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The map label for this gene is paaF [C]

Identifier: 167624051

GI number: 167624051

Start: 2561948

End: 2562904

Strand: Direct

Name: paaF [C]

Synonym: Shal_2127

Alternate gene names: 167624051

Gene position: 2561948-2562904 (Clockwise)

Preceding gene: 167624050

Following gene: 167624052

Centisome position: 49.01

GC content: 46.92

Gene sequence:

>957_bases
ATGCCTAACAATACAGCTCACAGTGAAGCGGCCGCCCAAACTACGGCTAACCAGCTAGCGTCGCTTGATGGATGTCACCG
CGAAGATCTTGATGGCGTTGTGTTTTATGAAGTGCGGGAACAAGTCGCAATCGTAACCATGAATCGCCCTGAATATAACA
ATGCACAAAATTCTAAAATGACCTACGCGCTCGACGCGGCTTTTAAACGAGCGTGCGATGATGACAACGTAAAAGTGATC
ATACTCGCAGGTGAAGGTAAGCACTTTTCAGCCGGACATGACATCGGCACTCCTGGACGCGACGTTGACCAAGAGTTTGA
TCGTGCCAGTCTTTGGTACGACCACAGCAATAAAAGTGGTGGTGAGTTTTTATATGCTAGAGAGCAAGAGGTTTACTTAG
GCATGTGTCGCCGCTGGCGTGATATGCCAAAACCCACTATTGCAATGGTACAGGGTGCCTGTATTGCCGGAGGGCTAATG
TTAGCCTGGGTGTGCGATCTTATTATCTGCTCTGACGATGCCTATTACTGTGACCCGGTTGTTCGAATGGGGATCCCGGG
CGTTGAATACTTTGCGCACGTGCATGAGCTTAACCCTCGAATCGCAAAAGAGTTTTTATTCTTAGGCGACAAAATGAATG
CTCAGCGTGCCTATGAGTTAGGCATGGTGAACCGAATATTTGCACGAAAAGATTTAACTGAAGAAACGTTAACCATAGCT
AATAAAATCGCCCAAATGCCCCGACTTGGTCTGCAATTAACTAAGCAAGCCATTAATAACGCCGAAGATCTGATGGGCAA
ACGTAGCACGATGGATATGGTTTTTGGCTTACACCATTTTGCTCATGTTCATAATGAGTCAATATCCGGTGACAAGCTCG
GTGGTTTCGATGCAAAAGCGATGGCAAAAGCCAATAAGGCCGCAAGTGTGTCAGATAAGCGAAAGGAGGCATCATGA

Upstream 100 bases:

>100_bases
TAGCCTCTCTTGACCCGCATAACTTAAGAGCAAAGCAACTTAAGGACAATCCTCCAGGTGATAGAACCACTGCCGCCAAA
CTTGCAGAAGAGGTTGCCTA

Downstream 100 bases:

>100_bases
ACCAAGTTGTCGATGCTTCTAACACGTTTAATACAGTTTCTGATCTAAAAACAGATTTAACGGTAATGCTCGGTTGTCGT
TATCCGATTGTTCAAACGGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MPNNTAHSEAAAQTTANQLASLDGCHREDLDGVVFYEVREQVAIVTMNRPEYNNAQNSKMTYALDAAFKRACDDDNVKVI
ILAGEGKHFSAGHDIGTPGRDVDQEFDRASLWYDHSNKSGGEFLYAREQEVYLGMCRRWRDMPKPTIAMVQGACIAGGLM
LAWVCDLIICSDDAYYCDPVVRMGIPGVEYFAHVHELNPRIAKEFLFLGDKMNAQRAYELGMVNRIFARKDLTEETLTIA
NKIAQMPRLGLQLTKQAINNAEDLMGKRSTMDMVFGLHHFAHVHNESISGDKLGGFDAKAMAKANKAASVSDKRKEAS

Sequences:

>Translated_318_residues
MPNNTAHSEAAAQTTANQLASLDGCHREDLDGVVFYEVREQVAIVTMNRPEYNNAQNSKMTYALDAAFKRACDDDNVKVI
ILAGEGKHFSAGHDIGTPGRDVDQEFDRASLWYDHSNKSGGEFLYAREQEVYLGMCRRWRDMPKPTIAMVQGACIAGGLM
LAWVCDLIICSDDAYYCDPVVRMGIPGVEYFAHVHELNPRIAKEFLFLGDKMNAQRAYELGMVNRIFARKDLTEETLTIA
NKIAQMPRLGLQLTKQAINNAEDLMGKRSTMDMVFGLHHFAHVHNESISGDKLGGFDAKAMAKANKAASVSDKRKEAS
>Mature_317_residues
PNNTAHSEAAAQTTANQLASLDGCHREDLDGVVFYEVREQVAIVTMNRPEYNNAQNSKMTYALDAAFKRACDDDNVKVII
LAGEGKHFSAGHDIGTPGRDVDQEFDRASLWYDHSNKSGGEFLYAREQEVYLGMCRRWRDMPKPTIAMVQGACIAGGLML
AWVCDLIICSDDAYYCDPVVRMGIPGVEYFAHVHELNPRIAKEFLFLGDKMNAQRAYELGMVNRIFARKDLTEETLTIAN
KIAQMPRLGLQLTKQAINNAEDLMGKRSTMDMVFGLHHFAHVHNESISGDKLGGFDAKAMAKANKAASVSDKRKEAS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=215, Percent_Identity=29.3023255813954, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI194097323, Length=225, Percent_Identity=28.4444444444444, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI70995211, Length=195, Percent_Identity=29.7435897435897, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI45643119, Length=227, Percent_Identity=26.431718061674, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI260275230, Length=227, Percent_Identity=26.431718061674, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI260274832, Length=227, Percent_Identity=26.431718061674, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1787659, Length=228, Percent_Identity=26.7543859649123, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788597, Length=237, Percent_Identity=29.1139240506329, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI221142681, Length=251, Percent_Identity=26.2948207171315, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787660, Length=237, Percent_Identity=29.1139240506329, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082183, Length=249, Percent_Identity=26.1044176706827, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=224, Percent_Identity=29.0178571428571, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=233, Percent_Identity=28.755364806867, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=231, Percent_Identity=26.4069264069264, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=232, Percent_Identity=28.448275862069, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=220, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=27.6, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=27.6, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24650670, Length=264, Percent_Identity=28.030303030303, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19920382, Length=223, Percent_Identity=28.2511210762332, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357171, Length=222, Percent_Identity=27.4774774774775, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24654903, Length=217, Percent_Identity=21.6589861751152, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 35360; Mature: 35228

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNNTAHSEAAAQTTANQLASLDGCHREDLDGVVFYEVREQVAIVTMNRPEYNNAQNSKM
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCE
TYALDAAFKRACDDDNVKVIILAGEGKHFSAGHDIGTPGRDVDQEFDRASLWYDHSNKSG
EEHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCC
GEFLYAREQEVYLGMCRRWRDMPKPTIAMVQGACIAGGLMLAWVCDLIICSDDAYYCDPV
CCEEEECCHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHEECCCCEECCHH
VRMGIPGVEYFAHVHELNPRIAKEFLFLGDKMNAQRAYELGMVNRIFARKDLTEETLTIA
HHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
NKIAQMPRLGLQLTKQAINNAEDLMGKRSTMDMVFGLHHFAHVHNESISGDKLGGFDAKA
HHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
MAKANKAASVSDKRKEAS
HHHHCCCCCCCCHHHCCC
>Mature Secondary Structure 
PNNTAHSEAAAQTTANQLASLDGCHREDLDGVVFYEVREQVAIVTMNRPEYNNAQNSKM
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCE
TYALDAAFKRACDDDNVKVIILAGEGKHFSAGHDIGTPGRDVDQEFDRASLWYDHSNKSG
EEHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCC
GEFLYAREQEVYLGMCRRWRDMPKPTIAMVQGACIAGGLMLAWVCDLIICSDDAYYCDPV
CCEEEECCHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHEECCCCEECCHH
VRMGIPGVEYFAHVHELNPRIAKEFLFLGDKMNAQRAYELGMVNRIFARKDLTEETLTIA
HHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
NKIAQMPRLGLQLTKQAINNAEDLMGKRSTMDMVFGLHHFAHVHNESISGDKLGGFDAKA
HHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
MAKANKAASVSDKRKEAS
HHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA