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The map label for this gene is mepA [H]

Identifier: 167622544

GI number: 167622544

Start: 729845

End: 730741

Strand: Direct

Name: mepA [H]

Synonym: Shal_0604

Alternate gene names: 167622544

Gene position: 729845-730741 (Clockwise)

Preceding gene: 167622543

Following gene: 167622546

Centisome position: 13.96

GC content: 50.06

Gene sequence:

>897_bases
ATGATCTTAAACGATGATTCTGAGGTGATTATGTTGGCGAGTAAGTTGTGGGGAATTTTACGGCGGACGCTGGCGATTGT
CGGTTTAAATCTACTCGTTTCCTCGGCTGCGATAGCTAATCTATGGGAGGCCGCTGATACTCCCTATGTTAGCCAAGCTA
GCGCTATCGGCAGTTATGCTAATGGCTGCCTAAAAGGGGCTGAGGCTCTACCGTTAAACGGTAACGGCTATCAGGTTATC
CGTCCTCAACGGCAGCGATACTATGGCCACCCTGAGCTTATCGAATTCATCGAGCAGTATTCCACGAGTCTGAATCGCCT
TGGCAGTGACGATATTTTGATTGCTGATATGTCGATGCCAAGAGGGGGTAATTTTACCCAAGGCCACACGAGCCATCAGA
TAGGACTCGATGTTGATATCTGGCTTAAACTTACGGCGGAGCCATTATTAGACAGTGAGCGAGAGCAGCCCACACCGCTC
AAGCTGGTTAACCAAAAGCAGTTTAAACTCGATACTCAGTTGTGGTCGAATACCCAGACCCAGATGGTCAAGCTAGCTGC
ACAAGATGCGCGCGTTGCACGAATTTTTGTTAGCCCAGTGATAAAGCAGCACTTGTGTGATTTAGGCTTAGATGATGACG
CTTGGCTTGAGAAGGTGCGTCCTTGGTGGGGGCACACTTACCATATGCATGTGAGACTGCGCTGCCCAAAGGGGGATGAG
TTCTGCCGTGAGCAGGCGAAAATCCCTAAGGGTAACGGTTGTAGCGAGCTAAGCTGGTGGAGGCAACGGTTGACGCAGGC
CAAGGTGGCAAAAGTCGAAAAACCAAAAGTTAAGCCGAAGCAGGTAAAAGCCAAGCGAAAGCCTAAGCTGTGCGAAGCCG
TTGTCGCAGCTAAGTAG

Upstream 100 bases:

>100_bases
TCTCTCTTCCTCTCTTTCTCTCTCTTCCTAACTTCTCGGCTAAACCCAGTGCCTTGTCTTGGGGAAATTCATCAGCGTAA
ATCTCAGCTATCTTTTATAG

Downstream 100 bases:

>100_bases
TGGTAGTGCCAAGTTGTTGTTAATGTTAGGGAAGATGCGGATGTGAGGGTTTGGAGTTAGCTCTATTGAGAGTACAAAGC
GCCCCGTGTAGGTGCGCTTT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MILNDDSEVIMLASKLWGILRRTLAIVGLNLLVSSAAIANLWEAADTPYVSQASAIGSYANGCLKGAEALPLNGNGYQVI
RPQRQRYYGHPELIEFIEQYSTSLNRLGSDDILIADMSMPRGGNFTQGHTSHQIGLDVDIWLKLTAEPLLDSEREQPTPL
KLVNQKQFKLDTQLWSNTQTQMVKLAAQDARVARIFVSPVIKQHLCDLGLDDDAWLEKVRPWWGHTYHMHVRLRCPKGDE
FCREQAKIPKGNGCSELSWWRQRLTQAKVAKVEKPKVKPKQVKAKRKPKLCEAVVAAK

Sequences:

>Translated_298_residues
MILNDDSEVIMLASKLWGILRRTLAIVGLNLLVSSAAIANLWEAADTPYVSQASAIGSYANGCLKGAEALPLNGNGYQVI
RPQRQRYYGHPELIEFIEQYSTSLNRLGSDDILIADMSMPRGGNFTQGHTSHQIGLDVDIWLKLTAEPLLDSEREQPTPL
KLVNQKQFKLDTQLWSNTQTQMVKLAAQDARVARIFVSPVIKQHLCDLGLDDDAWLEKVRPWWGHTYHMHVRLRCPKGDE
FCREQAKIPKGNGCSELSWWRQRLTQAKVAKVEKPKVKPKQVKAKRKPKLCEAVVAAK
>Mature_298_residues
MILNDDSEVIMLASKLWGILRRTLAIVGLNLLVSSAAIANLWEAADTPYVSQASAIGSYANGCLKGAEALPLNGNGYQVI
RPQRQRYYGHPELIEFIEQYSTSLNRLGSDDILIADMSMPRGGNFTQGHTSHQIGLDVDIWLKLTAEPLLDSEREQPTPL
KLVNQKQFKLDTQLWSNTQTQMVKLAAQDARVARIFVSPVIKQHLCDLGLDDDAWLEKVRPWWGHTYHMHVRLRCPKGDE
FCREQAKIPKGNGCSELSWWRQRLTQAKVAKVEKPKVKPKQVKAKRKPKLCEAVVAAK

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=259, Percent_Identity=42.8571428571429, Blast_Score=210, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 33576; Mature: 33576

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNDDSEVIMLASKLWGILRRTLAIVGLNLLVSSAAIANLWEAADTPYVSQASAIGSYA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
NGCLKGAEALPLNGNGYQVIRPQRQRYYGHPELIEFIEQYSTSLNRLGSDDILIADMSMP
HHHHCCCCCCCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
RGGNFTQGHTSHQIGLDVDIWLKLTAEPLLDSEREQPTPLKLVNQKQFKLDTQLWSNTQT
CCCCCCCCCCCCCCCCCEEEEEEEECCHHCCCCCCCCCCHHHCCCHHHHHHHHHHCCHHH
QMVKLAAQDARVARIFVSPVIKQHLCDLGLDDDAWLEKVRPWWGHTYHMHVRLRCPKGDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHCCEEEEEEEEECCCCHH
FCREQAKIPKGNGCSELSWWRQRLTQAKVAKVEKPKVKPKQVKAKRKPKLCEAVVAAK
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MILNDDSEVIMLASKLWGILRRTLAIVGLNLLVSSAAIANLWEAADTPYVSQASAIGSYA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
NGCLKGAEALPLNGNGYQVIRPQRQRYYGHPELIEFIEQYSTSLNRLGSDDILIADMSMP
HHHHCCCCCCCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
RGGNFTQGHTSHQIGLDVDIWLKLTAEPLLDSEREQPTPLKLVNQKQFKLDTQLWSNTQT
CCCCCCCCCCCCCCCCCEEEEEEEECCHHCCCCCCCCCCHHHCCCHHHHHHHHHHCCHHH
QMVKLAAQDARVARIFVSPVIKQHLCDLGLDDDAWLEKVRPWWGHTYHMHVRLRCPKGDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHCCEEEEEEEEECCCCHH
FCREQAKIPKGNGCSELSWWRQRLTQAKVAKVEKPKVKPKQVKAKRKPKLCEAVVAAK
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]