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The map label for this gene is sucB [H]

Identifier: 167624410

GI number: 167624410

Start: 2990533

End: 2991729

Strand: Reverse

Name: sucB [H]

Synonym: Shal_2488

Alternate gene names: 167624410

Gene position: 2991729-2990533 (Counterclockwise)

Preceding gene: 167624411

Following gene: 167624409

Centisome position: 57.24

GC content: 45.61

Gene sequence:

>1197_bases
ATGAGTATCGAAATTAAGGTACCCGTACTGCCAGAATCAGTTGCAGATGCAACCATTGCTACATGGCATGTTCAAGCTGG
CGAGCAAGTTTCTCGTGATCAAATCCTTGTTGATATTGAAACTGATAAAGTCGTACTTGAAGTTGTTGCACCAGAAGATG
GACAAATTGCTGAGTTTTTGGCTCAGGAAGGTGACACTGTACTTGGTGAGGCAGTGATTGCTAGCTTCGTAGCTGGCGCT
GTTGCAGGTCAAGAAGTGACCAAAGCTCAAGCAGAAGCCGCTGCGCCAACAAGCGAAGCATCTGATGAGTCAAACGATGC
GCTAAGCCCATCTGTTCGTCGTCTTATCGCAGAGCATAATGTTGAAGCAAGTGCTGTTAAAGGTACTGGCGTTGGTGGAC
GCATCACTAAAGATGACGTTGAAGCTTTCGTTAAAAATAAACCCGCTGCAGCGCCTGCGAGCACATCAGCCCCAGCTGTT
GTTGCTCCTTTAGCCGAGCGTAGCGAGAAGCGTGTTCCGATGACTCGCCTTCGTAAGACGATTGCTAACCGTCTACTAGA
AGCTAAAAACTCTACTGCCATGCTAACGACGTTTAACGAAGTTAACATGAAGCCAATCATGGATATTCGTAAGCAGTATC
AAGAGATTTTCGAAAAACGTCATGGTATTCGTCTAGGTTTCATGTCCTTCTATGTTAAGGCTGTGACAGAAGCACTTAAG
CGCTTCCCTGAAGTAAACGCATCAATCGATGGTAACGATATTGTTTATCACAACTACTTTGATATCAGTATTGCAGTGTC
GACTCCTCGTGGTCTTGTGACACCTGTACTACGTGACACTGACAAGATGAGTCTTGCCGATATCGAACGTAACGTTCGTG
AACTCGCGATTAAAGGTCGCGACGGCAAGTTAACGGTTGATGATATGACTGGCGGTAACTTCACCGTGACTAACGGTGGG
GTATTTGGCTCATTGATGTCAACGCCAATCTTGAATTTGCCACAAAGTGCGATCTTAGGCATGCATGCCATTAAAGATCG
CCCTATGGCAGTAAATGGTCAGGTTGAAATCCTGCCTATGATGTACCTCGCACTTTCTTACGATCACCGTATTGTAGATG
GACGTGAATCAGTTGGTTACTTGGTTGCGATTAAAGACTTCCTTGAAGATCCAACTCGTCTGCTTCTTGACCTATAA

Upstream 100 bases:

>100_bases
CATGTGGTTACCCTGCATTGCATGCTCAGCAACAAGAATCATTAATCAACAGTGCGTTAAAGCTGTAGTAATAGACATTA
TATAAAAAGGATCGTAATCC

Downstream 100 bases:

>100_bases
GTCAAGACAGACAATACCCTAGCTGCAGGCCCGTTTGGGCCTGCTGAAATACATAAGAAGTAGAATGGATAGATCATCAT
GAATTTGCACGAATATCAGG

Product: 2-oxoglutarate dehydrogenase, E2 subunit, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSIEIKVPVLPESVADATIATWHVQAGEQVSRDQILVDIETDKVVLEVVAPEDGQIAEFLAQEGDTVLGEAVIASFVAGA
VAGQEVTKAQAEAAAPTSEASDESNDALSPSVRRLIAEHNVEASAVKGTGVGGRITKDDVEAFVKNKPAAAPASTSAPAV
VAPLAERSEKRVPMTRLRKTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQEIFEKRHGIRLGFMSFYVKAVTEALK
RFPEVNASIDGNDIVYHNYFDISIAVSTPRGLVTPVLRDTDKMSLADIERNVRELAIKGRDGKLTVDDMTGGNFTVTNGG
VFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIVDGRESVGYLVAIKDFLEDPTRLLLDL

Sequences:

>Translated_398_residues
MSIEIKVPVLPESVADATIATWHVQAGEQVSRDQILVDIETDKVVLEVVAPEDGQIAEFLAQEGDTVLGEAVIASFVAGA
VAGQEVTKAQAEAAAPTSEASDESNDALSPSVRRLIAEHNVEASAVKGTGVGGRITKDDVEAFVKNKPAAAPASTSAPAV
VAPLAERSEKRVPMTRLRKTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQEIFEKRHGIRLGFMSFYVKAVTEALK
RFPEVNASIDGNDIVYHNYFDISIAVSTPRGLVTPVLRDTDKMSLADIERNVRELAIKGRDGKLTVDDMTGGNFTVTNGG
VFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIVDGRESVGYLVAIKDFLEDPTRLLLDL
>Mature_397_residues
SIEIKVPVLPESVADATIATWHVQAGEQVSRDQILVDIETDKVVLEVVAPEDGQIAEFLAQEGDTVLGEAVIASFVAGAV
AGQEVTKAQAEAAAPTSEASDESNDALSPSVRRLIAEHNVEASAVKGTGVGGRITKDDVEAFVKNKPAAAPASTSAPAVV
APLAERSEKRVPMTRLRKTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQEIFEKRHGIRLGFMSFYVKAVTEALKR
FPEVNASIDGNDIVYHNYFDISIAVSTPRGLVTPVLRDTDKMSLADIERNVRELAIKGRDGKLTVDDMTGGNFTVTNGGV
FGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIVDGRESVGYLVAIKDFLEDPTRLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=55.5118110236221, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=30.8755760368664, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI110671329, Length=419, Percent_Identity=26.7303102625298, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=26.6075388026608, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=26.6075388026608, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=33.5403726708075, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=69.9751861042184, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1786305, Length=407, Percent_Identity=32.9238329238329, Blast_Score=174, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=408, Percent_Identity=41.1764705882353, Blast_Score=304, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI17560088, Length=437, Percent_Identity=31.350114416476, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=25, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=29.8076923076923, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=402, Percent_Identity=42.0398009950249, Blast_Score=315, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=24.9443207126949, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24645909, Length=228, Percent_Identity=59.6491228070175, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI18859875, Length=419, Percent_Identity=26.7303102625298, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24582497, Length=292, Percent_Identity=31.5068493150685, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI20129315, Length=292, Percent_Identity=31.5068493150685, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43113; Mature: 42982

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEIKVPVLPESVADATIATWHVQAGEQVSRDQILVDIETDKVVLEVVAPEDGQIAEFL
CEEEEEECCCCCHHCCCEEEEEEECCCCCCCCCEEEEEEECCEEEEEEECCCCCHHHHHH
AQEGDTVLGEAVIASFVAGAVAGQEVTKAQAEAAAPTSEASDESNDALSPSVRRLIAEHN
HHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC
VEASAVKGTGVGGRITKDDVEAFVKNKPAAAPASTSAPAVVAPLAERSEKRVPMTRLRKT
CCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHCCCCCCCHHHHHHH
IANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQEIFEKRHGIRLGFMSFYVKAVTEALK
HHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHH
RFPEVNASIDGNDIVYHNYFDISIAVSTPRGLVTPVLRDTDKMSLADIERNVRELAIKGR
HCCCCCCCCCCCEEEEEEEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHEEECC
DGKLTVDDMTGGNFTVTNGGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPM
CCEEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCEEHHHH
MYLALSYDHRIVDGRESVGYLVAIKDFLEDPTRLLLDL
HHHHHCCCCEEECCCCCCCCEEEEHHHHCCHHHHHHCC
>Mature Secondary Structure 
SIEIKVPVLPESVADATIATWHVQAGEQVSRDQILVDIETDKVVLEVVAPEDGQIAEFL
EEEEEECCCCCHHCCCEEEEEEECCCCCCCCCEEEEEEECCEEEEEEECCCCCHHHHHH
AQEGDTVLGEAVIASFVAGAVAGQEVTKAQAEAAAPTSEASDESNDALSPSVRRLIAEHN
HHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC
VEASAVKGTGVGGRITKDDVEAFVKNKPAAAPASTSAPAVVAPLAERSEKRVPMTRLRKT
CCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHCCCCCCCHHHHHHH
IANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQEIFEKRHGIRLGFMSFYVKAVTEALK
HHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHH
RFPEVNASIDGNDIVYHNYFDISIAVSTPRGLVTPVLRDTDKMSLADIERNVRELAIKGR
HCCCCCCCCCCCEEEEEEEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHEEECC
DGKLTVDDMTGGNFTVTNGGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPM
CCEEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCEEHHHH
MYLALSYDHRIVDGRESVGYLVAIKDFLEDPTRLLLDL
HHHHHCCCCEEECCCCCCCCEEEEHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]