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The map label for this gene is cca [H]

Identifier: 167622956

GI number: 167622956

Start: 1246573

End: 1247850

Strand: Reverse

Name: cca [H]

Synonym: Shal_1017

Alternate gene names: 167622956

Gene position: 1247850-1246573 (Counterclockwise)

Preceding gene: 167622959

Following gene: 167622955

Centisome position: 23.87

GC content: 47.89

Gene sequence:

>1278_bases
ATGCTACACTCGCCACTAAGTTATCAAGTGAGAATCGATGTGAAAATTTACCTTGTTGGCGGCGCAGTCCGCGATAAACT
ACTCAACCTTCCAGTAAAAGATCTTGACTATATGGTCGTCGGTGCGACACCTAAGCAGATGCTTGCACTTGGCTATAATC
AAGTAGGTAAAGATTTCCCCGTTTTTTTACATCCCAAGACTCAGCAAGAATATGCTCTGGCACGTACCGAGCGCAAAACT
GCAGCGGGCTACGGTGGATTTAGTGTCGATGCAGCGCCTAGCGTCACCTTAGAGCAAGATCTTATGCGCCGAGACTTAAC
GATCAACGCCATCGCACAAGATGAAGAAGGTAACCTCTACGATCCCTACCAAGGCATTCAAGCCATCGAAAAACGCAGCC
TAAGACACGTATCCGACGCCTTTGTCGAAGACCCATTAAGGGTATTGCGAGTCGCCCGCTTTGCCGCCCGTTTTCACTCC
CTTGGCTTTACCGTGGCACCAGAAACTCTGACGTTAATGGCTGAAATAAGCCAAAGCGGAGAACTGCAAGCCTTAACCGC
TGAGCGTGTCTATATTGAGTTAAACAAAGCATTAGCCACCGATAACCCACAAATATTCTTCCAAGTATTAAGAGACTGCG
GCGCACTGGCGGTGTTATTTCCGGAAATTGAAGCACTATTTGGTGTACCGCAACCTGAAAAATGGCATCCTGAAATCGAT
ACAGGTGTTCACACTATGATGGTGCTCGAACAAGTTGCCAAGCTAACCGATGATAACTCGGTGCGCTTTGCCGCATTGGT
ACACGATCTAGGTAAGGCATTAAGCCCTAAAGAGCACCTACCTAAACACCATGGTCACGGCCAAAAAGGACTCCCCTTAA
TCAAATCTCTGTGTGAGCGCTTTAGGGTACCTAATGAGTATCGTGACTTAGCGCTACTGGTCAGCGACCAACATCAAAAC
ATCCACAAGATCACCGAGCTTAGGGCCGATACCCTAGTGAAATTGTTTGATAAGGCCGATTTTTGGCGCAAACCACAACG
TCTTGAGCAGCTGCTAATCGCCTGTGAAGCTGACAGTAAAGGGCGCGCAGGACTTGAATTTAGCCCTTATCCTCAGGCTA
GTTACCTTAAGCAATGTTTTGCCGCCGCATCGGCAGTAGAGGTCAAGTCCATCATAGAGCAGGGCTATAAAGGTACAGAG
ATCCGCGAGCAGTTGAGCATCAAACGAATTGCTGCAGTACAGGAAGTTAAAGGCTTGCAGGCAATACCTGCAGAATAA

Upstream 100 bases:

>100_bases
AAAGTACGTTCAATGTCAAATTCAGCTCAGTATCTACCAATTTCTAAAGACATATTAGACATCCCCCCCTCAAGAATTAG
CCCTAGAGCCCAATGGATCC

Downstream 100 bases:

>100_bases
ATAAACATAAAAAGTTTACATTTATAGACTAGTATTAGAGGTGTATTATTTGTGAATAATTTACACTTCACTAGCAATTG
CTTAAATTTTCACTAAGTTA

Product: polynucleotide adenylyltransferase/metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MLHSPLSYQVRIDVKIYLVGGAVRDKLLNLPVKDLDYMVVGATPKQMLALGYNQVGKDFPVFLHPKTQQEYALARTERKT
AAGYGGFSVDAAPSVTLEQDLMRRDLTINAIAQDEEGNLYDPYQGIQAIEKRSLRHVSDAFVEDPLRVLRVARFAARFHS
LGFTVAPETLTLMAEISQSGELQALTAERVYIELNKALATDNPQIFFQVLRDCGALAVLFPEIEALFGVPQPEKWHPEID
TGVHTMMVLEQVAKLTDDNSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKSLCERFRVPNEYRDLALLVSDQHQN
IHKITELRADTLVKLFDKADFWRKPQRLEQLLIACEADSKGRAGLEFSPYPQASYLKQCFAAASAVEVKSIIEQGYKGTE
IREQLSIKRIAAVQEVKGLQAIPAE

Sequences:

>Translated_425_residues
MLHSPLSYQVRIDVKIYLVGGAVRDKLLNLPVKDLDYMVVGATPKQMLALGYNQVGKDFPVFLHPKTQQEYALARTERKT
AAGYGGFSVDAAPSVTLEQDLMRRDLTINAIAQDEEGNLYDPYQGIQAIEKRSLRHVSDAFVEDPLRVLRVARFAARFHS
LGFTVAPETLTLMAEISQSGELQALTAERVYIELNKALATDNPQIFFQVLRDCGALAVLFPEIEALFGVPQPEKWHPEID
TGVHTMMVLEQVAKLTDDNSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKSLCERFRVPNEYRDLALLVSDQHQN
IHKITELRADTLVKLFDKADFWRKPQRLEQLLIACEADSKGRAGLEFSPYPQASYLKQCFAAASAVEVKSIIEQGYKGTE
IREQLSIKRIAAVQEVKGLQAIPAE
>Mature_425_residues
MLHSPLSYQVRIDVKIYLVGGAVRDKLLNLPVKDLDYMVVGATPKQMLALGYNQVGKDFPVFLHPKTQQEYALARTERKT
AAGYGGFSVDAAPSVTLEQDLMRRDLTINAIAQDEEGNLYDPYQGIQAIEKRSLRHVSDAFVEDPLRVLRVARFAARFHS
LGFTVAPETLTLMAEISQSGELQALTAERVYIELNKALATDNPQIFFQVLRDCGALAVLFPEIEALFGVPQPEKWHPEID
TGVHTMMVLEQVAKLTDDNSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKSLCERFRVPNEYRDLALLVSDQHQN
IHKITELRADTLVKLFDKADFWRKPQRLEQLLIACEADSKGRAGLEFSPYPQASYLKQCFAAASAVEVKSIIEQGYKGTE
IREQLSIKRIAAVQEVKGLQAIPAE

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=213, Percent_Identity=26.7605633802817, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789436, Length=404, Percent_Identity=61.8811881188119, Blast_Score=528, Evalue=1e-151,
Organism=Drosophila melanogaster, GI281360112, Length=214, Percent_Identity=27.1028037383178, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357337, Length=214, Percent_Identity=27.1028037383178, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47529; Mature: 47529

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHSPLSYQVRIDVKIYLVGGAVRDKLLNLPVKDLDYMVVGATPKQMLALGYNQVGKDFP
CCCCCCCEEEEEEEEEEEECCHHHHHHHCCCHHHCCEEEECCCHHHHHHHHHHHCCCCCC
VFLHPKTQQEYALARTERKTAAGYGGFSVDAAPSVTLEQDLMRRDLTINAIAQDEEGNLY
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCC
DPYQGIQAIEKRSLRHVSDAFVEDPLRVLRVARFAARFHSLGFTVAPETLTLMAEISQSG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHCCCC
ELQALTAERVYIELNKALATDNPQIFFQVLRDCGALAVLFPEIEALFGVPQPEKWHPEID
CEEEEECCEEEEEEEHHHCCCCHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCHH
TGVHTMMVLEQVAKLTDDNSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKSLCER
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHH
FRVPNEYRDLALLVSDQHQNIHKITELRADTLVKLFDKADFWRKPQRLEQLLIACEADSK
HCCCCHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
GRAGLEFSPYPQASYLKQCFAAASAVEVKSIIEQGYKGTEIREQLSIKRIAAVQEVKGLQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
AIPAE
CCCCC
>Mature Secondary Structure
MLHSPLSYQVRIDVKIYLVGGAVRDKLLNLPVKDLDYMVVGATPKQMLALGYNQVGKDFP
CCCCCCCEEEEEEEEEEEECCHHHHHHHCCCHHHCCEEEECCCHHHHHHHHHHHCCCCCC
VFLHPKTQQEYALARTERKTAAGYGGFSVDAAPSVTLEQDLMRRDLTINAIAQDEEGNLY
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCC
DPYQGIQAIEKRSLRHVSDAFVEDPLRVLRVARFAARFHSLGFTVAPETLTLMAEISQSG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHCCCC
ELQALTAERVYIELNKALATDNPQIFFQVLRDCGALAVLFPEIEALFGVPQPEKWHPEID
CEEEEECCEEEEEEEHHHCCCCHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCHH
TGVHTMMVLEQVAKLTDDNSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKSLCER
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHH
FRVPNEYRDLALLVSDQHQNIHKITELRADTLVKLFDKADFWRKPQRLEQLLIACEADSK
HCCCCHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
GRAGLEFSPYPQASYLKQCFAAASAVEVKSIIEQGYKGTEIREQLSIKRIAAVQEVKGLQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
AIPAE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA