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The map label for this gene is ligA [H]

Identifier: 167624496

GI number: 167624496

Start: 3116944

End: 3118947

Strand: Reverse

Name: ligA [H]

Synonym: Shal_2575

Alternate gene names: 167624496

Gene position: 3118947-3116944 (Counterclockwise)

Preceding gene: 167624497

Following gene: 167624495

Centisome position: 59.67

GC content: 48.9

Gene sequence:

>2004_bases
ATGCAAGCTATTCAAACTGAAATTGCTCAGCTCACCGCTGAACTTAATCAACACAACTACCGCTACTATGTTGATGACAG
TCCCTCTATTCCCGACTCGGAATACGACCGCTTATTGAACAGGCTGAAAGCGCTAGAGACTGAGCATCCACAATTTTGTC
TTGCCGACTCTCCGACACAAAGGGTCGGCGGCGAGGCATTAGCTAAGTTTGAGCAAATTCAACATTTGAAACCTATGCTC
AGCCTAGATAACGTCTTTGATGAACAAGAATTTACCGCGTTTAATCAACGTATTACTGATAAAACGGGCAGTATAAGTTA
CTGCTGTGAACCTAAGCTGGATGGCCTTGCGGTAAGTATTACTTACCGTGATGGTGTGTTTGAGCGAGCGGCGACCCGTG
GTGATGGTCAGACTGGTGAGAATATCACCGAAAACGTGCGCACGATTAAATCGATCCCACTGCGATTACGTGGTGATAAC
TTTCCGCTGCTAGTTGAAGTACGTGGTGAAGTGATTATGCCGCACAAGGCGTTTGATGCACTCAATGAACGTGCTCGTGC
CAAAGGTGAGAAATTGTTCGTAAACCCACGCAACGCTGCGGCGGGTAGCCTGCGCCAGTTAGACAGTAAGATCACCGCCA
GTCGCGCTTTAGGCTTTTATGCTTATGCGTTAGGAGTGGTGGAGCCGGAATCTTGGGAGCTCGCCGACAGTCATTATGGT
CAGCTTGAACAACTGCGTTCTTGGGGCGTACCCGTTAGCCAAGAGGTTAAAGTGTGTGACTCAGTCGCTCAGGTGATAGA
ATATTACAATGACATTCAACAGCGCCGCAGCAGCTTAGAGTTCGAAATTGACGGTGTGGTGATTAAGGTTAACCAAATTG
CTCATCAATTGAGTCTAGGTTTTGTCGCCAAAGCACCGCGCTGGGCAACAGCATTTAAGTTTCCTGCGCAAGAAGAGATG
ACGCTATTAGAGGGCGTCGACTTTCAGGTTGGCCGTACTGGTGCGGTGACACCGGTGGCGCGCTTAAAGCCTGTGTTTGT
CGGTGGTGTAACCGTATCAAATGCAACCTTACATAACGCAGATGAAATAGCCAGACTTGGCGTAAAAGTGGGTGATACCA
TCATTATCCGCCGCGCGGGTGACGTGATCCCGCAGATTGTTGCGGTTGTGGCGGAAAAGCGTCCTGATGATGCAAAAGAG
ATTAGCTTCCCTGCACGTTGTCCTGTGTGTGACAGTGAAGTTGAGCGCGTCGAAGGTGAAGCGGTTGCACGCTGCACTGG
TGGACTATTTTGTGAAGCGCAGCGTAAAGAGGCGATTAAGCACTTTGCATCACGCAAGGCGTTAGATATCGATGGTATGG
GCGACAAGGTTGTCGAGCAGCTTATCGATAAAGAGTTAGTCCAAAGTCCAGCCGATCTATTTAAGTTGACCGCATCTGCC
ATGACGATGCTAGAGCGTATGGGAATGAAGTCGGCGACTAAGCTTGTTGCGGCTATCGATGTCGCTAAACAAACCACGTT
TGCTCGCTTCCTTTATGCGCTAGGAATACGCGAAGTGGGTGAGGCGACAGCTGCGAACTTGGCGGCATACTTTAAGACGC
TCGATGCACTCAAAGCGGCAAATGAGGAAACCTTTATTAAGGTCGATGATGTCGGCACCATTGTGGCGGCGCACTTAGCT
CACTTTCTTGCCCAGCCACATAACTTGGAAGTCATTGATAAGCTGATTGAAGCAGGCGTACATTGGCCAGCAATTGAAGA
GGTTGCAGAAGAAGACCTTTCTCTGAAAGGGCAAACTTGGGTATTAACAGGAACCTTAACTCAATTAAACCGAAATGACG
CCAAAGCCAAGCTACAGTTGTTAGGTGCTAAGGTGGCGGGTAGCGTATCGAAAAATACTGATTGTCTCGTTGCTGGTGCC
GCTGCTGGGTCAAAACTGGCTAAGGCGCAGGAGCTAGGGGTTAAGGTTATCGATGAAGAAGCCTTGATTGCTATCTTGTC
ATAG

Upstream 100 bases:

>100_bases
AAACACATTAGGGTAGTTTGAGTCTACATTCTAGGCTTTTGAAATATATACACATTTGAAAGGCCGCAGTAGCGGCCTTT
GTTATTATTTGGACATTAAG

Downstream 100 bases:

>100_bases
TGACCCATTAATTTTAAAAAAGCTCCTTAGGGAGCTTTTTTAATGCTTGAAAACTGTCGATTTGTCGCCATATCTAGTTG
TAGGTTTAGCAGCATAGTTG

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MQAIQTEIAQLTAELNQHNYRYYVDDSPSIPDSEYDRLLNRLKALETEHPQFCLADSPTQRVGGEALAKFEQIQHLKPML
SLDNVFDEQEFTAFNQRITDKTGSISYCCEPKLDGLAVSITYRDGVFERAATRGDGQTGENITENVRTIKSIPLRLRGDN
FPLLVEVRGEVIMPHKAFDALNERARAKGEKLFVNPRNAAAGSLRQLDSKITASRALGFYAYALGVVEPESWELADSHYG
QLEQLRSWGVPVSQEVKVCDSVAQVIEYYNDIQQRRSSLEFEIDGVVIKVNQIAHQLSLGFVAKAPRWATAFKFPAQEEM
TLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIARLGVKVGDTIIIRRAGDVIPQIVAVVAEKRPDDAKE
ISFPARCPVCDSEVERVEGEAVARCTGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKELVQSPADLFKLTASA
MTMLERMGMKSATKLVAAIDVAKQTTFARFLYALGIREVGEATAANLAAYFKTLDALKAANEETFIKVDDVGTIVAAHLA
HFLAQPHNLEVIDKLIEAGVHWPAIEEVAEEDLSLKGQTWVLTGTLTQLNRNDAKAKLQLLGAKVAGSVSKNTDCLVAGA
AAGSKLAKAQELGVKVIDEEALIAILS

Sequences:

>Translated_667_residues
MQAIQTEIAQLTAELNQHNYRYYVDDSPSIPDSEYDRLLNRLKALETEHPQFCLADSPTQRVGGEALAKFEQIQHLKPML
SLDNVFDEQEFTAFNQRITDKTGSISYCCEPKLDGLAVSITYRDGVFERAATRGDGQTGENITENVRTIKSIPLRLRGDN
FPLLVEVRGEVIMPHKAFDALNERARAKGEKLFVNPRNAAAGSLRQLDSKITASRALGFYAYALGVVEPESWELADSHYG
QLEQLRSWGVPVSQEVKVCDSVAQVIEYYNDIQQRRSSLEFEIDGVVIKVNQIAHQLSLGFVAKAPRWATAFKFPAQEEM
TLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIARLGVKVGDTIIIRRAGDVIPQIVAVVAEKRPDDAKE
ISFPARCPVCDSEVERVEGEAVARCTGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKELVQSPADLFKLTASA
MTMLERMGMKSATKLVAAIDVAKQTTFARFLYALGIREVGEATAANLAAYFKTLDALKAANEETFIKVDDVGTIVAAHLA
HFLAQPHNLEVIDKLIEAGVHWPAIEEVAEEDLSLKGQTWVLTGTLTQLNRNDAKAKLQLLGAKVAGSVSKNTDCLVAGA
AAGSKLAKAQELGVKVIDEEALIAILS
>Mature_667_residues
MQAIQTEIAQLTAELNQHNYRYYVDDSPSIPDSEYDRLLNRLKALETEHPQFCLADSPTQRVGGEALAKFEQIQHLKPML
SLDNVFDEQEFTAFNQRITDKTGSISYCCEPKLDGLAVSITYRDGVFERAATRGDGQTGENITENVRTIKSIPLRLRGDN
FPLLVEVRGEVIMPHKAFDALNERARAKGEKLFVNPRNAAAGSLRQLDSKITASRALGFYAYALGVVEPESWELADSHYG
QLEQLRSWGVPVSQEVKVCDSVAQVIEYYNDIQQRRSSLEFEIDGVVIKVNQIAHQLSLGFVAKAPRWATAFKFPAQEEM
TLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIARLGVKVGDTIIIRRAGDVIPQIVAVVAEKRPDDAKE
ISFPARCPVCDSEVERVEGEAVARCTGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKELVQSPADLFKLTASA
MTMLERMGMKSATKLVAAIDVAKQTTFARFLYALGIREVGEATAANLAAYFKTLDALKAANEETFIKVDDVGTIVAAHLA
HFLAQPHNLEVIDKLIEAGVHWPAIEEVAEEDLSLKGQTWVLTGTLTQLNRNDAKAKLQLLGAKVAGSVSKNTDCLVAGA
AAGSKLAKAQELGVKVIDEEALIAILS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=60.9538002980626, Blast_Score=855, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=522, Percent_Identity=23.1800766283525, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 72974; Mature: 72974

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAIQTEIAQLTAELNQHNYRYYVDDSPSIPDSEYDRLLNRLKALETEHPQFCLADSPTQ
CCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCHH
RVGGEALAKFEQIQHLKPMLSLDNVFDEQEFTAFNQRITDKTGSISYCCEPKLDGLAVSI
HCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
TYRDGVFERAATRGDGQTGENITENVRTIKSIPLRLRGDNFPLLVEVRGEVIMPHKAFDA
EECCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEECCCEECCHHHHHH
LNERARAKGEKLFVNPRNAAAGSLRQLDSKITASRALGFYAYALGVVEPESWELADSHYG
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
QLEQLRSWGVPVSQEVKVCDSVAQVIEYYNDIQQRRSSLEFEIDGVVIKVNQIAHQLSLG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEHHHHHHHHHHH
FVAKAPRWATAFKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNA
HHCCCCCCHHEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEECEEECCCCCCCH
DEIARLGVKVGDTIIIRRAGDVIPQIVAVVAEKRPDDAKEISFPARCPVCDSEVERVEGE
HHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHCCH
AVARCTGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKELVQSPADLFKLTASA
HHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
MTMLERMGMKSATKLVAAIDVAKQTTFARFLYALGIREVGEATAANLAAYFKTLDALKAA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEETFIKVDDVGTIVAAHLAHFLAQPHNLEVIDKLIEAGVHWPAIEEVAEEDLSLKGQTW
CCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEE
VLTGTLTQLNRNDAKAKLQLLGAKVAGSVSKNTDCLVAGAAAGSKLAKAQELGVKVIDEE
EEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCEEECCC
ALIAILS
CEEEEEC
>Mature Secondary Structure
MQAIQTEIAQLTAELNQHNYRYYVDDSPSIPDSEYDRLLNRLKALETEHPQFCLADSPTQ
CCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCHH
RVGGEALAKFEQIQHLKPMLSLDNVFDEQEFTAFNQRITDKTGSISYCCEPKLDGLAVSI
HCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
TYRDGVFERAATRGDGQTGENITENVRTIKSIPLRLRGDNFPLLVEVRGEVIMPHKAFDA
EECCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEECCCEECCHHHHHH
LNERARAKGEKLFVNPRNAAAGSLRQLDSKITASRALGFYAYALGVVEPESWELADSHYG
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
QLEQLRSWGVPVSQEVKVCDSVAQVIEYYNDIQQRRSSLEFEIDGVVIKVNQIAHQLSLG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEHHHHHHHHHHH
FVAKAPRWATAFKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNA
HHCCCCCCHHEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEECEEECCCCCCCH
DEIARLGVKVGDTIIIRRAGDVIPQIVAVVAEKRPDDAKEISFPARCPVCDSEVERVEGE
HHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHCCH
AVARCTGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKELVQSPADLFKLTASA
HHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
MTMLERMGMKSATKLVAAIDVAKQTTFARFLYALGIREVGEATAANLAAYFKTLDALKAA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEETFIKVDDVGTIVAAHLAHFLAQPHNLEVIDKLIEAGVHWPAIEEVAEEDLSLKGQTW
CCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEE
VLTGTLTQLNRNDAKAKLQLLGAKVAGSVSKNTDCLVAGAAAGSKLAKAQELGVKVIDEE
EEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCEEECCC
ALIAILS
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA