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The map label for this gene is ykvO [H]

Identifier: 167624010

GI number: 167624010

Start: 2511163

End: 2511942

Strand: Reverse

Name: ykvO [H]

Synonym: Shal_2086

Alternate gene names: 167624010

Gene position: 2511942-2511163 (Counterclockwise)

Preceding gene: 167624011

Following gene: 167624008

Centisome position: 48.06

GC content: 47.82

Gene sequence:

>780_bases
ATGCCTACTACTGCGGTAACAGGATCAGCTTCAGGCATTGGCGCTGCGGTTTGCGCTCAACTTGAAAAAGCAGGACACAC
CATTATCGGTATCGATCGCAAAACGGATGACAATTTCACAAGTATTGCCGCCGATCTATCCACAGCTGAAGGCCGTCAAA
ACGCCATAGACCTTGTATATCAACAGTCTAATGGGGTATTGGATGGATTAGTATGTTGCGCCGGTCTTGGCGTCACTGCG
CCTAACTGTGGACTTATCGTTGAAGTTAATTACTTTGGCGTCACGTCTTTAGTTACTGGCCTCTATGACGCACTACGTCA
AGGTGAGCAAGCTTCTGTCGTTGTTATCGGTTCTGTGGCTGCGAATCAACAAGTAGCAGCGCCGCATCCGATGGCGCTAG
CCATGCTTGATAGCGATGAGGCCCTAGCAACGAAGTTAGCTAATGAAGACCAACAAGCTCACATTGCTTATGCCGCGTCA
AAGTTTGCTGTAACCAGTTGGAGTCGTCAAACGGCTGTAAAATGGGGTGGCAACGGGATCCGTGTTAATGTCGTTGCACC
CGGTGCGGTAGAAACACCTTTACATCAAGCTTCTAAAGACGATGCGCGTTATGGAGAAGCCGTTCGTAATTTTGTCGCAC
CGATTGGTCGTAATAGCGAAGCTGAAGAAATCGCTTCTGTTGTGACGTTCTTACAATCAAATCAAGCAAGTTTTATTCAT
GGCAGTGTGCTCTTTGTCGATGGTGGTATGGATGCAATGATGCGAGCCAATTCGTTTTAG

Upstream 100 bases:

>100_bases
TAACTTATCACTCGGCATACTGGCTGTATCAAAATCTACCATCGCTTTAAAGTTTACTTTTTAAGCGCTAAGTATTGCTA
ACGATGAAAGGAGAAATAAT

Downstream 100 bases:

>100_bases
ACGCTTAACTGAATAATAGGAACCCTAGGGTTCAGTTCAGTAAACTCCAGATAGCAAGAAGCCCGCTATAAAATAGCGGG
CTTCTTTATGTGTGGCGAAG

Product: 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MPTTAVTGSASGIGAAVCAQLEKAGHTIIGIDRKTDDNFTSIAADLSTAEGRQNAIDLVYQQSNGVLDGLVCCAGLGVTA
PNCGLIVEVNYFGVTSLVTGLYDALRQGEQASVVVIGSVAANQQVAAPHPMALAMLDSDEALATKLANEDQQAHIAYAAS
KFAVTSWSRQTAVKWGGNGIRVNVVAPGAVETPLHQASKDDARYGEAVRNFVAPIGRNSEAEEIASVVTFLQSNQASFIH
GSVLFVDGGMDAMMRANSF

Sequences:

>Translated_259_residues
MPTTAVTGSASGIGAAVCAQLEKAGHTIIGIDRKTDDNFTSIAADLSTAEGRQNAIDLVYQQSNGVLDGLVCCAGLGVTA
PNCGLIVEVNYFGVTSLVTGLYDALRQGEQASVVVIGSVAANQQVAAPHPMALAMLDSDEALATKLANEDQQAHIAYAAS
KFAVTSWSRQTAVKWGGNGIRVNVVAPGAVETPLHQASKDDARYGEAVRNFVAPIGRNSEAEEIASVVTFLQSNQASFIH
GSVLFVDGGMDAMMRANSF
>Mature_258_residues
PTTAVTGSASGIGAAVCAQLEKAGHTIIGIDRKTDDNFTSIAADLSTAEGRQNAIDLVYQQSNGVLDGLVCCAGLGVTAP
NCGLIVEVNYFGVTSLVTGLYDALRQGEQASVVVIGSVAANQQVAAPHPMALAMLDSDEALATKLANEDQQAHIAYAASK
FAVTSWSRQTAVKWGGNGIRVNVVAPGAVETPLHQASKDDARYGEAVRNFVAPIGRNSEAEEIASVVTFLQSNQASFIHG
SVLFVDGGMDAMMRANSF

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Escherichia coli, GI1787335, Length=265, Percent_Identity=29.811320754717, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082100, Length=271, Percent_Identity=23.6162361623616, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI2367365, Length=265, Percent_Identity=27.1698113207547, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI2367175, Length=96, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=273, Percent_Identity=26.3736263736264, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17555706, Length=251, Percent_Identity=29.4820717131474, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25147288, Length=264, Percent_Identity=28.4090909090909, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=274, Percent_Identity=27.007299270073, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=280, Percent_Identity=28.2142857142857, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=279, Percent_Identity=27.9569892473118, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24643142, Length=259, Percent_Identity=27.7992277992278, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24639444, Length=267, Percent_Identity=27.7153558052434, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24644339, Length=277, Percent_Identity=29.2418772563177, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24644337, Length=102, Percent_Identity=39.2156862745098, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355319, Length=273, Percent_Identity=27.1062271062271, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28571526, Length=274, Percent_Identity=28.8321167883212, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI23397609, Length=266, Percent_Identity=27.4436090225564, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 26887; Mature: 26756

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTAVTGSASGIGAAVCAQLEKAGHTIIGIDRKTDDNFTSIAADLSTAEGRQNAIDLVY
CCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEE
QQSNGVLDGLVCCAGLGVTAPNCGLIVEVNYFGVTSLVTGLYDALRQGEQASVVVIGSVA
ECCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECC
ANQQVAAPHPMALAMLDSDEALATKLANEDQQAHIAYAASKFAVTSWSRQTAVKWGGNGI
CCCCCCCCCCEEEEEECCCHHHHHHHCCCCCHHEEEEHHHHHEEECCCCCEEEEECCCEE
RVNVVAPGAVETPLHQASKDDARYGEAVRNFVAPIGRNSEAEEIASVVTFLQSNQASFIH
EEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEE
GSVLFVDGGMDAMMRANSF
EEEEEEECCHHHHHHCCCC
>Mature Secondary Structure 
PTTAVTGSASGIGAAVCAQLEKAGHTIIGIDRKTDDNFTSIAADLSTAEGRQNAIDLVY
CCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEE
QQSNGVLDGLVCCAGLGVTAPNCGLIVEVNYFGVTSLVTGLYDALRQGEQASVVVIGSVA
ECCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECC
ANQQVAAPHPMALAMLDSDEALATKLANEDQQAHIAYAASKFAVTSWSRQTAVKWGGNGI
CCCCCCCCCCEEEEEECCCHHHHHHHCCCCCHHEEEEHHHHHEEECCCCCEEEEECCCEE
RVNVVAPGAVETPLHQASKDDARYGEAVRNFVAPIGRNSEAEEIASVVTFLQSNQASFIH
EEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEE
GSVLFVDGGMDAMMRANSF
EEEEEEECCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]