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The map label for this gene is rsmE [H]

Identifier: 167622641

GI number: 167622641

Start: 864198

End: 864929

Strand: Reverse

Name: rsmE [H]

Synonym: Shal_0701

Alternate gene names: 167622641

Gene position: 864929-864198 (Counterclockwise)

Preceding gene: 167622642

Following gene: 167622640

Centisome position: 16.55

GC content: 49.32

Gene sequence:

>732_bases
ATGAGAGTGCCAAGAATTTATCAAGAATCATCGCTAGTCATCGGCCAGGCAGTCGCTTTAGATGATGAGGCTGCGGGTCA
CGTTGGTCGCGTCCTAAGAATGTCCGTTGGCGAGCAAGTTAGCCTTTTTAATGGTGACGGCAATGACTACCTAGCCGAAA
TCATCAGCGCCAGTAAGAAAAATGTCGAAGTCAAGGTCCTCTCTTGCGAGGCCAATAACAGCGAGTCACCACTGCACTTA
CATTTAGGTCAGGTGATTTCACGCGGCGATAGAATGGATTTTACCATTCAAAAGTCTGTAGAACTTGGTGTAAATACGAT
TACACCACTGTTTTCAGAGCGCTGCGGCGTTAAGCTTAGCGGTGAACGCTTAGAGAAGAAAATTCAGCAGTGGCAGAAGA
TTGTGGTCAGTGCTTGTGAGCAATCGGGTCGCAGTGTCGTTCCTGTAATTCGCCCAGCTATGCAGCTTAGCGATTGGTGC
GCCGAGCCGTCCGATGCCGTCAAACTCAATCTACATCCAAGAGCCTCTCACGGCGTGAATGGCCTAACCTTACCGAATAA
CAGAGTAAGACTCGTTATCGGCCCAGAAGGTGGGTTATCCGCACCCGAAATCGCCATGACCGAAGAGCACCAATTTACCG
ATGTATTGTTAGGACCACGCGTACTGCGCACCGAAACTGCGGCACTAACAACAATCAGTGCTCTACAGCTCAAGTTCGGG
GATATTGGATAA

Upstream 100 bases:

>100_bases
GAGATTGCCAAAGTTCAAGGCAATCACAATGAGTTTGTACAAGCTCAATGTCAGCGCTTAAAAGACTCGCAAACATACGC
AGAATAAGGCTAGTAAAATC

Downstream 100 bases:

>100_bases
GCCCACGGGTATTGCTACACCTCAAAGCGGTGGCCTGTATCACGCACCGCTACATAGAAACGTTTATGAGTACAACGCTA
GAAAATAGGCTCACTCGCCC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRVPRIYQESSLVIGQAVALDDEAAGHVGRVLRMSVGEQVSLFNGDGNDYLAEIISASKKNVEVKVLSCEANNSESPLHL
HLGQVISRGDRMDFTIQKSVELGVNTITPLFSERCGVKLSGERLEKKIQQWQKIVVSACEQSGRSVVPVIRPAMQLSDWC
AEPSDAVKLNLHPRASHGVNGLTLPNNRVRLVIGPEGGLSAPEIAMTEEHQFTDVLLGPRVLRTETAALTTISALQLKFG
DIG

Sequences:

>Translated_243_residues
MRVPRIYQESSLVIGQAVALDDEAAGHVGRVLRMSVGEQVSLFNGDGNDYLAEIISASKKNVEVKVLSCEANNSESPLHL
HLGQVISRGDRMDFTIQKSVELGVNTITPLFSERCGVKLSGERLEKKIQQWQKIVVSACEQSGRSVVPVIRPAMQLSDWC
AEPSDAVKLNLHPRASHGVNGLTLPNNRVRLVIGPEGGLSAPEIAMTEEHQFTDVLLGPRVLRTETAALTTISALQLKFG
DIG
>Mature_243_residues
MRVPRIYQESSLVIGQAVALDDEAAGHVGRVLRMSVGEQVSLFNGDGNDYLAEIISASKKNVEVKVLSCEANNSESPLHL
HLGQVISRGDRMDFTIQKSVELGVNTITPLFSERCGVKLSGERLEKKIQQWQKIVVSACEQSGRSVVPVIRPAMQLSDWC
AEPSDAVKLNLHPRASHGVNGLTLPNNRVRLVIGPEGGLSAPEIAMTEEHQFTDVLLGPRVLRTETAALTTISALQLKFG
DIG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=65.4320987654321, Blast_Score=334, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26385; Mature: 26385

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVPRIYQESSLVIGQAVALDDEAAGHVGRVLRMSVGEQVSLFNGDGNDYLAEIISASKK
CCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCC
NVEVKVLSCEANNSESPLHLHLGQVISRGDRMDFTIQKSVELGVNTITPLFSERCGVKLS
CEEEEEEEECCCCCCCCEEEEHHHHHCCCCCCEEEEECHHHCCCHHHHHHHHHHCCCEEC
GERLEKKIQQWQKIVVSACEQSGRSVVPVIRPAMQLSDWCAEPSDAVKLNLHPRASHGVN
HHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
GLTLPNNRVRLVIGPEGGLSAPEIAMTEEHQFTDVLLGPRVLRTETAALTTISALQLKFG
CEECCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHEEECC
DIG
CCC
>Mature Secondary Structure
MRVPRIYQESSLVIGQAVALDDEAAGHVGRVLRMSVGEQVSLFNGDGNDYLAEIISASKK
CCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCC
NVEVKVLSCEANNSESPLHLHLGQVISRGDRMDFTIQKSVELGVNTITPLFSERCGVKLS
CEEEEEEEECCCCCCCCEEEEHHHHHCCCCCCEEEEECHHHCCCHHHHHHHHHHCCCEEC
GERLEKKIQQWQKIVVSACEQSGRSVVPVIRPAMQLSDWCAEPSDAVKLNLHPRASHGVN
HHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
GLTLPNNRVRLVIGPEGGLSAPEIAMTEEHQFTDVLLGPRVLRTETAALTTISALQLKFG
CEECCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHEEECC
DIG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]