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The map label for this gene is ruvA

Identifier: 16752420

GI number: 16752420

Start: 127446

End: 128069

Strand: Direct

Name: ruvA

Synonym: CP0127

Alternate gene names: 16752420

Gene position: 127446-128069 (Clockwise)

Preceding gene: 16752419

Following gene: 16752421

Centisome position: 10.36

GC content: 41.35

Gene sequence:

>624_bases
ATGTACGACTATATTCGTGGAACACTGACCTATGTGCATACTGGTGCAATTGTTATAGAATGCCAAGGTATTGGCTACCA
TATTGCTATTACAGAACGTTGGGCAATAGAATGCATCAGGGCTTTACATCAAGACTTTCTCGTCTTCACTCATGTGATAT
TCCGTGAAACGGAACACTTGCTCTATGGGTTTCATTCTCGAGAAGAGCGAGAGTGTTTCCGTATTTTAATTTCTTTTTCT
GGAATAGGACCTAAGCTAGCCCTCGCGATTCTTAATGCGCTACCTTTAAAGGTACTATGTTCTGTAGTCCGATCTGAAGA
TATTCGTGCTTTAGCTTCCGTATCGGGAATTGGGAAAAAAACTGCTGAAAAACTCATGGTTGAGCTTAAACAAAAATTAC
CAGATTTACTTCCTCTAGACTCGAGAGTGGAGACCAGCCAAACACATACCACCTCTTCTTGCTTGGAGGAAGGCATTCAG
GCTTTAGCAGCTTTGGGTTATTCAAAAATTGCTGCTGAACGTATGATTGCCGAAGCAATCAAAGATCTCCCTGAGGGATC
TTCTTTAACAGATATCCTTCCTATCGCTCTGAAAAAGAATTTTTCAGGAGTGAACAAGGACTAG

Upstream 100 bases:

>100_bases
GAAGTTTTGCACCCTTCGAATGAAGATATTGCTGATGCCTTTGCACTTGCTATATGTCATACTCATGTAGCGCGTAGCCC
TCTTTGTGGAGTGAGATAAG

Downstream 100 bases:

>100_bases
ACTTTCCTAAAGATCATGCTATACTCCTCCTAAAATAAAACGATTTTATTTAAAGGAGATATGTATACACCATGGAACAA
ACGCTATCCATTATTAAACC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MYDYIRGTLTYVHTGAIVIECQGIGYHIAITERWAIECIRALHQDFLVFTHVIFRETEHLLYGFHSREERECFRILISFS
GIGPKLALAILNALPLKVLCSVVRSEDIRALASVSGIGKKTAEKLMVELKQKLPDLLPLDSRVETSQTHTTSSCLEEGIQ
ALAALGYSKIAAERMIAEAIKDLPEGSSLTDILPIALKKNFSGVNKD

Sequences:

>Translated_207_residues
MYDYIRGTLTYVHTGAIVIECQGIGYHIAITERWAIECIRALHQDFLVFTHVIFRETEHLLYGFHSREERECFRILISFS
GIGPKLALAILNALPLKVLCSVVRSEDIRALASVSGIGKKTAEKLMVELKQKLPDLLPLDSRVETSQTHTTSSCLEEGIQ
ALAALGYSKIAAERMIAEAIKDLPEGSSLTDILPIALKKNFSGVNKD
>Mature_207_residues
MYDYIRGTLTYVHTGAIVIECQGIGYHIAITERWAIECIRALHQDFLVFTHVIFRETEHLLYGFHSREERECFRILISFS
GIGPKLALAILNALPLKVLCSVVRSEDIRALASVSGIGKKTAEKLMVELKQKLPDLLPLDSRVETSQTHTTSSCLEEGIQ
ALAALGYSKIAAERMIAEAIKDLPEGSSLTDILPIALKKNFSGVNKD

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=198, Percent_Identity=37.3737373737374, Blast_Score=120, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_CHLPN (Q9Z7T4)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A86568
- PIR:   H72056
- RefSeq:   NP_224816.1
- RefSeq:   NP_300676.1
- RefSeq:   NP_444679.1
- RefSeq:   NP_876918.1
- ProteinModelPortal:   Q9Z7T4
- SMR:   Q9Z7T4
- GeneID:   1467325
- GeneID:   895411
- GeneID:   919384
- GeneID:   962994
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0127
- KEGG:   cpn:CPn0620
- KEGG:   cpt:CpB0646
- TIGR:   CP_0127
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- PhylomeDB:   Q9Z7T4
- ProtClustDB:   PRK00116
- BioCyc:   CPNE115711:CP_0127-MONOMER
- BioCyc:   CPNE115713:CPN0620-MONOMER
- BioCyc:   CPNE138677:CPJ0620-MONOMER
- BioCyc:   CPNE182082:CPB0646-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =3.6.4.12

Molecular weight: Translated: 22970; Mature: 22970

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDYIRGTLTYVHTGAIVIECQGIGYHIAITERWAIECIRALHQDFLVFTHVIFRETEHL
CCCHHHHHHHHEECCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYGFHSREERECFRILISFSGIGPKLALAILNALPLKVLCSVVRSEDIRALASVSGIGKK
HHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHH
TAEKLMVELKQKLPDLLPLDSRVETSQTHTTSSCLEEGIQALAALGYSKIAAERMIAEAI
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDLPEGSSLTDILPIALKKNFSGVNKD
HHCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MYDYIRGTLTYVHTGAIVIECQGIGYHIAITERWAIECIRALHQDFLVFTHVIFRETEHL
CCCHHHHHHHHEECCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYGFHSREERECFRILISFSGIGPKLALAILNALPLKVLCSVVRSEDIRALASVSGIGKK
HHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHH
TAEKLMVELKQKLPDLLPLDSRVETSQTHTTSSCLEEGIQALAALGYSKIAAERMIAEAI
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDLPEGSSLTDILPIALKKNFSGVNKD
HHCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362