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The map label for this gene is rluE [H]

Identifier: 167623648

GI number: 167623648

Start: 2060242

End: 2060961

Strand: Reverse

Name: rluE [H]

Synonym: Shal_1717

Alternate gene names: 167623648

Gene position: 2060961-2060242 (Counterclockwise)

Preceding gene: 167623650

Following gene: 167623647

Centisome position: 39.43

GC content: 44.31

Gene sequence:

>720_bases
GTGACACAGCCATTTTCAAAAAATAGAACGACCAATAAAAATAACAAGACTAGCTCTTCTGGCTTTGCTAGGAACGGTAG
CAACAAGCCTGCTACCGTTGCTAAGACCCCAAATAAAAACGACAAACCTCACAATAGAGCCATTAGCAAAGCTAGACCTA
AGGCTAAAACTGTTGCCAATCCGGTGATAGTACTATTTAACAAACCCTTCGATGTACTGTGTCAGTTTACCGATGAGCAG
GGCCGAAAAACCTTAAAAGACTATATCCCGGTGCCTGGCGTGTATGCTGCGGGACGACTCGATAGAGACAGCGAAGGCCT
GTTATTACTGACCAATGACGGTAAATTACAATCGCAAATCACAGAACCTAAAAAGAAAACCTATAAAACCTATTGGGCCC
AAGTTGAAGGTGCGCCTGATGACAGTGATCTCGAAAAACTGCGCCAAGGCGTTGAACTGAAAGATGGCATGACGTTACCC
GCTAAAATTAGCATCATGGATAACCCTGCAATATGGCCAAGAACGCCGCCGATCCGTGAGAGAGCGAATATCCCTACCAC
TTGGTTAGAGATCCAAATATGTGAAGGCCGTAACCGCCAGGTTAGACGCATGACAGCCCATATTGGCTTCCCGACGTTAA
GATTAATCCGCTATAAAATAGGTCAATGGAGTCTAGATGACCTACAAAACGGTGAATATCGTCAACTTGATATGAAATAA

Upstream 100 bases:

>100_bases
GTCGACATCATAAAACATTCCACGCAATATTCAAATTCCACTAATAGCCAATGCACAGTACAATAGATAAATATCATTCC
AAAATGAAAAGTATCGCAGC

Downstream 100 bases:

>100_bases
CCATCAATGTGAGTGACAAGCAATGACTGAGCGCTATAAACCCAATACCACTGTTGCGTGTGTTATCGAAGCGCAGGGGA
AATTCCTACTCGTTGAAGAA

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTQPFSKNRTTNKNNKTSSSGFARNGSNKPATVAKTPNKNDKPHNRAISKARPKAKTVANPVIVLFNKPFDVLCQFTDEQ
GRKTLKDYIPVPGVYAAGRLDRDSEGLLLLTNDGKLQSQITEPKKKTYKTYWAQVEGAPDDSDLEKLRQGVELKDGMTLP
AKISIMDNPAIWPRTPPIRERANIPTTWLEIQICEGRNRQVRRMTAHIGFPTLRLIRYKIGQWSLDDLQNGEYRQLDMK

Sequences:

>Translated_239_residues
MTQPFSKNRTTNKNNKTSSSGFARNGSNKPATVAKTPNKNDKPHNRAISKARPKAKTVANPVIVLFNKPFDVLCQFTDEQ
GRKTLKDYIPVPGVYAAGRLDRDSEGLLLLTNDGKLQSQITEPKKKTYKTYWAQVEGAPDDSDLEKLRQGVELKDGMTLP
AKISIMDNPAIWPRTPPIRERANIPTTWLEIQICEGRNRQVRRMTAHIGFPTLRLIRYKIGQWSLDDLQNGEYRQLDMK
>Mature_238_residues
TQPFSKNRTTNKNNKTSSSGFARNGSNKPATVAKTPNKNDKPHNRAISKARPKAKTVANPVIVLFNKPFDVLCQFTDEQG
RKTLKDYIPVPGVYAAGRLDRDSEGLLLLTNDGKLQSQITEPKKKTYKTYWAQVEGAPDDSDLEKLRQGVELKDGMTLPA
KISIMDNPAIWPRTPPIRERANIPTTWLEIQICEGRNRQVRRMTAHIGFPTLRLIRYKIGQWSLDDLQNGEYRQLDMK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=175, Percent_Identity=67.4285714285714, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1787524, Length=180, Percent_Identity=32.7777777777778, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788510, Length=183, Percent_Identity=34.4262295081967, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790453, Length=176, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 27119; Mature: 26988

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPFSKNRTTNKNNKTSSSGFARNGSNKPATVAKTPNKNDKPHNRAISKARPKAKTVAN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCHHHHCC
PVIVLFNKPFDVLCQFTDEQGRKTLKDYIPVPGVYAAGRLDRDSEGLLLLTNDGKLQSQI
CEEEEECCCHHHEEECCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCHHHHH
TEPKKKTYKTYWAQVEGAPDDSDLEKLRQGVELKDGMTLPAKISIMDNPAIWPRTPPIRE
CCHHHHHHHHHHEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHH
RANIPTTWLEIQICEGRNRQVRRMTAHIGFPTLRLIRYKIGQWSLDDLQNGEYRQLDMK
HCCCCEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHCCCCCEEEECCC
>Mature Secondary Structure 
TQPFSKNRTTNKNNKTSSSGFARNGSNKPATVAKTPNKNDKPHNRAISKARPKAKTVAN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCHHHHCC
PVIVLFNKPFDVLCQFTDEQGRKTLKDYIPVPGVYAAGRLDRDSEGLLLLTNDGKLQSQI
CEEEEECCCHHHEEECCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCHHHHH
TEPKKKTYKTYWAQVEGAPDDSDLEKLRQGVELKDGMTLPAKISIMDNPAIWPRTPPIRE
CCHHHHHHHHHHEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHH
RANIPTTWLEIQICEGRNRQVRRMTAHIGFPTLRLIRYKIGQWSLDDLQNGEYRQLDMK
HCCCCEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]