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The map label for this gene is panB

Identifier: 167622690

GI number: 167622690

Start: 930758

End: 931552

Strand: Reverse

Name: panB

Synonym: Shal_0750

Alternate gene names: 167622690

Gene position: 931552-930758 (Counterclockwise)

Preceding gene: 167622691

Following gene: 167622689

Centisome position: 17.82

GC content: 49.18

Gene sequence:

>795_bases
ATGTCCAAAATTACAAGTTCTACACTGCGTACCTTTAAGCAAGAAGGTAAGAAATTCACTGCATTAACGGCCTATGATGC
CAGCTTTGCCAGCGCGTTCGATAGCGAAGGTATTGACGTGTTATTAGTCGGTGACTCAATGGGAATGGTACTACAAGGAC
ATAGCGACACTCTGCCGGTCACGGTTGACGATATTGCCTACCATACCCGTTGTGTACGTCGTGGTATTGAGCATTCACTG
TTGATCGCCGATATGCCATTTATGAGCTATGCCACACCAGAGCAGACCATGGTTAACGCCACCACGCTAATGCAAGCAGG
CGCAAGCATGGTCAAGGTTGAGGGCGGACATTGGCTACTAGAAAGTGTCGCCATGCTGACTGAACGCGGTATTCCTGTCT
GTGCCCATTTAGGCCTAACGCCACAATCTGTTCATGTTTTTGGTGGCTTTAAGGTACAGGGTCGTGACGCCGATAATGCA
CAGCGCATCCTTGATGAAGCTAAGGCGTTAGAAGCCGCGGGAGCACAACTACTGGTAGTTGAATGCATACCCGCATCGTT
AGCAAAAGCGATTACCCAAGCCCTCACGATACCTGTTATCGGTATTGGCGCAGGTAAAGACACCGATGGTCAGATCTTAG
TGATGCACGATGTGCTTGGGATCTCCAGCGGCTATATTCCCCGTTTTTCTAAAAACTACCTCAAGCAGACTGGCGAAATC
CGTGCAGCCGTTCGTGCCTATATTGACGAAGTGGCTCAAGGTGTTTTCCCAGGCGATGAGCATACCTTCAACTAA

Upstream 100 bases:

>100_bases
GCCAGTATCCGTTTAAAAAAACATTCGACACCGAGAACATTGAAGTTGAGCCTTTATTGGCATATAACAACGTCACAAAT
TGGGTTTTAAAATTACCATT

Downstream 100 bases:

>100_bases
TGATATGCTCTCGATCCCCTGATTGAGAGCGCACTTTCAAATTAGTGTTAGAGCAATAAAATCATGATCACGACTCAATC
TATTTCTGATATCCGTGCCC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSKITSSTLRTFKQEGKKFTALTAYDASFASAFDSEGIDVLLVGDSMGMVLQGHSDTLPVTVDDIAYHTRCVRRGIEHSL
LIADMPFMSYATPEQTMVNATTLMQAGASMVKVEGGHWLLESVAMLTERGIPVCAHLGLTPQSVHVFGGFKVQGRDADNA
QRILDEAKALEAAGAQLLVVECIPASLAKAITQALTIPVIGIGAGKDTDGQILVMHDVLGISSGYIPRFSKNYLKQTGEI
RAAVRAYIDEVAQGVFPGDEHTFN

Sequences:

>Translated_264_residues
MSKITSSTLRTFKQEGKKFTALTAYDASFASAFDSEGIDVLLVGDSMGMVLQGHSDTLPVTVDDIAYHTRCVRRGIEHSL
LIADMPFMSYATPEQTMVNATTLMQAGASMVKVEGGHWLLESVAMLTERGIPVCAHLGLTPQSVHVFGGFKVQGRDADNA
QRILDEAKALEAAGAQLLVVECIPASLAKAITQALTIPVIGIGAGKDTDGQILVMHDVLGISSGYIPRFSKNYLKQTGEI
RAAVRAYIDEVAQGVFPGDEHTFN
>Mature_263_residues
SKITSSTLRTFKQEGKKFTALTAYDASFASAFDSEGIDVLLVGDSMGMVLQGHSDTLPVTVDDIAYHTRCVRRGIEHSLL
IADMPFMSYATPEQTMVNATTLMQAGASMVKVEGGHWLLESVAMLTERGIPVCAHLGLTPQSVHVFGGFKVQGRDADNAQ
RILDEAKALEAAGAQLLVVECIPASLAKAITQALTIPVIGIGAGKDTDGQILVMHDVLGISSGYIPRFSKNYLKQTGEIR
AAVRAYIDEVAQGVFPGDEHTFN

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=264, Percent_Identity=64.3939393939394, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319653, Length=258, Percent_Identity=36.4341085271318, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_SHEHH (B0TTI2)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672984.1
- ProteinModelPortal:   B0TTI2
- SMR:   B0TTI2
- GeneID:   5905472
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0750
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   SHAL458817:SHAL_0750-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28273; Mature: 28142

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 84-84 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKITSSTLRTFKQEGKKFTALTAYDASFASAFDSEGIDVLLVGDSMGMVLQGHSDTLPV
CCCCHHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCE
TVDDIAYHTRCVRRGIEHSLLIADMPFMSYATPEQTMVNATTLMQAGASMVKVEGGHWLL
EHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHH
ESVAMLTERGIPVCAHLGLTPQSVHVFGGFKVQGRDADNAQRILDEAKALEAAGAQLLVV
HHHHHHHHCCCCEEEECCCCCCEEEEEECEEEECCCCCHHHHHHHHHHHHHHCCCEEEEE
ECIPASLAKAITQALTIPVIGIGAGKDTDGQILVMHDVLGISSGYIPRFSKNYLKQTGEI
ECCCHHHHHHHHHHHHHCEEEECCCCCCCCCEEEEEEHHCCCCCCCCCHHHHHHHHHHHH
RAAVRAYIDEVAQGVFPGDEHTFN
HHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKITSSTLRTFKQEGKKFTALTAYDASFASAFDSEGIDVLLVGDSMGMVLQGHSDTLPV
CCCHHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCE
TVDDIAYHTRCVRRGIEHSLLIADMPFMSYATPEQTMVNATTLMQAGASMVKVEGGHWLL
EHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHH
ESVAMLTERGIPVCAHLGLTPQSVHVFGGFKVQGRDADNAQRILDEAKALEAAGAQLLVV
HHHHHHHHCCCCEEEECCCCCCEEEEEECEEEECCCCCHHHHHHHHHHHHHHCCCEEEEE
ECIPASLAKAITQALTIPVIGIGAGKDTDGQILVMHDVLGISSGYIPRFSKNYLKQTGEI
ECCCHHHHHHHHHHHHHCEEEECCCCCCCCCEEEEEEHHCCCCCCCCCHHHHHHHHHHHH
RAAVRAYIDEVAQGVFPGDEHTFN
HHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA