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The map label for this gene is ruvB

Identifier: 16752648

GI number: 16752648

Start: 402053

End: 403066

Strand: Direct

Name: ruvB

Synonym: CP0365

Alternate gene names: 16752648

Gene position: 402053-403066 (Clockwise)

Preceding gene: 16752647

Following gene: 16752649

Centisome position: 32.69

GC content: 42.5

Gene sequence:

>1014_bases
ATGACGCATCAAGTAGCTGTCTTGCATCAGGATAAAAAATTTGATGTTTCGTTAAGACCTAAAGGGTTAGAAGAATTTTA
TGGACAGCATCATTTAAAAGAACGCCTAGATCTATTTCTTTGCGCAGCATTGCAACGAGGAGAAGTTCCAGGACATTGCT
TGTTTTTTGGACCCCCAGGCTTAGGGAAAACCTCACTTGCTCACATCGTTGCCTACACCGTGGGGAAAGGGCTGGTCTTG
GCATCAGGGCCTCAGTTAATCAAACCCTCGGACCTGTTAGGACTTTTAACTAGTTTGCAAGAAGGGGACGTGTTTTTCAT
CGATGAGATCCATCGTATGGGGAAAGTTGCTGAGGAATACCTGTATTCTGCAATGGAAGATTTCAAAGTCGATATTACTA
TAGATTCAGGACCCGGAGCTCGCTCGGTCCGTGTCGATCTTGCTCCTTTCACTTTAGTGGGAGCAACGACTCGATCAGGA
ATGCTAAGCGAACCTTTAAGAACACGCTTTGCTTTTAGTGCGAGACTTTCCTATTACTCGGATCAAGATCTAAAAGAGAT
TTTAGTCCGCTCCTCACATTTACTCGGAATCGAAGCTGACAGCTCCGCATTACTAGAAATTGCTAAGAGATCCCGAGGGA
CGCCACGACTGGCAAATCATCTTCTACGTTGGGTCAGAGATTTTGCTCAGATCCGAGAAGGAAACTGTATCAATGGGGAC
GTAGCAGAAAAAGCTTTGGCTATGCTATTAATAGATGATTGGGGATTGAATGAAATTGATATCAAACTTCTCACTACAAT
CATCGACTACTACCAAGGTGGTCCCGTTGGAATTAAAACCTTATCGGTAGCTGTGGGAGAAGATATCAAAACTCTTGAAG
ATGTTTATGAACCGTTTTTAATTTTAAAAGGTTTTATCAAAAAGACTCCCAGAGGCAGAATGGTAACACAACTTGCTTAC
GACCATTTAAAAAGACATGCAAAGAACTTATTGAGTTTAGGAGAAGGACAGTGA

Upstream 100 bases:

>100_bases
CTCGTGTACTATGCCGAGTATAACCGATCGGCGTTATCGATGAGAGTTTCAAAAAAATATAAAATCCACCTAAAGAAAAA
GCGATAGAGAAGGTTCGTAC

Downstream 100 bases:

>100_bases
AACTATTGAAAAACGTACTTTTAGGTCTTTTCTTCAGTATGAGTATCTCAGGATTCTCAGAAGTAAAGGTATCCGATACT
TTTGTGAAGCAGGATACTGT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTHQVAVLHQDKKFDVSLRPKGLEEFYGQHHLKERLDLFLCAALQRGEVPGHCLFFGPPGLGKTSLAHIVAYTVGKGLVL
ASGPQLIKPSDLLGLLTSLQEGDVFFIDEIHRMGKVAEEYLYSAMEDFKVDITIDSGPGARSVRVDLAPFTLVGATTRSG
MLSEPLRTRFAFSARLSYYSDQDLKEILVRSSHLLGIEADSSALLEIAKRSRGTPRLANHLLRWVRDFAQIREGNCINGD
VAEKALAMLLIDDWGLNEIDIKLLTTIIDYYQGGPVGIKTLSVAVGEDIKTLEDVYEPFLILKGFIKKTPRGRMVTQLAY
DHLKRHAKNLLSLGEGQ

Sequences:

>Translated_337_residues
MTHQVAVLHQDKKFDVSLRPKGLEEFYGQHHLKERLDLFLCAALQRGEVPGHCLFFGPPGLGKTSLAHIVAYTVGKGLVL
ASGPQLIKPSDLLGLLTSLQEGDVFFIDEIHRMGKVAEEYLYSAMEDFKVDITIDSGPGARSVRVDLAPFTLVGATTRSG
MLSEPLRTRFAFSARLSYYSDQDLKEILVRSSHLLGIEADSSALLEIAKRSRGTPRLANHLLRWVRDFAQIREGNCINGD
VAEKALAMLLIDDWGLNEIDIKLLTTIIDYYQGGPVGIKTLSVAVGEDIKTLEDVYEPFLILKGFIKKTPRGRMVTQLAY
DHLKRHAKNLLSLGEGQ
>Mature_336_residues
THQVAVLHQDKKFDVSLRPKGLEEFYGQHHLKERLDLFLCAALQRGEVPGHCLFFGPPGLGKTSLAHIVAYTVGKGLVLA
SGPQLIKPSDLLGLLTSLQEGDVFFIDEIHRMGKVAEEYLYSAMEDFKVDITIDSGPGARSVRVDLAPFTLVGATTRSGM
LSEPLRTRFAFSARLSYYSDQDLKEILVRSSHLLGIEADSSALLEIAKRSRGTPRLANHLLRWVRDFAQIREGNCINGDV
AEKALAMLLIDDWGLNEIDIKLLTTIIDYYQGGPVGIKTLSVAVGEDIKTLEDVYEPFLILKGFIKKTPRGRMVTQLAYD
HLKRHAKNLLSLGEGQ

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=309, Percent_Identity=51.1326860841424, Blast_Score=331, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_CHLPN (Q9Z8F3)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A81586
- PIR:   D72084
- PIR:   D86539
- RefSeq:   NP_224590.1
- RefSeq:   NP_300447.1
- RefSeq:   NP_444913.1
- RefSeq:   NP_876676.1
- ProteinModelPortal:   Q9Z8F3
- SMR:   Q9Z8F3
- GeneID:   1467083
- GeneID:   894883
- GeneID:   919210
- GeneID:   962853
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0365
- KEGG:   cpn:CPn0390
- KEGG:   cpt:CpB0402
- TIGR:   CP_0365
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   CPNE115711:CP_0365-MONOMER
- BioCyc:   CPNE115713:CPN0390-MONOMER
- BioCyc:   CPNE138677:CPJ0390-MONOMER
- BioCyc:   CPNE182082:CPB0402-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF00004 AAA; PF05491 RuvB_C

EC number: =3.6.4.12

Molecular weight: Translated: 37399; Mature: 37268

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHQVAVLHQDKKFDVSLRPKGLEEFYGQHHLKERLDLFLCAALQRGEVPGHCLFFGPPG
CCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
LGKTSLAHIVAYTVGKGLVLASGPQLIKPSDLLGLLTSLQEGDVFFIDEIHRMGKVAEEY
CCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
LYSAMEDFKVDITIDSGPGARSVRVDLAPFTLVGATTRSGMLSEPLRTRFAFSARLSYYS
HHHHHHCCEEEEEEECCCCCCEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
DQDLKEILVRSSHLLGIEADSSALLEIAKRSRGTPRLANHLLRWVRDFAQIREGNCINGD
CHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
VAEKALAMLLIDDWGLNEIDIKLLTTIIDYYQGGPVGIKTLSVAVGEDIKTLEDVYEPFL
HHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCHHHHHHHHHHHHH
ILKGFIKKTPRGRMVTQLAYDHLKRHAKNLLSLGEGQ
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
THQVAVLHQDKKFDVSLRPKGLEEFYGQHHLKERLDLFLCAALQRGEVPGHCLFFGPPG
CCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
LGKTSLAHIVAYTVGKGLVLASGPQLIKPSDLLGLLTSLQEGDVFFIDEIHRMGKVAEEY
CCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
LYSAMEDFKVDITIDSGPGARSVRVDLAPFTLVGATTRSGMLSEPLRTRFAFSARLSYYS
HHHHHHCCEEEEEEECCCCCCEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
DQDLKEILVRSSHLLGIEADSSALLEIAKRSRGTPRLANHLLRWVRDFAQIREGNCINGD
CHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
VAEKALAMLLIDDWGLNEIDIKLLTTIIDYYQGGPVGIKTLSVAVGEDIKTLEDVYEPFL
HHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCHHHHHHHHHHHHH
ILKGFIKKTPRGRMVTQLAYDHLKRHAKNLLSLGEGQ
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362