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The map label for this gene is nadB [H]

Identifier: 167623010

GI number: 167623010

Start: 1314482

End: 1316092

Strand: Reverse

Name: nadB [H]

Synonym: Shal_1076

Alternate gene names: 167623010

Gene position: 1316092-1314482 (Counterclockwise)

Preceding gene: 167623022

Following gene: 167623000

Centisome position: 25.18

GC content: 47.8

Gene sequence:

>1611_bases
ATGAAACAAGTAGTTGAACACCAATCTGACGTTTTGGTTATCGGCAGTGGCGCAGCCGGTCTCACTTTAGCTTTGCATCT
TGCTGAAAAATCAAAAGTTATTTTGCTATCCAAAGGGCCTCTCAGTGAAGGCTCAACCTATTACGCCCAAGGCGGTATCG
CCTCTGTATTTGACGAAGGTGACACAATTGAGTCGCACGTTGCCGACACTTTAGTTGCCGGAGCCGGCCTTTGTGACGAA
CCCGTCGTCAAATTTACCGCCGAAAACGCCAAAGCAGCAATGCAATGGTTAATTAATTGTGGCGTGGCCTTCGATAAAGA
AGAAGTTCCCGCTGGCAGTAATGTAGAAGCACCTTATCACCTAACCCGTGAAGGCGGCCATAGTCACCGACGCATTCTAC
ATGCCGCTGATGCGACAGGCAAAGAAGTCCAAGTGACTTTACAAGACCAAGCACGCAACCACCCTAATATTCGAGTACTC
GAACGCTATAACGCCATCGATCTCATTACCACTCGTAAATTAAGCCTACCGGGAAACCGAGTGCTAGGTGCCTATATATG
GAACCGAGATGAAGAGCATGTTGAAACGGTTAAGGCGCGTTTCGTTGCCCTAGCAACAGGCGGCAGCTCTAAGGTTTATC
AATATACCTCTAATCCAGATATCGCCTCTGGTGACGGTATTGCTATGGCCTGGCGCTCTGGTTGCCGCGTTGCCAATATG
GAATTTAACCAATTCCATCCGACATGCCTATACCATGCCGATGCACGCAACTTTTTGCTTACCGAAGCCCTTCGCGGCGA
AGGGGCATTTTTACGTCGCCCAGATAGCTCTCGCTTTATGCCTGACTTTGATGAGCGCGCAGAACTTGCCCCCCGTGACA
TTGTCGCGCGAGCAATCGACTATGAGATGAAGCGTTTAGGCTCTGACTGTGTCTACCTAGACATTACCCATAAGCCAGCA
GACTTTGTTATTAAGCATTTTCCAACAATCTATAAGCGCTGTTTAGAACTGGGCATCGATATCACCAAAGAGCCTATCCC
AGTGGTCCCCGCGGCGCATTATACCTGCGGTGGGGTGATGACAGATCTACATGGCCAAACTGATCTTAATGGTTTGTACG
CCATTGGCGAGGTCGCATATACAGGCCTTCATGGTGCCAACCGTTTAGCCAGTAACTCACTGCTTGAATGTTTGGTATTT
GCTAGAGCTGCGGCTGAAGATATTGAAAGTCAGCTAGGTAAAATCCCAATGCCAGGGACTCTACCTGCTTGGGATGAAAG
CCAAGTATCTAATTCAGATGAAGAAGTGGTTATCGCTCATAACTGGCATGAGTTACGCCTGTTTATGTGGGATTACGTCG
GAATTGTTCGTTCTGATAAACGGCTTGAGCGCGCGCTACGTCGCTGCGTGATGTTACAGCAGGAAATTCAAGAGTATTAC
AGCAACTTTAGAGTCAGTAATAACTTGCTAGAGCTACGTAACTTAGTCCAAGTTGCAGAGCTTATTATTCGCTGCGCGAT
GGAACGTAAAGAAAGCCGAGGCTTACATTACAACATTGATCATCCAAACAAGGATGCATCACCAAAGCCTACTATTCTAC
AGCCCGATTAG

Upstream 100 bases:

>100_bases
TTATAGAAGCGTTGATTTCTTTATATACACCTATGAAAGTATCCATATTCAGCGCCATGGTTTAACATAGTCGGACTCCC
AGATAACTCTATGATACCTG

Downstream 100 bases:

>100_bases
CCCTAGTCGCTAACCATAGTCATTAGCCTTTCACCTCTGGCTGGCTAACTGAAGTAGACGACACAAATGCCGGAAGTTTG
TGTCGTCTAGCATATCAGCC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MKQVVEHQSDVLVIGSGAAGLTLALHLAEKSKVILLSKGPLSEGSTYYAQGGIASVFDEGDTIESHVADTLVAGAGLCDE
PVVKFTAENAKAAMQWLINCGVAFDKEEVPAGSNVEAPYHLTREGGHSHRRILHAADATGKEVQVTLQDQARNHPNIRVL
ERYNAIDLITTRKLSLPGNRVLGAYIWNRDEEHVETVKARFVALATGGSSKVYQYTSNPDIASGDGIAMAWRSGCRVANM
EFNQFHPTCLYHADARNFLLTEALRGEGAFLRRPDSSRFMPDFDERAELAPRDIVARAIDYEMKRLGSDCVYLDITHKPA
DFVIKHFPTIYKRCLELGIDITKEPIPVVPAAHYTCGGVMTDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAAAEDIESQLGKIPMPGTLPAWDESQVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCVMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHYNIDHPNKDASPKPTILQPD

Sequences:

>Translated_536_residues
MKQVVEHQSDVLVIGSGAAGLTLALHLAEKSKVILLSKGPLSEGSTYYAQGGIASVFDEGDTIESHVADTLVAGAGLCDE
PVVKFTAENAKAAMQWLINCGVAFDKEEVPAGSNVEAPYHLTREGGHSHRRILHAADATGKEVQVTLQDQARNHPNIRVL
ERYNAIDLITTRKLSLPGNRVLGAYIWNRDEEHVETVKARFVALATGGSSKVYQYTSNPDIASGDGIAMAWRSGCRVANM
EFNQFHPTCLYHADARNFLLTEALRGEGAFLRRPDSSRFMPDFDERAELAPRDIVARAIDYEMKRLGSDCVYLDITHKPA
DFVIKHFPTIYKRCLELGIDITKEPIPVVPAAHYTCGGVMTDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAAAEDIESQLGKIPMPGTLPAWDESQVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCVMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHYNIDHPNKDASPKPTILQPD
>Mature_536_residues
MKQVVEHQSDVLVIGSGAAGLTLALHLAEKSKVILLSKGPLSEGSTYYAQGGIASVFDEGDTIESHVADTLVAGAGLCDE
PVVKFTAENAKAAMQWLINCGVAFDKEEVPAGSNVEAPYHLTREGGHSHRRILHAADATGKEVQVTLQDQARNHPNIRVL
ERYNAIDLITTRKLSLPGNRVLGAYIWNRDEEHVETVKARFVALATGGSSKVYQYTSNPDIASGDGIAMAWRSGCRVANM
EFNQFHPTCLYHADARNFLLTEALRGEGAFLRRPDSSRFMPDFDERAELAPRDIVARAIDYEMKRLGSDCVYLDITHKPA
DFVIKHFPTIYKRCLELGIDITKEPIPVVPAAHYTCGGVMTDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAAAEDIESQLGKIPMPGTLPAWDESQVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCVMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHYNIDHPNKDASPKPTILQPD

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=515, Percent_Identity=31.8446601941748, Blast_Score=191, Evalue=2e-48,
Organism=Escherichia coli, GI1788928, Length=536, Percent_Identity=71.2686567164179, Blast_Score=809, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=528, Percent_Identity=31.6287878787879, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1786942, Length=560, Percent_Identity=31.0714285714286, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17550100, Length=563, Percent_Identity=31.0834813499112, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17505833, Length=555, Percent_Identity=30.0900900900901, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI71986328, Length=428, Percent_Identity=28.2710280373832, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322701, Length=560, Percent_Identity=30.8928571428571, Blast_Score=211, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6322416, Length=565, Percent_Identity=30.2654867256637, Blast_Score=196, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6320788, Length=488, Percent_Identity=26.6393442622951, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137288, Length=562, Percent_Identity=30.9608540925267, Blast_Score=198, Evalue=7e-51,
Organism=Drosophila melanogaster, GI24655642, Length=562, Percent_Identity=30.9608540925267, Blast_Score=198, Evalue=7e-51,
Organism=Drosophila melanogaster, GI24655647, Length=562, Percent_Identity=30.9608540925267, Blast_Score=198, Evalue=7e-51,
Organism=Drosophila melanogaster, GI24663005, Length=563, Percent_Identity=30.550621669627, Blast_Score=198, Evalue=9e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59544; Mature: 59544

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVVEHQSDVLVIGSGAAGLTLALHLAEKSKVILLSKGPLSEGSTYYAQGGIASVFDEG
CCCHHCCCCCEEEEECCCCCEEEEEEEECCCCEEEEECCCCCCCCEEEECCCCHHHHCCC
DTIESHVADTLVAGAGLCDEPVVKFTAENAKAAMQWLINCGVAFDKEEVPAGSNVEAPYH
CCHHHHHHHHHHHCCCCCCCCHHEEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEE
LTREGGHSHRRILHAADATGKEVQVTLQDQARNHPNIRVLERYNAIDLITTRKLSLPGNR
EECCCCCCHHHEEEECCCCCCEEEEEEHHHCCCCCCEEEEEECCCEEEEEEEEECCCCCC
VLGAYIWNRDEEHVETVKARFVALATGGSSKVYQYTSNPDIASGDGIAMAWRSGCRVANM
EEEEEEECCCHHHHHHHHHHEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEC
EFNQFHPTCLYHADARNFLLTEALRGEGAFLRRPDSSRFMPDFDERAELAPRDIVARAID
CCCCCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHCCHHHHHHHHHH
YEMKRLGSDCVYLDITHKPADFVIKHFPTIYKRCLELGIDITKEPIPVVPAAHYTCGGVM
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
TDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFARAAAEDIESQLGKIPMPGT
CCCCCCCCCCHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LPAWDESQVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCVMLQQEIQEYY
CCCCCCHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHYNIDHPNKDASPKPTILQPD
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQVVEHQSDVLVIGSGAAGLTLALHLAEKSKVILLSKGPLSEGSTYYAQGGIASVFDEG
CCCHHCCCCCEEEEECCCCCEEEEEEEECCCCEEEEECCCCCCCCEEEECCCCHHHHCCC
DTIESHVADTLVAGAGLCDEPVVKFTAENAKAAMQWLINCGVAFDKEEVPAGSNVEAPYH
CCHHHHHHHHHHHCCCCCCCCHHEEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEE
LTREGGHSHRRILHAADATGKEVQVTLQDQARNHPNIRVLERYNAIDLITTRKLSLPGNR
EECCCCCCHHHEEEECCCCCCEEEEEEHHHCCCCCCEEEEEECCCEEEEEEEEECCCCCC
VLGAYIWNRDEEHVETVKARFVALATGGSSKVYQYTSNPDIASGDGIAMAWRSGCRVANM
EEEEEEECCCHHHHHHHHHHEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEC
EFNQFHPTCLYHADARNFLLTEALRGEGAFLRRPDSSRFMPDFDERAELAPRDIVARAID
CCCCCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHCCHHHHHHHHHH
YEMKRLGSDCVYLDITHKPADFVIKHFPTIYKRCLELGIDITKEPIPVVPAAHYTCGGVM
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
TDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFARAAAEDIESQLGKIPMPGT
CCCCCCCCCCHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LPAWDESQVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCVMLQQEIQEYY
CCCCCCHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHYNIDHPNKDASPKPTILQPD
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]