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The map label for this gene is mexA [H]

Identifier: 167624825

GI number: 167624825

Start: 3539958

End: 3541094

Strand: Reverse

Name: mexA [H]

Synonym: Shal_2911

Alternate gene names: 167624825

Gene position: 3541094-3539958 (Counterclockwise)

Preceding gene: 167624829

Following gene: 167624824

Centisome position: 67.75

GC content: 45.12

Gene sequence:

>1137_bases
ATGGCTAGTGAATTTAAAAAAATTACCCGTTTAGGGCTGTTTGCTGCGGTCTGCTTCTGGACGACTGCCTGTGAGAAAAG
TAGCACTGACGAGGCGTTTAATTATCCAAACACCGCCAATATCGCCACCATAACTGCTGAGTTAACTCAAGTCACCTTGT
TTGATGAATTGCAAGGTCGAGTACGGCCACTACGTAGCGCTGAAATTCGTCCACAGGTGGAAGGAATTATAACCCAGAGG
CTATTTAAGCAAGGAGACACCCTCAAGCAGGGGGATGCACTGTTTCAAATTGACCAAGATATTTTCACTATCGATGTAGA
GATAAAACAAGCGGCGCTTGAGCAATCTATGGCGCACTTAGCGTTAATACAAAGCCAAGTTGATCGCTTTACTCAATTAG
ATCGTACTAAGGCGGTAAGTAAGCAGGCTTTTGAAGAGGTCAGTTTTAACTTACAAATTGCAGCCGCCACAGTGCAGCAA
AATCGAGCCCAACTTAAGCATAGTGAGTTACAACTTGAGTATGCGAAAGTGACTGCACCTATCTCTGGGATTATCGGTGA
GGCATTAGTGACTGAGGGAAGTTTAGTTAGCCGTACCGAGCCACAGCCATTAGCCATCATTCAGCAGATTGATAAGGTCT
ATATTGATGTTCGTCAACCCGCATCGAAACTTGGGTTATTGCGACGTTTATTAGCCAATGAACAGAAAAACAGTGAGCAA
GGGGTCAAGGTGATTGTGCAGTCGTCTGCAGAGAGTGCAGATGATATCGAAGGACAAATTTTGTTTTCAGGGATCAGCGT
TGATGAAAATACCGGTGATCTGATTATTCGTATTATTGCCGATAATGCGCAGCAAAAACTCTTACCTGGAATGTACGTAC
GAACCCAGATCCCGCGTCAGAAATTGCAAGCAATCACGGTTCCTGAACACGCGGTTCGCCGCAACAGTGCGGGCTTACCG
TTTGTCTACATTGTTGTTAACGACAAGTTTGAGCTAAGACCTGTACAACTAGAAGGGCTGCAACAGCGACGATATGTAAT
TTCGAAAGGAGTTAATCCAAACGATATAGTGGTAGTGACTGGCCAAGATAACCTGCGCGAAGGGGCTGAGTTAGTCCTGT
CTGATTGGCAGCAGTAA

Upstream 100 bases:

>100_bases
TGCTCATTTAATCTGTCGCCAACAATTTTTAATGCCTAGGTTTTGTCTTAATTGAGATAGAACATTGGCTGTGCCTGTTA
GGTATGAGTGAAGATAATAT

Downstream 100 bases:

>100_bases
TGTATCAACGGATAGCGTAAGAGGAAGTTTATTGTGTCGTATTTTTTTATTTCTCGACCTGTTTTTGCATGGGTTATTGC
TATTTTAATTAGCATCGCTG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MASEFKKITRLGLFAAVCFWTTACEKSSTDEAFNYPNTANIATITAELTQVTLFDELQGRVRPLRSAEIRPQVEGIITQR
LFKQGDTLKQGDALFQIDQDIFTIDVEIKQAALEQSMAHLALIQSQVDRFTQLDRTKAVSKQAFEEVSFNLQIAAATVQQ
NRAQLKHSELQLEYAKVTAPISGIIGEALVTEGSLVSRTEPQPLAIIQQIDKVYIDVRQPASKLGLLRRLLANEQKNSEQ
GVKVIVQSSAESADDIEGQILFSGISVDENTGDLIIRIIADNAQQKLLPGMYVRTQIPRQKLQAITVPEHAVRRNSAGLP
FVYIVVNDKFELRPVQLEGLQQRRYVISKGVNPNDIVVVTGQDNLREGAELVLSDWQQ

Sequences:

>Translated_378_residues
MASEFKKITRLGLFAAVCFWTTACEKSSTDEAFNYPNTANIATITAELTQVTLFDELQGRVRPLRSAEIRPQVEGIITQR
LFKQGDTLKQGDALFQIDQDIFTIDVEIKQAALEQSMAHLALIQSQVDRFTQLDRTKAVSKQAFEEVSFNLQIAAATVQQ
NRAQLKHSELQLEYAKVTAPISGIIGEALVTEGSLVSRTEPQPLAIIQQIDKVYIDVRQPASKLGLLRRLLANEQKNSEQ
GVKVIVQSSAESADDIEGQILFSGISVDENTGDLIIRIIADNAQQKLLPGMYVRTQIPRQKLQAITVPEHAVRRNSAGLP
FVYIVVNDKFELRPVQLEGLQQRRYVISKGVNPNDIVVVTGQDNLREGAELVLSDWQQ
>Mature_377_residues
ASEFKKITRLGLFAAVCFWTTACEKSSTDEAFNYPNTANIATITAELTQVTLFDELQGRVRPLRSAEIRPQVEGIITQRL
FKQGDTLKQGDALFQIDQDIFTIDVEIKQAALEQSMAHLALIQSQVDRFTQLDRTKAVSKQAFEEVSFNLQIAAATVQQN
RAQLKHSELQLEYAKVTAPISGIIGEALVTEGSLVSRTEPQPLAIIQQIDKVYIDVRQPASKLGLLRRLLANEQKNSEQG
VKVIVQSSAESADDIEGQILFSGISVDENTGDLIIRIIADNAQQKLLPGMYVRTQIPRQKLQAITVPEHAVRRNSAGLPF
VYIVVNDKFELRPVQLEGLQQRRYVISKGVNPNDIVVVTGQDNLREGAELVLSDWQQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=364, Percent_Identity=32.4175824175824, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1789665, Length=322, Percent_Identity=33.8509316770186, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789929, Length=360, Percent_Identity=30.8333333333333, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI87082036, Length=317, Percent_Identity=24.2902208201893, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42095; Mature: 41963

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASEFKKITRLGLFAAVCFWTTACEKSSTDEAFNYPNTANIATITAELTQVTLFDELQGR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHH
VRPLRSAEIRPQVEGIITQRLFKQGDTLKQGDALFQIDQDIFTIDVEIKQAALEQSMAHL
HCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHH
ALIQSQVDRFTQLDRTKAVSKQAFEEVSFNLQIAAATVQQNRAQLKHSELQLEYAKVTAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHEEEHHHHHCC
ISGIIGEALVTEGSLVSRTEPQPLAIIQQIDKVYIDVRQPASKLGLLRRLLANEQKNSEQ
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCC
GVKVIVQSSAESADDIEGQILFSGISVDENTGDLIIRIIADNAQQKLLPGMYVRTQIPRQ
CEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHCCCHHHHHHCCHH
KLQAITVPEHAVRRNSAGLPFVYIVVNDKFELRPVQLEGLQQRRYVISKGVNPNDIVVVT
HHHEECCCHHHHHCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEE
GQDNLREGAELVLSDWQQ
CCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASEFKKITRLGLFAAVCFWTTACEKSSTDEAFNYPNTANIATITAELTQVTLFDELQGR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHH
VRPLRSAEIRPQVEGIITQRLFKQGDTLKQGDALFQIDQDIFTIDVEIKQAALEQSMAHL
HCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHH
ALIQSQVDRFTQLDRTKAVSKQAFEEVSFNLQIAAATVQQNRAQLKHSELQLEYAKVTAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHEEEHHHHHCC
ISGIIGEALVTEGSLVSRTEPQPLAIIQQIDKVYIDVRQPASKLGLLRRLLANEQKNSEQ
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCC
GVKVIVQSSAESADDIEGQILFSGISVDENTGDLIIRIIADNAQQKLLPGMYVRTQIPRQ
CEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHCCCHHHHHHCCHH
KLQAITVPEHAVRRNSAGLPFVYIVVNDKFELRPVQLEGLQQRRYVISKGVNPNDIVVVT
HHHEECCCHHHHHCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEE
GQDNLREGAELVLSDWQQ
CCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]