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The map label for this gene is gacS [H]

Identifier: 167623839

GI number: 167623839

Start: 2291392

End: 2293848

Strand: Direct

Name: gacS [H]

Synonym: Shal_1909

Alternate gene names: 167623839

Gene position: 2291392-2293848 (Clockwise)

Preceding gene: 167623838

Following gene: 167623847

Centisome position: 43.84

GC content: 36.67

Gene sequence:

>2457_bases
ATGGCCTATTGTTTATGGGCTGGTGCAACAGGAGGTCGCATGCTTAAACAGGGAAGTTGGTCTATAAAGAGTTTTTTGCT
AGCAGGAAGCGTAGCAATTGTTTTATTGCTTATTATTAATGTATCGATAACCCAGTATGTGTCGAATACTAAATTGGCAT
TATCACAGCTTGAAAATGAGGTTGTTGTCTCTGCCAACACATTATTGATGATACGGCGCAACGAGAAGGACTTTATTAGT
CGAATAGAACCAAAATATACTTTTCAAGTACTGGACTTTAAGGCAAAGCTGGTACAGCAATTAACTCAGATTGATAGGTT
ACTGACTCAAAGTAATTTAGATATAAACTATGACAATCAACGGGTTTTGGAACAGCTTGATGATTATATTGATACTTTTT
CCCTTCTAATTAATGAAATATACAGTATCAATGGCACCCGTGCTGAACAAGGACTACTTGCACGATTTGAACGTAACTCG
AATGAACTCCAATTACTCACGAATCAGTTGAAAGATACAGAATTATTGGAGCTTATAATCGCCAATCAGTTAACGGTATT
TGATTTTTTTAAAAGTTTCGACTCCGCTTTACTCACTAGAGTTACTCATCAGTTTAAAATAATAGAACAGCATATTAAAG
ATAAGCCTCAATCTACATTACCGTTGCAGCAAGCTTTTTTGGCATTTGAACATGCTTTTTTTCAGTTGCAGGCATCTATG
AAAAGGAGAGGTTACTCGGAGTCGTTAGGCTATCATGGAACACTTAGACAAAATGTTCACAGTGTGGAACGCAGTCTGGA
AACTCTGTTTAATACGATCCCACTTAAAGTTGATAAAAGTGTTTCAGGGTTAGTCATCACCCAATCTATTGTTGAGATCT
TATTAGTGCTCTTAATTGTTGGAATACTATATTATATTTTTTATTCCATCACTAGGATGGAGCAAGGGCTTATTTTCGCA
AGAGAAAAAGAGGAACAAGCGAACAGTGCTAAAAGCTCATTTTTGGCTAATATGTCTCATGAAATCAGGACGCCACTTAA
TGGCATCATAGGTATGACAGAGATTTTAACTGACTCAAAACTGACCGCGATACAGAAAGATTACCTTGCAACAATTAACT
CCTCTTCTCAAACCTTATTAATGTTAATAAATGATATCTTAGATTTATCAAAAATAGAGTCTGGCCATCTTGAGATCTGT
CCACATACTTGCAATATAAAAGAGGTTATATATGACACCGTAGCTTTAATCGCTCCTAAAGCCCAACAAAGAAGCATAGA
GATGAGAATAACCATTGACCAAAATATTCCATGCTATGTTAGGGCTGACGAACAAAAACTGCGACAGGTTTTAATGAATC
TAGCGTCAAATGCGGTTAAGTTTACCCATTCAGGTTCCGTTTCGTTCAAGTTACAGCTAGAAGTTGAGACGACTGATACC
ATATGCATACTGTTTTCTGTCAAAGATACTGGCATCGGGATCGATGAAAATAAACATATGCAAGTATTTGAAGAGTTTCA
ACAAGAGGAAAGAAATACTTCGGTCGAGTATGGTGGAACAGGACTTGGTTTATCTATTTCAACCAAGTTGGTAAGGATGA
TGGGGGGCGAAATAGCGATAAATTCTTCAAAGGGACTAGGCAGTGAATTCTATTTTTATTTAACCTTTGATAGGCAGGAG
CATATTGCAAAACTATCTAGTTCTCAGCACTTAATCTATTGCGCTAAATTGCAGAATCCATTTTTAGTTCAAAATATTAA
AAGTTTGCATCACCAACTTGATGATACGGATAGTGTGGATGAAATTATCCCAATGCTGAGGCAATCAAGTATCATAATTT
TTAATGCTGAAGACATTAAAGATAAACAGATGTTAGCAGGTATAGCATTAAAACTTTCTACCCCCCCCATTCTCATTGCA
AGCCAAATTGACTCGGTTAAAGAAGATTATGGTGATTTGGTTGCGGGATATATCACACTACCATTGTTAGGTAAGCGCTT
AGAGACTTTAATTAACTCAGTGTCTAACCGCTACGAGCAAAAAGAGAGTTTTGTACCAGTAGAGCTTGAGCTAAACAAGA
ATAAAAAAATAGTGCTGGTGGTTGAAGACAATAAGGTTAATCAGCAGGTAGTTTCAATTAATTTAAATAAGCTAAACCTT
CCATATCTTATTGCCAATGACGGAAGAGAAGCATTAGAACATTATAAGCGTCATATCGGTAACTTCTCTGTGATTTTAAT
GGATTGCATGATGCCCGTAATGGATGGATTTGAAGCGACAAGAGCAATACGCGAATTTGAAAAAGAGGAAGATGCTAAGC
GTGTTCAAATTATAGCGTTAACGGCGTCAATTCTTGATGATGATATACAAAAGTGTTTTGACTCAGGTATGGATGACTAT
TTACCGAAGCCCTTCAAGCGTGAAGTGTTAGTCGATAAACTCGCTAAGTTGCATTAA

Upstream 100 bases:

>100_bases
TAAAAAGTATTCGTCACTCGTTAAAAGTACATTTTATAGACTAATATCCCTTATCTTTTTGATTCATTTAACAAGTTATT
TATTGGTGCTAACTGTTTAT

Downstream 100 bases:

>100_bases
GACACCGTGCTATTGAGATTAGCCTAGCATTTCAATGTGTCTTTATGTGGCTTGTGTAAACGATTACGCTGTATGCGCCA
CTGCTCCAATGTCGATTTTA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 818; Mature: 817

Protein sequence:

>818_residues
MAYCLWAGATGGRMLKQGSWSIKSFLLAGSVAIVLLLIINVSITQYVSNTKLALSQLENEVVVSANTLLMIRRNEKDFIS
RIEPKYTFQVLDFKAKLVQQLTQIDRLLTQSNLDINYDNQRVLEQLDDYIDTFSLLINEIYSINGTRAEQGLLARFERNS
NELQLLTNQLKDTELLELIIANQLTVFDFFKSFDSALLTRVTHQFKIIEQHIKDKPQSTLPLQQAFLAFEHAFFQLQASM
KRRGYSESLGYHGTLRQNVHSVERSLETLFNTIPLKVDKSVSGLVITQSIVEILLVLLIVGILYYIFYSITRMEQGLIFA
REKEEQANSAKSSFLANMSHEIRTPLNGIIGMTEILTDSKLTAIQKDYLATINSSSQTLLMLINDILDLSKIESGHLEIC
PHTCNIKEVIYDTVALIAPKAQQRSIEMRITIDQNIPCYVRADEQKLRQVLMNLASNAVKFTHSGSVSFKLQLEVETTDT
ICILFSVKDTGIGIDENKHMQVFEEFQQEERNTSVEYGGTGLGLSISTKLVRMMGGEIAINSSKGLGSEFYFYLTFDRQE
HIAKLSSSQHLIYCAKLQNPFLVQNIKSLHHQLDDTDSVDEIIPMLRQSSIIIFNAEDIKDKQMLAGIALKLSTPPILIA
SQIDSVKEDYGDLVAGYITLPLLGKRLETLINSVSNRYEQKESFVPVELELNKNKKIVLVVEDNKVNQQVVSINLNKLNL
PYLIANDGREALEHYKRHIGNFSVILMDCMMPVMDGFEATRAIREFEKEEDAKRVQIIALTASILDDDIQKCFDSGMDDY
LPKPFKREVLVDKLAKLH

Sequences:

>Translated_818_residues
MAYCLWAGATGGRMLKQGSWSIKSFLLAGSVAIVLLLIINVSITQYVSNTKLALSQLENEVVVSANTLLMIRRNEKDFIS
RIEPKYTFQVLDFKAKLVQQLTQIDRLLTQSNLDINYDNQRVLEQLDDYIDTFSLLINEIYSINGTRAEQGLLARFERNS
NELQLLTNQLKDTELLELIIANQLTVFDFFKSFDSALLTRVTHQFKIIEQHIKDKPQSTLPLQQAFLAFEHAFFQLQASM
KRRGYSESLGYHGTLRQNVHSVERSLETLFNTIPLKVDKSVSGLVITQSIVEILLVLLIVGILYYIFYSITRMEQGLIFA
REKEEQANSAKSSFLANMSHEIRTPLNGIIGMTEILTDSKLTAIQKDYLATINSSSQTLLMLINDILDLSKIESGHLEIC
PHTCNIKEVIYDTVALIAPKAQQRSIEMRITIDQNIPCYVRADEQKLRQVLMNLASNAVKFTHSGSVSFKLQLEVETTDT
ICILFSVKDTGIGIDENKHMQVFEEFQQEERNTSVEYGGTGLGLSISTKLVRMMGGEIAINSSKGLGSEFYFYLTFDRQE
HIAKLSSSQHLIYCAKLQNPFLVQNIKSLHHQLDDTDSVDEIIPMLRQSSIIIFNAEDIKDKQMLAGIALKLSTPPILIA
SQIDSVKEDYGDLVAGYITLPLLGKRLETLINSVSNRYEQKESFVPVELELNKNKKIVLVVEDNKVNQQVVSINLNKLNL
PYLIANDGREALEHYKRHIGNFSVILMDCMMPVMDGFEATRAIREFEKEEDAKRVQIIALTASILDDDIQKCFDSGMDDY
LPKPFKREVLVDKLAKLH
>Mature_817_residues
AYCLWAGATGGRMLKQGSWSIKSFLLAGSVAIVLLLIINVSITQYVSNTKLALSQLENEVVVSANTLLMIRRNEKDFISR
IEPKYTFQVLDFKAKLVQQLTQIDRLLTQSNLDINYDNQRVLEQLDDYIDTFSLLINEIYSINGTRAEQGLLARFERNSN
ELQLLTNQLKDTELLELIIANQLTVFDFFKSFDSALLTRVTHQFKIIEQHIKDKPQSTLPLQQAFLAFEHAFFQLQASMK
RRGYSESLGYHGTLRQNVHSVERSLETLFNTIPLKVDKSVSGLVITQSIVEILLVLLIVGILYYIFYSITRMEQGLIFAR
EKEEQANSAKSSFLANMSHEIRTPLNGIIGMTEILTDSKLTAIQKDYLATINSSSQTLLMLINDILDLSKIESGHLEICP
HTCNIKEVIYDTVALIAPKAQQRSIEMRITIDQNIPCYVRADEQKLRQVLMNLASNAVKFTHSGSVSFKLQLEVETTDTI
CILFSVKDTGIGIDENKHMQVFEEFQQEERNTSVEYGGTGLGLSISTKLVRMMGGEIAINSSKGLGSEFYFYLTFDRQEH
IAKLSSSQHLIYCAKLQNPFLVQNIKSLHHQLDDTDSVDEIIPMLRQSSIIIFNAEDIKDKQMLAGIALKLSTPPILIAS
QIDSVKEDYGDLVAGYITLPLLGKRLETLINSVSNRYEQKESFVPVELELNKNKKIVLVVEDNKVNQQVVSINLNKLNLP
YLIANDGREALEHYKRHIGNFSVILMDCMMPVMDGFEATRAIREFEKEEDAKRVQIIALTASILDDDIQKCFDSGMDDYL
PKPFKREVLVDKLAKLH

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=516, Percent_Identity=29.8449612403101, Blast_Score=229, Evalue=6e-61,
Organism=Escherichia coli, GI145693157, Length=530, Percent_Identity=29.622641509434, Blast_Score=200, Evalue=3e-52,
Organism=Escherichia coli, GI87081816, Length=239, Percent_Identity=38.4937238493724, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI48994928, Length=227, Percent_Identity=37.0044052863436, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788713, Length=248, Percent_Identity=32.258064516129, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1786600, Length=246, Percent_Identity=30.0813008130081, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790436, Length=287, Percent_Identity=26.1324041811847, Blast_Score=87, Evalue=6e-18,
Organism=Escherichia coli, GI1786912, Length=303, Percent_Identity=25.7425742574257, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87082128, Length=242, Percent_Identity=29.3388429752066, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1788549, Length=229, Percent_Identity=24.4541484716157, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1788393, Length=235, Percent_Identity=24.6808510638298, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1786783, Length=234, Percent_Identity=26.0683760683761, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790346, Length=233, Percent_Identity=23.6051502145923, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322044, Length=394, Percent_Identity=28.4263959390863, Blast_Score=121, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6323034, Length=143, Percent_Identity=34.965034965035, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322000, Length=116, Percent_Identity=37.0689655172414, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 92757; Mature: 92626

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYCLWAGATGGRMLKQGSWSIKSFLLAGSVAIVLLLIINVSITQYVSNTKLALSQLENE
CEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VVVSANTLLMIRRNEKDFISRIEPKYTFQVLDFKAKLVQQLTQIDRLLTQSNLDINYDNQ
EEEEECEEEEEECCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
RVLEQLDDYIDTFSLLINEIYSINGTRAEQGLLARFERNSNELQLLTNQLKDTELLELII
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
ANQLTVFDFFKSFDSALLTRVTHQFKIIEQHIKDKPQSTLPLQQAFLAFEHAFFQLQASM
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KRRGYSESLGYHGTLRQNVHSVERSLETLFNTIPLKVDKSVSGLVITQSIVEILLVLLIV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHH
GILYYIFYSITRMEQGLIFAREKEEQANSAKSSFLANMSHEIRTPLNGIIGMTEILTDSK
HHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCH
LTAIQKDYLATINSSSQTLLMLINDILDLSKIESGHLEICPHTCNIKEVIYDTVALIAPK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCC
AQQRSIEMRITIDQNIPCYVRADEQKLRQVLMNLASNAVKFTHSGSVSFKLQLEVETTDT
CHHCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHCCEEEEECCCEEEEEEEEEECCCE
ICILFSVKDTGIGIDENKHMQVFEEFQQEERNTSVEYGGTGLGLSISTKLVRMMGGEIAI
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCEEEE
NSSKGLGSEFYFYLTFDRQEHIAKLSSSQHLIYCAKLQNPFLVQNIKSLHHQLDDTDSVD
ECCCCCCCEEEEEEEECCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHH
EIIPMLRQSSIIIFNAEDIKDKQMLAGIALKLSTPPILIASQIDSVKEDYGDLVAGYITL
HHHHHHHCCCEEEEECCCCCHHHHHHHEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHH
PLLGKRLETLINSVSNRYEQKESFVPVELELNKNKKIVLVVEDNKVNQQVVSINLNKLNL
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCEEEEEEECEECC
PYLIANDGREALEHYKRHIGNFSVILMDCMMPVMDGFEATRAIREFEKEEDAKRVQIIAL
CEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEE
TASILDDDIQKCFDSGMDDYLPKPFKREVLVDKLAKLH
HHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AYCLWAGATGGRMLKQGSWSIKSFLLAGSVAIVLLLIINVSITQYVSNTKLALSQLENE
EEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VVVSANTLLMIRRNEKDFISRIEPKYTFQVLDFKAKLVQQLTQIDRLLTQSNLDINYDNQ
EEEEECEEEEEECCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
RVLEQLDDYIDTFSLLINEIYSINGTRAEQGLLARFERNSNELQLLTNQLKDTELLELII
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
ANQLTVFDFFKSFDSALLTRVTHQFKIIEQHIKDKPQSTLPLQQAFLAFEHAFFQLQASM
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KRRGYSESLGYHGTLRQNVHSVERSLETLFNTIPLKVDKSVSGLVITQSIVEILLVLLIV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHH
GILYYIFYSITRMEQGLIFAREKEEQANSAKSSFLANMSHEIRTPLNGIIGMTEILTDSK
HHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCH
LTAIQKDYLATINSSSQTLLMLINDILDLSKIESGHLEICPHTCNIKEVIYDTVALIAPK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCC
AQQRSIEMRITIDQNIPCYVRADEQKLRQVLMNLASNAVKFTHSGSVSFKLQLEVETTDT
CHHCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHCCEEEEECCCEEEEEEEEEECCCE
ICILFSVKDTGIGIDENKHMQVFEEFQQEERNTSVEYGGTGLGLSISTKLVRMMGGEIAI
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCEEEE
NSSKGLGSEFYFYLTFDRQEHIAKLSSSQHLIYCAKLQNPFLVQNIKSLHHQLDDTDSVD
ECCCCCCCEEEEEEEECCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHH
EIIPMLRQSSIIIFNAEDIKDKQMLAGIALKLSTPPILIASQIDSVKEDYGDLVAGYITL
HHHHHHHCCCEEEEECCCCCHHHHHHHEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHH
PLLGKRLETLINSVSNRYEQKESFVPVELELNKNKKIVLVVEDNKVNQQVVSINLNKLNL
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCEEEEEEECEECC
PYLIANDGREALEHYKRHIGNFSVILMDCMMPVMDGFEATRAIREFEKEEDAKRVQIIAL
CEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEE
TASILDDDIQKCFDSGMDDYLPKPFKREVLVDKLAKLH
HHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]