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The map label for this gene is pilT [H]

Identifier: 167623108

GI number: 167623108

Start: 1426489

End: 1427601

Strand: Direct

Name: pilT [H]

Synonym: Shal_1174

Alternate gene names: 167623108

Gene position: 1426489-1427601 (Clockwise)

Preceding gene: 167623107

Following gene: 167623109

Centisome position: 27.29

GC content: 48.34

Gene sequence:

>1113_bases
ATGGATGTTCGTCCTTTTCTAAAGGTGATGATTGAGCGTAAGGCATCGGATCTGTTTATTACAGCAGGTTTTCCGCCAAG
CGCAAAAGTTGATGGTGAGCTGCGCCCACTCGGTGAGAATCCATTTACTCCTGCGCAGTCGTTAGACTTTGTTGAGTCGT
TAATGACTGAGGCACAGAAGCAAGAGTTTCATGGAACTCGCGAGTGTAACTTTGCCTTTTCCGCAAAAGATCTTGGTCGC
TTCCGTGTCAGTGCCTTTTGGCAGCGTGAGTTTCCCGGCTGCGTAATGCGTCGTATTGAGACACAGATCCCTGAGGTTGA
TGACTTAATGTTGCCGCCTATCTTAAAAGACTTAGTGATGAGTAAGCGCGGCCTAGTGATCTTGGTTGGTGGTACGGGAA
CGGGTAAATCGACTTCGCTTGCTGCACTGGTCGGTTATCGAAATTCCCACGCCCGTGGTCATATTCTTACCATCGAAGAT
CCGGTTGAATTTGTTCATGAACACAAGCAGAGCATTATTACTCAGCGTGAGGTGGGGATTGATACTGAATCTTTTGATGC
TGCGCTCAAAAGCTCACTGCGTCAGGCGCCGGATGTGATTTTGATTGGTGAAATTCGTAGTCAAGAAACCATGGAGTTTG
CACTGTCGTTTGCTGAAACCGGCCACCTTTGTATGGCGACCTTGCACGCTAACAACGCTAACCAAGCACTGGATCGCATC
ATGCATTTAGTACCTGAGAGCAAGCATCATCAACTGCTGTTTGATTTGTCGTTAAACCTGCGTGGTATTGTGGCGCAGCA
GCTGGTGCCCAAGAGCGATGGTAGCGGACGGCGTGCGGCGATAGAGGTGTTAATTAACACGCCGCGTGTGGCAAGCTTGA
TCGCCAAGAATGAGCTTCACTTGCTTAAAGACACCATGGCAAAGTCAAACGAGCAAGGGATGCAGACCTTCGACCAAGCG
TTATTAAAGCTCTATAGCGATGATGAGATTAGTTACGCCGATGCCTTGCATTATGCTGACTCACCAAACGATCTGCGCTT
GATGATTAAACTGCAAAGCTCCGATCCAAGCAGTAATAGCTCGATGGATGGAGTCACACTCGATTTAGACTAG

Upstream 100 bases:

>100_bases
GCGGCCAGATCACTCGTGAAGATGCCATGGCCAAGAGCTCGAATAAGCAAGCAAACTTTTAAGTAAGTAGTAAGTAATAA
GTAATAGGAATAGTGAGTCT

Downstream 100 bases:

>100_bases
TTTGAGTGGATTATTAGAAGACTGGCAATACCTTGTATTGCCAGTTTTTTTTGAGTCATAAAGAGCGTTGATTGTATCAA
CAGATTTTCTATGAGTTAAG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MDVRPFLKVMIERKASDLFITAGFPPSAKVDGELRPLGENPFTPAQSLDFVESLMTEAQKQEFHGTRECNFAFSAKDLGR
FRVSAFWQREFPGCVMRRIETQIPEVDDLMLPPILKDLVMSKRGLVILVGGTGTGKSTSLAALVGYRNSHARGHILTIED
PVEFVHEHKQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELHLLKDTMAKSNEQGMQTFDQA
LLKLYSDDEISYADALHYADSPNDLRLMIKLQSSDPSSNSSMDGVTLDLD

Sequences:

>Translated_370_residues
MDVRPFLKVMIERKASDLFITAGFPPSAKVDGELRPLGENPFTPAQSLDFVESLMTEAQKQEFHGTRECNFAFSAKDLGR
FRVSAFWQREFPGCVMRRIETQIPEVDDLMLPPILKDLVMSKRGLVILVGGTGTGKSTSLAALVGYRNSHARGHILTIED
PVEFVHEHKQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELHLLKDTMAKSNEQGMQTFDQA
LLKLYSDDEISYADALHYADSPNDLRLMIKLQSSDPSSNSSMDGVTLDLD
>Mature_370_residues
MDVRPFLKVMIERKASDLFITAGFPPSAKVDGELRPLGENPFTPAQSLDFVESLMTEAQKQEFHGTRECNFAFSAKDLGR
FRVSAFWQREFPGCVMRRIETQIPEVDDLMLPPILKDLVMSKRGLVILVGGTGTGKSTSLAALVGYRNSHARGHILTIED
PVEFVHEHKQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELHLLKDTMAKSNEQGMQTFDQA
LLKLYSDDEISYADALHYADSPNDLRLMIKLQSSDPSSNSSMDGVTLDLD

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=35.8255451713396, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1789723, Length=158, Percent_Identity=41.1392405063291, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1786296, Length=283, Percent_Identity=30.3886925795053, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41055; Mature: 41055

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRPFLKVMIERKASDLFITAGFPPSAKVDGELRPLGENPFTPAQSLDFVESLMTEAQK
CCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHH
QEFHGTRECNFAFSAKDLGRFRVSAFWQREFPGCVMRRIETQIPEVDDLMLPPILKDLVM
HHCCCCCCCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHH
SKRGLVILVGGTGTGKSTSLAALVGYRNSHARGHILTIEDPVEFVHEHKQSIITQREVGI
CCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCC
DTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELH
HHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
LLKDTMAKSNEQGMQTFDQALLKLYSDDEISYADALHYADSPNDLRLMIKLQSSDPSSNS
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCC
SMDGVTLDLD
CCCCEEEECC
>Mature Secondary Structure
MDVRPFLKVMIERKASDLFITAGFPPSAKVDGELRPLGENPFTPAQSLDFVESLMTEAQK
CCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHH
QEFHGTRECNFAFSAKDLGRFRVSAFWQREFPGCVMRRIETQIPEVDDLMLPPILKDLVM
HHCCCCCCCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHH
SKRGLVILVGGTGTGKSTSLAALVGYRNSHARGHILTIEDPVEFVHEHKQSIITQREVGI
CCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCC
DTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELH
HHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
LLKDTMAKSNEQGMQTFDQALLKLYSDDEISYADALHYADSPNDLRLMIKLQSSDPSSNS
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCC
SMDGVTLDLD
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]