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The map label for this gene is ispH

Identifier: 167623068

GI number: 167623068

Start: 1377834

End: 1378763

Strand: Direct

Name: ispH

Synonym: Shal_1134

Alternate gene names: 167623068

Gene position: 1377834-1378763 (Clockwise)

Preceding gene: 167623067

Following gene: 167623069

Centisome position: 26.36

GC content: 48.71

Gene sequence:

>930_bases
ATGAATATTCTTTTGGCAAACCCACGTGGTTTCTGTGCGGGCGTCGATCGTGCTATTAGCATTGTTGAGCGAGCATTAGA
ACTATTTTCGCCCCCTATTTACGTACGACATGAAGTGGTGCATAACCGCTATGTGGTGCAGAATCTAAAAGATCGCGGTG
CAGTATTTGTTGAAGAACTGGATCAGGTGCCTGACGAGAGTATCGTCATCTTTAGTGCTCACGGCGTATCTCAAGCGGTT
CGCCACGAGGCTAAAAGACGTGGCCTTAAAGTGTTTGATGCAACCTGTCCGCTAGTCACCAAGGTTCACCTACAGGTTGC
AAGGGCTAGCCGTAAAGGCATTGAGTGCATCTTAATTGGCCACGCCGGCCATCCTGAAGTTGAAGGTACCATGGGGCAGT
ATGATAACCCCGCTGGCGGCGTTCATTTAGTTGAGTCGCCAGAAGACGTTGCTAATCTTGTGGTTAAAGATCCCGATAAT
CTGTGTTTTGTTACTCAAACCACCTTGTCTATGGATGATACGACTGATGTGATTGCATCCTTGCAAAGCAAGTACCCTTC
AATCGAGGGGCCGCGTAAGGACGATATCTGTTATGCAACGCAGAACCGCCAAGATGCGGTACGTAATGTGGCAGAAAAAG
TTGAGTTGTTTATCGTGGTGGGCTCAAAGAACAGTTCAAATTCGAACCGTCTGCGTGAGTTAGCACAGAAGAAAGGTACA
ACGGCTTTCTTAGTCGATAACGCCGACGATGTTGATCCGTCATGGTTTAACGGTATTCAACATGTCGCCGTCACAGCTGG
GGCTTCGGCACCTGAGGTACTGGTTAAGCAGGTTGTTGATGCTATAGCTAAAATGGCACCGAGTGTGATCACCGAGGTCG
AAGGACGTCTAGAGGACACTGTGTTTGCTGTACCCGCAGAGCTACGCTGA

Upstream 100 bases:

>100_bases
TCAGAGATGTGGTTGGCGACTCGGTAACTATCGATCTGAACCACCCATTGGCGGGGCAAGTGCTCACCTTTGAACTCGAA
GTGGTACAGGTATTGTAATT

Downstream 100 bases:

>100_bases
TCTATAACGAAAGTTGGCTCCAAACTGAAATAAATAAAAAGAGGCTTAGGCCTCTTTTTTTTGCTTAAAAATTAGTGATT
TCAACGGTTGATAATACGAA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAVFVEELDQVPDESIVIFSAHGVSQAV
RHEAKRRGLKVFDATCPLVTKVHLQVARASRKGIECILIGHAGHPEVEGTMGQYDNPAGGVHLVESPEDVANLVVKDPDN
LCFVTQTTLSMDDTTDVIASLQSKYPSIEGPRKDDICYATQNRQDAVRNVAEKVELFIVVGSKNSSNSNRLRELAQKKGT
TAFLVDNADDVDPSWFNGIQHVAVTAGASAPEVLVKQVVDAIAKMAPSVITEVEGRLEDTVFAVPAELR

Sequences:

>Translated_309_residues
MNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAVFVEELDQVPDESIVIFSAHGVSQAV
RHEAKRRGLKVFDATCPLVTKVHLQVARASRKGIECILIGHAGHPEVEGTMGQYDNPAGGVHLVESPEDVANLVVKDPDN
LCFVTQTTLSMDDTTDVIASLQSKYPSIEGPRKDDICYATQNRQDAVRNVAEKVELFIVVGSKNSSNSNRLRELAQKKGT
TAFLVDNADDVDPSWFNGIQHVAVTAGASAPEVLVKQVVDAIAKMAPSVITEVEGRLEDTVFAVPAELR
>Mature_309_residues
MNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAVFVEELDQVPDESIVIFSAHGVSQAV
RHEAKRRGLKVFDATCPLVTKVHLQVARASRKGIECILIGHAGHPEVEGTMGQYDNPAGGVHLVESPEDVANLVVKDPDN
LCFVTQTTLSMDDTTDVIASLQSKYPSIEGPRKDDICYATQNRQDAVRNVAEKVELFIVVGSKNSSNSNRLRELAQKKGT
TAFLVDNADDVDPSWFNGIQHVAVTAGASAPEVLVKQVVDAIAKMAPSVITEVEGRLEDTVFAVPAELR

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=66.3430420711974, Blast_Score=432, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_SHEHH (B0TJB2)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673362.1
- GeneID:   5906563
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1134
- HOGENOM:   HBG335228
- OMA:   AAIHFKR
- ProtClustDB:   PRK01045
- BioCyc:   SHAL458817:SHAL_1134-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 33699; Mature: 33699

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAVFVEEL
CCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH
DQVPDESIVIFSAHGVSQAVRHEAKRRGLKVFDATCPLVTKVHLQVARASRKGIECILIG
HCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEE
HAGHPEVEGTMGQYDNPAGGVHLVESPEDVANLVVKDPDNLCFVTQTTLSMDDTTDVIAS
CCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHEEEECCCCEEEEEEEECCCCHHHHHHHH
LQSKYPSIEGPRKDDICYATQNRQDAVRNVAEKVELFIVVGSKNSSNSNRLRELAQKKGT
HHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHCCC
TAFLVDNADDVDPSWFNGIQHVAVTAGASAPEVLVKQVVDAIAKMAPSVITEVEGRLEDT
EEEEECCCCCCCCHHHHCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VFAVPAELR
EEECCCCCC
>Mature Secondary Structure
MNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAVFVEEL
CCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH
DQVPDESIVIFSAHGVSQAVRHEAKRRGLKVFDATCPLVTKVHLQVARASRKGIECILIG
HCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEE
HAGHPEVEGTMGQYDNPAGGVHLVESPEDVANLVVKDPDNLCFVTQTTLSMDDTTDVIAS
CCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHEEEECCCCEEEEEEEECCCCHHHHHHHH
LQSKYPSIEGPRKDDICYATQNRQDAVRNVAEKVELFIVVGSKNSSNSNRLRELAQKKGT
HHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHCCC
TAFLVDNADDVDPSWFNGIQHVAVTAGASAPEVLVKQVVDAIAKMAPSVITEVEGRLEDT
EEEEECCCCCCCCHHHHCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VFAVPAELR
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA