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The map label for this gene is pykA [H]

Identifier: 167623877

GI number: 167623877

Start: 2339988

End: 2341424

Strand: Reverse

Name: pykA [H]

Synonym: Shal_1947

Alternate gene names: 167623877

Gene position: 2341424-2339988 (Counterclockwise)

Preceding gene: 167623878

Following gene: 167623874

Centisome position: 44.8

GC content: 45.93

Gene sequence:

>1437_bases
ATGTTCCGCAGAACCAAAATTGTAACAACTTTGGGTCCAGCCACCGACCGTGATGACAACTTACGCCGCATTATTGCTGC
CGGCGCTAACGTTGTTCGCTTAAACTTCTCTCACGGTGCACCAGAAGATCATAAGAAACGCGCAAATGATACACGAGCGA
TTGCAAAAGAGCTTGGCGTTCATGTTGCAGTTTTAGGCGATCTTCAAGGTCCCAAAATTCGTATCTCAACTTTCAAAGAC
AACAAAAAGGTCATGTTAGAGCTTGGTCAAGCCTATACCCTTGATGGCGATCTTGCGAAAGGCGAAGGTGACGAGACACA
AGTCGGTATCGATTATAAAGAGTTACCACAAGACGTGAGTCTTGGTGATATCTTAATGCTCGATGATGGCCGCGTTCAGT
TGCGCGTTGAAAGAGTTGAAGGTAACAAGGTTCACACCAGCGTTACTGTCGCAGGTCCTTTATCTAATAATAAAGGGATC
AACAAGCAAGGCGGTGGCTTATCGGCTGCGGCACTGACTGAAAAAGATAAACGAGATATCGTTACTGCTGCAGAGATTGG
CGTTGATTACCTTGCGGTATCATTCCCAAGAAGTGGTGCTGATCTAAACTATGCTCGCGACTTAGCGCGTAAAGCGGGTA
GCAACGCATTGATTGTTTCTAAAGTCGAGCGTGCAGAAGCCGTAGCCACCGACGAAGCAATGGATGATGTGATCATCGCC
TCTGATGCCGTTATGGTTGCCCGTGGTGACTTAGGTGTTGAAATTGGCGACCCAGCTCTTGTTGCTGTGCAAAAACGACT
AATTAGCCGTTCTCGTCAATTGAATAAGCCTGTCATTACAGCAACTCAAATGATGGAATCAATGATCTCTAGCCCAATGC
CAACTCGTGCAGAGGTTATGGACGTTGCAAACGCAGTATTAGATGGCACAGATGCAGTAATGCTTAGCGCCGAAACCGCT
GCTGGTGACTTCCCTGAAGAAACCGTAAAAGCAATGGCAGAAGTATGTTTAGGTGCTGAATCACACCCAAGTGTTCAGGT
TTCTAAGCACAGATTAGATCAGCAATTCACGACTATCGAAGAAACGATTGCATTATCGACTATGTATGCCGCTAACCACC
TTGAAGGTATTAAGGCGATTGTGGCGTTAACAGAATCGGGTGCAACACCACAGATGATGTCACGCATTAGCTCTGCACTA
CCTATTTTTGCCATGTCTCGTCATGAGTCAACATTAGCAAAAATGGCACTTTATCGCGGGGTTCAACCAGTTTATTTTGA
TTCAACGACTCATGCACCTGAAGCTGTCGCTCAAAAAGCACTAGAGACTCTGTCTCAAGCGGGTTACCTTGTAAAAGGTG
ATATGGCGATGATGACTAAGGGTGATGCAATGGAAACTGTTGGTGGCACTAACACTAGCAAGGTTGTTGTTGTCTAA

Upstream 100 bases:

>100_bases
AAAACTTGAGTTAGATCATTTTGTTCGTAATTTTACTACACTAACGGGTATTGTCTGGTAATATAGAATCAGAATTTTTT
AAAACTTAACGGAGTATGTC

Downstream 100 bases:

>100_bases
CTCTAAGTGGTTGGTTAGCTAATAAAAAAGGTACGCAATTGCGTACCTTTTTTTATACTTCGTGATTCATACTAATTGGT
ATAATACCAATCAGTATAAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGAPEDHKKRANDTRAIAKELGVHVAVLGDLQGPKIRISTFKD
NKKVMLELGQAYTLDGDLAKGEGDETQVGIDYKELPQDVSLGDILMLDDGRVQLRVERVEGNKVHTSVTVAGPLSNNKGI
NKQGGGLSAAALTEKDKRDIVTAAEIGVDYLAVSFPRSGADLNYARDLARKAGSNALIVSKVERAEAVATDEAMDDVIIA
SDAVMVARGDLGVEIGDPALVAVQKRLISRSRQLNKPVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVKAMAEVCLGAESHPSVQVSKHRLDQQFTTIEETIALSTMYAANHLEGIKAIVALTESGATPQMMSRISSAL
PIFAMSRHESTLAKMALYRGVQPVYFDSTTHAPEAVAQKALETLSQAGYLVKGDMAMMTKGDAMETVGGTNTSKVVVV

Sequences:

>Translated_478_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGAPEDHKKRANDTRAIAKELGVHVAVLGDLQGPKIRISTFKD
NKKVMLELGQAYTLDGDLAKGEGDETQVGIDYKELPQDVSLGDILMLDDGRVQLRVERVEGNKVHTSVTVAGPLSNNKGI
NKQGGGLSAAALTEKDKRDIVTAAEIGVDYLAVSFPRSGADLNYARDLARKAGSNALIVSKVERAEAVATDEAMDDVIIA
SDAVMVARGDLGVEIGDPALVAVQKRLISRSRQLNKPVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVKAMAEVCLGAESHPSVQVSKHRLDQQFTTIEETIALSTMYAANHLEGIKAIVALTESGATPQMMSRISSAL
PIFAMSRHESTLAKMALYRGVQPVYFDSTTHAPEAVAQKALETLSQAGYLVKGDMAMMTKGDAMETVGGTNTSKVVVV
>Mature_478_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGAPEDHKKRANDTRAIAKELGVHVAVLGDLQGPKIRISTFKD
NKKVMLELGQAYTLDGDLAKGEGDETQVGIDYKELPQDVSLGDILMLDDGRVQLRVERVEGNKVHTSVTVAGPLSNNKGI
NKQGGGLSAAALTEKDKRDIVTAAEIGVDYLAVSFPRSGADLNYARDLARKAGSNALIVSKVERAEAVATDEAMDDVIIA
SDAVMVARGDLGVEIGDPALVAVQKRLISRSRQLNKPVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVKAMAEVCLGAESHPSVQVSKHRLDQQFTTIEETIALSTMYAANHLEGIKAIVALTESGATPQMMSRISSAL
PIFAMSRHESTLAKMALYRGVQPVYFDSTTHAPEAVAQKALETLSQAGYLVKGDMAMMTKGDAMETVGGTNTSKVVVV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=495, Percent_Identity=35.5555555555556, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI33286420, Length=495, Percent_Identity=35.5555555555556, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=35.5555555555556, Blast_Score=258, Evalue=9e-69,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=33.6693548387097, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=33.6693548387097, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=34.9480968858132, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=34.9480968858132, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=32.7659574468085, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=32.7659574468085, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=32.2429906542056, Blast_Score=102, Evalue=9e-22,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=70.8595387840671, Blast_Score=680, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=480, Percent_Identity=37.5, Blast_Score=286, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=36.1396303901437, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=493, Percent_Identity=35.4969574036511, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI17506831, Length=493, Percent_Identity=35.4969574036511, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI71984413, Length=493, Percent_Identity=35.4969574036511, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI17506829, Length=493, Percent_Identity=35.4969574036511, Blast_Score=238, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6319279, Length=474, Percent_Identity=36.0759493670886, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=433, Percent_Identity=33.9491916859122, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24648964, Length=498, Percent_Identity=33.9357429718875, Blast_Score=244, Evalue=9e-65,
Organism=Drosophila melanogaster, GI28571814, Length=498, Percent_Identity=33.9357429718875, Blast_Score=244, Evalue=9e-65,
Organism=Drosophila melanogaster, GI24648966, Length=449, Percent_Identity=34.2984409799555, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24581235, Length=496, Percent_Identity=30.8467741935484, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24646914, Length=316, Percent_Identity=34.1772151898734, Blast_Score=154, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51045; Mature: 51045

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGAPEDHKKRANDTRAIAKELGV
CCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHCHHHHHHHHHHCE
HVAVLGDLQGPKIRISTFKDNKKVMLELGQAYTLDGDLAKGEGDETQVGIDYKELPQDVS
EEEEEECCCCCEEEEEEECCCCEEEEEECCEEEECCCCCCCCCCCCEECCCHHHCCCCCC
LGDILMLDDGRVQLRVERVEGNKVHTSVTVAGPLSNNKGINKQGGGLSAAALTEKDKRDI
CCCEEEEECCEEEEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCEEEHHHCCCHHHHH
VTAAEIGVDYLAVSFPRSGADLNYARDLARKAGSNALIVSKVERAEAVATDEAMDDVIIA
HHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCEEEE
SDAVMVARGDLGVEIGDPALVAVQKRLISRSRQLNKPVITATQMMESMISSPMPTRAEVM
CCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHH
DVANAVLDGTDAVMLSAETAAGDFPEETVKAMAEVCLGAESHPSVQVSKHRLDQQFTTIE
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHH
ETIALSTMYAANHLEGIKAIVALTESGATPQMMSRISSALPIFAMSRHESTLAKMALYRG
HHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHC
VQPVYFDSTTHAPEAVAQKALETLSQAGYLVKGDMAMMTKGDAMETVGGTNTSKVVVV
CCCEEECCCCCCHHHHHHHHHHHHHHCCCEEECCEEEEECCCCHHHCCCCCCCEEEEC
>Mature Secondary Structure
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGAPEDHKKRANDTRAIAKELGV
CCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHCHHHHHHHHHHCE
HVAVLGDLQGPKIRISTFKDNKKVMLELGQAYTLDGDLAKGEGDETQVGIDYKELPQDVS
EEEEEECCCCCEEEEEEECCCCEEEEEECCEEEECCCCCCCCCCCCEECCCHHHCCCCCC
LGDILMLDDGRVQLRVERVEGNKVHTSVTVAGPLSNNKGINKQGGGLSAAALTEKDKRDI
CCCEEEEECCEEEEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCEEEHHHCCCHHHHH
VTAAEIGVDYLAVSFPRSGADLNYARDLARKAGSNALIVSKVERAEAVATDEAMDDVIIA
HHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCEEEE
SDAVMVARGDLGVEIGDPALVAVQKRLISRSRQLNKPVITATQMMESMISSPMPTRAEVM
CCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHH
DVANAVLDGTDAVMLSAETAAGDFPEETVKAMAEVCLGAESHPSVQVSKHRLDQQFTTIE
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHH
ETIALSTMYAANHLEGIKAIVALTESGATPQMMSRISSALPIFAMSRHESTLAKMALYRG
HHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHC
VQPVYFDSTTHAPEAVAQKALETLSQAGYLVKGDMAMMTKGDAMETVGGTNTSKVVVV
CCCEEECCCCCCHHHHHHHHHHHHHHCCCEEECCEEEEECCCCHHHCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]