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The map label for this gene is pleD [H]

Identifier: 167624500

GI number: 167624500

Start: 3124625

End: 3125872

Strand: Reverse

Name: pleD [H]

Synonym: Shal_2579

Alternate gene names: 167624500

Gene position: 3125872-3124625 (Counterclockwise)

Preceding gene: 167624501

Following gene: 167624498

Centisome position: 59.8

GC content: 43.67

Gene sequence:

>1248_bases
TTGCGGATTTTAGTGGTTGAAGATAGCCAAGTGGTTTCTAGGGTGATGCGGCACCTGCTAACCCAGGAGTTGTGTTGTGA
GGTCGATGTTGCCTCTGACATGGCCAGTGCCAAGGAACTATTGGCACACAATGAGTACTTTGTTGCGATCACAGATTTAA
ATCTTCCTGATGCACAAGAAGGCGAGATAGTTAAGTTTGTTTTGGAAAAACAGATCCCTTGTATTGTATTAACTGGGAGT
TGGGATGCCGAGCAGCGAGCTCGCTTATTACAACTAGGTATCGTGGATTATGTATTTAAAGAGAACCGTTTTAGCTATGA
ATATACCGCAAAACTGGTGAAGCGCTTACGCCGTAATCAAAGTGTAAAGGTGCTTGTGGCCGATGACTCTGTCGTTAGCC
GCAAATTTATTCGTAGCTTATTAGAACAACATCTGTTTCAGGTGATAGAGGCCGATGATGGCATTTCTGCGTTAGAGACC
CTTAATGATAACCCTGACATTACCCTACTTATTACCGATTATAATATGCCAAGGCTTGATGGTTTTGGCCTCATCATTAA
GGTGCGAGAGCGACTGGGTCGTGAAGAGCTTGCTATTATCGGCCTGTCTAGTGACAGTGATGAAAGCCTGTCAGCACGCT
TTATTAAAAATGGTGCCAACGACTTCTTGCAAAAGCCATTTGTACACGAAGAGTTTCATTGTCGAGTATTAACGACACTA
GACTCGTTAGATATGGTCACTAAGTTGTGGGAACAGGCAAATCTCGATTACCTCACTAATGTGTTTAATCGGCGGTATTT
CTTTAATTTTTATCAGGAGAAGTTAACCGAGGTTGCCCAGAAACAAGGTTCTTTGTCCTTGGCTTTATTGGATATCGACT
TTTTCAAGAAGGTGAACGATACCTATGGCCATGATGTCGGTGATGAGGTGTTAATCGAATTTGCTTCGCGATTAAAGCAG
TCTTTTTCACAGCACTTCACCGTGGCGCGTATTGGTGGTGAAGAGTTTGTGGTGGGGTTTAAAGGTCTTCATATCGAAAA
GGCTTACGCCTTGATAGATAGATTCCGTATGCAACTTGAGATCACGCCGATAGAAACCTCAAAAGGGGCATTAACGATTA
CTGTCAGTACTGGAGTGACAGAGTTTGGCCATGACGAAACCGTCGATGAGTTGCTAAAGCGCGCAGATACGGGCTTATAT
GAAGCGAAGAGTGCAGGGCGTAACTTGGTTAGTATTGCAGAAAGTTAG

Upstream 100 bases:

>100_bases
CTGCAAACTTAGCCAAGTAACCAAGGTTATTGGTTATCAAGAGCCTGAAAAAGGTTTGCTAAGTGCGGGGAGAAGCAATA
TAGGGAGAAGGAGAGCAAGT

Downstream 100 bases:

>100_bases
TTTTTCAAGCTTGATTGTAACCCATAAAAAAGCAGCCAGATGGCTGCTTTTTTAATGTCTTGTCCTGAAATATGTAAACA
GATTTCAGGACAAGACATTT

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MRILVVEDSQVVSRVMRHLLTQELCCEVDVASDMASAKELLAHNEYFVAITDLNLPDAQEGEIVKFVLEKQIPCIVLTGS
WDAEQRARLLQLGIVDYVFKENRFSYEYTAKLVKRLRRNQSVKVLVADDSVVSRKFIRSLLEQHLFQVIEADDGISALET
LNDNPDITLLITDYNMPRLDGFGLIIKVRERLGREELAIIGLSSDSDESLSARFIKNGANDFLQKPFVHEEFHCRVLTTL
DSLDMVTKLWEQANLDYLTNVFNRRYFFNFYQEKLTEVAQKQGSLSLALLDIDFFKKVNDTYGHDVGDEVLIEFASRLKQ
SFSQHFTVARIGGEEFVVGFKGLHIEKAYALIDRFRMQLEITPIETSKGALTITVSTGVTEFGHDETVDELLKRADTGLY
EAKSAGRNLVSIAES

Sequences:

>Translated_415_residues
MRILVVEDSQVVSRVMRHLLTQELCCEVDVASDMASAKELLAHNEYFVAITDLNLPDAQEGEIVKFVLEKQIPCIVLTGS
WDAEQRARLLQLGIVDYVFKENRFSYEYTAKLVKRLRRNQSVKVLVADDSVVSRKFIRSLLEQHLFQVIEADDGISALET
LNDNPDITLLITDYNMPRLDGFGLIIKVRERLGREELAIIGLSSDSDESLSARFIKNGANDFLQKPFVHEEFHCRVLTTL
DSLDMVTKLWEQANLDYLTNVFNRRYFFNFYQEKLTEVAQKQGSLSLALLDIDFFKKVNDTYGHDVGDEVLIEFASRLKQ
SFSQHFTVARIGGEEFVVGFKGLHIEKAYALIDRFRMQLEITPIETSKGALTITVSTGVTEFGHDETVDELLKRADTGLY
EAKSAGRNLVSIAES
>Mature_415_residues
MRILVVEDSQVVSRVMRHLLTQELCCEVDVASDMASAKELLAHNEYFVAITDLNLPDAQEGEIVKFVLEKQIPCIVLTGS
WDAEQRARLLQLGIVDYVFKENRFSYEYTAKLVKRLRRNQSVKVLVADDSVVSRKFIRSLLEQHLFQVIEADDGISALET
LNDNPDITLLITDYNMPRLDGFGLIIKVRERLGREELAIIGLSSDSDESLSARFIKNGANDFLQKPFVHEEFHCRVLTTL
DSLDMVTKLWEQANLDYLTNVFNRRYFFNFYQEKLTEVAQKQGSLSLALLDIDFFKKVNDTYGHDVGDEVLIEFASRLKQ
SFSQHFTVARIGGEEFVVGFKGLHIEKAYALIDRFRMQLEITPIETSKGALTITVSTGVTEFGHDETVDELLKRADTGLY
EAKSAGRNLVSIAES

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=185, Percent_Identity=36.2162162162162, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787802, Length=188, Percent_Identity=32.9787234042553, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=38.0116959064327, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=38.2165605095541, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786584, Length=159, Percent_Identity=32.7044025157233, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=27.8787878787879, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=160, Percent_Identity=34.375, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=33.9506172839506, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787056, Length=164, Percent_Identity=33.5365853658537, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788381, Length=176, Percent_Identity=27.8409090909091, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787541, Length=158, Percent_Identity=29.1139240506329, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788956, Length=149, Percent_Identity=31.5436241610738, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081974, Length=176, Percent_Identity=27.2727272727273, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 47182; Mature: 47182

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVVEDSQVVSRVMRHLLTQELCCEVDVASDMASAKELLAHNEYFVAITDLNLPDAQE
CEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
GEIVKFVLEKQIPCIVLTGSWDAEQRARLLQLGIVDYVFKENRFSYEYTAKLVKRLRRNQ
CHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
SVKVLVADDSVVSRKFIRSLLEQHLFQVIEADDGISALETLNDNPDITLLITDYNMPRLD
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCCCCCC
GFGLIIKVRERLGREELAIIGLSSDSDESLSARFIKNGANDFLQKPFVHEEFHCRVLTTL
CCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHCCCCCCCCCEEEEHHH
DSLDMVTKLWEQANLDYLTNVFNRRYFFNFYQEKLTEVAQKQGSLSLALLDIDFFKKVND
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHCH
TYGHDVGDEVLIEFASRLKQSFSQHFTVARIGGEEFVVGFKGLHIEKAYALIDRFRMQLE
HCCCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHEEEE
ITPIETSKGALTITVSTGVTEFGHDETVDELLKRADTGLYEAKSAGRNLVSIAES
EEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCEEECCC
>Mature Secondary Structure
MRILVVEDSQVVSRVMRHLLTQELCCEVDVASDMASAKELLAHNEYFVAITDLNLPDAQE
CEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
GEIVKFVLEKQIPCIVLTGSWDAEQRARLLQLGIVDYVFKENRFSYEYTAKLVKRLRRNQ
CHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
SVKVLVADDSVVSRKFIRSLLEQHLFQVIEADDGISALETLNDNPDITLLITDYNMPRLD
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCCCCCC
GFGLIIKVRERLGREELAIIGLSSDSDESLSARFIKNGANDFLQKPFVHEEFHCRVLTTL
CCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHCCCCCCCCCEEEEHHH
DSLDMVTKLWEQANLDYLTNVFNRRYFFNFYQEKLTEVAQKQGSLSLALLDIDFFKKVND
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHCH
TYGHDVGDEVLIEFASRLKQSFSQHFTVARIGGEEFVVGFKGLHIEKAYALIDRFRMQLE
HCCCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHEEEE
ITPIETSKGALTITVSTGVTEFGHDETVDELLKRADTGLYEAKSAGRNLVSIAES
EEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA