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The map label for this gene is kstD [H]

Identifier: 167624021

GI number: 167624021

Start: 2524911

End: 2526680

Strand: Direct

Name: kstD [H]

Synonym: Shal_2097

Alternate gene names: 167624021

Gene position: 2524911-2526680 (Clockwise)

Preceding gene: 167624020

Following gene: 167624022

Centisome position: 48.31

GC content: 44.12

Gene sequence:

>1770_bases
ATGACATTCAGTTCTTTAAATAAACAAATAAGAGAGACCAAGAAGTCGTCAATTAATCAATCAAATTATGATGTTGTAAT
TGTTGGTTCTGGCGCAGGGGCTATGGCGTCATCAATAGTTGCTGCTGACCATGGCTTGTCGGTATTGGTTGTCGAAAAGT
CAGCCCATTATGGGGGAACATCGGCACTCTCTGGCGGGGGGATTTGGATCCCCAATAACGATCATTTTAAGGCTAAAGGT
GGTGAAGATAGCTACCAGTTAGCTTTTGATTACCTAAAAGCATCGACAGAGAACACAGTGTCAGAGCCAAGACTGCACGC
TTATTTGACTCAAGCTCCTGAAATGATCCGCTATTTGGAAAATAATAGCCGAGTGCGCTACGCCGTTGCAGAAAAATATC
CTGATTACTATCCACATTTAGAAGGGGCTTTGCCTGGCGGGCGAACACTGGATCCTGAAATATTTGATACAAAATGTATG
GGTAGTGAAGTCGATAACCAATTGAAAGCATCTGCATCTACTCTGTTAATGGGCAAAATAGCTTGGACGGCACGAGATGC
GCATAAAGCGATGGCAAGAGAAAGAGGTTGGCGCTTTTTACTGTTAAAGCTAATGCTCAGATATAAATTAGATTTTAGTC
AACGACGTAAAACAAAGCGCGATAGACGAGCGGCTTTAGGCAGTTCACTAGTGGCCTCACTTAGGCAGTCAATGACCGAC
CGTAATATTCCGCTTTGGCTCAATACCGACTTTACCGATATTGTATTAAATAATGAGAAGGTTTCGGGGATTGAAGTTGT
CCGCGAAGGTGAAAAACAAATAATTAATTCCGCTCTAGGCGTGATTTTTGCTAGTGGCGGTTTTGAGCAAAATCAGAAGC
TTAGAGAAAAGTATTTACCTGAACCGTCAAATGTTAAGTGGAGTGCAACGCCCCCTGGTCATAATACTGGTGCTGCACTA
GAGGCCGGCATTCGCGCTGGCGCAAAAACTGACATTATGGATTGGTGCTGGTGGTCCCCAACAGTGAATGTACCTAAAGA
AGATAAAGCGAGAGGCATTTTTGCTGAACGTGCTTTTCCCGGTGCAATCGTTGTCGATGGACTTGGCAAGCGTTTTGTTA
ATGAGGCCGCTCCGTACCTTGAGTTCGGTGACGCTATGTACCGCAACAATGCAGCTACTGGCGGTAAATCATTACCTGCC
TGGGTAATTTTCGATGCTAACTTTCGTTTTAATTATGCCATGGGCCCGTTTATGCCTGCGCAAATCCAGCCAGATAGTAG
ATTGCCTAAGGGGTGGTTAAACACACTCTATTGGAAGGCTGAAAACCTTAATGATCTGGCCAAACAGATCCAAATAGATC
CTGAAGGACTTTCACAAACCGTAGAGCGAATTAATAGATTTGCAAAAACGGGTGTTGATGAAGACTTCGGCCGAGGAGGT
AATGTTTTTGACCGTTATTATGGTGACACCAATGTTAAACCTAACCCTTGCATAGCACCTGTTGCTAAAGGTCCATTCTA
TGCAATGAGAATTGAAGCTGGTGATATCGGTACAAAAGGCGGTTTACTGACTAACGAACATGCACAGGTATTAAGTGATA
CGAATACTCCTATTGCTGGGTTATATGCCATTGGCAACTGTTCAGCATCGGTAATGGGCCGCAGTTATCCTGGAGCTGGG
GCTACGTTGGGACCTGCGATGACATTTGGATATATCGCAGCAAATCATATTGCAAAGGGCAGAGCTAATGGCAATCAGAC
TATTCTCTAA

Upstream 100 bases:

>100_bases
CAATTTTCTTTAGTGGTTTCTTAACCCGCTGATACTTCTTCCGTTACGATTTTTAGCCGGAAGTAATGGTATTCATGAGT
TCACAATAGCGAGATTGCAA

Downstream 100 bases:

>100_bases
CATTGAAGCATTTACGTCTCTGTTATTTTGTCATTCCGACGAAGCATCTTGTTTATATAGCTGTGATGCGTTGATTGAAG
GTTTGCGTACTTGGCCAAAA

Product: fumarate reductase/succinate dehydrogenase flavoprotein domain-containing protein

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MTFSSLNKQIRETKKSSINQSNYDVVIVGSGAGAMASSIVAADHGLSVLVVEKSAHYGGTSALSGGGIWIPNNDHFKAKG
GEDSYQLAFDYLKASTENTVSEPRLHAYLTQAPEMIRYLENNSRVRYAVAEKYPDYYPHLEGALPGGRTLDPEIFDTKCM
GSEVDNQLKASASTLLMGKIAWTARDAHKAMARERGWRFLLLKLMLRYKLDFSQRRKTKRDRRAALGSSLVASLRQSMTD
RNIPLWLNTDFTDIVLNNEKVSGIEVVREGEKQIINSALGVIFASGGFEQNQKLREKYLPEPSNVKWSATPPGHNTGAAL
EAGIRAGAKTDIMDWCWWSPTVNVPKEDKARGIFAERAFPGAIVVDGLGKRFVNEAAPYLEFGDAMYRNNAATGGKSLPA
WVIFDANFRFNYAMGPFMPAQIQPDSRLPKGWLNTLYWKAENLNDLAKQIQIDPEGLSQTVERINRFAKTGVDEDFGRGG
NVFDRYYGDTNVKPNPCIAPVAKGPFYAMRIEAGDIGTKGGLLTNEHAQVLSDTNTPIAGLYAIGNCSASVMGRSYPGAG
ATLGPAMTFGYIAANHIAKGRANGNQTIL

Sequences:

>Translated_589_residues
MTFSSLNKQIRETKKSSINQSNYDVVIVGSGAGAMASSIVAADHGLSVLVVEKSAHYGGTSALSGGGIWIPNNDHFKAKG
GEDSYQLAFDYLKASTENTVSEPRLHAYLTQAPEMIRYLENNSRVRYAVAEKYPDYYPHLEGALPGGRTLDPEIFDTKCM
GSEVDNQLKASASTLLMGKIAWTARDAHKAMARERGWRFLLLKLMLRYKLDFSQRRKTKRDRRAALGSSLVASLRQSMTD
RNIPLWLNTDFTDIVLNNEKVSGIEVVREGEKQIINSALGVIFASGGFEQNQKLREKYLPEPSNVKWSATPPGHNTGAAL
EAGIRAGAKTDIMDWCWWSPTVNVPKEDKARGIFAERAFPGAIVVDGLGKRFVNEAAPYLEFGDAMYRNNAATGGKSLPA
WVIFDANFRFNYAMGPFMPAQIQPDSRLPKGWLNTLYWKAENLNDLAKQIQIDPEGLSQTVERINRFAKTGVDEDFGRGG
NVFDRYYGDTNVKPNPCIAPVAKGPFYAMRIEAGDIGTKGGLLTNEHAQVLSDTNTPIAGLYAIGNCSASVMGRSYPGAG
ATLGPAMTFGYIAANHIAKGRANGNQTIL
>Mature_588_residues
TFSSLNKQIRETKKSSINQSNYDVVIVGSGAGAMASSIVAADHGLSVLVVEKSAHYGGTSALSGGGIWIPNNDHFKAKGG
EDSYQLAFDYLKASTENTVSEPRLHAYLTQAPEMIRYLENNSRVRYAVAEKYPDYYPHLEGALPGGRTLDPEIFDTKCMG
SEVDNQLKASASTLLMGKIAWTARDAHKAMARERGWRFLLLKLMLRYKLDFSQRRKTKRDRRAALGSSLVASLRQSMTDR
NIPLWLNTDFTDIVLNNEKVSGIEVVREGEKQIINSALGVIFASGGFEQNQKLREKYLPEPSNVKWSATPPGHNTGAALE
AGIRAGAKTDIMDWCWWSPTVNVPKEDKARGIFAERAFPGAIVVDGLGKRFVNEAAPYLEFGDAMYRNNAATGGKSLPAW
VIFDANFRFNYAMGPFMPAQIQPDSRLPKGWLNTLYWKAENLNDLAKQIQIDPEGLSQTVERINRFAKTGVDEDFGRGGN
VFDRYYGDTNVKPNPCIAPVAKGPFYAMRIEAGDIGTKGGLLTNEHAQVLSDTNTPIAGLYAIGNCSASVMGRSYPGAGA
TLGPAMTFGYIAANHIAKGRANGNQTIL

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 64505; Mature: 64374

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSSLNKQIRETKKSSINQSNYDVVIVGSGAGAMASSIVAADHGLSVLVVEKSAHYGGT
CCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCC
SALSGGGIWIPNNDHFKAKGGEDSYQLAFDYLKASTENTVSEPRLHAYLTQAPEMIRYLE
CCCCCCEEECCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
NNSRVRYAVAEKYPDYYPHLEGALPGGRTLDPEIFDTKCMGSEVDNQLKASASTLLMGKI
CCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHCCHHHHHHHHHHHHHHEEEHH
AWTARDAHKAMARERGWRFLLLKLMLRYKLDFSQRRKTKRDRRAALGSSLVASLRQSMTD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RNIPLWLNTDFTDIVLNNEKVSGIEVVREGEKQIINSALGVIFASGGFEQNQKLREKYLP
CCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCC
EPSNVKWSATPPGHNTGAALEAGIRAGAKTDIMDWCWWSPTVNVPKEDKARGIFAERAFP
CCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCHHCCEEHHCCCC
GAIVVDGLGKRFVNEAAPYLEFGDAMYRNNAATGGKSLPAWVIFDANFRFNYAMGPFMPA
CEEEECCHHHHHHHHCCCHHHHCHHHHCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCC
QIQPDSRLPKGWLNTLYWKAENLNDLAKQIQIDPEGLSQTVERINRFAKTGVDEDFGRGG
CCCCCCCCCHHHHHHHEEECCCHHHHHHHEECCHHHHHHHHHHHHHHHHCCCCHHHCCCC
NVFDRYYGDTNVKPNPCIAPVAKGPFYAMRIEAGDIGTKGGLLTNEHAQVLSDTNTPIAG
CCHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEECCHHHHHCCCCCCEEE
LYAIGNCSASVMGRSYPGAGATLGPAMTFGYIAANHIAKGRANGNQTIL
EEEECCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TFSSLNKQIRETKKSSINQSNYDVVIVGSGAGAMASSIVAADHGLSVLVVEKSAHYGGT
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCC
SALSGGGIWIPNNDHFKAKGGEDSYQLAFDYLKASTENTVSEPRLHAYLTQAPEMIRYLE
CCCCCCEEECCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
NNSRVRYAVAEKYPDYYPHLEGALPGGRTLDPEIFDTKCMGSEVDNQLKASASTLLMGKI
CCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHCCHHHHHHHHHHHHHHEEEHH
AWTARDAHKAMARERGWRFLLLKLMLRYKLDFSQRRKTKRDRRAALGSSLVASLRQSMTD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RNIPLWLNTDFTDIVLNNEKVSGIEVVREGEKQIINSALGVIFASGGFEQNQKLREKYLP
CCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCC
EPSNVKWSATPPGHNTGAALEAGIRAGAKTDIMDWCWWSPTVNVPKEDKARGIFAERAFP
CCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCHHCCEEHHCCCC
GAIVVDGLGKRFVNEAAPYLEFGDAMYRNNAATGGKSLPAWVIFDANFRFNYAMGPFMPA
CEEEECCHHHHHHHHCCCHHHHCHHHHCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCC
QIQPDSRLPKGWLNTLYWKAENLNDLAKQIQIDPEGLSQTVERINRFAKTGVDEDFGRGG
CCCCCCCCCHHHHHHHEEECCCHHHHHHHEECCHHHHHHHHHHHHHHHHCCCCHHHCCCC
NVFDRYYGDTNVKPNPCIAPVAKGPFYAMRIEAGDIGTKGGLLTNEHAQVLSDTNTPIAG
CCHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEECCHHHHHCCCCCCEEE
LYAIGNCSASVMGRSYPGAGATLGPAMTFGYIAANHIAKGRANGNQTIL
EEEECCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]