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The map label for this gene is leuO [H]

Identifier: 167624456

GI number: 167624456

Start: 3064236

End: 3065150

Strand: Reverse

Name: leuO [H]

Synonym: Shal_2534

Alternate gene names: 167624456

Gene position: 3065150-3064236 (Counterclockwise)

Preceding gene: 167624461

Following gene: 167624455

Centisome position: 58.64

GC content: 43.5

Gene sequence:

>915_bases
GTGAGTAGCAATATAGATAAAAGATTCGACTTTAATTTGTTGGCGATATTCTTAGAGGTTTATCAGTTACGTTCTATTAC
TTTAGCTGCTGAATCCTTAGGCTTAACTCAACCCGGTGTAAGTGCGGCATTAAAGCGCCTACAAGCTAAACTCGGTGTGG
AGCTTTTTGTTCGTGAAGGTCGTGGTATTTCACCTACCAACGCGGCTATTCAGCTAGCAAGTCAAGTCTCTCCAGCCTAT
AACACTGTTAACAGTGCGCTGAGCAATGTAGTCGGTTTTGATAAGCTGACTCCGCGTTCGTTTCTTGTCTATGTCGATGA
ACCGATAATGAATTTGCTGCAACCCTTAGTTGAGCGAGATAGCGATATGGGCAATTGTGAGATTATGTTTCAACTACCGC
CGATTGAAGAGGATCAACTGCTTCATCAACTGAGTTTGCAAAAGGCCGATCTGGCAATCGACATTGGTCAACTACCGAGC
CTCTCCTACACTAATCAACTCTTTTACCGTGAAAGTGTCACTATGATCTGCCGTAAGGGTCATCCGCGTATTAATGGCAA
GATAGACAAATCCCAGTTTTACCAAGAGAAGCATATTACGTTGAAGCTTCGCCGTGCGACGCTGTCTGCACTGGATTTTT
TTACCGAGGAGGTGATCCGTGAACGCAAGGTTAGCTGTGAGTGTCACTCATTACTCTCTATGATGGCGCTCGTTTCTGAG
AGTGACTGTATTGCTATCACCACAAGGAGTACCGCTGATAAATATGCCGAGAAATTTGGCCTTCAAAGTATCGAACTGCC
CTTTAACACTAAACCTGTTCTACACAATATGATGTGGCATAAACGCAATGAGTTTAACCCAGCACATACTTGGCTTAGAG
AAAAGCTAAACCAATTGATGAAGCAATGGAAATAG

Upstream 100 bases:

>100_bases
CTAACCAATAAACTAAGCTTTAGATGATACATAACGATTTTGTATGTATTGGTATTGGTATGTATGATTGTTACTTCTGT
ATTAATGGCGGGAGTATTGT

Downstream 100 bases:

>100_bases
TTATATATTTACAATCCGTTAACTATTACACATGCCGCTAAGCAGAGAACCTTAAAATGGACTTCATTGAAATATACCCA
GATGCGATTCCAGCTGAATT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSSNIDKRFDFNLLAIFLEVYQLRSITLAAESLGLTQPGVSAALKRLQAKLGVELFVREGRGISPTNAAIQLASQVSPAY
NTVNSALSNVVGFDKLTPRSFLVYVDEPIMNLLQPLVERDSDMGNCEIMFQLPPIEEDQLLHQLSLQKADLAIDIGQLPS
LSYTNQLFYRESVTMICRKGHPRINGKIDKSQFYQEKHITLKLRRATLSALDFFTEEVIRERKVSCECHSLLSMMALVSE
SDCIAITTRSTADKYAEKFGLQSIELPFNTKPVLHNMMWHKRNEFNPAHTWLREKLNQLMKQWK

Sequences:

>Translated_304_residues
MSSNIDKRFDFNLLAIFLEVYQLRSITLAAESLGLTQPGVSAALKRLQAKLGVELFVREGRGISPTNAAIQLASQVSPAY
NTVNSALSNVVGFDKLTPRSFLVYVDEPIMNLLQPLVERDSDMGNCEIMFQLPPIEEDQLLHQLSLQKADLAIDIGQLPS
LSYTNQLFYRESVTMICRKGHPRINGKIDKSQFYQEKHITLKLRRATLSALDFFTEEVIRERKVSCECHSLLSMMALVSE
SDCIAITTRSTADKYAEKFGLQSIELPFNTKPVLHNMMWHKRNEFNPAHTWLREKLNQLMKQWK
>Mature_303_residues
SSNIDKRFDFNLLAIFLEVYQLRSITLAAESLGLTQPGVSAALKRLQAKLGVELFVREGRGISPTNAAIQLASQVSPAYN
TVNSALSNVVGFDKLTPRSFLVYVDEPIMNLLQPLVERDSDMGNCEIMFQLPPIEEDQLLHQLSLQKADLAIDIGQLPSL
SYTNQLFYRESVTMICRKGHPRINGKIDKSQFYQEKHITLKLRRATLSALDFFTEEVIRERKVSCECHSLLSMMALVSES
DCIAITTRSTADKYAEKFGLQSIELPFNTKPVLHNMMWHKRNEFNPAHTWLREKLNQLMKQWK

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=296, Percent_Identity=29.0540540540541, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI87081766, Length=295, Percent_Identity=24.7457627118644, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786820, Length=299, Percent_Identity=27.4247491638796, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1790147, Length=302, Percent_Identity=22.8476821192053, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34653; Mature: 34521

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNIDKRFDFNLLAIFLEVYQLRSITLAAESLGLTQPGVSAALKRLQAKLGVELFVREG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHEEEECC
RGISPTNAAIQLASQVSPAYNTVNSALSNVVGFDKLTPRSFLVYVDEPIMNLLQPLVERD
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHCC
SDMGNCEIMFQLPPIEEDQLLHQLSLQKADLAIDIGQLPSLSYTNQLFYRESVTMICRKG
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCC
HPRINGKIDKSQFYQEKHITLKLRRATLSALDFFTEEVIRERKVSCECHSLLSMMALVSE
CCCCCCEECHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
SDCIAITTRSTADKYAEKFGLQSIELPFNTKPVLHNMMWHKRNEFNPAHTWLREKLNQLM
CCEEEEEECCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
KQWK
HHCC
>Mature Secondary Structure 
SSNIDKRFDFNLLAIFLEVYQLRSITLAAESLGLTQPGVSAALKRLQAKLGVELFVREG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHEEEECC
RGISPTNAAIQLASQVSPAYNTVNSALSNVVGFDKLTPRSFLVYVDEPIMNLLQPLVERD
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHCC
SDMGNCEIMFQLPPIEEDQLLHQLSLQKADLAIDIGQLPSLSYTNQLFYRESVTMICRKG
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCC
HPRINGKIDKSQFYQEKHITLKLRRATLSALDFFTEEVIRERKVSCECHSLLSMMALVSE
CCCCCCEECHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
SDCIAITTRSTADKYAEKFGLQSIELPFNTKPVLHNMMWHKRNEFNPAHTWLREKLNQLM
CCEEEEEECCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
KQWK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3413113; 1630901; 9278503; 3519576 [H]