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The map label for this gene is rpoS [H]

Identifier: 167623164

GI number: 167623164

Start: 1493719

End: 1494684

Strand: Direct

Name: rpoS [H]

Synonym: Shal_1230

Alternate gene names: 167623164

Gene position: 1493719-1494684 (Clockwise)

Preceding gene: 167623163

Following gene: 167623166

Centisome position: 28.58

GC content: 42.65

Gene sequence:

>966_bases
ATGGGCAGATTAAATAGTGCCGTTGTAGTGGAGCAAACCAAGAACCACTCTGATGAGTCAACGAATAAACTTACTAAACA
GGATGTGAAAGAGGCTGAATTAGAACAGCAAGTACAAGATGATCTACAAAAAAACTTAGATGCAACCCAATTGTATCTAG
GCGAAATTGGTTTTTCCCCCCTCCTCAGTGCTGAGGAAGAAGTATATTTTTCACGAAAGTCCTTAAAAGGCTGTGAAAAA
TCTCGCAATCGTATGATTGAAAGTAACCTCAGGTTGGTAGTAAAAATTGCTCGTCGATATAACAATCGAGGCTTAGCGCT
GCTCGATCTTATCGAAGAGGGTAATTTGGGCTTGATCCGTGCTGTAGAGAAGTTTGACCCCGAGCGTGGTTTTAGATTCT
CAACTTATGCAACATGGTGGATTAGGCAGACGATTGAACGAGCAATTATGAATCAGACTCGTACAATCCGATTACCCATC
CATGTAGTCAAAGAGCTTAATGTCTATTTGCGTACGGCGAGAGAATTAGCACATAAGCTAGATCACGAGCCAACGGCCGA
AGAGATCGCAGAACAACTCGATTTATCGAGTAGCGATGTTAGCCGTATGCTCAAGCTTAATGAAAGAATTACCTCTGTCG
ATACACCACTGGGTGGTGAAAGCGATAAAGCCTTATTAGATGTACTCGCAGATGATGATAACGTAGGACCCGATTATAAG
GTTCAGGATGAAGACATATCTAAGTCTGTGGTTAAGTGGTTAGATGAACTCAATACCAAACAACGAGAAGTGCTAGCGCG
CCGATTTGGTTTGTTAGGGTATGAGCCGTCGACTCTAGAGAATGTAGGAAAAGAGATTGGTTTGACTCGTGAGAGAGTAC
GCCAAATCCAAGTTGAGGCGCTTAAGCGTTTAAAAGATTTACTTGGGGCGCAGGGCCTTTCTGTAGAGGCAATTTTTAGA
GTGTAA

Upstream 100 bases:

>100_bases
GTTAAATTTTTGCCTAAACAATAGTTGTTGGGCTGCCAATTTGGCAAATCGGAGGATAGTAAAGTGCAGTAGTTAAATGT
AAAAATTCGGGAGAGATATT

Downstream 100 bases:

>100_bases
AAAGTAAATGTAAGATAATTAAAAAGCGCAGCTGATTATCAACTGCGCTTTTTTTGTGTCTAAAATATACTGAATTTTGA
TTTAGTGCACTTTGGCTTAA

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MGRLNSAVVVEQTKNHSDESTNKLTKQDVKEAELEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKSLKGCEK
SRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPI
HVVKELNVYLRTARELAHKLDHEPTAEEIAEQLDLSSSDVSRMLKLNERITSVDTPLGGESDKALLDVLADDDNVGPDYK
VQDEDISKSVVKWLDELNTKQREVLARRFGLLGYEPSTLENVGKEIGLTRERVRQIQVEALKRLKDLLGAQGLSVEAIFR
V

Sequences:

>Translated_321_residues
MGRLNSAVVVEQTKNHSDESTNKLTKQDVKEAELEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKSLKGCEK
SRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPI
HVVKELNVYLRTARELAHKLDHEPTAEEIAEQLDLSSSDVSRMLKLNERITSVDTPLGGESDKALLDVLADDDNVGPDYK
VQDEDISKSVVKWLDELNTKQREVLARRFGLLGYEPSTLENVGKEIGLTRERVRQIQVEALKRLKDLLGAQGLSVEAIFR
V
>Mature_320_residues
GRLNSAVVVEQTKNHSDESTNKLTKQDVKEAELEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKSLKGCEKS
RNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIH
VVKELNVYLRTARELAHKLDHEPTAEEIAEQLDLSSSDVSRMLKLNERITSVDTPLGGESDKALLDVLADDDNVGPDYKV
QDEDISKSVVKWLDELNTKQREVLARRFGLLGYEPSTLENVGKEIGLTRERVRQIQVEALKRLKDLLGAQGLSVEAIFRV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=289, Percent_Identity=79.2387543252595, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1789448, Length=226, Percent_Identity=42.0353982300885, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1789871, Length=286, Percent_Identity=27.6223776223776, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36660; Mature: 36529

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLNSAVVVEQTKNHSDESTNKLTKQDVKEAELEQQVQDDLQKNLDATQLYLGEIGFSP
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCH
LLSAEEEVYFSRKSLKGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIR
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCHHHH
AVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRTARELAHKL
HHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHC
DHEPTAEEIAEQLDLSSSDVSRMLKLNERITSVDTPLGGESDKALLDVLADDDNVGPDYK
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCE
VQDEDISKSVVKWLDELNTKQREVLARRFGLLGYEPSTLENVGKEIGLTRERVRQIQVEA
ECHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
LKRLKDLLGAQGLSVEAIFRV
HHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
GRLNSAVVVEQTKNHSDESTNKLTKQDVKEAELEQQVQDDLQKNLDATQLYLGEIGFSP
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCH
LLSAEEEVYFSRKSLKGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIR
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCHHHH
AVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRTARELAHKL
HHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHC
DHEPTAEEIAEQLDLSSSDVSRMLKLNERITSVDTPLGGESDKALLDVLADDDNVGPDYK
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCE
VQDEDISKSVVKWLDELNTKQREVLARRFGLLGYEPSTLENVGKEIGLTRERVRQIQVEA
ECHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
LKRLKDLLGAQGLSVEAIFRV
HHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]