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The map label for this gene is fliA [H]

Identifier: 167623396

GI number: 167623396

Start: 1763302

End: 1764021

Strand: Direct

Name: fliA [H]

Synonym: Shal_1465

Alternate gene names: 167623396

Gene position: 1763302-1764021 (Clockwise)

Preceding gene: 167623395

Following gene: 167623397

Centisome position: 33.74

GC content: 46.53

Gene sequence:

>720_bases
GTGAATAAAGCGGCAGCGTATACTCGTTTTGATAATAAGACGTCTATCGTTGAACAGTATGCACCGCTTGTAAAAAGAAT
AGCCCATCATCTTTTGGCTCGTTTACCCGCATCAGTTCAGTTAGATGACTTGTTGCAGGCCGGCATGATGGGGCTACTCG
AAGCGTCATCAAACTTTGATGGTAGTAAGGGCGCCAAATTTGAAACCTTTGCAGGAATTCGAATTCGTGGCTCCATGTTA
GATGAAATCAGGCGTGGCGACTGGGTACCGCGTTCGGTACATCGTAATCAGCGCCGCGTGGCTCAAGTGATTGATGAGCT
TGAGCAAGAGCTTGGGCGTGATGCTCGTGATGGCGAGATTGCCGAGCGGTTAGAGATGCCGCTCGATGAGTATTACAGCA
TTTTGAACGACGTGTCGGTAGGTAAAGTCGTTGGAATAGAAGATTTAGGCGTTGCCGTTGAAGGATTAAATCAAGATGAC
GGCCACGAAGATGATGCTTATGAATCACTCGCAGAGGTCGAATTTCATTCGGCTCTTGTCGCAGCGATCAAACTATTACC
CGAAAGAGATGCGCTAGTCTTGTCTCTATACTATGATGAAGCATTGAATTTAAAAGAAATTGGGGCCATTCTTGAGGTCA
GTGAGTCTAGAGTTAGCCAGATCCATAGTCAGGCGATGCTCAGACTCAAGGGAAAACTCAAGCATTGGACGCAAGAATAA

Upstream 100 bases:

>100_bases
AAGGTTATGAGCTGGCCAGTCCCGTCACAACCCGGTGGCCATTTAGAGTTCTTCGTTGAGCGTTTGGTGCAGCGTCCAAG
CTATCAAGAGGATAAGTCGG

Downstream 100 bases:

>100_bases
AATCATAATAATTGATACCACATTACATGCGCATTTAACGCGCGCTCCGCGGAGGAAACCTTGGACAAGAATATGAAGAT
TCTTGTTGTTGACGATTTTT

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNKAAAYTRFDNKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSNFDGSKGAKFETFAGIRIRGSML
DEIRRGDWVPRSVHRNQRRVAQVIDELEQELGRDARDGEIAERLEMPLDEYYSILNDVSVGKVVGIEDLGVAVEGLNQDD
GHEDDAYESLAEVEFHSALVAAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQIHSQAMLRLKGKLKHWTQE

Sequences:

>Translated_239_residues
MNKAAAYTRFDNKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSNFDGSKGAKFETFAGIRIRGSML
DEIRRGDWVPRSVHRNQRRVAQVIDELEQELGRDARDGEIAERLEMPLDEYYSILNDVSVGKVVGIEDLGVAVEGLNQDD
GHEDDAYESLAEVEFHSALVAAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQIHSQAMLRLKGKLKHWTQE
>Mature_239_residues
MNKAAAYTRFDNKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSNFDGSKGAKFETFAGIRIRGSML
DEIRRGDWVPRSVHRNQRRVAQVIDELEQELGRDARDGEIAERLEMPLDEYYSILNDVSVGKVVGIEDLGVAVEGLNQDD
GHEDDAYESLAEVEFHSALVAAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQIHSQAMLRLKGKLKHWTQE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=226, Percent_Identity=50.8849557522124, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=26.4957264957265, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26755; Mature: 26755

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAAAYTRFDNKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSNFD
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
GSKGAKFETFAGIRIRGSMLDEIRRGDWVPRSVHRNQRRVAQVIDELEQELGRDARDGEI
CCCCCCEEHCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AERLEMPLDEYYSILNDVSVGKVVGIEDLGVAVEGLNQDDGHEDDAYESLAEVEFHSALV
HHHHCCCHHHHHHHHHCCCCCCEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
AAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQIHSQAMLRLKGKLKHWTQE
HHHHHCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKAAAYTRFDNKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSNFD
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
GSKGAKFETFAGIRIRGSMLDEIRRGDWVPRSVHRNQRRVAQVIDELEQELGRDARDGEI
CCCCCCEEHCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AERLEMPLDEYYSILNDVSVGKVVGIEDLGVAVEGLNQDDGHEDDAYESLAEVEFHSALV
HHHHCCCHHHHHHHHHCCCCCCEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
AAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQIHSQAMLRLKGKLKHWTQE
HHHHHCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]