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The map label for this gene is ahpF [H]

Identifier: 167622740

GI number: 167622740

Start: 995018

End: 996592

Strand: Direct

Name: ahpF [H]

Synonym: Shal_0800

Alternate gene names: 167622740

Gene position: 995018-996592 (Clockwise)

Preceding gene: 167622739

Following gene: 167622741

Centisome position: 19.04

GC content: 47.87

Gene sequence:

>1575_bases
ATGTTAGATGCAAACGTAAAGAATCAACTGAGCACTTATTTGCAAAACCTCAAGCATCCAGTTGAGCTGGTTGTATCTAT
TGATGACAGTAAAAAAGCCCAAGAGTTAAAGTCACTTGCTGCAGATATCGATAGCTTGTCTCCACTCATCTCAGTAATAG
ATCAAGTGGGTGAGCGTACTCCTTCGATGTCAGTCATCAATCCACAATTGAATAGTCAGATCACCTTTGCAGGCCTGCCT
ATGGGTCATGAGTTTACCTCCTTGGTGCTTGCGCTTCTGCATAGCGGCGGTCACCCAATCAAGCTAGATAGCGACGTGAT
TGAACAAATTCGCCAGCTACCGGGTGAGTATCGTTTCGAGACCTATGTGTCTTTAAGCTGTCAAACCTGTCCTGAAGTGG
TTCAAGCGCTGAATATGATGGCGGCGATTAACCCGAACATTACTAACGTGATGATTGATGGTTCACTATTCCAAGATGAA
GTGAATGAGCGCAACATCATGGCGGTCCCTTCTGTGTACCTCAATGGCGAGCCGTTCTCAATGGGCGCCATTAGCACGGT
TGAGATCTTAAACAAGCTTGACGTCAATGCGGCGGGTCGAAAAGCTGAGCAGCTAAACGAGAAAGAAGCGTTTGATGTAT
TAGTGGTTGGCGGTGGCCCAGCAGGCGCATCGGCAGCAATTTATGCGGCACGTAAAGGTCTACGTACTGGCATAGTTGCC
GATAAGTTTGGTGGTCAAGTCGCTGAAACCGTGGGTATCGAAAACTTCATTTCAGTTAAGGCGACAGAAGGCCCTAAGCT
GGTGGCTAATCTTGAGGCCCACGTACATGAGTACGATGTTGATATTATGGATAATCAACGCGCGTTGAGCCTAAAGTCGG
GTGAGCTGTTTGAGTTAGCGCTTGAAAGCGGCGCGGTGCTGCGCAGTAAAACAATATTACTGGCAACGGGGGCACGCTGG
CGTGAAATGAACGTACCGGGTGAGCAAGAATACCGTGGTAGTGGTGTGGCGTACTGCCCACATTGTGACGGCCCACTGTT
TAAAGGTAAGCGTGTTGCGGTTATTGGCGGTGGCAACTCTGGTATTGAAGCGGCAATTGATTTAGCGAATATTGTTGAGC
ACGTTACCGTACTTGAATTTGATAAAACATTGCGTGCCGATGAAGTGCTGCAGCGTAAAGCAAAGTCGATGGGCAACATT
GATATCATTACCGAGGCGATGACCACTGAGGTGACTGGCGATGGTAAGCGTGTTAACGGCCTTAACTATACCGACAGAGC
CACAGGCGAGAGCCATCATGTTGACCTGGCAGGTATCTTTGTACAGATTGGTTTGGTACCAAACTCTGAGTGGCTAAAAG
ACACCATAGATATGACAGCGCGCGGTGAGATTGTTGTCGATGCAAAAGGCGAAACTTCATTACCTGGGGTATTTGCTGCG
GGTGATGTGACAAACTCAGCTTATAAGCAGATTATTATTGCCATGGGGAGTGGTGCAACGGCATCACTGGGTGCATTTGA
CTACTTAATTCGTCACGATGTCGATGAGTCTAGCCGAGATAATGTTGCAGCCTAA

Upstream 100 bases:

>100_bases
AGACCACTTTTTATTTTTTAGTGAGCATTTAGTTGCTTGTGGCTAATGCTGCCTGCTGCTCACCTATATCAGATTAGCTT
TCACATATTTAGGAGTCATC

Downstream 100 bases:

>100_bases
AGGGATTACTCGTTAATATAAAGCCAGCAGATGCTGGCTTTTTTATTGAGCTAGTCACAGAATAATAATGATAAGTGAAG
GGATTCCTTTGAAGTCATAT

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MLDANVKNQLSTYLQNLKHPVELVVSIDDSKKAQELKSLAADIDSLSPLISVIDQVGERTPSMSVINPQLNSQITFAGLP
MGHEFTSLVLALLHSGGHPIKLDSDVIEQIRQLPGEYRFETYVSLSCQTCPEVVQALNMMAAINPNITNVMIDGSLFQDE
VNERNIMAVPSVYLNGEPFSMGAISTVEILNKLDVNAAGRKAEQLNEKEAFDVLVVGGGPAGASAAIYAARKGLRTGIVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVHEYDVDIMDNQRALSLKSGELFELALESGAVLRSKTILLATGARW
REMNVPGEQEYRGSGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDKTLRADEVLQRKAKSMGNI
DIITEAMTTEVTGDGKRVNGLNYTDRATGESHHVDLAGIFVQIGLVPNSEWLKDTIDMTARGEIVVDAKGETSLPGVFAA
GDVTNSAYKQIIIAMGSGATASLGAFDYLIRHDVDESSRDNVAA

Sequences:

>Translated_524_residues
MLDANVKNQLSTYLQNLKHPVELVVSIDDSKKAQELKSLAADIDSLSPLISVIDQVGERTPSMSVINPQLNSQITFAGLP
MGHEFTSLVLALLHSGGHPIKLDSDVIEQIRQLPGEYRFETYVSLSCQTCPEVVQALNMMAAINPNITNVMIDGSLFQDE
VNERNIMAVPSVYLNGEPFSMGAISTVEILNKLDVNAAGRKAEQLNEKEAFDVLVVGGGPAGASAAIYAARKGLRTGIVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVHEYDVDIMDNQRALSLKSGELFELALESGAVLRSKTILLATGARW
REMNVPGEQEYRGSGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDKTLRADEVLQRKAKSMGNI
DIITEAMTTEVTGDGKRVNGLNYTDRATGESHHVDLAGIFVQIGLVPNSEWLKDTIDMTARGEIVVDAKGETSLPGVFAA
GDVTNSAYKQIIIAMGSGATASLGAFDYLIRHDVDESSRDNVAA
>Mature_524_residues
MLDANVKNQLSTYLQNLKHPVELVVSIDDSKKAQELKSLAADIDSLSPLISVIDQVGERTPSMSVINPQLNSQITFAGLP
MGHEFTSLVLALLHSGGHPIKLDSDVIEQIRQLPGEYRFETYVSLSCQTCPEVVQALNMMAAINPNITNVMIDGSLFQDE
VNERNIMAVPSVYLNGEPFSMGAISTVEILNKLDVNAAGRKAEQLNEKEAFDVLVVGGGPAGASAAIYAARKGLRTGIVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVHEYDVDIMDNQRALSLKSGELFELALESGAVLRSKTILLATGARW
REMNVPGEQEYRGSGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDKTLRADEVLQRKAKSMGNI
DIITEAMTTEVTGDGKRVNGLNYTDRATGESHHVDLAGIFVQIGLVPNSEWLKDTIDMTARGEIVVDAKGETSLPGVFAA
GDVTNSAYKQIIIAMGSGATASLGAFDYLIRHDVDESSRDNVAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=517, Percent_Identity=57.0599613152805, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1787114, Length=309, Percent_Identity=33.9805825242718, Blast_Score=160, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321898, Length=334, Percent_Identity=32.3353293413174, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320560, Length=310, Percent_Identity=34.1935483870968, Blast_Score=159, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56147; Mature: 56147

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDANVKNQLSTYLQNLKHPVELVVSIDDSKKAQELKSLAADIDSLSPLISVIDQVGERT
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSMSVINPQLNSQITFAGLPMGHEFTSLVLALLHSGGHPIKLDSDVIEQIRQLPGEYRFE
CCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCEEE
TYVSLSCQTCPEVVQALNMMAAINPNITNVMIDGSLFQDEVNERNIMAVPSVYLNGEPFS
EEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCEEEECEEEECCCCCC
MGAISTVEILNKLDVNAAGRKAEQLNEKEAFDVLVVGGGPAGASAAIYAARKGLRTGIVA
CCCHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCH
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVHEYDVDIMDNQRALSLKSGELFELA
HHCCCHHHHHHCCCCEEEEEECCCCEEEEHHHHHHEEECCEEECCCEEEEECCCCEEEEH
LESGAVLRSKTILLATGARWREMNVPGEQEYRGSGVAYCPHCDGPLFKGKRVAVIGGGNS
HHCCCEEEECEEEEEECCCEEECCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCC
GIEAAIDLANIVEHVTVLEFDKTLRADEVLQRKAKSMGNIDIITEAMTTEVTGDGKRVNG
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEHHHEEEECCCCCEECC
LNYTDRATGESHHVDLAGIFVQIGLVPNSEWLKDTIDMTARGEIVVDAKGETSLPGVFAA
CCCCCCCCCCCCEEEEEEEEEEEEECCCHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEE
GDVTNSAYKQIIIAMGSGATASLGAFDYLIRHDVDESSRDNVAA
CCCCHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLDANVKNQLSTYLQNLKHPVELVVSIDDSKKAQELKSLAADIDSLSPLISVIDQVGERT
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSMSVINPQLNSQITFAGLPMGHEFTSLVLALLHSGGHPIKLDSDVIEQIRQLPGEYRFE
CCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCEEE
TYVSLSCQTCPEVVQALNMMAAINPNITNVMIDGSLFQDEVNERNIMAVPSVYLNGEPFS
EEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCEEEECEEEECCCCCC
MGAISTVEILNKLDVNAAGRKAEQLNEKEAFDVLVVGGGPAGASAAIYAARKGLRTGIVA
CCCHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCH
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVHEYDVDIMDNQRALSLKSGELFELA
HHCCCHHHHHHCCCCEEEEEECCCCEEEEHHHHHHEEECCEEECCCEEEEECCCCEEEEH
LESGAVLRSKTILLATGARWREMNVPGEQEYRGSGVAYCPHCDGPLFKGKRVAVIGGGNS
HHCCCEEEECEEEEEECCCEEECCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCC
GIEAAIDLANIVEHVTVLEFDKTLRADEVLQRKAKSMGNIDIITEAMTTEVTGDGKRVNG
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEHHHEEEECCCCCEECC
LNYTDRATGESHHVDLAGIFVQIGLVPNSEWLKDTIDMTARGEIVVDAKGETSLPGVFAA
CCCCCCCCCCCCEEEEEEEEEEEEECCCHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEE
GDVTNSAYKQIIIAMGSGATASLGAFDYLIRHDVDESSRDNVAA
CCCCHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]