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The map label for this gene is mdeA [H]

Identifier: 167622677

GI number: 167622677

Start: 916105

End: 917385

Strand: Direct

Name: mdeA [H]

Synonym: Shal_0737

Alternate gene names: 167622677

Gene position: 916105-917385 (Clockwise)

Preceding gene: 167622676

Following gene: 167622678

Centisome position: 17.53

GC content: 51.52

Gene sequence:

>1281_bases
ATGAAACTCGAATCTTTAGCGCTGCACCACGGATATGAATCGGAATCGACCACTAAGGCTGCAGCCGTTCCTATTTATCA
GACCACCTCCTATACCTTCGATAACACTCAACATGGCGCCGATCTGTTCGATCTGAAAGTACCGGGTAATATTTACACTC
GCATCATGAACCCCACCACAGATGTGCTCGAGCAGCGCCTTGCGGCGATAGAAGGCGGCATAGGAGCACTCGCACTTGCA
TCGGGTATGGCGGCGATTACCTATGCGATTCAAGCATTGACTCAGGTGGGCGACAATATCGTTAGTACCAGTCAGCTCTA
TGGTGGCACCTATAATTTGTTTGCTCATACTTTGCCGCGCCAAGGCGTAGAGGTGCGTATGGCTGCATTCGATGATTTCG
AACAGCTCGATGCCTTGATTGACGATAACACTAAGGCGCTGTTTTGTGAGTCTATCGGTAACCCCGCGGGCAATATTGTC
GACCTACAGCGTTTAGCCGAAATCGCTCACAAGCACGGCGTTCCTCTGATTGTTGATAATACGGTGGCGACTCCGGTGCT
GTGTAAGCCATTTGAACATGGCGCCGACATTGTTATCCATTCGCTGACCAAATATATTGGCGGCCATGGCACCACCATAG
GTGGCGCGATAATCGATAGCGGCAAGTTTGACTGGGCGGCAGAGCCTAAGCGTTTTGCACTGCTCAATGAGCCGGATCCC
TCTTACCATGGCGTGATTTATACCCAAGCCTTCGGCCCAGCCGCCTTTATCGGTCGCTGCCGCGTGGTGCCACTGCGTAA
TACCGGCGCAGCGTTATCGCCTCAAAGTGCCTTCTTGCTGTTACAAGGGTTAGAAACGCTAGCATTAAGAATGGAGCGCC
ATTGTGAAAATGCCTTGGCATTGGCCCAGTATTTAGAGTCTCATCCTAAGGTGAGCTGGGTTAATTACGCCGCGCTGGAA
AATAGCCCCTTTCAAGATAACTGCCACAAGATCACTGGCGGTAAAGCGTCGGGGATCATCAGCTTCGGCATAAAAGCAGA
TGATGGCAAGGTCGCTGGTGGCCGTTTTATCGATGCGCTGCAGATGATTTTAAGACTGGTCAATATCGGCGATGCTAAGT
CGTTAGCCTGTCACCCAGCGTCGACGACTCACAGGCAGCTCGATGCCAGTGAGCTTGCTAAGGCGGGCGTCAGTGAAGAC
TTAGTACGCATCTCTGTGGGGATTGAGCATATCGATGACATTATTAGCGACGTGGCGCAGGCACTGGATAAAGCGGTTTA
A

Upstream 100 bases:

>100_bases
GCCTCTATCGGCCATCTAGTGAGTGGTGTATTTTTATTTAGACTTCTAGATGGCCAGCTGTTATGTTGCTATTTCAATCA
ATGAAATAGGATATGACGCG

Downstream 100 bases:

>100_bases
GACTAGCGAACCCTATCAGATGCAGCCAAAGCACTAAGCTGGTTTGGCTGCATTTACTTTTTTGCTTGTCCGTCTTAGCC
CATCGTCGATGACGGCATAA

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKLESLALHHGYESESTTKAAAVPIYQTTSYTFDNTQHGADLFDLKVPGNIYTRIMNPTTDVLEQRLAAIEGGIGALALA
SGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGVEVRMAAFDDFEQLDALIDDNTKALFCESIGNPAGNIV
DLQRLAEIAHKHGVPLIVDNTVATPVLCKPFEHGADIVIHSLTKYIGGHGTTIGGAIIDSGKFDWAAEPKRFALLNEPDP
SYHGVIYTQAFGPAAFIGRCRVVPLRNTGAALSPQSAFLLLQGLETLALRMERHCENALALAQYLESHPKVSWVNYAALE
NSPFQDNCHKITGGKASGIISFGIKADDGKVAGGRFIDALQMILRLVNIGDAKSLACHPASTTHRQLDASELAKAGVSED
LVRISVGIEHIDDIISDVAQALDKAV

Sequences:

>Translated_426_residues
MKLESLALHHGYESESTTKAAAVPIYQTTSYTFDNTQHGADLFDLKVPGNIYTRIMNPTTDVLEQRLAAIEGGIGALALA
SGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGVEVRMAAFDDFEQLDALIDDNTKALFCESIGNPAGNIV
DLQRLAEIAHKHGVPLIVDNTVATPVLCKPFEHGADIVIHSLTKYIGGHGTTIGGAIIDSGKFDWAAEPKRFALLNEPDP
SYHGVIYTQAFGPAAFIGRCRVVPLRNTGAALSPQSAFLLLQGLETLALRMERHCENALALAQYLESHPKVSWVNYAALE
NSPFQDNCHKITGGKASGIISFGIKADDGKVAGGRFIDALQMILRLVNIGDAKSLACHPASTTHRQLDASELAKAGVSED
LVRISVGIEHIDDIISDVAQALDKAV
>Mature_426_residues
MKLESLALHHGYESESTTKAAAVPIYQTTSYTFDNTQHGADLFDLKVPGNIYTRIMNPTTDVLEQRLAAIEGGIGALALA
SGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGVEVRMAAFDDFEQLDALIDDNTKALFCESIGNPAGNIV
DLQRLAEIAHKHGVPLIVDNTVATPVLCKPFEHGADIVIHSLTKYIGGHGTTIGGAIIDSGKFDWAAEPKRFALLNEPDP
SYHGVIYTQAFGPAAFIGRCRVVPLRNTGAALSPQSAFLLLQGLETLALRMERHCENALALAQYLESHPKVSWVNYAALE
NSPFQDNCHKITGGKASGIISFGIKADDGKVAGGRFIDALQMILRLVNIGDAKSLACHPASTTHRQLDASELAKAGVSED
LVRISVGIEHIDDIISDVAQALDKAV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=422, Percent_Identity=32.4644549763033, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI299473759, Length=422, Percent_Identity=28.9099526066351, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI25453487, Length=421, Percent_Identity=28.7410926365796, Blast_Score=122, Evalue=8e-28,
Organism=Escherichia coli, GI1790375, Length=429, Percent_Identity=30.0699300699301, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1789383, Length=423, Percent_Identity=24.3498817966903, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71980708, Length=419, Percent_Identity=32.4582338902148, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17538055, Length=425, Percent_Identity=34.3529411764706, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17560056, Length=425, Percent_Identity=34.5882352941176, Blast_Score=189, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=48.8425925925926, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6319307, Length=382, Percent_Identity=33.5078534031414, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=447, Percent_Identity=25.503355704698, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=340, Percent_Identity=27.3529411764706, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=432, Percent_Identity=25.9259259259259, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922554, Length=429, Percent_Identity=32.4009324009324, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45641; Mature: 45641

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLESLALHHGYESESTTKAAAVPIYQTTSYTFDNTQHGADLFDLKVPGNIYTRIMNPTT
CCCHHHHHHCCCCCCCCCCCEEEEEEECCCEECCCCCCCCCEEEEECCCHHEEEECCCHH
DVLEQRLAAIEGGIGALALASGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCC
QGVEVRMAAFDDFEQLDALIDDNTKALFCESIGNPAGNIVDLQRLAEIAHKHGVPLIVDN
CCCEEEEEHHCCHHHHHHHHCCCCCEEEEHHCCCCCCCHHHHHHHHHHHHHCCCCEEECC
TVATPVLCKPFEHGADIVIHSLTKYIGGHGTTIGGAIIDSGKFDWAAEPKRFALLNEPDP
CCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEECCEEEECCCCCCCCCCCEEEEECCCCC
SYHGVIYTQAFGPAAFIGRCRVVPLRNTGAALSPQSAFLLLQGLETLALRMERHCENALA
CCCEEEEEECCCCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAQYLESHPKVSWVNYAALENSPFQDNCHKITGGKASGIISFGIKADDGKVAGGRFIDAL
HHHHHHHCCCEEEEEEEECCCCCCCCCCCEECCCCCCCEEEEEEECCCCCCCCHHHHHHH
QMILRLVNIGDAKSLACHPASTTHRQLDASELAKAGVSEDLVRISVGIEHIDDIISDVAQ
HHHHHHHCCCCCCCEEECCCCCHHHHCCHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHH
ALDKAV
HHHHCC
>Mature Secondary Structure
MKLESLALHHGYESESTTKAAAVPIYQTTSYTFDNTQHGADLFDLKVPGNIYTRIMNPTT
CCCHHHHHHCCCCCCCCCCCEEEEEEECCCEECCCCCCCCCEEEEECCCHHEEEECCCHH
DVLEQRLAAIEGGIGALALASGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCC
QGVEVRMAAFDDFEQLDALIDDNTKALFCESIGNPAGNIVDLQRLAEIAHKHGVPLIVDN
CCCEEEEEHHCCHHHHHHHHCCCCCEEEEHHCCCCCCCHHHHHHHHHHHHHCCCCEEECC
TVATPVLCKPFEHGADIVIHSLTKYIGGHGTTIGGAIIDSGKFDWAAEPKRFALLNEPDP
CCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEECCEEEECCCCCCCCCCCEEEEECCCCC
SYHGVIYTQAFGPAAFIGRCRVVPLRNTGAALSPQSAFLLLQGLETLALRMERHCENALA
CCCEEEEEECCCCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAQYLESHPKVSWVNYAALENSPFQDNCHKITGGKASGIISFGIKADDGKVAGGRFIDAL
HHHHHHHCCCEEEEEEEECCCCCCCCCCCEECCCCCCCEEEEEEECCCCCCCCHHHHHHH
QMILRLVNIGDAKSLACHPASTTHRQLDASELAKAGVSEDLVRISVGIEHIDDIISDVAQ
HHHHHHHCCCCCCCEEECCCCCHHHHCCHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHH
ALDKAV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]