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The map label for this gene is phoB [H]

Identifier: 167624972

GI number: 167624972

Start: 3730537

End: 3731226

Strand: Reverse

Name: phoB [H]

Synonym: Shal_3059

Alternate gene names: 167624972

Gene position: 3731226-3730537 (Counterclockwise)

Preceding gene: 167624973

Following gene: 167624971

Centisome position: 71.38

GC content: 47.54

Gene sequence:

>690_bases
ATGACTGCAAGGATTTTGATAGTTGAAGACGAGTTAGCCATCCGCGAGATGTTAACTTTTGTTTTAGAACAACACGGCTT
TACCACAGCAGCTGCAGAGGACTATGACTCGGCATTAGCGATGTTAACGGAGCCATACCCTGATCTTGTCTTGCTTGATT
GGATGTTCCCAGGTGGTAATGGCATCCAGTTAGCCAAGCGTCTGCGCCAAGATGAATTCACACGTCATATTCCAATTATC
ATGTTGACGGCACGTGGTGAGGAGGAAGATAAGGTTAGAGGGCTAGAGGTTGGCGCCGATGACTTTATGACCAAACCATT
TTCACCCAAAGAGTTGGTCGCTCGCATTAAGGCTGTAATGCGTCGTAGTGCGCCTACATGCTTAGAGGAGATCATCGATG
TGCAAGGGTTGCAACTAGACCCTGTCAGTCATCGTGTGACCGTCAATGATCAAGTACTCGATATGGGGCCAACGGAGTTT
AGATTATTACACTTCTTTATGACTCATCAAGAGCGTGTTTATAGCCGTGAGCAACTTCTCGATAATGTGTGGGGAACCAA
TGTTTATGTTGAAGACCGCACTGTCGACGTACACATTCGACGCTTACGAAAGGCGGTGACCCCTTCGGGGCATGATCGCC
TTATCCAAACCGTCCGTGGTGCAGGTTATCGTTTCTCTACCAAGCTTTAG

Upstream 100 bases:

>100_bases
AATGCACATGAATATGGCAAAATATCTTAGTGTTTAATATTACTGTCATAAAAGTGACCAATAATAGCCAACGTAGACAT
TCACTGATAGAAGATCGAAT

Downstream 100 bases:

>100_bases
TTTAATAGGCACAAGGTTACTTTTTACGTTATCTTGTGCCAAGTTCTCCCATATGGTCGTTATATTGGTTAAATATGTTT
GATTCATATTCAGGGTACCG

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTARILIVEDELAIREMLTFVLEQHGFTTAAAEDYDSALAMLTEPYPDLVLLDWMFPGGNGIQLAKRLRQDEFTRHIPII
MLTARGEEEDKVRGLEVGADDFMTKPFSPKELVARIKAVMRRSAPTCLEEIIDVQGLQLDPVSHRVTVNDQVLDMGPTEF
RLLHFFMTHQERVYSREQLLDNVWGTNVYVEDRTVDVHIRRLRKAVTPSGHDRLIQTVRGAGYRFSTKL

Sequences:

>Translated_229_residues
MTARILIVEDELAIREMLTFVLEQHGFTTAAAEDYDSALAMLTEPYPDLVLLDWMFPGGNGIQLAKRLRQDEFTRHIPII
MLTARGEEEDKVRGLEVGADDFMTKPFSPKELVARIKAVMRRSAPTCLEEIIDVQGLQLDPVSHRVTVNDQVLDMGPTEF
RLLHFFMTHQERVYSREQLLDNVWGTNVYVEDRTVDVHIRRLRKAVTPSGHDRLIQTVRGAGYRFSTKL
>Mature_228_residues
TARILIVEDELAIREMLTFVLEQHGFTTAAAEDYDSALAMLTEPYPDLVLLDWMFPGGNGIQLAKRLRQDEFTRHIPIIM
LTARGEEEDKVRGLEVGADDFMTKPFSPKELVARIKAVMRRSAPTCLEEIIDVQGLQLDPVSHRVTVNDQVLDMGPTEFR
LLHFFMTHQERVYSREQLLDNVWGTNVYVEDRTVDVHIRRLRKAVTPSGHDRLIQTVRGAGYRFSTKL

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=72.0524017467249, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=38.4955752212389, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=37.7272727272727, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=36.2445414847162, Blast_Score=146, Evalue=9e-37,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=35.5855855855856, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=40.6113537117904, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1790863, Length=241, Percent_Identity=36.5145228215768, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=35.8407079646018, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=34.9775784753363, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=32.4444444444444, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=34.5454545454545, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1789402, Length=226, Percent_Identity=32.7433628318584, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=31.7391304347826, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=29.4871794871795, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=42.1052631578947, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=27.5, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=32.1739130434783, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26267; Mature: 26135

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARILIVEDELAIREMLTFVLEQHGFTTAAAEDYDSALAMLTEPYPDLVLLDWMFPGGN
CCEEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GIQLAKRLRQDEFTRHIPIIMLTARGEEEDKVRGLEVGADDFMTKPFSPKELVARIKAVM
HHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEECCHHHHCCCCCHHHHHHHHHHHH
RRSAPTCLEEIIDVQGLQLDPVSHRVTVNDQVLDMGPTEFRLLHFFMTHQERVYSREQLL
HCCCCHHHHHHHCCCCCEECCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DNVWGTNVYVEDRTVDVHIRRLRKAVTPSGHDRLIQTVRGAGYRFSTKL
HHHCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCC
>Mature Secondary Structure 
TARILIVEDELAIREMLTFVLEQHGFTTAAAEDYDSALAMLTEPYPDLVLLDWMFPGGN
CEEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GIQLAKRLRQDEFTRHIPIIMLTARGEEEDKVRGLEVGADDFMTKPFSPKELVARIKAVM
HHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEECCHHHHCCCCCHHHHHHHHHHHH
RRSAPTCLEEIIDVQGLQLDPVSHRVTVNDQVLDMGPTEFRLLHFFMTHQERVYSREQLL
HCCCCHHHHHHHCCCCCEECCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DNVWGTNVYVEDRTVDVHIRRLRKAVTPSGHDRLIQTVRGAGYRFSTKL
HHHCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]