH
LMSDNAVWIHPITADLLKISNGDKIKIYNDTGSEEGHALVTPGIRPDTVFAYMGCGSKNK
HHCCCCEEEEECHHHHEEECCCCEEEEECCCCCCCCCEEECCCCCCCCEEEEECCCCCCC
ELARATGKGVHCGNLLPHVTSPVTGMNLHTTGVKIAKI
HHHHHCCCCCCCCCCCCHHCCCCCCCEEEECCEEEEEC
>Mature Secondary Structure
MKLDRRTFIKGAGAGTATCALATLPGSLAAFGDSKLQGSAESVASICEMCSTRCPISARV
CCCCCCHHEECCCCCHHEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
VNGKNVSILGNKKAEDFGGAVCARGGAGHSQLYDKQRIVKPLKRVGERGEGNWQEIEWDE
ECCCEEEEEECCCCCHHCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECHHH
AYALIAQNLNKIKAEHGAESIAFSSKSGSLSGHLFHLAKSFGSPNTFTHASTCPGAYVVA
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
AKAMFGGKLKRDISHSKYIINFGHNLYEGINMAETRGMMDAQMEKGAKLVVFEPRFSIVA
HHHHHCCHHHHCCCCCEEEEECCCHHHHCCCHHHHCCCHHHHHHCCCEEEEECCCCEEEE
DKADEWYAIKPGTDTAVALAICHVLIEDDLYDKAFVAQYVSGFDEFAKEVKAYTPEWAES
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ISDVPAEDIRRITHEYAAAAPHAMVDFGHRSTFTTEEFEMRRALYAANVLIGNIERKGGL
HHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCE
YFGKKAASYNKFAGETVAPTLAKPGVDGMPKIDAKRIDMVDEQYAMMWSSGGIYQTILDA
EECCCCCCCCHHCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
TLEAVPYQLRAWVMSRTNPMQTMTDRARIVKTLQKLDFVVACDIYISESAAYADIFLPES
HHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEEECCCC
TYLERDEEIADLSTKNPAYYVRQRVVETIGDTKPSWLIWKELATKLGQGQFFPWENMETL
HHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCCCCCCHH
QLFQVQRDFDLLSKIKQDGYVSYGKPLLLRERGMVRDFVNTYPNAKAVDEDGTYASQLSF
HHHHHHHHHHHHHHHHHCCCEECCCCEEECCCCHHHHHHHHCCCCCCCCCCCCCHHEEEE
KTPTGKIELSSAKVEKMAPGRGVIRYREVTMKKADELFFIQGKVAVHTNAGTQNVPMLAN
CCCCCEEEECCCHHHHCCCCCCEEEEHHHHHHCCCCEEEEEEEEEEEECCCCCCCCHHHH
LMSDNAVWIHPITADLLKISNGDKIKIYNDTGSEEGHALVTPGIRPDTVFAYMGCGSKNK
HHCCCCEEEEECHHHHEEECCCCEEEEECCCCCCCCCEEECCCCCCCCEEEEECCCCCCC
ELARATGKGVHCGNLLPHVTSPVTGMNLHTTGVKIAKI
HHHHHCCCCCCCCCCCCHHCCCCCCCEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]