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The map label for this gene is pgk

Identifier: 167622786

GI number: 167622786

Start: 1052725

End: 1053900

Strand: Direct

Name: pgk

Synonym: Shal_0846

Alternate gene names: 167622786

Gene position: 1052725-1053900 (Clockwise)

Preceding gene: 167622785

Following gene: 167622787

Centisome position: 20.14

GC content: 48.89

Gene sequence:

>1176_bases
ATGGCGATTCTTAATATGCAAGATCTTGAGCTTCAAGGTAAGCGCGTGCTTATTCGTGAAGATCTAAACGTACCAGTTAA
AGATGGCGTGGTTACCAGCGACGCGCGTTTACGCGCATCACTGCCTACCATCAAGCTGGCACTTGAAAAAGGCGCAGCGG
TTATGGTGATGTCTCACCTAGGTCGCCCAACAGAAGGCGAGTTCAATGCTGAATTTTCAATGCAGCCAGTGGTTGATTAC
CTAACAAAAGCACTTGATTGCCCTGTGAGCTTAGCAACGGAATACCTAGACGGCGTTGAAGTTGCCGTTGGCGAAGTGGT
TGTGTTTGAAAACGTGCGCTTTAACGTGGGCGAAAAGAAGAACGACGAAGCACTTGCTAAGAAGATGGCTGCACTGTGTG
ACGTATACGTGATGGATGCATTCGGTACTGCGCACCGCGCTCAAGCGTCAACTCACGGCGTGGGTCTACACGCGCCAATC
GCATGTGCTGGCCCATTGTTAGCAGGTGAGCTTGCTGCACTTGGTAAAGCACTTGATAACCCAGCGCGCCCAATGGTGGC
AATTGTCGGTGGTTCAAAAGTATCAACTAAGCTAACGGTACTTGAAAGCCTTTCAACTAAAGTTGACCAACTTGTTGTTG
GTGGTGGTATTGCTAACACTTTCGTTGCTGCTGCTGGTCACCAAGTCGGTAAGTCACTTTATGAAGCTGACCTGATTGAA
GAAGCTAAGCGCCTTGTTGCTAACGCGCAAAGCCGTGGTGGTGATATTCCAGTACCAACTGATGTGGTAGTCGCAAGCGA
GTTCAGCCCTACAGCGACTGCGACGCTAAAAGATGTTAGCACAGTATCTGACACTGATATGATTTTTGATATCGGTCCTG
ATAGCGCTGAAGCCCTTGCTGAGATCATCAAAAATGCCGGTACGGTTGTATGGAACGGCCCAGTTGGTGTATTTGAATTT
GATCAGTTTGGTGAAGGCACTAAGCGTATTGCTCAGGCGATTGCCGAGTCAAACGCCTTCTCTATCGCGGGTGGTGGTGA
CACATTAGCTGCGGTTGATAAGTATGGTATCGCCGATAAAGTGTCTTATATCTCAACAGGTGGCGGCGCTTTCCTTGAGT
TTTTAGAGGGCAAAGAGCTTCCTGCTGTAGCAATGCTCGAGAGTCGTGGACAGTAA

Upstream 100 bases:

>100_bases
CTGCTGTCTGTTAGCCCGATTGATTGAATTTTTAGCTCGACTTAAATTTTAACTTTAACTTTAACTTTAATTTTAACTTA
GATAGTAAGGAAGCATGAAA

Downstream 100 bases:

>100_bases
TCGATGGGATCTGCTGTTAATTGATACGAATCGATGAACAGTTACCAAAAAATTACCAAACTGTATGTTGGCGGCCTTTA
AATCGTACTATGCTACGGTT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAILNMQDLELQGKRVLIREDLNVPVKDGVVTSDARLRASLPTIKLALEKGAAVMVMSHLGRPTEGEFNAEFSMQPVVDY
LTKALDCPVSLATEYLDGVEVAVGEVVVFENVRFNVGEKKNDEALAKKMAALCDVYVMDAFGTAHRAQASTHGVGLHAPI
ACAGPLLAGELAALGKALDNPARPMVAIVGGSKVSTKLTVLESLSTKVDQLVVGGGIANTFVAAAGHQVGKSLYEADLIE
EAKRLVANAQSRGGDIPVPTDVVVASEFSPTATATLKDVSTVSDTDMIFDIGPDSAEALAEIIKNAGTVVWNGPVGVFEF
DQFGEGTKRIAQAIAESNAFSIAGGGDTLAAVDKYGIADKVSYISTGGGAFLEFLEGKELPAVAMLESRGQ

Sequences:

>Translated_391_residues
MAILNMQDLELQGKRVLIREDLNVPVKDGVVTSDARLRASLPTIKLALEKGAAVMVMSHLGRPTEGEFNAEFSMQPVVDY
LTKALDCPVSLATEYLDGVEVAVGEVVVFENVRFNVGEKKNDEALAKKMAALCDVYVMDAFGTAHRAQASTHGVGLHAPI
ACAGPLLAGELAALGKALDNPARPMVAIVGGSKVSTKLTVLESLSTKVDQLVVGGGIANTFVAAAGHQVGKSLYEADLIE
EAKRLVANAQSRGGDIPVPTDVVVASEFSPTATATLKDVSTVSDTDMIFDIGPDSAEALAEIIKNAGTVVWNGPVGVFEF
DQFGEGTKRIAQAIAESNAFSIAGGGDTLAAVDKYGIADKVSYISTGGGAFLEFLEGKELPAVAMLESRGQ
>Mature_390_residues
AILNMQDLELQGKRVLIREDLNVPVKDGVVTSDARLRASLPTIKLALEKGAAVMVMSHLGRPTEGEFNAEFSMQPVVDYL
TKALDCPVSLATEYLDGVEVAVGEVVVFENVRFNVGEKKNDEALAKKMAALCDVYVMDAFGTAHRAQASTHGVGLHAPIA
CAGPLLAGELAALGKALDNPARPMVAIVGGSKVSTKLTVLESLSTKVDQLVVGGGIANTFVAAAGHQVGKSLYEADLIEE
AKRLVANAQSRGGDIPVPTDVVVASEFSPTATATLKDVSTVSDTDMIFDIGPDSAEALAEIIKNAGTVVWNGPVGVFEFD
QFGEGTKRIAQAIAESNAFSIAGGGDTLAAVDKYGIADKVSYISTGGGAFLEFLEGKELPAVAMLESRGQ

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=39.4673123486683, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI4505763, Length=413, Percent_Identity=38.7409200968523, Blast_Score=265, Evalue=5e-71,
Organism=Escherichia coli, GI1789294, Length=391, Percent_Identity=71.8670076726343, Blast_Score=562, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI17508823, Length=411, Percent_Identity=42.8223844282238, Blast_Score=295, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6319857, Length=412, Percent_Identity=40.7766990291262, Blast_Score=286, Evalue=5e-78,
Organism=Drosophila melanogaster, GI17136394, Length=413, Percent_Identity=40.9200968523002, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=37.3493975903614, Blast_Score=247, Evalue=8e-66,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_SHEHH (B0TUC1)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673080.1
- ProteinModelPortal:   B0TUC1
- SMR:   B0TUC1
- GeneID:   5902988
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0846
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- ProtClustDB:   PRK00073
- BioCyc:   SHAL458817:SHAL_0846-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 40806; Mature: 40675

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 113-113 BINDING 146-146 BINDING 197-197 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILNMQDLELQGKRVLIREDLNVPVKDGVVTSDARLRASLPTIKLALEKGAAVMVMSHL
CCCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCHHEECCCEEEEEHHCCCEEEHHHHC
GRPTEGEFNAEFSMQPVVDYLTKALDCPVSLATEYLDGVEVAVGEVVVFENVRFNVGEKK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCEEEEECEEECCCCCC
NDEALAKKMAALCDVYVMDAFGTAHRAQASTHGVGLHAPIACAGPLLAGELAALGKALDN
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCC
PARPMVAIVGGSKVSTKLTVLESLSTKVDQLVVGGGIANTFVAAAGHQVGKSLYEADLIE
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EAKRLVANAQSRGGDIPVPTDVVVASEFSPTATATLKDVSTVSDTDMIFDIGPDSAEALA
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCEEEEECCCHHHHHH
EIIKNAGTVVWNGPVGVFEFDQFGEGTKRIAQAIAESNAFSIAGGGDTLAAVDKYGIADK
HHHHCCCEEEECCCCCEEECHHCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCHHH
VSYISTGGGAFLEFLEGKELPAVAMLESRGQ
HHHEECCCHHHHHHHCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
AILNMQDLELQGKRVLIREDLNVPVKDGVVTSDARLRASLPTIKLALEKGAAVMVMSHL
CCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCHHEECCCEEEEEHHCCCEEEHHHHC
GRPTEGEFNAEFSMQPVVDYLTKALDCPVSLATEYLDGVEVAVGEVVVFENVRFNVGEKK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCEEEEECEEECCCCCC
NDEALAKKMAALCDVYVMDAFGTAHRAQASTHGVGLHAPIACAGPLLAGELAALGKALDN
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCC
PARPMVAIVGGSKVSTKLTVLESLSTKVDQLVVGGGIANTFVAAAGHQVGKSLYEADLIE
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EAKRLVANAQSRGGDIPVPTDVVVASEFSPTATATLKDVSTVSDTDMIFDIGPDSAEALA
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCEEEEECCCHHHHHH
EIIKNAGTVVWNGPVGVFEFDQFGEGTKRIAQAIAESNAFSIAGGGDTLAAVDKYGIADK
HHHHCCCEEEECCCCCEEECHHCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCHHH
VSYISTGGGAFLEFLEGKELPAVAMLESRGQ
HHHEECCCHHHHHHHCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA