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The map label for this gene is napA

Identifier: 167622655

GI number: 167622655

Start: 883268

End: 885748

Strand: Reverse

Name: napA

Synonym: Shal_0715

Alternate gene names: 167622655

Gene position: 885748-883268 (Counterclockwise)

Preceding gene: 167622656

Following gene: 167622654

Centisome position: 16.95

GC content: 48.73

Gene sequence:

>2481_bases
ATGAGCATTAGCCGTCGCGAGTTTCTAAAAGCCAACGCCGCCGTTGCAGCAGCAACAGCTGTAGGCGCGACTCTGCCAGT
TAAGATAGTCGAAGCTGCAGAGCAAAAAGATAACATCAAGTGGGACAAAGCACCTTGCCGTTTTTGTGGCGTAGGTTGTA
GCGTTCTCGTGGGCACAGATAATGGCAAAGTTGTCGCCACCAAAGGTGATCCAGAAAGCCCTGTGAACAAAGGCCTTAAC
TGTATTAAAGGCTACTTCCTTTCTAAGATCATGTACGGTAAGGATCGTCTAACTACGCCGCTTTTGCGTATGAAAGATGG
CCAATATGATAAAGAAGGTGAATTCACTCCAATCAGCTGGGATAGCGCATTTGATATCATGGCTGACAAGTGGAAAGACA
CCCTTAAGACTAAAGGTCCAACTGCAGTTGGTATGTTTGGTTCTGGCCAGTGGACTGTTTGGGAAGGCTACGCCGCCTCT
AAACTACACAAGGCGGGTTTCTTAACCAATAACATCGATCCTAACGCACGTCACTGTATGGCATCAGCGGTAGGTGGCTT
TATGCGTACCTTCGGTATCGATGAGCCTATGGGTTGTTACGATGACTTAGAAGCCGCCGATCAGTTCGTGCTTTGGGGCG
CCAACATGGCTGAGATGCACCCAATCCTTTGGGCTCGTCTATCAGATCGCCGCCTAAGCAGCCCAACGAGTCGCGTACAC
GTATTATCAACTTACGAAAACCGTAGCTTCGACTTAGCCGACAACGCCATGGTCTTCCGTCCGCAGTCTGACTTAGTGAT
CCTTAACTATGTTGCCAACTACATCATTCAAAACGATGCAGTAAACAAAGACTTCGTTAACAAGCACACTAAGTTTGCAC
TCGGTACTACCGATATCGGTTACGGTCTACGTCCAGATCATCCACTAGAGAAAAAAGCTAAGAACCCTGGCAACGGTAAG
TCTAAGCCTATCAGCTTCGACGAGTACGCCAAGTTCGTTAGCTCGTACACCCTAGAATATGCAGCCGAGATGAGTGGTGT
TGAGCCAGAAAAGCTAGAGCTTATGGCTAAAGCTTATGCCGATCCAAACGTGAAGATGATGAGCCTGTGGACCATGGGTA
TCAACCAGCACACTCGTGGTGTTTGGGCCAACAACATGCTTTACAACATTCACTTGTTAACCGGTAAGATTGCCACTCCT
GGCAACAGTCCGTTCTCACTAACGGGTCAGCCATCGGCTTGTGGTACAGCACGTGAAGTCGGAACGTTCGCGCACCGTCT
ACCTGCTGACATGGTAGTAGCGAATCCTAAGCACCGTGAGATCACCGAAAAGTTATGGCAAGTTCCAGCGGGCACGATTC
CGCCAAAGCCGGGTTTCCACGCAGTATTGCAAAGCCGTATGCTTAAAGATGGCAAGCTGAACTGTTACTGGACTATGTGT
ACCAATAACATGCAGGCCGGTCCAAACATCAACGATGAAATGTATCCAGGTTTCCGTAACCCAGAAAACTTCATCGTGGT
ATCGGATCCATACCCAACAGTAACAGCTATGGCGGCTGACTTGATCCTACCAACGGCGATGTGGGTAGAAAAAGAAGGGG
CCTACGGTAACGCTGAGCGTCGTACTCACATGTGGCATCAGCAAGTTAAAGCACCAGAAGGTGCTAAATCTGACTTGTGG
CAGCTAATGGAGTTCTCTAAGCGCTTTAAGGTCTCTGAAGTATGGCCTGCAGAGCTTATTGCTAAGCAACCAGAACTTGC
AGATAAGACACTATTTGACGTGCTTTATGCTAACGGCGTGGTCGACAAGTTCCCATCGTCTGAGTGTAAGGGTCAGTACA
ACGACGAAGCTGATGCATTTGGTTTCTATGTCCAAAAGGGTCTATTTGAAGAATACGCTCAGTTTGGTCGCGGCCATGCC
CACGACTTAGCGGACTTTGATACTTACCATGAAACCCGTGGTCTACGCTGGCCAGTGGTTGATGGTAAAGAAACCTTACG
TCGCTTCTCAAAAGGTGACCCTTATGTGAAAGGCGATAAAGAGTTTGATTTCTACGGTAAGCCAGACGGTAAAGCGGTGA
TCTTCGCGCTACCATTTGAGCCCGCAGCAGAAGAGCCAAACGAAGAATACGATATCTGGTTATCAACTGGCCGTGTGCTT
GAGCATTGGCATACAGGTTCGATGACGGCTCGCGTTCCTGAGCTATATCGCGCCTACCCAGATGCACAGATCTTTATGCA
CCCAGAAGATGCTAAAGCTCGTGGTCTTAAGCGTGGCGATGAAGTGATCGTTGCCTCACCTCGCGGTGAAGTGAAGACCC
GTGTAGAAACTAAAGGCCGCAATAAGCCGCCAAGAGGCGTGGCATTTATGCCATTCTTCGATGCGCGCCAGCTGGTCAAC
AAGTTAATACTCGATGCGACAGATCCGCTGTCAAAAGAGACAGACTTCAAGAAGTGTCCAGTTAAAGTGATGAAAGCATA
A

Upstream 100 bases:

>100_bases
CTCGATAACGTTGAAGCGATAAACGCCCTAGAGGGCGTGTTAAATAGCAGTCTAGTTTATCATCAAGTCGATCCAGTAGA
AGAAAAGAGTGAGGAACACC

Downstream 100 bases:

>100_bases
GCTTTTCACTAACAAACTTAACTTTAATCGCTTAAGCAAAAGCGGAGAAAACTAACGCAAATGAGTACGGTCAATCAAAG
TAAGTTCGCAGCCAAAGGCG

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 826; Mature: 825

Protein sequence:

>826_residues
MSISRREFLKANAAVAAATAVGATLPVKIVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTDNGKVVATKGDPESPVNKGLN
CIKGYFLSKIMYGKDRLTTPLLRMKDGQYDKEGEFTPISWDSAFDIMADKWKDTLKTKGPTAVGMFGSGQWTVWEGYAAS
KLHKAGFLTNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTSRVH
VLSTYENRSFDLADNAMVFRPQSDLVILNYVANYIIQNDAVNKDFVNKHTKFALGTTDIGYGLRPDHPLEKKAKNPGNGK
SKPISFDEYAKFVSSYTLEYAAEMSGVEPEKLELMAKAYADPNVKMMSLWTMGINQHTRGVWANNMLYNIHLLTGKIATP
GNSPFSLTGQPSACGTAREVGTFAHRLPADMVVANPKHREITEKLWQVPAGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
TNNMQAGPNINDEMYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGAKSDLW
QLMEFSKRFKVSEVWPAELIAKQPELADKTLFDVLYANGVVDKFPSSECKGQYNDEADAFGFYVQKGLFEEYAQFGRGHA
HDLADFDTYHETRGLRWPVVDGKETLRRFSKGDPYVKGDKEFDFYGKPDGKAVIFALPFEPAAEEPNEEYDIWLSTGRVL
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLKRGDEVIVASPRGEVKTRVETKGRNKPPRGVAFMPFFDARQLVN
KLILDATDPLSKETDFKKCPVKVMKA

Sequences:

>Translated_826_residues
MSISRREFLKANAAVAAATAVGATLPVKIVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTDNGKVVATKGDPESPVNKGLN
CIKGYFLSKIMYGKDRLTTPLLRMKDGQYDKEGEFTPISWDSAFDIMADKWKDTLKTKGPTAVGMFGSGQWTVWEGYAAS
KLHKAGFLTNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTSRVH
VLSTYENRSFDLADNAMVFRPQSDLVILNYVANYIIQNDAVNKDFVNKHTKFALGTTDIGYGLRPDHPLEKKAKNPGNGK
SKPISFDEYAKFVSSYTLEYAAEMSGVEPEKLELMAKAYADPNVKMMSLWTMGINQHTRGVWANNMLYNIHLLTGKIATP
GNSPFSLTGQPSACGTAREVGTFAHRLPADMVVANPKHREITEKLWQVPAGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
TNNMQAGPNINDEMYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGAKSDLW
QLMEFSKRFKVSEVWPAELIAKQPELADKTLFDVLYANGVVDKFPSSECKGQYNDEADAFGFYVQKGLFEEYAQFGRGHA
HDLADFDTYHETRGLRWPVVDGKETLRRFSKGDPYVKGDKEFDFYGKPDGKAVIFALPFEPAAEEPNEEYDIWLSTGRVL
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLKRGDEVIVASPRGEVKTRVETKGRNKPPRGVAFMPFFDARQLVN
KLILDATDPLSKETDFKKCPVKVMKA
>Mature_825_residues
SISRREFLKANAAVAAATAVGATLPVKIVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTDNGKVVATKGDPESPVNKGLNC
IKGYFLSKIMYGKDRLTTPLLRMKDGQYDKEGEFTPISWDSAFDIMADKWKDTLKTKGPTAVGMFGSGQWTVWEGYAASK
LHKAGFLTNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTSRVHV
LSTYENRSFDLADNAMVFRPQSDLVILNYVANYIIQNDAVNKDFVNKHTKFALGTTDIGYGLRPDHPLEKKAKNPGNGKS
KPISFDEYAKFVSSYTLEYAAEMSGVEPEKLELMAKAYADPNVKMMSLWTMGINQHTRGVWANNMLYNIHLLTGKIATPG
NSPFSLTGQPSACGTAREVGTFAHRLPADMVVANPKHREITEKLWQVPAGTIPPKPGFHAVLQSRMLKDGKLNCYWTMCT
NNMQAGPNINDEMYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGAKSDLWQ
LMEFSKRFKVSEVWPAELIAKQPELADKTLFDVLYANGVVDKFPSSECKGQYNDEADAFGFYVQKGLFEEYAQFGRGHAH
DLADFDTYHETRGLRWPVVDGKETLRRFSKGDPYVKGDKEFDFYGKPDGKAVIFALPFEPAAEEPNEEYDIWLSTGRVLE
HWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLKRGDEVIVASPRGEVKTRVETKGRNKPPRGVAFMPFFDARQLVNK
LILDATDPLSKETDFKKCPVKVMKA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily

Homologues:

Organism=Escherichia coli, GI1788534, Length=828, Percent_Identity=72.2222222222222, Blast_Score=1268, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=787, Percent_Identity=24.5235069885642, Blast_Score=205, Evalue=9e-54,
Organism=Escherichia coli, GI3868720, Length=606, Percent_Identity=23.1023102310231, Blast_Score=113, Evalue=6e-26,
Organism=Escherichia coli, GI3868719, Length=493, Percent_Identity=24.1379310344828, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI87081797, Length=829, Percent_Identity=21.351025331725, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPA_SHEHH (B0TSW5)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672949.1
- ProteinModelPortal:   B0TSW5
- SMR:   B0TSW5
- GeneID:   5906601
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0715
- HOGENOM:   HBG715033
- OMA:   NMLYNIH
- ProtClustDB:   PRK13532
- BioCyc:   SHAL458817:SHAL_0715-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01630
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311
- Gene3D:   G3DSA:2.40.40.20
- SMART:   SM00926
- TIGRFAMs:   TIGR01706
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; SSF50692 Asp_decarb_fold

EC number: =1.7.99.4

Molecular weight: Translated: 92340; Mature: 92209

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISRREFLKANAAVAAATAVGATLPVKIVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTD
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEECC
NGKVVATKGDPESPVNKGLNCIKGYFLSKIMYGKDRLTTPLLRMKDGQYDKEGEFTPISW
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCCCCCCCCCCC
DSAFDIMADKWKDTLKTKGPTAVGMFGSGQWTVWEGYAASKLHKAGFLTNNIDPNARHCM
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCHHHHHHHCCCEECCCCCCHHHHH
ASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTSRVH
HHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEE
VLSTYENRSFDLADNAMVFRPQSDLVILNYVANYIIQNDAVNKDFVNKHTKFALGTTDIG
EEEECCCCCEECCCCEEEECCCCCEEEHHHHHHHHHCCCCCCHHHHHCCCEEEECCCCCC
YGLRPDHPLEKKAKNPGNGKSKPISFDEYAKFVSSYTLEYAAEMSGVEPEKLELMAKAYA
CCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPNVKMMSLWTMGINQHTRGVWANNMLYNIHLLTGKIATPGNSPFSLTGQPSACGTAREV
CCCEEEEEEEEECCCCCCCCEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHHHH
GTFAHRLPADMVVANPKHREITEKLWQVPAGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
HHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
TNNMQAGPNINDEMYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAER
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHH
RTHMWHQQVKAPEGAKSDLWQLMEFSKRFKVSEVWPAELIAKQPELADKTLFDVLYANGV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCC
VDKFPSSECKGQYNDEADAFGFYVQKGLFEEYAQFGRGHAHDLADFDTYHETRGLRWPVV
HHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
DGKETLRRFSKGDPYVKGDKEFDFYGKPDGKAVIFALPFEPAAEEPNEEYDIWLSTGRVL
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCHHH
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLKRGDEVIVASPRGEVKTRVETKGR
HHHCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHCCCCCCEEEEECCCCCCEEEHHHCCC
NKPPRGVAFMPFFDARQLVNKLILDATDPLSKETDFKKCPVKVMKA
CCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
SISRREFLKANAAVAAATAVGATLPVKIVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTD
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEECC
NGKVVATKGDPESPVNKGLNCIKGYFLSKIMYGKDRLTTPLLRMKDGQYDKEGEFTPISW
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCCCCCCCCCCC
DSAFDIMADKWKDTLKTKGPTAVGMFGSGQWTVWEGYAASKLHKAGFLTNNIDPNARHCM
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCHHHHHHHCCCEECCCCCCHHHHH
ASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTSRVH
HHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEE
VLSTYENRSFDLADNAMVFRPQSDLVILNYVANYIIQNDAVNKDFVNKHTKFALGTTDIG
EEEECCCCCEECCCCEEEECCCCCEEEHHHHHHHHHCCCCCCHHHHHCCCEEEECCCCCC
YGLRPDHPLEKKAKNPGNGKSKPISFDEYAKFVSSYTLEYAAEMSGVEPEKLELMAKAYA
CCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPNVKMMSLWTMGINQHTRGVWANNMLYNIHLLTGKIATPGNSPFSLTGQPSACGTAREV
CCCEEEEEEEEECCCCCCCCEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHHHH
GTFAHRLPADMVVANPKHREITEKLWQVPAGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
HHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
TNNMQAGPNINDEMYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAER
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHH
RTHMWHQQVKAPEGAKSDLWQLMEFSKRFKVSEVWPAELIAKQPELADKTLFDVLYANGV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCC
VDKFPSSECKGQYNDEADAFGFYVQKGLFEEYAQFGRGHAHDLADFDTYHETRGLRWPVV
HHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
DGKETLRRFSKGDPYVKGDKEFDFYGKPDGKAVIFALPFEPAAEEPNEEYDIWLSTGRVL
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCHHH
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLKRGDEVIVASPRGEVKTRVETKGR
HHHCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHCCCCCCEEEEECCCCCCEEEHHHCCC
NKPPRGVAFMPFFDARQLVNKLILDATDPLSKETDFKKCPVKVMKA
CCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA