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The map label for this gene is gcvA [H]

Identifier: 167624986

GI number: 167624986

Start: 3749686

End: 3750597

Strand: Reverse

Name: gcvA [H]

Synonym: Shal_3073

Alternate gene names: 167624986

Gene position: 3750597-3749686 (Counterclockwise)

Preceding gene: 167624988

Following gene: 167624985

Centisome position: 71.76

GC content: 45.83

Gene sequence:

>912_bases
ATGGCAAGACGATTACCTCCCCTAAATGCTGTAAAGGCATTTGAGGCTGCAGCAAGGCATTTAAGCTTCACTCGAGCAGC
TGAAGAGTTGTTTGTTACTCAGGCTGCAGTGAGTCATCAAATTAAAGCGTTAGAAGAGTATCTTGGATTAAAATTATTCC
GTCGCAAGAACCGTTCATTGCTGTTAACCGAGGAGGGGCAAAGCTACTTTCTCGATATCAAAGATATTTTCACTCAGCTT
GCAGATGCGACCGATAGGTTATTAGCAAGAAGCGCCATCGGCTCGCTTACAGTGGCGACTTCGCCTAGCTTTGCTATTCA
ATGGCTCGTACCGAGACTCGCCAAATTTAGCGAGAAAAATCCAGAGATCGATGTCAGAATTAAAGCGGTTGATGACGAGG
ATAGCTCGCTCACCGATGATGTCGATGTCGCCATCTATTATGGCATGGGTAACTGGCCTGGAATGCGTGCCGATAAGTTA
AGAAATGAAGTATTAATTCCGGTCTGTTCACCTTTATTACTCAATGGTCCTAAGCCGTTAGAGAAGCCGAGCGATCTTAA
AAATCACACCTTATTACATGACTCTAGCCGCCAAGATTGGTTGGCTTGGTTTAGGCAGTGTGGAATTACTGATATTAATG
TGAACCAGGGGCCAATATTTAGTCATTCTTCACTTGTGTTGCAAGCGGCCGCCCACGGTCAAGGTGTGGCGCTAGGCTAC
AGTGTGCTTGCAAGGCCCGACATTAAAGCAGGACGATTGGTGTGTCCATTCCCTGAAGTTTTAGTCAGTAAGAATGCATA
TTATCTAGTGTGCCAGCAAAACCATGCCGAGATAGGTAAGGTTGCGGCCTTTAGAGAGTGGATGCTTGACATGTTTGAAG
AGGAGACCCGTAGTGAGCTGCTTACAGGATAA

Upstream 100 bases:

>100_bases
CGTTGGGGCTGATTTTGAACAAAAAGACTGGCCTTTACAAACGATAAAATGTAACAATGCTCATTAGAAAAACTAATTAA
AAATCGGGATCTGGATCACT

Downstream 100 bases:

>100_bases
GTTAAAGCAAGCGTATTTAGCGGGGGAATTAGCAGAGGCTGTTGCAGGCCTAGGTCTCGAGGACTTAGTCTTCGATGCCA
GCCAGTATTTGGTTGAGGAT

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MARRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEYLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQL
ADATDRLLARSAIGSLTVATSPSFAIQWLVPRLAKFSEKNPEIDVRIKAVDDEDSSLTDDVDVAIYYGMGNWPGMRADKL
RNEVLIPVCSPLLLNGPKPLEKPSDLKNHTLLHDSSRQDWLAWFRQCGITDINVNQGPIFSHSSLVLQAAAHGQGVALGY
SVLARPDIKAGRLVCPFPEVLVSKNAYYLVCQQNHAEIGKVAAFREWMLDMFEEETRSELLTG

Sequences:

>Translated_303_residues
MARRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEYLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQL
ADATDRLLARSAIGSLTVATSPSFAIQWLVPRLAKFSEKNPEIDVRIKAVDDEDSSLTDDVDVAIYYGMGNWPGMRADKL
RNEVLIPVCSPLLLNGPKPLEKPSDLKNHTLLHDSSRQDWLAWFRQCGITDINVNQGPIFSHSSLVLQAAAHGQGVALGY
SVLARPDIKAGRLVCPFPEVLVSKNAYYLVCQQNHAEIGKVAAFREWMLDMFEEETRSELLTG
>Mature_302_residues
ARRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEYLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQLA
DATDRLLARSAIGSLTVATSPSFAIQWLVPRLAKFSEKNPEIDVRIKAVDDEDSSLTDDVDVAIYYGMGNWPGMRADKLR
NEVLIPVCSPLLLNGPKPLEKPSDLKNHTLLHDSSRQDWLAWFRQCGITDINVNQGPIFSHSSLVLQAAAHGQGVALGYS
VLARPDIKAGRLVCPFPEVLVSKNAYYLVCQQNHAEIGKVAAFREWMLDMFEEETRSELLTG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=70.242214532872, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=36.5853658536585, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=31.5972222222222, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=26.2458471760797, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786401, Length=173, Percent_Identity=31.7919075144509, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI157672245, Length=185, Percent_Identity=31.8918918918919, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081978, Length=271, Percent_Identity=29.1512915129151, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789639, Length=194, Percent_Identity=26.8041237113402, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=31.5384615384615, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787128, Length=131, Percent_Identity=32.0610687022901, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=134, Percent_Identity=34.3283582089552, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082132, Length=156, Percent_Identity=26.2820512820513, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=244, Percent_Identity=27.8688524590164, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693105, Length=169, Percent_Identity=29.585798816568, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789440, Length=260, Percent_Identity=26.1538461538462, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33850; Mature: 33719

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEYLGLKLFRRKNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLTEEGQSYFLDIKDIFTQLADATDRLLARSAIGSLTVATSPSFAIQWLVPRLAKFSEKN
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCC
PEIDVRIKAVDDEDSSLTDDVDVAIYYGMGNWPGMRADKLRNEVLIPVCSPLLLNGPKPL
CCEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHCCEEEEECCHHHCCCCCCC
EKPSDLKNHTLLHDSSRQDWLAWFRQCGITDINVNQGPIFSHSSLVLQAAAHGQGVALGY
CCCCCCCCCEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHEEHHCCCCCEEEHH
SVLARPDIKAGRLVCPFPEVLVSKNAYYLVCQQNHAEIGKVAAFREWMLDMFEEETRSEL
HHHCCCCCCCCCEECCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTG
CCC
>Mature Secondary Structure 
ARRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEYLGLKLFRRKNRSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLTEEGQSYFLDIKDIFTQLADATDRLLARSAIGSLTVATSPSFAIQWLVPRLAKFSEKN
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCC
PEIDVRIKAVDDEDSSLTDDVDVAIYYGMGNWPGMRADKLRNEVLIPVCSPLLLNGPKPL
CCEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHCCEEEEECCHHHCCCCCCC
EKPSDLKNHTLLHDSSRQDWLAWFRQCGITDINVNQGPIFSHSSLVLQAAAHGQGVALGY
CCCCCCCCCEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHEEHHCCCCCEEEHH
SVLARPDIKAGRLVCPFPEVLVSKNAYYLVCQQNHAEIGKVAAFREWMLDMFEEETRSEL
HHHCCCCCCCCCEECCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]