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The map label for this gene is alaS [H]

Identifier: 167623167

GI number: 167623167

Start: 1499301

End: 1501925

Strand: Direct

Name: alaS [H]

Synonym: Shal_1233

Alternate gene names: 167623167

Gene position: 1499301-1501925 (Clockwise)

Preceding gene: 167623166

Following gene: 167623168

Centisome position: 28.68

GC content: 46.63

Gene sequence:

>2625_bases
ATGTATCAAACCACTGCAGCACTGCGAAGTGCTTTTTTGGAATATTTCCGCACAAATGGTCATCAGGTAGTTGATAGTAG
CTCTCTCGTTCCAGCGAACGATCCAACACTACTATTTACTAATGCGGGTATGAACCAGTTTAAAGATGTGTTTTTGGGTG
CAGATAAGCGTAGCTATTCTCGTGCAACGTCTTCACAGCGCTGTGTACGCGCGGGCGGTAAGCACAACGATTTAGATAAC
GTTGGCTACACTGCACGTCACCACACATTCTTCGAAATGTTAGGTAACTTCAGCTTTGGTGATTATTTTAAAGAAGAAGC
CATTCACTTTGCTTGGACTTTCCTTACTGAAGAATTAAAGCTACCGAAAGAGCGCCTTTGCGTCACCATCTATGAGACAG
ATGACGAAGCATTTGAAATTTGGAATAAGAAAATTGGCGTAGCTGCGGAAAATATTATTCGCATTGGCGATAACAAAGGC
GCAGCTTACGCATCAGATAACTTCTGGCAAATGGGTGACACAGGTCCTTGTGGTCCATGCTCTGAAATCTTCTACGATCA
CGGCGATCATATCTGGGGCGGACGTCCTGGCACACCGGAAGAAGATGGCGACCGTTTCATTGAGATCTGGAACATCGTAT
TCATGCAATACAACCGTCAAGCTGACGGTGAGATGAAACCACTGCCTAAGCCATCTGTTGATACGGGTATGGGGATTGAG
CGTATCGCTGCCATTATGCAGGGTGTACATTCAAATTATGAAATTGATATTTTCCAAGCCTTGATCAAGAAAACGGCTGC
TATTCTTGGCGTGACCGATCTTGAGAACAAATCACTTCGCGTCATTGCCGATCACATCCGTTCATGTGCTTTCTTAATTG
CAGATGGTGTCATGCCGTCGAATGAAGGCCGTGGTTATGTGCTTCGTCGTATTATCCGCCGCGCAGTGCGCCACGGTAAT
AAGCTAGGTGCTACTGAAAGCTTCTTCTATAAGTTGGTACCAACATTGATTGAAGTGATGGGCGATGCAGCTAAAGGCTT
ACAAGAGACTCAGGCTATCGTAGAGAAGTCACTTAAAGCTGAAGAAGAGCAGTTCGCGCGTACATTGGAGCGTGGCTTAG
GTATTTTGGATGCGGCACTCAATGAACTAACGACCAAGGTGTTAGACGGTGAGACAGCATTTAAACTATACGACACCTAC
GGTTTCCCTGTCGATTTGACCGCTGATGTTTGTCGTGAGCGTGAAATCACTGTAGATGAAGCGGGCTTTGAAGCCGCCAT
GGCCGAGCAACGTAAGCGTGCACAAGCTGCAGGTCAATTTGATACCGACTATAACGAAGGTCTTAAGATTGACGAGCAAA
GTAGCTTTACAGGCTACACCGAACTCAATAACCAAGCTACTGTAACGGCTATCTTCAAATCAGGTGAAGCTGCAGAGACT
ATTACTGCGGGTGACGAAGCGGTTATCGTTCTCGATAACACACCATTTTATGGTGAGTCGGGCGGCCAGAGTGGTGACAA
AGGTGTATTGATTGCAGATGGCATCGAGTTCACTGTTATTGATACACAAAAGTATGGCCAAGCAATTGGTCATATCGGCC
GTGTAGTCACTGGCGAAATTAAAGCGGGTCAGTCATTAACTGCCAACGTTGATAAGAAGCTACGTCATAGAACTGAGCTT
AACCACTCTGTGACTCACCTTTTACATGCGGCTCTTCGCCAAGTGCTTGGTGCTCACGTGAGTCAGAAAGGTTCTTTGGT
CGATCCTGAGCGTCTACGTTTTGACTTCTCTCATTTTGAAGGTGTTAAAGCCAATGAGCTTAAAGAAGTAGAAGAGCTCG
TGAATACTCAAATTCGTCGTAACCACGAATTGACCGCCGAAGTCATGGATATCGAAACCGCCAAAGAGAAAGGTGCAATG
GCACTGTTTGGTGAGAAGTACGATTCTGAAGTACGTGTTGTGACTATGGGTGACTTCTCTATCGAACTTTGCGGAGGTAC
TCACGTAGGTCGTACCGGTGATATCGGTTTGTTCAAGATCACTTCTGAAGGTGGTATCGCTGCCGGTATTCGTCGTATTG
AGGCGGTAACGGGTGCAGCGGCAATGGCATATGTTGCTAAGCAGCAAGCACAACTAGAAGAGGCGGCGACCCTATTAAAA
GGTGATAGCGCTTCAGTAGTCGCTAAGCTAAAAGCACAGCTTGATAAGACTAAGCAGCTTGAAAAAGAGCTGTCTCAACT
AAAAGACAAGCTTGCAGCGGCGACCAGTGCCGATCTTGCAGGTGAAGCTGTCGAGGTTAATGGCGTAAAGGTTTTAGTTA
AGAAGCTTGAAGGCGTCGATGCGGGCGCGCTGCGTGGCCTACAAGATGAGCTTAAGCAGAAGCTTCAATCTGGTATTGTG
GTTTTAGCTATCGCTGGTGAAGACAAGGTTAACTTGATTGTTGGCGTGACGAAGGATTTAACTGGTAAAGTTAAAGCTGG
CGAGTTAGTCGCTTCTATCGCTGTTCAAGTTGGTGGTAAAGGTGGTGGGCGTCCTGATATGGCTCAAGCAGGCGGTAGCC
AGCCAGAGAACTTAGACGGTGCGTTAGAGCAAGTTATCCCATGGATCACGGCTAAGCTGTCTTAA

Upstream 100 bases:

>100_bases
GGCTGGTTGTTAGCCATGACTCTGCTTATAATGCTATCCATAAAAATTTATCTCTCAGCTCTTTAAGGGCTATTTGCACA
ACCTAATTCAGGACGGTTTC

Downstream 100 bases:

>100_bases
ATAGTCGGATACCTTAAATTTAGAAACGCCCTTTATGGGCGTTTTTTTATGGAATAAGCAAACTGCTATAGACTCTTTTC
AACCACGCACACGTAGCTAA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 874; Mature: 874

Protein sequence:

>874_residues
MYQTTAALRSAFLEYFRTNGHQVVDSSSLVPANDPTLLFTNAGMNQFKDVFLGADKRSYSRATSSQRCVRAGGKHNDLDN
VGYTARHHTFFEMLGNFSFGDYFKEEAIHFAWTFLTEELKLPKERLCVTIYETDDEAFEIWNKKIGVAAENIIRIGDNKG
AAYASDNFWQMGDTGPCGPCSEIFYDHGDHIWGGRPGTPEEDGDRFIEIWNIVFMQYNRQADGEMKPLPKPSVDTGMGIE
RIAAIMQGVHSNYEIDIFQALIKKTAAILGVTDLENKSLRVIADHIRSCAFLIADGVMPSNEGRGYVLRRIIRRAVRHGN
KLGATESFFYKLVPTLIEVMGDAAKGLQETQAIVEKSLKAEEEQFARTLERGLGILDAALNELTTKVLDGETAFKLYDTY
GFPVDLTADVCREREITVDEAGFEAAMAEQRKRAQAAGQFDTDYNEGLKIDEQSSFTGYTELNNQATVTAIFKSGEAAET
ITAGDEAVIVLDNTPFYGESGGQSGDKGVLIADGIEFTVIDTQKYGQAIGHIGRVVTGEIKAGQSLTANVDKKLRHRTEL
NHSVTHLLHAALRQVLGAHVSQKGSLVDPERLRFDFSHFEGVKANELKEVEELVNTQIRRNHELTAEVMDIETAKEKGAM
ALFGEKYDSEVRVVTMGDFSIELCGGTHVGRTGDIGLFKITSEGGIAAGIRRIEAVTGAAAMAYVAKQQAQLEEAATLLK
GDSASVVAKLKAQLDKTKQLEKELSQLKDKLAAATSADLAGEAVEVNGVKVLVKKLEGVDAGALRGLQDELKQKLQSGIV
VLAIAGEDKVNLIVGVTKDLTGKVKAGELVASIAVQVGGKGGGRPDMAQAGGSQPENLDGALEQVIPWITAKLS

Sequences:

>Translated_874_residues
MYQTTAALRSAFLEYFRTNGHQVVDSSSLVPANDPTLLFTNAGMNQFKDVFLGADKRSYSRATSSQRCVRAGGKHNDLDN
VGYTARHHTFFEMLGNFSFGDYFKEEAIHFAWTFLTEELKLPKERLCVTIYETDDEAFEIWNKKIGVAAENIIRIGDNKG
AAYASDNFWQMGDTGPCGPCSEIFYDHGDHIWGGRPGTPEEDGDRFIEIWNIVFMQYNRQADGEMKPLPKPSVDTGMGIE
RIAAIMQGVHSNYEIDIFQALIKKTAAILGVTDLENKSLRVIADHIRSCAFLIADGVMPSNEGRGYVLRRIIRRAVRHGN
KLGATESFFYKLVPTLIEVMGDAAKGLQETQAIVEKSLKAEEEQFARTLERGLGILDAALNELTTKVLDGETAFKLYDTY
GFPVDLTADVCREREITVDEAGFEAAMAEQRKRAQAAGQFDTDYNEGLKIDEQSSFTGYTELNNQATVTAIFKSGEAAET
ITAGDEAVIVLDNTPFYGESGGQSGDKGVLIADGIEFTVIDTQKYGQAIGHIGRVVTGEIKAGQSLTANVDKKLRHRTEL
NHSVTHLLHAALRQVLGAHVSQKGSLVDPERLRFDFSHFEGVKANELKEVEELVNTQIRRNHELTAEVMDIETAKEKGAM
ALFGEKYDSEVRVVTMGDFSIELCGGTHVGRTGDIGLFKITSEGGIAAGIRRIEAVTGAAAMAYVAKQQAQLEEAATLLK
GDSASVVAKLKAQLDKTKQLEKELSQLKDKLAAATSADLAGEAVEVNGVKVLVKKLEGVDAGALRGLQDELKQKLQSGIV
VLAIAGEDKVNLIVGVTKDLTGKVKAGELVASIAVQVGGKGGGRPDMAQAGGSQPENLDGALEQVIPWITAKLS
>Mature_874_residues
MYQTTAALRSAFLEYFRTNGHQVVDSSSLVPANDPTLLFTNAGMNQFKDVFLGADKRSYSRATSSQRCVRAGGKHNDLDN
VGYTARHHTFFEMLGNFSFGDYFKEEAIHFAWTFLTEELKLPKERLCVTIYETDDEAFEIWNKKIGVAAENIIRIGDNKG
AAYASDNFWQMGDTGPCGPCSEIFYDHGDHIWGGRPGTPEEDGDRFIEIWNIVFMQYNRQADGEMKPLPKPSVDTGMGIE
RIAAIMQGVHSNYEIDIFQALIKKTAAILGVTDLENKSLRVIADHIRSCAFLIADGVMPSNEGRGYVLRRIIRRAVRHGN
KLGATESFFYKLVPTLIEVMGDAAKGLQETQAIVEKSLKAEEEQFARTLERGLGILDAALNELTTKVLDGETAFKLYDTY
GFPVDLTADVCREREITVDEAGFEAAMAEQRKRAQAAGQFDTDYNEGLKIDEQSSFTGYTELNNQATVTAIFKSGEAAET
ITAGDEAVIVLDNTPFYGESGGQSGDKGVLIADGIEFTVIDTQKYGQAIGHIGRVVTGEIKAGQSLTANVDKKLRHRTEL
NHSVTHLLHAALRQVLGAHVSQKGSLVDPERLRFDFSHFEGVKANELKEVEELVNTQIRRNHELTAEVMDIETAKEKGAM
ALFGEKYDSEVRVVTMGDFSIELCGGTHVGRTGDIGLFKITSEGGIAAGIRRIEAVTGAAAMAYVAKQQAQLEEAATLLK
GDSASVVAKLKAQLDKTKQLEKELSQLKDKLAAATSADLAGEAVEVNGVKVLVKKLEGVDAGALRGLQDELKQKLQSGIV
VLAIAGEDKVNLIVGVTKDLTGKVKAGELVASIAVQVGGKGGGRPDMAQAGGSQPENLDGALEQVIPWITAKLS

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=974, Percent_Identity=38.1930184804928, Blast_Score=556, Evalue=1e-158,
Organism=Homo sapiens, GI38569417, Length=765, Percent_Identity=36.9934640522876, Blast_Score=440, Evalue=1e-123,
Organism=Escherichia coli, GI1789048, Length=875, Percent_Identity=68.2285714285714, Blast_Score=1241, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=974, Percent_Identity=36.7556468172485, Blast_Score=528, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17536681, Length=744, Percent_Identity=34.9462365591398, Blast_Score=410, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324911, Length=852, Percent_Identity=40.4929577464789, Blast_Score=586, Evalue=1e-168,
Organism=Drosophila melanogaster, GI24582809, Length=978, Percent_Identity=38.0368098159509, Blast_Score=565, Evalue=1e-161,
Organism=Drosophila melanogaster, GI45552267, Length=978, Percent_Identity=38.0368098159509, Blast_Score=565, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24658214, Length=869, Percent_Identity=29.4591484464902, Blast_Score=322, Evalue=6e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 95107; Mature: 95107

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQTTAALRSAFLEYFRTNGHQVVDSSSLVPANDPTLLFTNAGMNQFKDVFLGADKRSYS
CCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHH
RATSSQRCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKEEAIHFAWTFLTEELK
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
LPKERLCVTIYETDDEAFEIWNKKIGVAAENIIRIGDNKGAAYASDNFWQMGDTGPCGPC
CCHHHEEEEEEECCCHHHHHHHHHCCCCHHCEEEECCCCCCEEECCCCEECCCCCCCCCH
SEIFYDHGDHIWGGRPGTPEEDGDRFIEIWNIVFMQYNRQADGEMKPLPKPSVDTGMGIE
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
RIAAIMQGVHSNYEIDIFQALIKKTAAILGVTDLENKSLRVIADHIRSCAFLIADGVMPS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
NEGRGYVLRRIIRRAVRHGNKLGATESFFYKLVPTLIEVMGDAAKGLQETQAIVEKSLKA
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEEQFARTLERGLGILDAALNELTTKVLDGETAFKLYDTYGFPVDLTADVCREREITVDE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCCEECC
AGFEAAMAEQRKRAQAAGQFDTDYNEGLKIDEQSSFTGYTELNNQATVTAIFKSGEAAET
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEEEECCCCCCE
ITAGDEAVIVLDNTPFYGESGGQSGDKGVLIADGIEFTVIDTQKYGQAIGHIGRVVTGEI
EECCCCEEEEECCCCCCCCCCCCCCCCCEEEECCEEEEEECCHHHHHHHHHHHHHEECCC
KAGQSLTANVDKKLRHRTELNHSVTHLLHAALRQVLGAHVSQKGSLVDPERLRFDFSHFE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEHHHCC
GVKANELKEVEELVNTQIRRNHELTAEVMDIETAKEKGAMALFGEKYDSEVRVVTMGDFS
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCEE
IELCGGTHVGRTGDIGLFKITSEGGIAAGIRRIEAVTGAAAMAYVAKQQAQLEEAATLLK
EEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDSASVVAKLKAQLDKTKQLEKELSQLKDKLAAATSADLAGEAVEVNGVKVLVKKLEGVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCC
AGALRGLQDELKQKLQSGIVVLAIAGEDKVNLIVGVTKDLTGKVKAGELVASIAVQVGGK
HHHHCCHHHHHHHHHHCCEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCC
GGGRPDMAQAGGSQPENLDGALEQVIPWITAKLS
CCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYQTTAALRSAFLEYFRTNGHQVVDSSSLVPANDPTLLFTNAGMNQFKDVFLGADKRSYS
CCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHH
RATSSQRCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKEEAIHFAWTFLTEELK
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
LPKERLCVTIYETDDEAFEIWNKKIGVAAENIIRIGDNKGAAYASDNFWQMGDTGPCGPC
CCHHHEEEEEEECCCHHHHHHHHHCCCCHHCEEEECCCCCCEEECCCCEECCCCCCCCCH
SEIFYDHGDHIWGGRPGTPEEDGDRFIEIWNIVFMQYNRQADGEMKPLPKPSVDTGMGIE
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
RIAAIMQGVHSNYEIDIFQALIKKTAAILGVTDLENKSLRVIADHIRSCAFLIADGVMPS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
NEGRGYVLRRIIRRAVRHGNKLGATESFFYKLVPTLIEVMGDAAKGLQETQAIVEKSLKA
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEEQFARTLERGLGILDAALNELTTKVLDGETAFKLYDTYGFPVDLTADVCREREITVDE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCCEECC
AGFEAAMAEQRKRAQAAGQFDTDYNEGLKIDEQSSFTGYTELNNQATVTAIFKSGEAAET
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEEEECCCCCCE
ITAGDEAVIVLDNTPFYGESGGQSGDKGVLIADGIEFTVIDTQKYGQAIGHIGRVVTGEI
EECCCCEEEEECCCCCCCCCCCCCCCCCEEEECCEEEEEECCHHHHHHHHHHHHHEECCC
KAGQSLTANVDKKLRHRTELNHSVTHLLHAALRQVLGAHVSQKGSLVDPERLRFDFSHFE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEHHHCC
GVKANELKEVEELVNTQIRRNHELTAEVMDIETAKEKGAMALFGEKYDSEVRVVTMGDFS
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCEE
IELCGGTHVGRTGDIGLFKITSEGGIAAGIRRIEAVTGAAAMAYVAKQQAQLEEAATLLK
EEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDSASVVAKLKAQLDKTKQLEKELSQLKDKLAAATSADLAGEAVEVNGVKVLVKKLEGVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCC
AGALRGLQDELKQKLQSGIVVLAIAGEDKVNLIVGVTKDLTGKVKAGELVASIAVQVGGK
HHHHCCHHHHHHHHHHCCEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCC
GGGRPDMAQAGGSQPENLDGALEQVIPWITAKLS
CCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA