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The map label for this gene is rhtB [H]

Identifier: 167622809

GI number: 167622809

Start: 1085427

End: 1086041

Strand: Direct

Name: rhtB [H]

Synonym: Shal_0869

Alternate gene names: 167622809

Gene position: 1085427-1086041 (Clockwise)

Preceding gene: 167622806

Following gene: 167622811

Centisome position: 20.77

GC content: 47.32

Gene sequence:

>615_bases
ATGGCACTAGACACTTGGTTGATTTATCTCGTGGCAATTATTGGTTTGTCGCTCGCTCCCGGCCCTAATGGACTTATGGC
ATTAACTCATGGGGTAATGTATGGACATAGAAAGACGCTATTTACCATTACTGGAGGCGTGCTAGGTTTTGTTGTATTGA
TCGCGCTCACCATGTTTGGCATAGGCGCATTGCTGCTGGCCTCAGCGGATCTACTTTTAGTCTTTAAATGGATAGGTGGG
ATATACCTTATTTGGTTGGGGATACGTATTTGGCGCTCTCCGGCAATAGAGTTGCAACCCGTGACTAAGGCTCAATCAGT
GAGTGATCTGGCGCTATTTAGACAAGGAGCATTGGCAGCAATAACTAACCCTAAGGTGCTATTGTTTTTCGGTGCCTTCT
TGCCTCAATTTATCGAGCCCGAACAGCCACTGCTTGCGCAGTTTATCGTGATGGCCGCCACGTTCGCGGTGATAGAATTT
GTTGTCGAATACCTGATAGCAAGGGTCGCGACTAAGCTTCGTCCATGGTTAGCCCGTAGCGGCAAGCGTTTCAACCAGTG
TTGTGGCGGACTCTTTACTCTGATTGGAGCTAGCCTACCTCTATACAATAATTAA

Upstream 100 bases:

>100_bases
TATTACCTTATTTTGTCTTGATTGTATCAAGTGGCGATTCTTACCTTCCCCTATAATCCGTTTTTTATATAGATACTATC
GCTCAATTAAAGGTAAAAGT

Downstream 100 bases:

>100_bases
GAGGGGGATTTTGTGAGGGGAATTTTGGCGAGAAGATCTTGGCGAGAAGAGCTTGGTGAGGGGCCTTAAGCTGAATTAGG
ATTTGGTCGAGTTAGTCGAG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MALDTWLIYLVAIIGLSLAPGPNGLMALTHGVMYGHRKTLFTITGGVLGFVVLIALTMFGIGALLLASADLLLVFKWIGG
IYLIWLGIRIWRSPAIELQPVTKAQSVSDLALFRQGALAAITNPKVLLFFGAFLPQFIEPEQPLLAQFIVMAATFAVIEF
VVEYLIARVATKLRPWLARSGKRFNQCCGGLFTLIGASLPLYNN

Sequences:

>Translated_204_residues
MALDTWLIYLVAIIGLSLAPGPNGLMALTHGVMYGHRKTLFTITGGVLGFVVLIALTMFGIGALLLASADLLLVFKWIGG
IYLIWLGIRIWRSPAIELQPVTKAQSVSDLALFRQGALAAITNPKVLLFFGAFLPQFIEPEQPLLAQFIVMAATFAVIEF
VVEYLIARVATKLRPWLARSGKRFNQCCGGLFTLIGASLPLYNN
>Mature_203_residues
ALDTWLIYLVAIIGLSLAPGPNGLMALTHGVMYGHRKTLFTITGGVLGFVVLIALTMFGIGALLLASADLLLVFKWIGGI
YLIWLGIRIWRSPAIELQPVTKAQSVSDLALFRQGALAAITNPKVLLFFGAFLPQFIEPEQPLLAQFIVMAATFAVIEFV
VEYLIARVATKLRPWLARSGKRFNQCCGGLFTLIGASLPLYNN

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=200, Percent_Identity=37, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788099, Length=159, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22236; Mature: 22105

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDTWLIYLVAIIGLSLAPGPNGLMALTHGVMYGHRKTLFTITGGVLGFVVLIALTMFG
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHEEHHHHHHHHHHHHHHHHHH
IGALLLASADLLLVFKWIGGIYLIWLGIRIWRSPAIELQPVTKAQSVSDLALFRQGALAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCHHE
ITNPKVLLFFGAFLPQFIEPEQPLLAQFIVMAATFAVIEFVVEYLIARVATKLRPWLARS
ECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKRFNQCCGGLFTLIGASLPLYNN
CCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ALDTWLIYLVAIIGLSLAPGPNGLMALTHGVMYGHRKTLFTITGGVLGFVVLIALTMFG
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHEEHHHHHHHHHHHHHHHHHH
IGALLLASADLLLVFKWIGGIYLIWLGIRIWRSPAIELQPVTKAQSVSDLALFRQGALAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCHHE
ITNPKVLLFFGAFLPQFIEPEQPLLAQFIVMAATFAVIEFVVEYLIARVATKLRPWLARS
ECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKRFNQCCGGLFTLIGASLPLYNN
CCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]