Click here to switch to the map view.

The map label for this gene is hemL

Identifier: 167622960

GI number: 167622960

Start: 1252662

End: 1253951

Strand: Reverse

Name: hemL

Synonym: Shal_1021

Alternate gene names: 167622960

Gene position: 1253951-1252662 (Counterclockwise)

Preceding gene: 167622961

Following gene: 167622959

Centisome position: 23.99

GC content: 48.22

Gene sequence:

>1290_bases
ATGACCCGTTCCGACGAACTATTTGAACAAGCTAAAAAAACCATTCCTGGCGGCGTAAACTCTCCAGTCCGTGCATTTTC
AGGTGTGGGTGGCTCTCCACGCTTTATCGAAAAAGCTGATGGCGCTTATATCTTTGACGCCGATGGTAAAAAATATATCG
ACTATGTAGGTTCTTGGGGCCCAATGATCTTAGGCCACAACCACCCAAAAATTCGCGAAGCGGTATTAGCTGCTGTTGAA
AACGGCCTATCTTTCGGTGCACCAACTGAACTTGAAATCACTATGGCTGAAAAAGTCATCGAGATGGTGCCTTCAATAGA
GCAAGTTCGTATGGTGAGTTCAGGGACTGAAGCCACCATGAGTGCGATTCGTTTAGCACGCGGTTTTACTAACCGTGACA
AAATCCTTAAGTTTGAAGGTTGCTACCACGGCCACGCAGACTGCTTGTTAGTTAAAGCAGGTTCTGGCGCACTAACATTG
GGTCAGCCAAGCTCTCCAGGTATTCCTGAAGACTTTGCAAAGCACACCCTAACTGCTACTTACAACGATCTAGATTCAGT
TAAGGCTATCTTCGAGCAATACCCAGAAGAGATCTCTTGTATCATCATCGAGCCAGTTGCTGGCAACATGAACTGTATCC
CGCCAATCGACGGTTTCTTACAGGGTCTACGCGCTATCTGTGATCAGTACGGCGCACTGATGATTATCGACGAAGTGATG
ACGGGGTTCCGCGTATCTAAGAGCGGTGCACAAGGTCACTACGGCGTCACGCCAGATCTTACGACGCTAGGTAAAGTTAT
CGGCGGCGGCATGCCTGTGGGTGCTTTCGGTGGTCGTAAAGATGTGATGCAGTTTATCGCACCAACAGGCCCAGTTTACC
AAGCGGGTACGCTGTCTGGTAACCCAATTGCGATGTCGGCAGGCCTTGCACAGATGGAAGCGCTTTGTGAAGAAGGCGTT
TACGAGCAACTAGCGGCTAAGACTCAGTATATTGCAGAAGGTTTTAAAGCTGCGGCAAACAAGCATGGCATTCCAATGGC
AATCAACTACGTTGGTGGCATGTTTGGTTTCTTCTTCACAGACGAAGCGACTGTAACCAACTTTGCGCAAGTCACTAAGT
GTGACACTGCACTGTTTGCTGAGTTCTACCACCTAATGCTAGATGAGGGTGTTTACCTAGCACCTAGCGCTTATGAAGCA
GGTTTCCTATCACTTGCTCACGGTGAAGAAGAGCTTGAAGCGACACTAGCAGCTGCTGATCGCGTTTTTGCTAAGTTAGC
AAAACGCTAA

Upstream 100 bases:

>100_bases
GTTAGGTGTTGACTCTCAACTCGAGCAATATGAGCGTCCAGTAAATTGGTATTCACAAAAAGCTGAAATTGAATCAGGTA
CTGAATTTTAAGGATTAAAA

Downstream 100 bases:

>100_bases
TCGACTCGAAGTCTTAGCGCTGCTTAATCGCTAATAAAAGGAACACTTAGGTGTTCCTTTTTTGTATCCACCAGTTAGAT
TCCGACTCTTACCAGCACAC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTRSDELFEQAKKTIPGGVNSPVRAFSGVGGSPRFIEKADGAYIFDADGKKYIDYVGSWGPMILGHNHPKIREAVLAAVE
NGLSFGAPTELEITMAEKVIEMVPSIEQVRMVSSGTEATMSAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTL
GQPSSPGIPEDFAKHTLTATYNDLDSVKAIFEQYPEEISCIIIEPVAGNMNCIPPIDGFLQGLRAICDQYGALMIIDEVM
TGFRVSKSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKDVMQFIAPTGPVYQAGTLSGNPIAMSAGLAQMEALCEEGV
YEQLAAKTQYIAEGFKAAANKHGIPMAINYVGGMFGFFFTDEATVTNFAQVTKCDTALFAEFYHLMLDEGVYLAPSAYEA
GFLSLAHGEEELEATLAAADRVFAKLAKR

Sequences:

>Translated_429_residues
MTRSDELFEQAKKTIPGGVNSPVRAFSGVGGSPRFIEKADGAYIFDADGKKYIDYVGSWGPMILGHNHPKIREAVLAAVE
NGLSFGAPTELEITMAEKVIEMVPSIEQVRMVSSGTEATMSAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTL
GQPSSPGIPEDFAKHTLTATYNDLDSVKAIFEQYPEEISCIIIEPVAGNMNCIPPIDGFLQGLRAICDQYGALMIIDEVM
TGFRVSKSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKDVMQFIAPTGPVYQAGTLSGNPIAMSAGLAQMEALCEEGV
YEQLAAKTQYIAEGFKAAANKHGIPMAINYVGGMFGFFFTDEATVTNFAQVTKCDTALFAEFYHLMLDEGVYLAPSAYEA
GFLSLAHGEEELEATLAAADRVFAKLAKR
>Mature_428_residues
TRSDELFEQAKKTIPGGVNSPVRAFSGVGGSPRFIEKADGAYIFDADGKKYIDYVGSWGPMILGHNHPKIREAVLAAVEN
GLSFGAPTELEITMAEKVIEMVPSIEQVRMVSSGTEATMSAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTLG
QPSSPGIPEDFAKHTLTATYNDLDSVKAIFEQYPEEISCIIIEPVAGNMNCIPPIDGFLQGLRAICDQYGALMIIDEVMT
GFRVSKSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKDVMQFIAPTGPVYQAGTLSGNPIAMSAGLAQMEALCEEGVY
EQLAAKTQYIAEGFKAAANKHGIPMAINYVGGMFGFFFTDEATVTNFAQVTKCDTALFAEFYHLMLDEGVYLAPSAYEAG
FLSLAHGEEELEATLAAADRVFAKLAKR

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=302, Percent_Identity=28.476821192053, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI284507298, Length=222, Percent_Identity=28.3783783783784, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=70.18779342723, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=417, Percent_Identity=30.2158273381295, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1788044, Length=331, Percent_Identity=29.9093655589124, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789759, Length=332, Percent_Identity=30.421686746988, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787560, Length=351, Percent_Identity=30.7692307692308, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI145693181, Length=342, Percent_Identity=28.3625730994152, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1786991, Length=359, Percent_Identity=27.8551532033426, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=386, Percent_Identity=27.979274611399, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI25144271, Length=302, Percent_Identity=27.8145695364238, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32564660, Length=349, Percent_Identity=26.9340974212034, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=161, Percent_Identity=32.2981366459627, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=342, Percent_Identity=28.9473684210526, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=341, Percent_Identity=25.5131964809384, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21357415, Length=298, Percent_Identity=29.1946308724832, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21356575, Length=325, Percent_Identity=27.6923076923077, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_SHEHH (B0TIQ0)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673254.1
- ProteinModelPortal:   B0TIQ0
- SMR:   B0TIQ0
- GeneID:   5905947
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1021
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- BioCyc:   SHAL458817:SHAL_1021-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45837; Mature: 45706

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSDELFEQAKKTIPGGVNSPVRAFSGVGGSPRFIEKADGAYIFDADGKKYIDYVGSWG
CCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHCCCCCCEEECCCCCHHHHHHCCCC
PMILGHNHPKIREAVLAAVENGLSFGAPTELEITMAEKVIEMVPSIEQVRMVSSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTLGQPSSPGIPEDFAKHTLTAT
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHH
YNDLDSVKAIFEQYPEEISCIIIEPVAGNMNCIPPIDGFLQGLRAICDQYGALMIIDEVM
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHH
TGFRVSKSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKDVMQFIAPTGPVYQAGTLSG
HCCEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMSAGLAQMEALCEEGVYEQLAAKTQYIAEGFKAAANKHGIPMAINYVGGMFGFFFT
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCC
DEATVTNFAQVTKCDTALFAEFYHLMLDEGVYLAPSAYEAGFLSLAHGEEELEATLAAAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEHHCCHHHHHHHHHHHH
RVFAKLAKR
HHHHHHHCC
>Mature Secondary Structure 
TRSDELFEQAKKTIPGGVNSPVRAFSGVGGSPRFIEKADGAYIFDADGKKYIDYVGSWG
CCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHCCCCCCEEECCCCCHHHHHHCCCC
PMILGHNHPKIREAVLAAVENGLSFGAPTELEITMAEKVIEMVPSIEQVRMVSSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTLGQPSSPGIPEDFAKHTLTAT
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHH
YNDLDSVKAIFEQYPEEISCIIIEPVAGNMNCIPPIDGFLQGLRAICDQYGALMIIDEVM
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHH
TGFRVSKSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKDVMQFIAPTGPVYQAGTLSG
HCCEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMSAGLAQMEALCEEGVYEQLAAKTQYIAEGFKAAANKHGIPMAINYVGGMFGFFFT
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCC
DEATVTNFAQVTKCDTALFAEFYHLMLDEGVYLAPSAYEAGFLSLAHGEEELEATLAAAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEHHCCHHHHHHHHHHHH
RVFAKLAKR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA