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The map label for this gene is nfo [H]

Identifier: 167623052

GI number: 167623052

Start: 1357977

End: 1358831

Strand: Reverse

Name: nfo [H]

Synonym: Shal_1118

Alternate gene names: 167623052

Gene position: 1358831-1357977 (Counterclockwise)

Preceding gene: 167623054

Following gene: 167623047

Centisome position: 26.0

GC content: 45.03

Gene sequence:

>855_bases
ATGCAAGCTTCACACCTTATTGGCGCCCATGTTAGCGCCGCTGGCGGTATCGCCAATGCTCCACTAAATGCCGCAGCGAT
TGGCGCGAATGCTTTTGCTCTGTTTACCAAAAACCAACGTCGCTGGCAGGCGAATGACCTCGAAGAGAAGCAAATATTAC
TATTCAAGCAGCATTGTCACGACGCTAATATCAGCCTCGAATCCATTTTGCCTCACGATAGTTACTTGATTAATTTGGGT
CACCCAGATGATGAGCTGTTAGAAAAATCTCGTCAGGCCTTCTATGACGAAATGCAACGCTGCGAACAGATAGGCCTTAG
TTTATTGAACTTTCACCCTGGTAGTCACCTAAGAAAAATACCTATCGATGACTGCTTAGCAAAAATTAGTGAATCGATAA
ACTTAGCCTTAGAACGTTCATCTGGCGTGACCGCTGTAATCGAAAATACCGCAGGCCAAGGTTCCAATCTTGGGCACTCA
TTCGAACAGTTAGCGCAGATCATCAATGGTGTGGAAGATAAGTCTAGGGTTGGCGTCTGTATCGATACTTGTCACCTGTT
TGCAGCAGGTTATGATATTCGCACCCAAGAGGCTTGCAAGCAAACCTTTGATCTTTTCGATAAGGTCGTGGGTAATCAAT
ACCTGAAAGCCATGCATTTGAATGACAGTAAAACAGCGCTTAATAGTAGAGTCGACCGCCATGAATCCTTAGGGCAAGGT
GAAATAGGTCAGCAAGCGTTTGCTAGCTTGATGAAGCTAGATGTGATTAAAGGGATCCCACTTATTTTAGAAACGATACG
CCCAGAGATCTGGCAGGAAGAGATTAACTGGTTACGTTTTCTCGCTAAGCAGTGA

Upstream 100 bases:

>100_bases
CGAGCACTCTGAAAATAAATATTCACAGAGTGATCTAAACCACTTTTACGCTACCGTGGCATAATACCAAAAATAGAAAT
CAATAACGGAACAGATCCAG

Downstream 100 bases:

>100_bases
TCTTAATTCCTAATTTGCTACATTAAACATTTGTAGATCACTGCAACCACTTTGGTAGGCTCGCCTAAGTCTGATTGCAA
CCATACGATATGGGTGCCTT

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MQASHLIGAHVSAAGGIANAPLNAAAIGANAFALFTKNQRRWQANDLEEKQILLFKQHCHDANISLESILPHDSYLINLG
HPDDELLEKSRQAFYDEMQRCEQIGLSLLNFHPGSHLRKIPIDDCLAKISESINLALERSSGVTAVIENTAGQGSNLGHS
FEQLAQIINGVEDKSRVGVCIDTCHLFAAGYDIRTQEACKQTFDLFDKVVGNQYLKAMHLNDSKTALNSRVDRHESLGQG
EIGQQAFASLMKLDVIKGIPLILETIRPEIWQEEINWLRFLAKQ

Sequences:

>Translated_284_residues
MQASHLIGAHVSAAGGIANAPLNAAAIGANAFALFTKNQRRWQANDLEEKQILLFKQHCHDANISLESILPHDSYLINLG
HPDDELLEKSRQAFYDEMQRCEQIGLSLLNFHPGSHLRKIPIDDCLAKISESINLALERSSGVTAVIENTAGQGSNLGHS
FEQLAQIINGVEDKSRVGVCIDTCHLFAAGYDIRTQEACKQTFDLFDKVVGNQYLKAMHLNDSKTALNSRVDRHESLGQG
EIGQQAFASLMKLDVIKGIPLILETIRPEIWQEEINWLRFLAKQ
>Mature_284_residues
MQASHLIGAHVSAAGGIANAPLNAAAIGANAFALFTKNQRRWQANDLEEKQILLFKQHCHDANISLESILPHDSYLINLG
HPDDELLEKSRQAFYDEMQRCEQIGLSLLNFHPGSHLRKIPIDDCLAKISESINLALERSSGVTAVIENTAGQGSNLGHS
FEQLAQIINGVEDKSRVGVCIDTCHLFAAGYDIRTQEACKQTFDLFDKVVGNQYLKAMHLNDSKTALNSRVDRHESLGQG
EIGQQAFASLMKLDVIKGIPLILETIRPEIWQEEINWLRFLAKQ

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=273, Percent_Identity=67.032967032967, Blast_Score=380, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17531193, Length=267, Percent_Identity=48.314606741573, Blast_Score=272, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6322735, Length=264, Percent_Identity=44.3181818181818, Blast_Score=234, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 31520; Mature: 31520

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASHLIGAHVSAAGGIANAPLNAAAIGANAFALFTKNQRRWQANDLEEKQILLFKQHCH
CCCCHHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCC
DANISLESILPHDSYLINLGHPDDELLEKSRQAFYDEMQRCEQIGLSLLNFHPGSHLRKI
CCCCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCC
PIDDCLAKISESINLALERSSGVTAVIENTAGQGSNLGHSFEQLAQIINGVEDKSRVGVC
CHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHCCHH
IDTCHLFAAGYDIRTQEACKQTFDLFDKVVGNQYLKAMHLNDSKTALNSRVDRHESLGQG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
EIGQQAFASLMKLDVIKGIPLILETIRPEIWQEEINWLRFLAKQ
CCCHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQASHLIGAHVSAAGGIANAPLNAAAIGANAFALFTKNQRRWQANDLEEKQILLFKQHCH
CCCCHHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCC
DANISLESILPHDSYLINLGHPDDELLEKSRQAFYDEMQRCEQIGLSLLNFHPGSHLRKI
CCCCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCC
PIDDCLAKISESINLALERSSGVTAVIENTAGQGSNLGHSFEQLAQIINGVEDKSRVGVC
CHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHCCHH
IDTCHLFAAGYDIRTQEACKQTFDLFDKVVGNQYLKAMHLNDSKTALNSRVDRHESLGQG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
EIGQQAFASLMKLDVIKGIPLILETIRPEIWQEEINWLRFLAKQ
CCCHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA