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The map label for this gene is ilvB [H]

Identifier: 167622782

GI number: 167622782

Start: 1045617

End: 1047347

Strand: Direct

Name: ilvB [H]

Synonym: Shal_0842

Alternate gene names: 167622782

Gene position: 1045617-1047347 (Clockwise)

Preceding gene: 167622781

Following gene: 167622784

Centisome position: 20.0

GC content: 49.28

Gene sequence:

>1731_bases
TTGAAAAGGACTGCAGCTTGGCTGACACGTTACGCCCTAGAGCAACTGAATATTAAGCATACCTTCGGCATTCCCGGTGT
GCATAACACTGAAATTTATGATGAGTTGGCTCAGTCGGATAGCATCGAACCCATATTGGTGACCCATGAGGGGCATGGTG
CCTTTATGGCCGATGCCGTCACCAGAAGTAGTGATAGCATGGGAACCTTACTTATTGTTCCTGCCGCTGGAGCCACCCAC
GCGGCCAGCGGCATTGCAGAAGCGTCGTTAGATGGCATTGCTATGCTAGTCATAAGTGGCGGCGTGAGAACCGATTCGAA
ATTTTCCTATCAACTTCATGATATGGATCAGCACAAAATGCTCGAAGCGATCACCAAAAAAACCTTCAAAGTCACTCGAC
ATCAAGATGTGGTCGCTACACTTTTTGAGGCGTACGATCTTGCCAATGATGGCGAACCCGGCCCTGTTTTTGTCGAGATC
CCGGTGAATCTTCAGCTCGACAGGGGCGAGATCTCAGACTTACCTATGTATAAAGCGCCGATAAAGTCATTGGCTCATGC
AATCGATGCTCAATTAGCACAAGCGGTAGCGTTATTAGCGAATGCTAAAAGCCCCGCCATATTTGTGGGCTGGGGCGGCG
TCGAGGCGAGTGATTGCACCGAACAGATCGCCGATTTACTTCACTCTCCTGTTGCGACAACACTGCAGGGGCTCAGTGCC
TTCTCAGCAAAGCATCCGCTTCATACCGGAATGGGATTTGGCCCCGCGGCAGTGCCCGCTGCGACCAATGCCTTTAAAGA
GTGTGATTGTCTGCTTGCCATCGGCACCCGTTTTGCTGAGATAGCAACAGGAAGCTTTGGGGTGATCGTGCCGGAAAACC
TAATCCACATCGATATTAACCCGAACGTGTTTAATGCCAATTACCCTGCTAAAGTCGCCATAGAGGGAGATGCTGGCGCT
GTTTTAGCCGCGCTATTGCAGATGCTCCAACAAACAAAGATCAGAGATAAAGATGCCAAAGCCATTAAACAGGCCATCAA
GCGCGATAAGCAGAGTTATATTGATGAGTGGCTAGCTCACGACAGTGGTGACAGAGTTAATCCTGCCGTTTTCTTTCAAC
ATCTTAGGCGGTCACTCGATGACGATGCCTTAGTGATCGCCGATGACGGAAATCATACCTTTTTAACCGCGGAGCTGATG
CCCTGTTATCGGGCGAAACACTTTTTTAGCCCTACAGATTTTAACTGCATGGGCTATGCGATCCCGGCGGTTATCGGTGC
TAAGCTTGCCAACCCTGAGAAACAGGTGGTTGGCATCATCGGTGACGGAGCATTTATGATGACTCAATCCGAGCTGTTTA
GTGCCTCCTCTCTTGGTATTGGCGCTATCTTTACCATCTTTAACGATGGTGAGCTTGCTCAGATCTCGCAGGCGCAAAAA
GTGCCTTACAATGCCGCAACCTGTACTGTCCTTCCCGAGTTTAAATTTAAGGCGTTAGTCGAGGCGACTGGTTGTCGCTA
CATTCGGCTACAGAACAATGACGAGGTGGCTGAACAGATATCCAAGGCCGTACTCTTAAGCGAGCAAGGAATACCTGTGG
TGCTGGATGTCAATATCGATTACAGCAAGAAGACCCGCTTTACCAAAGGGATCATCTCAACCAATCTAAAGCGAATGAAT
TTGCCGACCAAAGTCAGGATGATCAGCAGGGCACTTTGGCGAAGAATTTAA

Upstream 100 bases:

>100_bases
CTTGTATCTTAACTGCCCGCAATGCGGCGACTGCTATTGTTGGCAGTCATAGCTAGCCATAAATAACGAAAAATAAAAAA
AGAAATCTAAGGAGAGCACA

Downstream 100 bases:

>100_bases
AGCCCCATCCATGGTAAATCGTCATAAGTGTTCCAGACACAAAAAAACCGCCCTTGGGCGGTTTTTTTATTAGCGCTGAA
TAAACAGCGCTGAGTTAATC

Product: thiamine pyrophosphate protein central region

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MKRTAAWLTRYALEQLNIKHTFGIPGVHNTEIYDELAQSDSIEPILVTHEGHGAFMADAVTRSSDSMGTLLIVPAAGATH
AASGIAEASLDGIAMLVISGGVRTDSKFSYQLHDMDQHKMLEAITKKTFKVTRHQDVVATLFEAYDLANDGEPGPVFVEI
PVNLQLDRGEISDLPMYKAPIKSLAHAIDAQLAQAVALLANAKSPAIFVGWGGVEASDCTEQIADLLHSPVATTLQGLSA
FSAKHPLHTGMGFGPAAVPAATNAFKECDCLLAIGTRFAEIATGSFGVIVPENLIHIDINPNVFNANYPAKVAIEGDAGA
VLAALLQMLQQTKIRDKDAKAIKQAIKRDKQSYIDEWLAHDSGDRVNPAVFFQHLRRSLDDDALVIADDGNHTFLTAELM
PCYRAKHFFSPTDFNCMGYAIPAVIGAKLANPEKQVVGIIGDGAFMMTQSELFSASSLGIGAIFTIFNDGELAQISQAQK
VPYNAATCTVLPEFKFKALVEATGCRYIRLQNNDEVAEQISKAVLLSEQGIPVVLDVNIDYSKKTRFTKGIISTNLKRMN
LPTKVRMISRALWRRI

Sequences:

>Translated_576_residues
MKRTAAWLTRYALEQLNIKHTFGIPGVHNTEIYDELAQSDSIEPILVTHEGHGAFMADAVTRSSDSMGTLLIVPAAGATH
AASGIAEASLDGIAMLVISGGVRTDSKFSYQLHDMDQHKMLEAITKKTFKVTRHQDVVATLFEAYDLANDGEPGPVFVEI
PVNLQLDRGEISDLPMYKAPIKSLAHAIDAQLAQAVALLANAKSPAIFVGWGGVEASDCTEQIADLLHSPVATTLQGLSA
FSAKHPLHTGMGFGPAAVPAATNAFKECDCLLAIGTRFAEIATGSFGVIVPENLIHIDINPNVFNANYPAKVAIEGDAGA
VLAALLQMLQQTKIRDKDAKAIKQAIKRDKQSYIDEWLAHDSGDRVNPAVFFQHLRRSLDDDALVIADDGNHTFLTAELM
PCYRAKHFFSPTDFNCMGYAIPAVIGAKLANPEKQVVGIIGDGAFMMTQSELFSASSLGIGAIFTIFNDGELAQISQAQK
VPYNAATCTVLPEFKFKALVEATGCRYIRLQNNDEVAEQISKAVLLSEQGIPVVLDVNIDYSKKTRFTKGIISTNLKRMN
LPTKVRMISRALWRRI
>Mature_576_residues
MKRTAAWLTRYALEQLNIKHTFGIPGVHNTEIYDELAQSDSIEPILVTHEGHGAFMADAVTRSSDSMGTLLIVPAAGATH
AASGIAEASLDGIAMLVISGGVRTDSKFSYQLHDMDQHKMLEAITKKTFKVTRHQDVVATLFEAYDLANDGEPGPVFVEI
PVNLQLDRGEISDLPMYKAPIKSLAHAIDAQLAQAVALLANAKSPAIFVGWGGVEASDCTEQIADLLHSPVATTLQGLSA
FSAKHPLHTGMGFGPAAVPAATNAFKECDCLLAIGTRFAEIATGSFGVIVPENLIHIDINPNVFNANYPAKVAIEGDAGA
VLAALLQMLQQTKIRDKDAKAIKQAIKRDKQSYIDEWLAHDSGDRVNPAVFFQHLRRSLDDDALVIADDGNHTFLTAELM
PCYRAKHFFSPTDFNCMGYAIPAVIGAKLANPEKQVVGIIGDGAFMMTQSELFSASSLGIGAIFTIFNDGELAQISQAQK
VPYNAATCTVLPEFKFKALVEATGCRYIRLQNNDEVAEQISKAVLLSEQGIPVVLDVNIDYSKKTRFTKGIISTNLKRMN
LPTKVRMISRALWRRI

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=555, Percent_Identity=23.4234234234234, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI93004078, Length=555, Percent_Identity=22.5225225225225, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI87081685, Length=564, Percent_Identity=28.9007092198582, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1790104, Length=542, Percent_Identity=27.1217712177122, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1786717, Length=554, Percent_Identity=24.7292418772563, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787096, Length=540, Percent_Identity=23.5185185185185, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788716, Length=553, Percent_Identity=22.9656419529837, Blast_Score=96, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17531299, Length=480, Percent_Identity=25.625, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531301, Length=480, Percent_Identity=25.625, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17542570, Length=610, Percent_Identity=23.6065573770492, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=555, Percent_Identity=27.5675675675676, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6320816, Length=479, Percent_Identity=23.5908141962422, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=506, Percent_Identity=24.1106719367589, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922626, Length=474, Percent_Identity=22.1518987341772, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62434; Mature: 62434

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTAAWLTRYALEQLNIKHTFGIPGVHNTEIYDELAQSDSIEPILVTHEGHGAFMADAV
CCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEHHHH
TRSSDSMGTLLIVPAAGATHAASGIAEASLDGIAMLVISGGVRTDSKFSYQLHDMDQHKM
HCCCCCCCEEEEEECCCCHHHHHCHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCHHHH
LEAITKKTFKVTRHQDVVATLFEAYDLANDGEPGPVFVEIPVNLQLDRGEISDLPMYKAP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECEEEECCCCCCCCCCHHHH
IKSLAHAIDAQLAQAVALLANAKSPAIFVGWGGVEASDCTEQIADLLHSPVATTLQGLSA
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHH
FSAKHPLHTGMGFGPAAVPAATNAFKECDCLLAIGTRFAEIATGSFGVIVPENLIHIDIN
HHCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECC
PNVFNANYPAKVAIEGDAGAVLAALLQMLQQTKIRDKDAKAIKQAIKRDKQSYIDEWLAH
CCEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
DSGDRVNPAVFFQHLRRSLDDDALVIADDGNHTFLTAELMPCYRAKHFFSPTDFNCMGYA
CCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEHHHHHHHHHCCCCCCCCHHHHH
IPAVIGAKLANPEKQVVGIIGDGAFMMTQSELFSASSLGIGAIFTIFNDGELAQISQAQK
HHHHHHHHCCCCCHHEEEEEECCEEEEEHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHC
VPYNAATCTVLPEFKFKALVEATGCRYIRLQNNDEVAEQISKAVLLSEQGIPVVLDVNID
CCCCCEEEEEECCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCC
YSKKTRFTKGIISTNLKRMNLPTKVRMISRALWRRI
CCCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRTAAWLTRYALEQLNIKHTFGIPGVHNTEIYDELAQSDSIEPILVTHEGHGAFMADAV
CCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEHHHH
TRSSDSMGTLLIVPAAGATHAASGIAEASLDGIAMLVISGGVRTDSKFSYQLHDMDQHKM
HCCCCCCCEEEEEECCCCHHHHHCHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCHHHH
LEAITKKTFKVTRHQDVVATLFEAYDLANDGEPGPVFVEIPVNLQLDRGEISDLPMYKAP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECEEEECCCCCCCCCCHHHH
IKSLAHAIDAQLAQAVALLANAKSPAIFVGWGGVEASDCTEQIADLLHSPVATTLQGLSA
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHH
FSAKHPLHTGMGFGPAAVPAATNAFKECDCLLAIGTRFAEIATGSFGVIVPENLIHIDIN
HHCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECC
PNVFNANYPAKVAIEGDAGAVLAALLQMLQQTKIRDKDAKAIKQAIKRDKQSYIDEWLAH
CCEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
DSGDRVNPAVFFQHLRRSLDDDALVIADDGNHTFLTAELMPCYRAKHFFSPTDFNCMGYA
CCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEHHHHHHHHHCCCCCCCCHHHHH
IPAVIGAKLANPEKQVVGIIGDGAFMMTQSELFSASSLGIGAIFTIFNDGELAQISQAQK
HHHHHHHHCCCCCHHEEEEEECCEEEEEHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHC
VPYNAATCTVLPEFKFKALVEATGCRYIRLQNNDEVAEQISKAVLLSEQGIPVVLDVNID
CCCCCEEEEEECCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCC
YSKKTRFTKGIISTNLKRMNLPTKVRMISRALWRRI
CCCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626 [H]