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The map label for this gene is smpB

Identifier: 16752702

GI number: 16752702

Start: 459214

End: 459711

Strand: Direct

Name: smpB

Synonym: CP0421

Alternate gene names: 16752702

Gene position: 459214-459711 (Clockwise)

Preceding gene: 16752701

Following gene: 16752706

Centisome position: 37.34

GC content: 42.86

Gene sequence:

>498_bases
TTGTTATACTGGTTTTTGTCCCCAATTATGGGGGAGGATCTTATGGCACAAAAAGAAATTGTTTCTAATCGCAAGGCTCT
GCGTAACTATGAAGTTATAGAGACTTTAGAAGCAGGCATCGTTTTGACTGGGACTGAGATTAAGTCGTTGCGCGATCATG
GGGGAAACCTCGGTGATGCTTATGTCATTGTTTCTAAAGGTGAGGGGTGGTTATTAAACGCGAGTATTGCTCCCTATCGG
TTTGGAAATATCTATAACCATGAGGAGCGTCGTAAACGTAAACTCCTTCTTCATAGATATGAACTTCGTAAGTTAGAGGG
TAAGATTGCTCAAAAGGGCATGACTTTGATTCCTCTGGGAATGTTKCTGAGTCGCGGCTATGTTAAGGTACGTTTGGGTT
GTTGTCGTGGGAAAAAAGCTTATGATAAGCGTCGTACGATCATAGAAAGAGAAAAGGAACGTGAAGTTGCCGCTGCTATG
AAGAGGCGCCATCATTGA

Upstream 100 bases:

>100_bases
GATATTTTACTAATAAAAAAGGCTTTAGATTGAGGAAATCTTCCCTGGAAATCAAGGAAAGAGGATTCTAATCATTGTCT
TAAGAAGGAAAAATTGCTTT

Downstream 100 bases:

>100_bases
TATAGGTTAGGATATGGTGTTCTTCAGCCCACTGTTTTGCTTCTATTTTAGAATCAAAAGTCATGAGGACTGTGGCAATA
GCGTCGGCGTATGCGCAGCT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MLYWFLSPIMGEDLMAQKEIVSNRKALRNYEVIETLEAGIVLTGTEIKSLRDHGGNLGDAYVIVSKGEGWLLNASIAPYR
FGNIYNHEERRKRKLLLHRYELRKLEGKIAQKGMTLIPLGMXLSRGYVKVRLGCCRGKKAYDKRRTIIEREKEREVAAAM
KRRHH

Sequences:

>Translated_165_residues
MLYWFLSPIMGEDLMAQKEIVSNRKALRNYEVIETLEAGIVLTGTEIKSLRDHGGNLGDAYVIVSKGEGWLLNASIAPYR
FGNIYNHEERRKRKLLLHRYELRKLEGKIAQKGMTLIPLGMXLSRGYVKVRLGCCRGKKAYDKRRTIIEREKEREVAAAM
KRRHH
>Mature_165_residues
MLYWFLSPIMGEDLMAQKEIVSNRKALRNYEVIETLEAGIVLTGTEIKSLRDHGGNLGDAYVIVSKGEGWLLNASIAPYR
FGNIYNHEERRKRKLLLHRYELRKLEGKIAQKGMTLIPLGMXLSRGYVKVRLGCCRGKKAYDKRRTIIEREKEREVAAAM
KRRHH

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=146, Percent_Identity=37.6712328767123, Blast_Score=100, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 19057; Mature: 19057

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYWFLSPIMGEDLMAQKEIVSNRKALRNYEVIETLEAGIVLTGTEIKSLRDHGGNLGDA
CEEEEECCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCCCCE
YVIVSKGEGWLLNASIAPYRFGNIYNHEERRKRKLLLHRYELRKLEGKIAQKGMTLIPLG
EEEEECCCCEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
MXLSRGYVKVRLGCCRGKKAYDKRRTIIEREKEREVAAAMKRRHH
HHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLYWFLSPIMGEDLMAQKEIVSNRKALRNYEVIETLEAGIVLTGTEIKSLRDHGGNLGDA
CEEEEECCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCCCCE
YVIVSKGEGWLLNASIAPYRFGNIYNHEERRKRKLLLHRYELRKLEGKIAQKGMTLIPLG
EEEEECCCCEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
MXLSRGYVKVRLGCCRGKKAYDKRRTIIEREKEREVAAAMKRRHH
HHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]