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Identifier: 16752511

GI number: 16752511

Start: 228331

End: 229128

Strand: Direct

Name: Not Available

Synonym: CP0222

Alternate gene names: 16752511

Gene position: 228331-229128 (Clockwise)

Preceding gene: 16752510

Following gene: 16752512

Centisome position: 18.57

GC content: 38.6

Gene sequence:

>798_bases
ATGGATTGCATAGGGAAACATAATCCTCTAGTTAAAGAGGCTCTAGCTTTAAAACGGTCTCGTTGTAGAAAAAGTTCTTG
GTTTCTAGTTGAAGGAGCCCGTGAGATTCAGAAAGCATTACGCACAGGCTACCTGTGTCAGCATGTTTTTTGTTCGACAC
ATCTTTCAGAAAAAGAAAAGGAATTTTTATATGAGTTAAAAAGAAATTCTACAAAGATTTTATATTGCTTAGACTCGACT
TTGGCTCAACTTTCTTTTAAAGAACATCACGATAGTTTCGTCGCGGTGATACAAAAGAGAGTGTGGAATAAAGAAGATTT
TTTGATCCAACGTAAAAATGCTCAGCCTTTCTATCTTATTATTGAGCAGGTGGAAAAACCTGGGAATGTCGGTGCTATTT
TAAGAATAGCTGATGGTGCTGGTGTTGACGGTGTGATTTTATGCAATCCTATTGTAGATTTGTACAATCCTAATGTGGTG
CGCTCTTCTTTAGGAGCTGTCTTTTCTCTTCCCATCCTCTCTATTTCAAGAGAGGAAGGGAAGGAGTTGTTCAAGCAGGA
AGGCTGGACTGTTTTTGTCACATCTCCTCGAGCTGAAACTATGTATTTTTCTAAAAATTATCTTGGTCCCACGGCTTTGG
TTTTTGGTTCAGAGAAAGATGGTTTGACTGAGGATTGGTTTTCTGAAGATTTTTCTGAAATCGCTCTTCCTATGTTAGGG
GAATCAGATTCTTTGAATCTCGCTACATCTGTAGCTGCTGTAGCTTATGAAGTTGTTCGTCAACGTTGGGTTAATTAG

Upstream 100 bases:

>100_bases
CTAGGCGTAGCGTGCCAACCCTTGTTTCTGAAGCGTGGTCTTGTGGGGAAAGTTTTTGTGGAGAAGGTGTGGGAGCCTTG
CCTTCTGGGAGTTTTGTTCA

Downstream 100 bases:

>100_bases
TACACCATTTTTCTTTTTGAGATCAAATTAATTATATTAGTTTTTTGTTTAACACAATTTTTTAATTAAGCTTTGTAAAC
TTGATTTTTAATCTTTTCTT

Product: spoU rRNA methylase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MDCIGKHNPLVKEALALKRSRCRKSSWFLVEGAREIQKALRTGYLCQHVFCSTHLSEKEKEFLYELKRNSTKILYCLDST
LAQLSFKEHHDSFVAVIQKRVWNKEDFLIQRKNAQPFYLIIEQVEKPGNVGAILRIADGAGVDGVILCNPIVDLYNPNVV
RSSLGAVFSLPILSISREEGKELFKQEGWTVFVTSPRAETMYFSKNYLGPTALVFGSEKDGLTEDWFSEDFSEIALPMLG
ESDSLNLATSVAAVAYEVVRQRWVN

Sequences:

>Translated_265_residues
MDCIGKHNPLVKEALALKRSRCRKSSWFLVEGAREIQKALRTGYLCQHVFCSTHLSEKEKEFLYELKRNSTKILYCLDST
LAQLSFKEHHDSFVAVIQKRVWNKEDFLIQRKNAQPFYLIIEQVEKPGNVGAILRIADGAGVDGVILCNPIVDLYNPNVV
RSSLGAVFSLPILSISREEGKELFKQEGWTVFVTSPRAETMYFSKNYLGPTALVFGSEKDGLTEDWFSEDFSEIALPMLG
ESDSLNLATSVAAVAYEVVRQRWVN
>Mature_265_residues
MDCIGKHNPLVKEALALKRSRCRKSSWFLVEGAREIQKALRTGYLCQHVFCSTHLSEKEKEFLYELKRNSTKILYCLDST
LAQLSFKEHHDSFVAVIQKRVWNKEDFLIQRKNAQPFYLIIEQVEKPGNVGAILRIADGAGVDGVILCNPIVDLYNPNVV
RSSLGAVFSLPILSISREEGKELFKQEGWTVFVTSPRAETMYFSKNYLGPTALVFGSEKDGLTEDWFSEDFSEIALPMLG
ESDSLNLATSVAAVAYEVVRQRWVN

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=150, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790083, Length=152, Percent_Identity=30.9210526315789, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30005; Mature: 30005

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCIGKHNPLVKEALALKRSRCRKSSWFLVEGAREIQKALRTGYLCQHVFCSTHLSEKEK
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
EFLYELKRNSTKILYCLDSTLAQLSFKEHHDSFVAVIQKRVWNKEDFLIQRKNAQPFYLI
HHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCEEE
IEQVEKPGNVGAILRIADGAGVDGVILCNPIVDLYNPNVVRSSLGAVFSLPILSISREEG
EEHHCCCCCCEEEEEEECCCCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
KELFKQEGWTVFVTSPRAETMYFSKNYLGPTALVFGSEKDGLTEDWFSEDFSEIALPMLG
HHHHHHCCCEEEEECCCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
ESDSLNLATSVAAVAYEVVRQRWVN
CCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDCIGKHNPLVKEALALKRSRCRKSSWFLVEGAREIQKALRTGYLCQHVFCSTHLSEKEK
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
EFLYELKRNSTKILYCLDSTLAQLSFKEHHDSFVAVIQKRVWNKEDFLIQRKNAQPFYLI
HHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCEEE
IEQVEKPGNVGAILRIADGAGVDGVILCNPIVDLYNPNVVRSSLGAVFSLPILSISREEG
EEHHCCCCCCEEEEEEECCCCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
KELFKQEGWTVFVTSPRAETMYFSKNYLGPTALVFGSEKDGLTEDWFSEDFSEIALPMLG
HHHHHHCCCEEEEECCCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
ESDSLNLATSVAAVAYEVVRQRWVN
CCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]