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The map label for this gene is hydA [H]

Identifier: 167623339

GI number: 167623339

Start: 1701957

End: 1703189

Strand: Direct

Name: hydA [H]

Synonym: Shal_1408

Alternate gene names: 167623339

Gene position: 1701957-1703189 (Clockwise)

Preceding gene: 167623336

Following gene: 167623340

Centisome position: 32.56

GC content: 45.74

Gene sequence:

>1233_bases
ATGACTGTCACCACCTATCAACCCGGAGAGATCCAGGGGCTGATTGAGATACAGGCATCCAAATGTAAAGGTTGCGATGC
TTGTCAGAAATTTTGCCCTACCAATGCCATCGAAGGCGCATCAGGGGCTGTACACTCGATTAATAAAGAGAAGTGCTTGA
GTTGTGGCCAATGTCTCATCAATTGTCCTTTTGGAGCAATTATCGAGACCCACTCTGCACTAGAAACTGTAATTGCAAAA
CTGGCGGATAAAGACGTTAAAGTCGTGGGCATTATTGCCCCTGCTGTACGTGTTGCCATCGGTGAAGAGTTTGGCTTAGG
CACTGGTGAATTAGTGACTGGTAAGCTGTACGGCGCTATGAATAAAGCAGGTTTCAAGATTTTCGATTGTAACTTTGCCG
CTGACCTAACCATTATGGAAGAGGGAAGTGAGTTCATACACCGCTTGCACGCCAACGTGAAAGGGGAAGCTGATGCCGGT
GCATTACCTCAATTTACTTCTTGTTGTCCAGGTTGGGTTCGCTACCTAGAAACCAACTACCCAAGTTTGCTACCAAACTT
ATCAACAGCCAAGTCTCCACAGCAGATGGCTGGAACAGTAGCCAAAACTTATGGTGCAAAAGTTTATGCCATGCAACCAG
AAAACATTTTCACTGTTGCAGTTATGCCTTGTACCTCTAAAAAAGTAGAAGCGTCTCGTCCAGAGTTCAATTCAGCTTGG
AAATATAATCAAGAGCAGGGTGCCAATGTAGCAGAGTATCGAGATGTTGATGCTGTACTGACCACTCGTGAAATGGCGCA
GCTGTTAAAGTTACTAGAGATCGACCTAGCAAAAACACCTGAATACGAAGGCGATAGCTTATTCTCTGAATATACTGGCG
CAGGTACCATTTTTGGTGCGACAGGCGGTGTAATGGAGGCTGCGCTGCGTACAGCACACAAGGTTTTGACTGGCGATGAA
ATGGCCAAACTTGAGTTCGAACCTGTACGTGGTTTAGCGGGTGTAAAAGCTGCATCTGTGACGTTATTTGACCAAAGTTT
ATCGCAAGATGTCACTGTTAACGTGGCGGTTGTGCATGATATGGGGAATAACGTAGAGCCGATTTTACGAGACATAATGG
CTGGCATTTCTCCTTATCACTTCATTGAAGTGATGAATTGCGCTGGTGGTTGCGTAAACGGCGGTGGCCAGCCTATTGAC
GGCAATGGTTCATCTTGGATTGGTAATATCTAA

Upstream 100 bases:

>100_bases
TTCTGCCGACTTTTTATTACTAACTAAAAATAAAGCCTATTAGAACTAAGGTTTAAACTAACTAGTAACTAATGGCTCAT
AATAAGGAACGCGTAAATCT

Downstream 100 bases:

>100_bases
TAAGGAGCAACACCATGAACAACAAAAAGCATGTGTTTGCTGAAGATAGTTTTTTTATATCCCGTCGCAAATTTATGGCC
ATCGGTGCGGCTTTTATCGC

Product: hydrogenase, Fe-only

Products: NA

Alternate protein names: Fe hydrogenlyase [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTVTTYQPGEIQGLIEIQASKCKGCDACQKFCPTNAIEGASGAVHSINKEKCLSCGQCLINCPFGAIIETHSALETVIAK
LADKDVKVVGIIAPAVRVAIGEEFGLGTGELVTGKLYGAMNKAGFKIFDCNFAADLTIMEEGSEFIHRLHANVKGEADAG
ALPQFTSCCPGWVRYLETNYPSLLPNLSTAKSPQQMAGTVAKTYGAKVYAMQPENIFTVAVMPCTSKKVEASRPEFNSAW
KYNQEQGANVAEYRDVDAVLTTREMAQLLKLLEIDLAKTPEYEGDSLFSEYTGAGTIFGATGGVMEAALRTAHKVLTGDE
MAKLEFEPVRGLAGVKAASVTLFDQSLSQDVTVNVAVVHDMGNNVEPILRDIMAGISPYHFIEVMNCAGGCVNGGGQPID
GNGSSWIGNI

Sequences:

>Translated_410_residues
MTVTTYQPGEIQGLIEIQASKCKGCDACQKFCPTNAIEGASGAVHSINKEKCLSCGQCLINCPFGAIIETHSALETVIAK
LADKDVKVVGIIAPAVRVAIGEEFGLGTGELVTGKLYGAMNKAGFKIFDCNFAADLTIMEEGSEFIHRLHANVKGEADAG
ALPQFTSCCPGWVRYLETNYPSLLPNLSTAKSPQQMAGTVAKTYGAKVYAMQPENIFTVAVMPCTSKKVEASRPEFNSAW
KYNQEQGANVAEYRDVDAVLTTREMAQLLKLLEIDLAKTPEYEGDSLFSEYTGAGTIFGATGGVMEAALRTAHKVLTGDE
MAKLEFEPVRGLAGVKAASVTLFDQSLSQDVTVNVAVVHDMGNNVEPILRDIMAGISPYHFIEVMNCAGGCVNGGGQPID
GNGSSWIGNI
>Mature_409_residues
TVTTYQPGEIQGLIEIQASKCKGCDACQKFCPTNAIEGASGAVHSINKEKCLSCGQCLINCPFGAIIETHSALETVIAKL
ADKDVKVVGIIAPAVRVAIGEEFGLGTGELVTGKLYGAMNKAGFKIFDCNFAADLTIMEEGSEFIHRLHANVKGEADAGA
LPQFTSCCPGWVRYLETNYPSLLPNLSTAKSPQQMAGTVAKTYGAKVYAMQPENIFTVAVMPCTSKKVEASRPEFNSAWK
YNQEQGANVAEYRDVDAVLTTREMAQLLKLLEIDLAKTPEYEGDSLFSEYTGAGTIFGATGGVMEAALRTAHKVLTGDEM
AKLEFEPVRGLAGVKAASVTLFDQSLSQDVTVNVAVVHDMGNNVEPILRDIMAGISPYHFIEVMNCAGGCVNGGGQPIDG
NGSSWIGNI

Specific function: May be involved in hydrogen uptake for the reduction of sulfate to hydrogen sulfide in an electron transport chain. Cytochrome c3 is likely to be the physiological electron carrier for the enzyme [H]

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI11968051, Length=360, Percent_Identity=33.6111111111111, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI6912524, Length=390, Percent_Identity=28.4615384615385, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI134284357, Length=298, Percent_Identity=30.8724832214765, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI84452151, Length=298, Percent_Identity=30.8724832214765, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI14165461, Length=427, Percent_Identity=27.1662763466042, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71995015, Length=356, Percent_Identity=26.685393258427, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324089, Length=391, Percent_Identity=25.0639386189258, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI116007468, Length=370, Percent_Identity=27.2972972972973, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI116007470, Length=370, Percent_Identity=27.2972972972973, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI116007466, Length=370, Percent_Identity=27.2972972972973, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI116007464, Length=370, Percent_Identity=27.2972972972973, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI116007462, Length=240, Percent_Identity=32.0833333333333, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR013352 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF00037 Fer4 [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 43645; Mature: 43514

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTTYQPGEIQGLIEIQASKCKGCDACQKFCPTNAIEGASGAVHSINKEKCLSCGQCLI
CEEECCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHH
NCPFGAIIETHSALETVIAKLADKDVKVVGIIAPAVRVAIGEEFGLGTGELVTGKLYGAM
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHCCCCCCCCCCEEEHHHHHHH
NKAGFKIFDCNFAADLTIMEEGSEFIHRLHANVKGEADAGALPQFTSCCPGWVRYLETNY
CCCCCEEEECCCEECEEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHCCC
PSLLPNLSTAKSPQQMAGTVAKTYGAKVYAMQPENIFTVAVMPCTSKKVEASRPEFNSAW
HHHCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCC
KYNQEQGANVAEYRDVDAVLTTREMAQLLKLLEIDLAKTPEYEGDSLFSEYTGAGTIFGA
CCCHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEC
TGGVMEAALRTAHKVLTGDEMAKLEFEPVRGLAGVKAASVTLFDQSLSQDVTVNVAVVHD
CCHHHHHHHHHHHHHHCCCCHHEEECCCCCCCCCCCEEEEEEEHHHCCCCCEEEEEEEEC
MGNNVEPILRDIMAGISPYHFIEVMNCAGGCVNGGGQPIDGNGSSWIGNI
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVTTYQPGEIQGLIEIQASKCKGCDACQKFCPTNAIEGASGAVHSINKEKCLSCGQCLI
EEECCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHH
NCPFGAIIETHSALETVIAKLADKDVKVVGIIAPAVRVAIGEEFGLGTGELVTGKLYGAM
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHCCCCCCCCCCEEEHHHHHHH
NKAGFKIFDCNFAADLTIMEEGSEFIHRLHANVKGEADAGALPQFTSCCPGWVRYLETNY
CCCCCEEEECCCEECEEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHCCC
PSLLPNLSTAKSPQQMAGTVAKTYGAKVYAMQPENIFTVAVMPCTSKKVEASRPEFNSAW
HHHCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCC
KYNQEQGANVAEYRDVDAVLTTREMAQLLKLLEIDLAKTPEYEGDSLFSEYTGAGTIFGA
CCCHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEC
TGGVMEAALRTAHKVLTGDEMAKLEFEPVRGLAGVKAASVTLFDQSLSQDVTVNVAVVHD
CCHHHHHHHHHHHHHHCCCCHHEEECCCCCCCCCCCEEEEEEEHHHCCCCCEEEEEEEEC
MGNNVEPILRDIMAGISPYHFIEVMNCAGGCVNGGGQPIDGNGSSWIGNI
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3888621; 3322275; 11456963; 10368269; 11456758 [H]