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The map label for this gene is pyrB

Identifier: 167622594

GI number: 167622594

Start: 799854

End: 800786

Strand: Direct

Name: pyrB

Synonym: Shal_0654

Alternate gene names: 167622594

Gene position: 799854-800786 (Clockwise)

Preceding gene: 167622593

Following gene: 167622595

Centisome position: 15.3

GC content: 44.16

Gene sequence:

>933_bases
ATGAGTAACCCCCTATACAACAAAAATATAATCTCAATTACCGACTTGTCTCGTGCTGAGCTTGAGTTGATAGTCTCTAC
CGCAAATGAACTGAAGCAACACCCTCGCCCTGATTTATTAAAAAATAAAGTCATCGCCAGCTGTTTTTTTGAAGCCTCGA
CCCGCACTCGCCTCTCTTTCGAGACCGCGGTGCAAAGGTTAGGTGGTAGCGTTATTGGCTTCCCTGACAGTGGCAATACC
TCTTTGGGCAAGAAAGGTGAAACCTTAGCGGATTCAGTGCAGGTGATCTCCTCCTATAGTGATGCATTTTTTATGCGTCA
TAATCAAGAAGGCGCAGCGCGACTTGCTAGTGAATTTTCATCGGTTCCCGTGATTAATGGTGGCGATGGCTCTAATCAAC
ATCCGACTCAGACCTTGCTCGACTTGTTTAGTATCTATGAGACCCAAGGGACCTTAGATAAGTTACAGGTGGCGTTTGTT
GGCGACCTAAAATATGGACGCACCGTCCATTCATTGACCCAAGCCTTATCTTTGTTTGATTGTGAGTTCCACTTTGTTGC
GCCTCCAGCACTCTCTATGCCCGAATATATTATCGATGAGCTTAAGGAAAAAGGCTGCACATTCACTCAGTATGACAGCT
TAGATGGCGTACTCTCGAAACTGGATATTCTGTATATGACTCGAGTGCAGAAAGAGCGCTTCGACGAAACTGAGTATCAG
CATATGAAATCCAGTTTTATCCTAACTGCTCAGATGTTTGAAGGTGTAAAGGATAATCTTAAAGTATTACATCCCCTCCC
TAGAGTTGATGAGATCACCACCGATGTAGATAGCACTCCATATGCTTACTATTTCCAACAGGCTAAAAATGGCGTCTATG
CTCGTCAGGCTTTGCTTGCCTTGGTATTGACCAACGAATTTGGAGATAAGTAA

Upstream 100 bases:

>100_bases
TAGGTATATACCTCGAACTAGCGGGATATATCTATACTCATTGATGGTAAACGAGTACGATTTTACATCGATTTTGCCAC
CGGAAAAGGACGCTGTAACA

Downstream 100 bases:

>100_bases
TGAAAAAGCATCTAAAAGTTGAAGCTATTGAGCAAGGTACAGTTATCGATCATATTGCTCCTGGTCAAGGTATCAAGATT
TTAAAATTCTTTGAACTTTC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase 1; ATCase 1

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSNPLYNKNIISITDLSRAELELIVSTANELKQHPRPDLLKNKVIASCFFEASTRTRLSFETAVQRLGGSVIGFPDSGNT
SLGKKGETLADSVQVISSYSDAFFMRHNQEGAARLASEFSSVPVINGGDGSNQHPTQTLLDLFSIYETQGTLDKLQVAFV
GDLKYGRTVHSLTQALSLFDCEFHFVAPPALSMPEYIIDELKEKGCTFTQYDSLDGVLSKLDILYMTRVQKERFDETEYQ
HMKSSFILTAQMFEGVKDNLKVLHPLPRVDEITTDVDSTPYAYYFQQAKNGVYARQALLALVLTNEFGDK

Sequences:

>Translated_310_residues
MSNPLYNKNIISITDLSRAELELIVSTANELKQHPRPDLLKNKVIASCFFEASTRTRLSFETAVQRLGGSVIGFPDSGNT
SLGKKGETLADSVQVISSYSDAFFMRHNQEGAARLASEFSSVPVINGGDGSNQHPTQTLLDLFSIYETQGTLDKLQVAFV
GDLKYGRTVHSLTQALSLFDCEFHFVAPPALSMPEYIIDELKEKGCTFTQYDSLDGVLSKLDILYMTRVQKERFDETEYQ
HMKSSFILTAQMFEGVKDNLKVLHPLPRVDEITTDVDSTPYAYYFQQAKNGVYARQALLALVLTNEFGDK
>Mature_309_residues
SNPLYNKNIISITDLSRAELELIVSTANELKQHPRPDLLKNKVIASCFFEASTRTRLSFETAVQRLGGSVIGFPDSGNTS
LGKKGETLADSVQVISSYSDAFFMRHNQEGAARLASEFSSVPVINGGDGSNQHPTQTLLDLFSIYETQGTLDKLQVAFVG
DLKYGRTVHSLTQALSLFDCEFHFVAPPALSMPEYIIDELKEKGCTFTQYDSLDGVLSKLDILYMTRVQKERFDETEYQH
MKSSFILTAQMFEGVKDNLKVLHPLPRVDEITTDVDSTPYAYYFQQAKNGVYARQALLALVLTNEFGDK

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=45.3947368421053, Blast_Score=268, Evalue=6e-72,
Organism=Homo sapiens, GI38788445, Length=321, Percent_Identity=25.5451713395639, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=67.9611650485437, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1786469, Length=335, Percent_Identity=26.2686567164179, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790703, Length=343, Percent_Identity=26.8221574344023, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=42.1052631578947, Blast_Score=237, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=42.2077922077922, Blast_Score=257, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6322373, Length=331, Percent_Identity=27.7945619335347, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=43.9102564102564, Blast_Score=252, Evalue=2e-67,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB1_SHEHH (B0TSQ4)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672888.1
- ProteinModelPortal:   B0TSQ4
- SMR:   B0TSQ4
- GeneID:   5906714
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0654
- HOGENOM:   HBG579429
- OMA:   FKQSFYG
- ProtClustDB:   PRK00856
- BioCyc:   SHAL458817:SHAL_0654-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34627; Mature: 34495

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPLYNKNIISITDLSRAELELIVSTANELKQHPRPDLLKNKVIASCFFEASTRTRLSF
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHH
ETAVQRLGGSVIGFPDSGNTSLGKKGETLADSVQVISSYSDAFFMRHNQEGAARLASEFS
HHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHH
SVPVINGGDGSNQHPTQTLLDLFSIYETQGTLDKLQVAFVGDLKYGRTVHSLTQALSLFD
CCCEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCHHHHHHHHHHHHHC
CEFHFVAPPALSMPEYIIDELKEKGCTFTQYDSLDGVLSKLDILYMTRVQKERFDETEYQ
CCEEEECCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
HMKSSFILTAQMFEGVKDNLKVLHPLPRVDEITTDVDSTPYAYYFQQAKNGVYARQALLA
HHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHH
LVLTNEFGDK
HHHHHHCCCC
>Mature Secondary Structure 
SNPLYNKNIISITDLSRAELELIVSTANELKQHPRPDLLKNKVIASCFFEASTRTRLSF
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHH
ETAVQRLGGSVIGFPDSGNTSLGKKGETLADSVQVISSYSDAFFMRHNQEGAARLASEFS
HHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHH
SVPVINGGDGSNQHPTQTLLDLFSIYETQGTLDKLQVAFVGDLKYGRTVHSLTQALSLFD
CCCEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCHHHHHHHHHHHHHC
CEFHFVAPPALSMPEYIIDELKEKGCTFTQYDSLDGVLSKLDILYMTRVQKERFDETEYQ
CCEEEECCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
HMKSSFILTAQMFEGVKDNLKVLHPLPRVDEITTDVDSTPYAYYFQQAKNGVYARQALLA
HHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHH
LVLTNEFGDK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA