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The map label for this gene is fumB [H]

Identifier: 167624180

GI number: 167624180

Start: 2736908

End: 2738428

Strand: Reverse

Name: fumB [H]

Synonym: Shal_2256

Alternate gene names: 167624180

Gene position: 2738428-2736908 (Counterclockwise)

Preceding gene: 167624183

Following gene: 167624179

Centisome position: 52.39

GC content: 47.47

Gene sequence:

>1521_bases
GTGAGCAAAGAAGTCCCTGTAATCAAGCAAGCCGATCTCATTGAAAGCGTTGCTGATGCCCTACAATATATCTCTTATTA
TCACCCGAAAGACTTTGTAGATTCTATGGCCGAGGCCTATGAGCGAGAACAAAGCGCTGCCGCCAAGGACGCGATTGCAC
AAATTCTTATCAATTCTCGCATGTCTGCAGAAGGTAAACGTCCACTTTGCCAAGATACGGGTATTGTCACCTGCTTTGTA
AAAATCGGCATGGGTGTTCAATGGGATAAGACAGATTTAACCGTTCAAGAGATGGTAGATGAAGGCGTACGCCGTGCTTA
TACCAATCCCGATAACCCGCTGCGCGCATCGATTGTTGCCGATCCTGCTGGTGGCCGAAAGAACACCAAAGACAATACAC
CGTCAGTTGTGCATGTTGAAATGGTTCCGGGTAACCATGTTGATGTCGCTATTGCAGCAAAAGGTGGGGGATCTGAGAAC
AAGTCTAAAATGGCTATGCTTAATCCATCTGATGATATCGCAGCTTGGGTAGAGAAAACACTCCCTACAATGGGCGCCGG
ATGGTGTCCACCGGGTATGCTTGGCATAGGTATCGGTGGTACCGCAGAAAAAGCGGCGGTTATGGCTAAAGAGTCATTAA
TGGATCCAGTTGATATCCATGAGTTACAAGCTCGCGGCGCCAAAACAACCGATGAAAAGCTACGTCTAGATATTTTTGAT
CGCTGCAACAAGCTAGGTATTGGCGCGCAAGGTCTAGGCGGTGTGACCACTGTTTTAGATATTAAAATTAAATCCGCTCC
AACCCACGCGGCCTCAAAGCCTGTTGTGATGATCCCTAATTGCGCAGCGACTCGTCATGTGCATTTTCACCTCGATGGCA
CAGGTCCTGCTGAACTCACTCCACCATCACTTGAAGATTGGCCTGACATTACCTGGGAAGTGGGTGAGAGCGTTCGCCGT
GTTGATTTAAACACAGTGACTCAAACTGAGATGGAGCAGTGGAAGAGTGGTGATACTTTATTACTAAACGGCAAGATGCT
CACCGGTCGTGACGCCGCGCATAAACGTATTCAAAGCTTAATTGAAAGCGGTGAAGGCTTGCCTGAAGGAGTGGATTTTA
AAGGTAAGTTTATCTATTACGTTGGTCCTGTCGATCCTGTCGGTGATGAGGTTGTAGGTCCGGCTGGCCCAACGACAGCA
ACGCGCATGGACAAGTTCACTGATTTGATGCTTGATAAAACAGGCCTGATGGGCATGATTGGTAAAGCCGAGCGTGGTCC
TGCAACGGTTGAGTCAATTAAGAAGCATAAATCATGTTACCTAATGGCCGTAGGCGGCGCAGCGTATCTCGTCTCTAAGG
CAATTAAAAAGTCTCGTGTTGTCGCCTTCGAAGATTTAGGTATGGAGGCAATCTATGAGTTTGATGTTAAAGATATGCCA
GTCACGGTAGCGGTAGATTCTGAAGGCGTCAATGCACATGAAACTGGGCCTGCTATTTGGAAGGTTAAGTTAGAAGGCTA
A

Upstream 100 bases:

>100_bases
CAAAACACGTGAGCTTTAATGGCGACTGAAGTCATTTGCACTTGCAAGTACGAGTGTGGTTTCAGCCGATTAATAAGTGT
CTGCTAAAAAGGAAGTGTCT

Downstream 100 bases:

>100_bases
TGTTTGAGTGAAAGTGCAAACTTAGTCTAAAGCAACGGATTAGAACAATGGCTTAGGCGTATTGCTTAAGCCATTTATTT
TGGCTACTGTGTCGGGTCTC

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase subunit alpha

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MSKEVPVIKQADLIESVADALQYISYYHPKDFVDSMAEAYEREQSAAAKDAIAQILINSRMSAEGKRPLCQDTGIVTCFV
KIGMGVQWDKTDLTVQEMVDEGVRRAYTNPDNPLRASIVADPAGGRKNTKDNTPSVVHVEMVPGNHVDVAIAAKGGGSEN
KSKMAMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVMAKESLMDPVDIHELQARGAKTTDEKLRLDIFD
RCNKLGIGAQGLGGVTTVLDIKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPAELTPPSLEDWPDITWEVGESVRR
VDLNTVTQTEMEQWKSGDTLLLNGKMLTGRDAAHKRIQSLIESGEGLPEGVDFKGKFIYYVGPVDPVGDEVVGPAGPTTA
TRMDKFTDLMLDKTGLMGMIGKAERGPATVESIKKHKSCYLMAVGGAAYLVSKAIKKSRVVAFEDLGMEAIYEFDVKDMP
VTVAVDSEGVNAHETGPAIWKVKLEG

Sequences:

>Translated_506_residues
MSKEVPVIKQADLIESVADALQYISYYHPKDFVDSMAEAYEREQSAAAKDAIAQILINSRMSAEGKRPLCQDTGIVTCFV
KIGMGVQWDKTDLTVQEMVDEGVRRAYTNPDNPLRASIVADPAGGRKNTKDNTPSVVHVEMVPGNHVDVAIAAKGGGSEN
KSKMAMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVMAKESLMDPVDIHELQARGAKTTDEKLRLDIFD
RCNKLGIGAQGLGGVTTVLDIKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPAELTPPSLEDWPDITWEVGESVRR
VDLNTVTQTEMEQWKSGDTLLLNGKMLTGRDAAHKRIQSLIESGEGLPEGVDFKGKFIYYVGPVDPVGDEVVGPAGPTTA
TRMDKFTDLMLDKTGLMGMIGKAERGPATVESIKKHKSCYLMAVGGAAYLVSKAIKKSRVVAFEDLGMEAIYEFDVKDMP
VTVAVDSEGVNAHETGPAIWKVKLEG
>Mature_505_residues
SKEVPVIKQADLIESVADALQYISYYHPKDFVDSMAEAYEREQSAAAKDAIAQILINSRMSAEGKRPLCQDTGIVTCFVK
IGMGVQWDKTDLTVQEMVDEGVRRAYTNPDNPLRASIVADPAGGRKNTKDNTPSVVHVEMVPGNHVDVAIAAKGGGSENK
SKMAMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVMAKESLMDPVDIHELQARGAKTTDEKLRLDIFDR
CNKLGIGAQGLGGVTTVLDIKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPAELTPPSLEDWPDITWEVGESVRRV
DLNTVTQTEMEQWKSGDTLLLNGKMLTGRDAAHKRIQSLIESGEGLPEGVDFKGKFIYYVGPVDPVGDEVVGPAGPTTAT
RMDKFTDLMLDKTGLMGMIGKAERGPATVESIKKHKSCYLMAVGGAAYLVSKAIKKSRVVAFEDLGMEAIYEFDVKDMPV
TVAVDSEGVNAHETGPAIWKVKLEG

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=472, Percent_Identity=32.6271186440678, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787897, Length=471, Percent_Identity=33.5456475583864, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1789442, Length=280, Percent_Identity=27.8571428571429, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=31.2849162011173, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54401; Mature: 54270

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEVPVIKQADLIESVADALQYISYYHPKDFVDSMAEAYEREQSAAAKDAIAQILINSR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MSAEGKRPLCQDTGIVTCFVKIGMGVQWDKTDLTVQEMVDEGVRRAYTNPDNPLRASIVA
CCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
DPAGGRKNTKDNTPSVVHVEMVPGNHVDVAIAAKGGGSENKSKMAMLNPSDDIAAWVEKT
CCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHH
LPTMGAGWCPPGMLGIGIGGTAEKAAVMAKESLMDPVDIHELQARGAKTTDEKLRLDIFD
CCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHEEEEHHH
RCNKLGIGAQGLGGVTTVLDIKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPAELT
CCHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCCCC
PPSLEDWPDITWEVGESVRRVDLNTVTQTEMEQWKSGDTLLLNGKMLTGRDAAHKRIQSL
CCCCCCCCCCHHHHCCHHHEECCCHHHHHHHHHHCCCCEEEEECEEECCCHHHHHHHHHH
IESGEGLPEGVDFKGKFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTDLMLDKTGLMGMI
HHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHC
GKAERGPATVESIKKHKSCYLMAVGGAAYLVSKAIKKSRVVAFEDLGMEAIYEFDVKDMP
CCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCEEEEHHCCCCEEEEECCCCCC
VTVAVDSEGVNAHETGPAIWKVKLEG
EEEEECCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
SKEVPVIKQADLIESVADALQYISYYHPKDFVDSMAEAYEREQSAAAKDAIAQILINSR
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MSAEGKRPLCQDTGIVTCFVKIGMGVQWDKTDLTVQEMVDEGVRRAYTNPDNPLRASIVA
CCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
DPAGGRKNTKDNTPSVVHVEMVPGNHVDVAIAAKGGGSENKSKMAMLNPSDDIAAWVEKT
CCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHH
LPTMGAGWCPPGMLGIGIGGTAEKAAVMAKESLMDPVDIHELQARGAKTTDEKLRLDIFD
CCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHEEEEHHH
RCNKLGIGAQGLGGVTTVLDIKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPAELT
CCHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCCCC
PPSLEDWPDITWEVGESVRRVDLNTVTQTEMEQWKSGDTLLLNGKMLTGRDAAHKRIQSL
CCCCCCCCCCHHHHCCHHHEECCCHHHHHHHHHHCCCCEEEEECEEECCCHHHHHHHHHH
IESGEGLPEGVDFKGKFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTDLMLDKTGLMGMI
HHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHC
GKAERGPATVESIKKHKSCYLMAVGGAAYLVSKAIKKSRVVAFEDLGMEAIYEFDVKDMP
CCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCEEEEHHCCCCEEEEECCCCCC
VTVAVDSEGVNAHETGPAIWKVKLEG
EEEEECCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]