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The map label for this gene is purD [H]

Identifier: 167622427

GI number: 167622427

Start: 585995

End: 587296

Strand: Reverse

Name: purD [H]

Synonym: Shal_0487

Alternate gene names: 167622427

Gene position: 587296-585995 (Counterclockwise)

Preceding gene: 167622428

Following gene: 167622426

Centisome position: 11.24

GC content: 51.84

Gene sequence:

>1302_bases
ATGAGAGTATTGATTATAGGTGGCGGCGGTCGTGAACATGCATTGGCATGGAAAGCGGCGCAATCGACACAAGTTGAAAC
CGTCTTTGTTGCTCCGGGCAACGCTGGTACGGCATTAGAGCCAAAGCTTGAAAACGTAGCGATTAATGTTGAAGAGATCT
CGGCATTGGTGGCGTTTGCGACAGAGCAAAAAGTCGCGATCACCATAGTTGGCCCAGAAGTGCCGTTGGCACTGGGTGTG
GTTGATGCTTTTAACGAAGCTGGCCTACCTATCTTTGGCCCAACCAAAGGCGCCGCGCAGCTAGAATCGTCTAAAGCTTT
CACCAAAGACTTCTTAGCGCGTCACAATATCCCTACTGCGGCTTACTCAAACTTTACCGAGATAGCTCCCGCTAAAGCCT
ATGTGACTCAAGTTACTGGCTTAACGGGTTTCCCTATCGTGATTAAGGCCGATGGCCTAGCGGCAGGTAAAGGCGTGATC
ATCGCAGCAGACCAAGTTGAAGCCGATGCCGCTATCGAAGATATGCTGGCAGGTAACAAGTTCGGTGAAGCGGGTTCTCG
CGTGGTTATCGAGGAGTTCCTCAGAGGTGAAGAAGCCAGCTTTATCGTGATGGTGGATGGTAAGAACATTCTTGCTATGG
CCACCAGCCAAGATCATAAAGCGCGCGATAACGGCGATCATGGTCCCAACACTGGCGGCATGGGCGCATACTCACCAGCC
CCCGTTGTGACCCAAGCGGTTCACGACTGGACTATCGAGCACGTTATTCGCCCAACCGTTGATGGCATGGCAGCTGAAGG
CAACGTCTACACAGGTTTCCTATACGCAGGCCTGATGATTTCACCTGATGGCAGCGCTAAAGTGCTTGAGTACAACTGTC
GCTTTGGTGACCCTGAGACGCAGCCGATTATGATGCGCCTACAATCAGACATCGTTGAGCTTTGCTTAGCCTCGACTCGC
GGCGAGCTTGACCAAGTCACTGCGGAGTTTGATGACCGTGCGGCCGTCGGCGTTGTGTTAGCCGCTGGCGGATATCCTGA
TGCATACCGTAAGCATGATGTAATTGAAGGCCTGACTTTAGGTAACAATCATGCAAAAGTGTTCCATGCGGGAACCTGTA
TGAAAGAGGGACTCGTGGTGACTAATGGCGGCCGCGTATTATGTGCGACAGCACTGGGTAATACAGTGACTGAAGCACAG
CAATCAGCTTACGCTTTAGTGGATGCTGTCCATTGGGATGATGCCTACTTCCGCACCGACATTGCATACCGCGCAATAGC
TCGTGAAAATGGCGAAGGTTAA

Upstream 100 bases:

>100_bases
ATCGGTTATTGAGCAAAGAAAACACACGGAGTGTATGCCAATACATGAGTATGTTTGATGCCGTTCAAGGACCGATGAAG
AAGCGATTAAGGATTAAAAA

Downstream 100 bases:

>100_bases
CTAACTAAAAACTATTGGGATGATGGCTGTTATAACTTAACAGAACCGACATTTTCCGCACCGACATTGCATACCGCGCG
ATTGCTCGTGAAAATGGCGA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MRVLIIGGGGREHALAWKAAQSTQVETVFVAPGNAGTALEPKLENVAINVEEISALVAFATEQKVAITIVGPEVPLALGV
VDAFNEAGLPIFGPTKGAAQLESSKAFTKDFLARHNIPTAAYSNFTEIAPAKAYVTQVTGLTGFPIVIKADGLAAGKGVI
IAADQVEADAAIEDMLAGNKFGEAGSRVVIEEFLRGEEASFIVMVDGKNILAMATSQDHKARDNGDHGPNTGGMGAYSPA
PVVTQAVHDWTIEHVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPETQPIMMRLQSDIVELCLASTR
GELDQVTAEFDDRAAVGVVLAAGGYPDAYRKHDVIEGLTLGNNHAKVFHAGTCMKEGLVVTNGGRVLCATALGNTVTEAQ
QSAYALVDAVHWDDAYFRTDIAYRAIARENGEG

Sequences:

>Translated_433_residues
MRVLIIGGGGREHALAWKAAQSTQVETVFVAPGNAGTALEPKLENVAINVEEISALVAFATEQKVAITIVGPEVPLALGV
VDAFNEAGLPIFGPTKGAAQLESSKAFTKDFLARHNIPTAAYSNFTEIAPAKAYVTQVTGLTGFPIVIKADGLAAGKGVI
IAADQVEADAAIEDMLAGNKFGEAGSRVVIEEFLRGEEASFIVMVDGKNILAMATSQDHKARDNGDHGPNTGGMGAYSPA
PVVTQAVHDWTIEHVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPETQPIMMRLQSDIVELCLASTR
GELDQVTAEFDDRAAVGVVLAAGGYPDAYRKHDVIEGLTLGNNHAKVFHAGTCMKEGLVVTNGGRVLCATALGNTVTEAQ
QSAYALVDAVHWDDAYFRTDIAYRAIARENGEG
>Mature_433_residues
MRVLIIGGGGREHALAWKAAQSTQVETVFVAPGNAGTALEPKLENVAINVEEISALVAFATEQKVAITIVGPEVPLALGV
VDAFNEAGLPIFGPTKGAAQLESSKAFTKDFLARHNIPTAAYSNFTEIAPAKAYVTQVTGLTGFPIVIKADGLAAGKGVI
IAADQVEADAAIEDMLAGNKFGEAGSRVVIEEFLRGEEASFIVMVDGKNILAMATSQDHKARDNGDHGPNTGGMGAYSPA
PVVTQAVHDWTIEHVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPETQPIMMRLQSDIVELCLASTR
GELDQVTAEFDDRAAVGVVLAAGGYPDAYRKHDVIEGLTLGNNHAKVFHAGTCMKEGLVVTNGGRVLCATALGNTVTEAQ
QSAYALVDAVHWDDAYFRTDIAYRAIARENGEG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=432, Percent_Identity=47.6851851851852, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI209869995, Length=432, Percent_Identity=47.6851851851852, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI209869993, Length=432, Percent_Identity=47.6851851851852, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI28416899, Length=429, Percent_Identity=47.5524475524476, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI1790438, Length=432, Percent_Identity=68.5185185185185, Blast_Score=587, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=39.9527186761229, Blast_Score=288, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6321203, Length=451, Percent_Identity=43.0155210643016, Blast_Score=343, Evalue=2e-95,
Organism=Drosophila melanogaster, GI62471619, Length=436, Percent_Identity=44.2660550458716, Blast_Score=338, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24582400, Length=436, Percent_Identity=44.2660550458716, Blast_Score=338, Evalue=6e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45606; Mature: 45606

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLIIGGGGREHALAWKAAQSTQVETVFVAPGNAGTALEPKLENVAINVEEISALVAFA
CEEEEEECCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHEE
TEQKVAITIVGPEVPLALGVVDAFNEAGLPIFGPTKGAAQLESSKAFTKDFLARHNIPTA
CCCEEEEEEECCCCCEEEHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCHH
AYSNFTEIAPAKAYVTQVTGLTGFPIVIKADGLAAGKGVIIAADQVEADAAIEDMLAGNK
HHCCCHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCC
FGEAGSRVVIEEFLRGEEASFIVMVDGKNILAMATSQDHKARDNGDHGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PVVTQAVHDWTIEHVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPET
CHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCC
QPIMMRLQSDIVELCLASTRGELDQVTAEFDDRAAVGVVLAAGGYPDAYRKHDVIEGLTL
CHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHCCEEE
GNNHAKVFHAGTCMKEGLVVTNGGRVLCATALGNTVTEAQQSAYALVDAVHWDDAYFRTD
CCCCEEEEECCCHHHCCEEEECCCCEEEEEHHCCHHHHHHHHHEEEEEEEECCCCHHHHH
IAYRAIARENGEG
HHHHHHHHCCCCC
>Mature Secondary Structure
MRVLIIGGGGREHALAWKAAQSTQVETVFVAPGNAGTALEPKLENVAINVEEISALVAFA
CEEEEEECCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHEE
TEQKVAITIVGPEVPLALGVVDAFNEAGLPIFGPTKGAAQLESSKAFTKDFLARHNIPTA
CCCEEEEEEECCCCCEEEHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCHH
AYSNFTEIAPAKAYVTQVTGLTGFPIVIKADGLAAGKGVIIAADQVEADAAIEDMLAGNK
HHCCCHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCC
FGEAGSRVVIEEFLRGEEASFIVMVDGKNILAMATSQDHKARDNGDHGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PVVTQAVHDWTIEHVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPET
CHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCC
QPIMMRLQSDIVELCLASTRGELDQVTAEFDDRAAVGVVLAAGGYPDAYRKHDVIEGLTL
CHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHCCEEE
GNNHAKVFHAGTCMKEGLVVTNGGRVLCATALGNTVTEAQQSAYALVDAVHWDDAYFRTD
CCCCEEEEECCCHHHCCEEEECCCCEEEEEHHCCHHHHHHHHHEEEEEEEECCCCHHHHH
IAYRAIARENGEG
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]