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The map label for this gene is mii [H]

Identifier: 167623893

GI number: 167623893

Start: 2362693

End: 2363928

Strand: Direct

Name: mii [H]

Synonym: Shal_1964

Alternate gene names: 167623893

Gene position: 2362693-2363928 (Clockwise)

Preceding gene: 167623892

Following gene: 167623894

Centisome position: 45.2

GC content: 51.86

Gene sequence:

>1236_bases
ATGACCACATCAACTGAATCAAAACCAAGCAAAGCATCTGCACCGCAAATCAAAATCGCTGCTACCTACATGCGTGGCGG
CACCAGTAAAGGGGTATTTTTTAACCTAATTGACTTGCCGAAAGAGGCGCAGCAAGCAGGCGCTGCCCGTGATGCGCTGC
TGCTTAGAGTGATTGGCAGCCCAGACCCTTACGGTAAACAAACCGACGGCATGGGCGGCGCAACATCTAGCACCAGTAAA
ACGGCGATCCTTGCCAAGAGTAACAAGGCCGATCATGATGTCGATTACCTGTTTGGCCAAGTCGCTATCGACAGGCCCTT
TGTCGATTGGAGTGGCAACTGCGGCAACTTAACCGCAGCGGTTGGTTCATTTGCCATTAGTAACGGTTTAGTCGATAACA
GCCGCATACCAGACAATGGCATTAGCGTAGTGCGTATTTGGCAAAAAAATATCGGTAAAACCATCATCGCCCACGTACCC
ATCACCAATGGTAAAGTACAAGAAACCGGTGATTTTGAGCTCGATGGTGTCACGTTCCCCGCAGCTGAGGTGCAAGTTGA
GTTTTTAGACCCAGCCGATGGCGATGGCGCCATGTTTCCAACAGGCAACTTGGTTGATGAATTAATCGTGCCAACCGATG
TTGTCCCGAGTGGCGTTTTACAAGCCACCATGATCAACGCAGGTATTCCCACCGTGTTTATCAATGCGGCAGAACTGGGT
TACACAGGCGCAGAGCTACAAGAAGCGATTAATGGCGATGCAAGCGTGCTTGCCATGTTTGAGGTTATTCGTGCCCACGG
CGCAGTGCAGATGGGGCTAATTAACCATATTGATGAGGCCGCAAACCGCCAACATACCCCAAAAGTGGCGATAGTTGCCG
CGCCGATTGATTACACCTCATCGAGTGGCAAGGTGATAGACAAAAACACTATCGACTTAAATGTACGCGCGCTGTCTATG
GGTAAACTGCACCATGCTATGATGGGCACCGCTGCCGTCGCTATTGGCACCGCCGCCGCTATTCCGGGCACACTAGTCAG
CTTGGCAGCAGGCGAGGGACTGAGTAATGCCGTTAACCCTGGCTTTGTCACCTTTGGTCATCCATCTGGCACACTTAAAG
TGGGCGCCGAGGCGAGCCAAGTCGATGGTCAGTGGCAAGTTAATAAGGCGGTGATGAGTCGCAGCGCCCGCGTACTCATG
GAAGGTTGGGTGAGAGTGCCTGCGGATACGTTTTAA

Upstream 100 bases:

>100_bases
CACCAAAGCTCATCGTTATAACGATATAAAACCTCACGCACAGGTTGTTTGATTTTACCTATTACTAAATGGCTCAAACA
GCATTAAAACGGTGTTAATT

Downstream 100 bases:

>100_bases
TCTTGCAAGGCAGCCAGCAATCAATCAAGCCATCTGTAGCGATCAACTCAATTTGGCCATGTTGAATCGAATGTTGAATC
GAATGTTGAATCCAATGCTG

Product: putative AcnD-accessory protein PrpF

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTTSTESKPSKASAPQIKIAATYMRGGTSKGVFFNLIDLPKEAQQAGAARDALLLRVIGSPDPYGKQTDGMGGATSSTSK
TAILAKSNKADHDVDYLFGQVAIDRPFVDWSGNCGNLTAAVGSFAISNGLVDNSRIPDNGISVVRIWQKNIGKTIIAHVP
ITNGKVQETGDFELDGVTFPAAEVQVEFLDPADGDGAMFPTGNLVDELIVPTDVVPSGVLQATMINAGIPTVFINAAELG
YTGAELQEAINGDASVLAMFEVIRAHGAVQMGLINHIDEAANRQHTPKVAIVAAPIDYTSSSGKVIDKNTIDLNVRALSM
GKLHHAMMGTAAVAIGTAAAIPGTLVSLAAGEGLSNAVNPGFVTFGHPSGTLKVGAEASQVDGQWQVNKAVMSRSARVLM
EGWVRVPADTF

Sequences:

>Translated_411_residues
MTTSTESKPSKASAPQIKIAATYMRGGTSKGVFFNLIDLPKEAQQAGAARDALLLRVIGSPDPYGKQTDGMGGATSSTSK
TAILAKSNKADHDVDYLFGQVAIDRPFVDWSGNCGNLTAAVGSFAISNGLVDNSRIPDNGISVVRIWQKNIGKTIIAHVP
ITNGKVQETGDFELDGVTFPAAEVQVEFLDPADGDGAMFPTGNLVDELIVPTDVVPSGVLQATMINAGIPTVFINAAELG
YTGAELQEAINGDASVLAMFEVIRAHGAVQMGLINHIDEAANRQHTPKVAIVAAPIDYTSSSGKVIDKNTIDLNVRALSM
GKLHHAMMGTAAVAIGTAAAIPGTLVSLAAGEGLSNAVNPGFVTFGHPSGTLKVGAEASQVDGQWQVNKAVMSRSARVLM
EGWVRVPADTF
>Mature_410_residues
TTSTESKPSKASAPQIKIAATYMRGGTSKGVFFNLIDLPKEAQQAGAARDALLLRVIGSPDPYGKQTDGMGGATSSTSKT
AILAKSNKADHDVDYLFGQVAIDRPFVDWSGNCGNLTAAVGSFAISNGLVDNSRIPDNGISVVRIWQKNIGKTIIAHVPI
TNGKVQETGDFELDGVTFPAAEVQVEFLDPADGDGAMFPTGNLVDELIVPTDVVPSGVLQATMINAGIPTVFINAAELGY
TGAELQEAINGDASVLAMFEVIRAHGAVQMGLINHIDEAANRQHTPKVAIVAAPIDYTSSSGKVIDKNTIDLNVRALSMG
KLHHAMMGTAAVAIGTAAAIPGTLVSLAAGEGLSNAVNPGFVTFGHPSGTLKVGAEASQVDGQWQVNKAVMSRSARVLME
GWVRVPADTF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=391, Percent_Identity=33.7595907928389, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 42779; Mature: 42648

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSTESKPSKASAPQIKIAATYMRGGTSKGVFFNLIDLPKEAQQAGAARDALLLRVIGS
CCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEECCCHHHHHCCCHHHEEEEEEECC
PDPYGKQTDGMGGATSSTSKTAILAKSNKADHDVDYLFGQVAIDRPFVDWSGNCGNLTAA
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHEEEECCCCCCCCCCCCCHHHH
VGSFAISNGLVDNSRIPDNGISVVRIWQKNIGKTIIAHVPITNGKVQETGDFELDGVTFP
HHHHHHHCCCCCCCCCCCCCCEEEEEEHHCCCCEEEEEECCCCCEECCCCCEEECCEECC
AAEVQVEFLDPADGDGAMFPTGNLVDELIVPTDVVPSGVLQATMINAGIPTVFINAAELG
CCEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEHHHCC
YTGAELQEAINGDASVLAMFEVIRAHGAVQMGLINHIDEAANRQHTPKVAIVAAPIDYTS
CCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCEEEEEEECCCCCC
SSGKVIDKNTIDLNVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVSLAAGEGLSNAVNP
CCCEEEECCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCC
GFVTFGHPSGTLKVGAEASQVDGQWQVNKAVMSRSARVLMEGWVRVPADTF
CEEEECCCCCCEEECCCHHHCCCCEEHHHHHHHHHHHHHHHCCEECCCCCC
>Mature Secondary Structure 
TTSTESKPSKASAPQIKIAATYMRGGTSKGVFFNLIDLPKEAQQAGAARDALLLRVIGS
CCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEECCCHHHHHCCCHHHEEEEEEECC
PDPYGKQTDGMGGATSSTSKTAILAKSNKADHDVDYLFGQVAIDRPFVDWSGNCGNLTAA
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHEEEECCCCCCCCCCCCCHHHH
VGSFAISNGLVDNSRIPDNGISVVRIWQKNIGKTIIAHVPITNGKVQETGDFELDGVTFP
HHHHHHHCCCCCCCCCCCCCCEEEEEEHHCCCCEEEEEECCCCCEECCCCCEEECCEECC
AAEVQVEFLDPADGDGAMFPTGNLVDELIVPTDVVPSGVLQATMINAGIPTVFINAAELG
CCEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEHHHCC
YTGAELQEAINGDASVLAMFEVIRAHGAVQMGLINHIDEAANRQHTPKVAIVAAPIDYTS
CCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCEEEEEEECCCCCC
SSGKVIDKNTIDLNVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVSLAAGEGLSNAVNP
CCCEEEECCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCC
GFVTFGHPSGTLKVGAEASQVDGQWQVNKAVMSRSARVLMEGWVRVPADTF
CEEEECCCCCCEEECCCHHHCCCCEEHHHHHHHHHHHHHHHCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA