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The map label for this gene is rpfG [H]

Identifier: 167622661

GI number: 167622661

Start: 896374

End: 897393

Strand: Direct

Name: rpfG [H]

Synonym: Shal_0721

Alternate gene names: 167622661

Gene position: 896374-897393 (Clockwise)

Preceding gene: 167622660

Following gene: 167622664

Centisome position: 17.15

GC content: 48.53

Gene sequence:

>1020_bases
ATGGATAAGGCCACCATTTTAGTTGTGGATGATACCCCCGAGAATATTGATATTTTGGTTGGGATCTTAGGTGGGGACTA
CCGAGTTAAGGTTGCGATTGATGGCCCCAAAGCCCTTGCATTGGCGAGTAAAACCACACCGGATCTTATTCTTCTGGATG
TGATGATGCCGGGAATGAATGGCTATGAGGTATGTAAGCGACTAAAGCAAGAACCATTAACTTGCCATATTCCCGTCATC
TTTGTCACCGCCCTTAGCGATGTCGCCGATGAGACGCAAGGGTTTGAGCTTGGCGCCGTCGATTACATTACAAAACCCGT
CAGTGCGCCTGTTGTGAAGGCGAGGGTAAAAACCCATTTGGCTCTCTACGACCAAAAATGTCTACTCGAGCAGGAAGTTA
AAGTGCGAACTAAAGAGCTCGAAGAAACACGTTTCGAGATCATTCGTCGTCTGGGCCGCGCAGCAGAATATAAAGATAAC
GAGACTGGCTTACATGTTGTCAGAATGAGTCACTATGCGCGCTTGTTGGCGGTTCAATCGGGTTTACCTGAATACTATTG
CGAGCTGATTTACAATGCTGCGCCTATGCATGATATCGGTAAAATAGGCACACCAGACTCTATTTTAAAAAAGCCAGCTA
AGCTCGATGCTACAGAGTGGGTGGAGATGCAGCTTCACGCCGAAATCGGCGCCGAGATCATCGGTGAGCACAATGATCCA
TTGCTGGAGATGGCGCGTCGAATCGCCTTAACACATCACGAGAAATGGGATGGCAGCGGTTACCCCAATGGGCTGTCGGC
TGAGGAGATCCCAATAGAGGGTCGGATAGTGGCGATTGCCGATGTGTTTGACGCCTTAACTTCGATTCGCCCCTATAAGA
AAGCGTGGACAGTGGAGGCGACCATGACCTTAATCGAGAGTGAAGCTGGAAAGCAGTTTGACCCAAAGTTAGTGCAGCAT
TTTAAGCAGATTGTTGATGAGCTGACTAAAGTTCGCGATGCTCACAATGAGACGGGTTAG

Upstream 100 bases:

>100_bases
AAAAATATCATAGGTAACCTAAGCTGTTTTAGTGCCAAGCATAATAAGCGAGCAGCTATACTTGATTGCTAGCGTGAATA
CTAAAAGGAGAGATGGTTTA

Downstream 100 bases:

>100_bases
ATTTAGTCTTGATTAGTCGGGATAAACAAAAAGGCGCATAAAGCGCCTTTTCTATTACGAACGAATTGGGGGCTGGATTA
ATCTTGCTCTTCTAGCGAGT

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MDKATILVVDDTPENIDILVGILGGDYRVKVAIDGPKALALASKTTPDLILLDVMMPGMNGYEVCKRLKQEPLTCHIPVI
FVTALSDVADETQGFELGAVDYITKPVSAPVVKARVKTHLALYDQKCLLEQEVKVRTKELEETRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARLLAVQSGLPEYYCELIYNAAPMHDIGKIGTPDSILKKPAKLDATEWVEMQLHAEIGAEIIGEHNDP
LLEMARRIALTHHEKWDGSGYPNGLSAEEIPIEGRIVAIADVFDALTSIRPYKKAWTVEATMTLIESEAGKQFDPKLVQH
FKQIVDELTKVRDAHNETG

Sequences:

>Translated_339_residues
MDKATILVVDDTPENIDILVGILGGDYRVKVAIDGPKALALASKTTPDLILLDVMMPGMNGYEVCKRLKQEPLTCHIPVI
FVTALSDVADETQGFELGAVDYITKPVSAPVVKARVKTHLALYDQKCLLEQEVKVRTKELEETRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARLLAVQSGLPEYYCELIYNAAPMHDIGKIGTPDSILKKPAKLDATEWVEMQLHAEIGAEIIGEHNDP
LLEMARRIALTHHEKWDGSGYPNGLSAEEIPIEGRIVAIADVFDALTSIRPYKKAWTVEATMTLIESEAGKQFDPKLVQH
FKQIVDELTKVRDAHNETG
>Mature_339_residues
MDKATILVVDDTPENIDILVGILGGDYRVKVAIDGPKALALASKTTPDLILLDVMMPGMNGYEVCKRLKQEPLTCHIPVI
FVTALSDVADETQGFELGAVDYITKPVSAPVVKARVKTHLALYDQKCLLEQEVKVRTKELEETRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARLLAVQSGLPEYYCELIYNAAPMHDIGKIGTPDSILKKPAKLDATEWVEMQLHAEIGAEIIGEHNDP
LLEMARRIALTHHEKWDGSGYPNGLSAEEIPIEGRIVAIADVFDALTSIRPYKKAWTVEATMTLIESEAGKQFDPKLVQH
FKQIVDELTKVRDAHNETG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=146, Percent_Identity=35.6164383561644, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790860, Length=123, Percent_Identity=39.0243902439024, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=39.8230088495575, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693157, Length=126, Percent_Identity=39.6825396825397, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=35.3448275862069, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786784, Length=116, Percent_Identity=32.7586206896552, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790437, Length=121, Percent_Identity=36.3636363636364, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=34.8837209302326, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=35.2380952380952, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 37761; Mature: 37761

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKATILVVDDTPENIDILVGILGGDYRVKVAIDGPKALALASKTTPDLILLDVMMPGMN
CCCEEEEEECCCCCCEEEEEEEECCCEEEEEEECCCCEEEEECCCCCCEEEEEECCCCCC
GYEVCKRLKQEPLTCHIPVIFVTALSDVADETQGFELGAVDYITKPVSAPVVKARVKTHL
HHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHH
ALYDQKCLLEQEVKVRTKELEETRFEIIRRLGRAAEYKDNETGLHVVRMSHYARLLAVQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHC
GLPEYYCELIYNAAPMHDIGKIGTPDSILKKPAKLDATEWVEMQLHAEIGAEIIGEHNDP
CCHHHHHHHHHCCCCCHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
LLEMARRIALTHHEKWDGSGYPNGLSAEEIPIEGRIVAIADVFDALTSIRPYKKAWTVEA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHEEHHH
TMTLIESEAGKQFDPKLVQHFKQIVDELTKVRDAHNETG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDKATILVVDDTPENIDILVGILGGDYRVKVAIDGPKALALASKTTPDLILLDVMMPGMN
CCCEEEEEECCCCCCEEEEEEEECCCEEEEEEECCCCEEEEECCCCCCEEEEEECCCCCC
GYEVCKRLKQEPLTCHIPVIFVTALSDVADETQGFELGAVDYITKPVSAPVVKARVKTHL
HHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHH
ALYDQKCLLEQEVKVRTKELEETRFEIIRRLGRAAEYKDNETGLHVVRMSHYARLLAVQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHC
GLPEYYCELIYNAAPMHDIGKIGTPDSILKKPAKLDATEWVEMQLHAEIGAEIIGEHNDP
CCHHHHHHHHHCCCCCHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
LLEMARRIALTHHEKWDGSGYPNGLSAEEIPIEGRIVAIADVFDALTSIRPYKKAWTVEA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHEEHHH
TMTLIESEAGKQFDPKLVQHFKQIVDELTKVRDAHNETG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]