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The map label for this gene is purU [H]

Identifier: 167624900

GI number: 167624900

Start: 3631599

End: 3632432

Strand: Reverse

Name: purU [H]

Synonym: Shal_2986

Alternate gene names: 167624900

Gene position: 3632432-3631599 (Counterclockwise)

Preceding gene: 167624901

Following gene: 167624895

Centisome position: 69.49

GC content: 42.33

Gene sequence:

>834_bases
ATGCAACGGAAAGTATTGATAACAGATTGTGCGGATGCTCCAGGGCTGATAACCAAGATCACCCGTGTTTGTTTTGAGCA
TGGGCTTAATATTACAAAAAATAGTGAGTTTGTTGATCATGCTCATGGGCGATTCTTTATGCGTACAGAGCTAGAGGGGA
GCTTCGAGGAAGAGTTATTGCTCAATAAGCTTCAGTCTGTTCTTCCAGAAAAGAATCACACTAAGCTCGTCGATGCCGGT
AAAAAGCGCATTGTGATTATGGTCACTAAAGAAGCACATTGCTTAGGTGATATATTAATTAAATCCTATTCCGGGGCGCT
TAATGTCGAGATTGCCGCTGTTATAGGAAATTACGACACGCTTAAGCCTTTGGTAGAGAAGTTTGATATTCCATTTCACG
GCATTAGCCATCAAGAATTGAGTCGCAGCGAGCATGAAGAAGCAATGCAAAAGGCGATCACAGCTTATGATCCCGATTAT
ATTGTGCTGGCAAAGTATATGCGGATTTTGACGCCTGAATTTGTACGTCAATATCAAAGTAAAATGATTAATATTCATCA
CTCATTCTTACCTGCATTTGTCGGTGCAGCTCCCTATAAGCAAGCATGGGAGCGAGGGGTTAAGATCATAGGGGCAACGG
CGCACTTTGTGACGGATTCGCTAGATGAGGGACCCATTATCAAACAAGACGTGATCCCAGTTGATCATAGCTTTAGCGCT
GAGGAGTTGGTTCGTTGCGGGCGCGATGTTGAAAAAAGCGTATTGAGTAAAGCACTACATTTAGTGATTAATGAAGAGGT
GATTGTTTATGGTAATAAAACGGTGGTGTTTTAG

Upstream 100 bases:

>100_bases
GTGAGTAGATAAAGATATATCGGCTCAGGCTGTTGTAGGTATTTTCTAAGTTGATTAGTTTGGTGTTGATAATGAGTGCA
GACTGAGAGGAAGAGGGTAT

Downstream 100 bases:

>100_bases
CTTATAAGTTTCTGTTTTAAGCAAAATAAAAGAGGCTTTCGCCTCTGTTATTTTTACTGAAGGGGAACCTATTCATAGTT
AACAACATAAAACTCTGCAT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MQRKVLITDCADAPGLITKITRVCFEHGLNITKNSEFVDHAHGRFFMRTELEGSFEEELLLNKLQSVLPEKNHTKLVDAG
KKRIVIMVTKEAHCLGDILIKSYSGALNVEIAAVIGNYDTLKPLVEKFDIPFHGISHQELSRSEHEEAMQKAITAYDPDY
IVLAKYMRILTPEFVRQYQSKMINIHHSFLPAFVGAAPYKQAWERGVKIIGATAHFVTDSLDEGPIIKQDVIPVDHSFSA
EELVRCGRDVEKSVLSKALHLVINEEVIVYGNKTVVF

Sequences:

>Translated_277_residues
MQRKVLITDCADAPGLITKITRVCFEHGLNITKNSEFVDHAHGRFFMRTELEGSFEEELLLNKLQSVLPEKNHTKLVDAG
KKRIVIMVTKEAHCLGDILIKSYSGALNVEIAAVIGNYDTLKPLVEKFDIPFHGISHQELSRSEHEEAMQKAITAYDPDY
IVLAKYMRILTPEFVRQYQSKMINIHHSFLPAFVGAAPYKQAWERGVKIIGATAHFVTDSLDEGPIIKQDVIPVDHSFSA
EELVRCGRDVEKSVLSKALHLVINEEVIVYGNKTVVF
>Mature_277_residues
MQRKVLITDCADAPGLITKITRVCFEHGLNITKNSEFVDHAHGRFFMRTELEGSFEEELLLNKLQSVLPEKNHTKLVDAG
KKRIVIMVTKEAHCLGDILIKSYSGALNVEIAAVIGNYDTLKPLVEKFDIPFHGISHQELSRSEHEEAMQKAITAYDPDY
IVLAKYMRILTPEFVRQYQSKMINIHHSFLPAFVGAAPYKQAWERGVKIIGATAHFVTDSLDEGPIIKQDVIPVDHSFSA
EELVRCGRDVEKSVLSKALHLVINEEVIVYGNKTVVF

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=200, Percent_Identity=30, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI209869995, Length=200, Percent_Identity=30, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI209869993, Length=200, Percent_Identity=30, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=64.2599277978339, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1788846, Length=94, Percent_Identity=44.6808510638298, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=161, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24582400, Length=200, Percent_Identity=27.5, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31227; Mature: 31227

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKVLITDCADAPGLITKITRVCFEHGLNITKNSEFVDHAHGRFFMRTELEGSFEEELL
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCCHHHHHH
LNKLQSVLPEKNHTKLVDAGKKRIVIMVTKEAHCLGDILIKSYSGALNVEIAAVIGNYDT
HHHHHHHCCCCCCCHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCHH
LKPLVEKFDIPFHGISHQELSRSEHEEAMQKAITAYDPDYIVLAKYMRILTPEFVRQYQS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHH
KMINIHHSFLPAFVGAAPYKQAWERGVKIIGATAHFVTDSLDEGPIIKQDVIPVDHSFSA
HHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECHHHHHHCCCCCCCCCHHCCCCCCCCCCH
EELVRCGRDVEKSVLSKALHLVINEEVIVYGNKTVVF
HHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCEEEC
>Mature Secondary Structure
MQRKVLITDCADAPGLITKITRVCFEHGLNITKNSEFVDHAHGRFFMRTELEGSFEEELL
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCCHHHHHH
LNKLQSVLPEKNHTKLVDAGKKRIVIMVTKEAHCLGDILIKSYSGALNVEIAAVIGNYDT
HHHHHHHCCCCCCCHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCHH
LKPLVEKFDIPFHGISHQELSRSEHEEAMQKAITAYDPDYIVLAKYMRILTPEFVRQYQS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHH
KMINIHHSFLPAFVGAAPYKQAWERGVKIIGATAHFVTDSLDEGPIIKQDVIPVDHSFSA
HHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECHHHHHHCCCCCCCCCHHCCCCCCCCCCH
EELVRCGRDVEKSVLSKALHLVINEEVIVYGNKTVVF
HHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]